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http://dx.doi.org/10.5352/JLS.2022.32.1.51

Production of PMA-induced MMP-2 and MMP-9 in the HT-1080 Fibrosarcoma Cell Line is Inhibited by Corydalis heterocarpa via the MAPK-related Pathway  

Yu, Ga Hyun (Marine Biotechnology Center for Pharmaceuticals and Foods, Silla University)
Karadeniz, Fatih (Department of Food and Nutrition, College of Medical and Life Sciences, Silla University)
Oh, Jung Hwan (Department of Food and Nutrition, College of Medical and Life Sciences, Silla University)
Kong, Chang-Suk (Marine Biotechnology Center for Pharmaceuticals and Foods, Silla University)
Publication Information
Journal of Life Science / v.32, no.1, 2022 , pp. 51-55 More about this Journal
Abstract
Matrix metalloproteinase (MMP) enzymes are responsible for the degradation and formation of the extracellular matrix (ECM), and overproduction of MMPs is observed in several diseases, such as cancer and asthma, that progress with metastatic characteristics. Natural products, especially phytochemicals, have been an important source of MMP inhibitors with reduced side effects. Although the majority of phytochemicals inhibit the enzymatic activity of MMPs, some suppress MMP production. In this context, the current study evaluated the potential of Corydalis heterocarpa, a halophyte with reported bioactivities, to inhibit MMP expression in PMA-stimulated HT-1080 cells. A crude C. heterocarpa extract was shown to decrease the mRNA and protein expression of MMP-2 and MMP-9 while increasing the endogenous MMP inhibitors TIMP-1 and TIMP-2 which regulate MMP expression in healthy tissues. In addition, our results show that the inhibitory effects of C. heterocarpa might occur through suppression of the phosphorylation of MAPK signaling, the upstream activator of MMP overexpression. In conclusion, C. heterocarpa is a potential source of antimetastatic compounds that might serve as lead molecules to develop novel MMP inhibitors.
Keywords
Corydalis heterocarpa; MAPK; MMP-9; TIMP-2;
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1 Murphy, G. 2011. Tissue inhibitors of metalloproteinases. Genome Biol. 12, 233.   DOI
2 Oh, J. H., Karadeniz, F., Lee, J. I., Kim, H. R., Seo, Y. and Kong, C. S. 2020. Antiphotoaging effect of (2'S)-columbia-netin from Corydalis heterocarpa in UVA-irradiated human dermal fibroblasts. Appl. Sci. 10, 2568.   DOI
3 Raeeszadeh-Sarmazdeh, M., Do, L. D. and Hritz, B. G. 2020. Metalloproteinases and their inhibitors: Potential for the development of new therapeutics. Cells 9, 1313.   DOI
4 Safranek, J., Pesta, M., Holubec, L., Kulda, V., Dreslerova, J., Vrzalova, J., Topolcan, O., Pesek, M., Finek, J. and Treska, V. 2009. Expression of MMP-7, MMP-9, TIMP-1 and TIMP-2 mRNA in lung tissue of patients with non-small cell lung cancer (NSCLC) and benign pulmonary disease. Anticancer Res. 29, 2513-2517.
5 Tombulturk, F. K., Soydas, T., Yaprak Sarac, E., Tuncdemir, M., Coskunpinar, E., Polat, E., Sirekbasan, S. and Kanigur-Sultuybek, G. 2019. Regulation of MMP 2 and MMP 9 expressions modulated by AP-1 (c-jun) in wound healing: improving role of Lucilia sericata in diabetic rats. Acta Diabetol. 56, 177-186.   DOI
6 Kong, C. S., Um, Y. R., Lee, J. I., Kim, Y. A., Yea, S. S. and Seo, Y. 2010. Constituents isolated from Glehnia littoralis suppress proliferations of human cancer cells and MMP expression in HT1080 cells. Food Chem. 120, 385-394.   DOI
7 Bae, M. J., Karadeniz, F., Oh, J. H., Yu, G. H., Jang, M. S., Nam, K. H., Seo, Y. and Kong, C. S. 2017. MMP-inhibitory effects of flavonoid glycosides from edible medicinal halophyte Limonium tetragonum. Evid. Based Complement. Altern. Med. 2017, 6750274.
8 Gonzalez-Avila, G., Sommer, B., Mendoza-Posada, D. A., Ramos, C., Garcia-Hernandez, A. A. and Falfan-Valencia, R. 2019. Matrix metalloproteinases participation in the metastatic process and their diagnostic and therapeutic applications in cancer. Crit. Rev. Oncol. Hematol. 137, 57-83.   DOI
9 Kang, K. H., Kong, C. S., Seo. Y., Kim, M. M. and Kim, S. K. 2009. Anti-inflammatory effect of coumarins isolated from Corydalis heterocarpa in HT-29 human colon carcinoma cells. Food Chem. Toxicol. 47, 2129-2134.   DOI
10 Kong, C. S., Kim, Y. A., Kim, M. M., Park, J. S., Kim, J. A., Kim, S. K., Lee, B. J., Nam, T. J. and Seo, Y. 2008. Flavonoid glycosides isolated from Salicornia herbacea inhibit matrix metalloproteinase in HT1080 cells. Toxicol. In Vitro 22, 1742-1748.   DOI
11 Kumar, G. B., Nair, B. G., Perry, J. J. P. and Martin, D. B. C. 2019. Recent insights into natural product inhibitors of matrix metalloproteinases. Medchemcomm 10, 2024-2037.   DOI
12 Malemud, C. 2006. Matrix metalloproteinases (MMPs) in health and disease: an overview. Front. Biosci. 11, 1696-1701.   DOI
13 Park, M. J., Kong, C. S. and Seo, Y. 2019. Inhibition of MMP2 and -9 by crude extracts and their solvent-partitioned fractions from the halophyte Atriplex gmelinii. Ocean Polar Res. 41, 79-88.
14 Anuar, N. N. M., Zulkafali, N. I. N. and Ugusman, A. 2021. Modulation of matrix metalloproteinases by plant-derived products. Curr. Cancer Drug Targets 21, 91-106.   DOI