• Journal of Plant Biotechnology
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• v.49 no.3
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• pp.240-249
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• 2022

Measuring chlorophyll fluorescence (CF) is a useful tool for assessing a plant's ability to tolerate abiotic stresses such as drought, waterlogging and high temperature. Korean ginseng is highly sensitive to water stress in paddy fields. To evaluate the possibility of non-destructively diagnosing waterlogging stress using chlorophyll fluorescence (CF) imaging techniques, we screened 57 ginseng accessions for waterlogging tolerance. To evaluate waterlogging tolerance among the 2-year-old Korean ginseng accessions, we treated ginseng plants with water stress for 25 days. The physiological disorder rate was characterized through visual assessment (an assigned score of 0-5). The physiological disorder rates of Geumjin, Geumsun and GS00-58 were lower than that of other accessions. In contrast, lines GS97-62, GS97-69 and GS98-1-5 were deemed susceptible. Root traits, chlorophyll content and the reduction rates decreased in most ginseng accessions. Further, these metrics were significantly lower in susceptible genotypes compared to resistant ones. All CF parameters showed a positive or negative response to waterlogging stress, and this response continuously increased over the treatment time among the genotypes. The CF parameter Fv/Fm was used to screen the 57 accessions, and the results showed clear differences in Fv/Fm between the susceptible and resistant genotypes. Susceptible genotypes had an especially low Fv/Fm value of less than 0.8, reflecting damage to the reaction center of photosystem II. It is concluded that Fv/Fm can be used as a CF parameter index for screening waterlogging stress tolerance in ginseng genotypes.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.21 no.1
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• pp.291-299
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• 2020

This study is based on the theory of salutogenesis, and investigates factors affecting the health status of radiologists who experience high stress and health problems in the rapidly changing medical environment. The study enrolled 379 radiologists working at hospitals in Daegu. A health structure model was established by applying the theory of salutogenesis. Sense of coherence had a direct effect on the health status of radiologists. Psychological stress, social support, and drinking were also influential factors, due to their mediating effect on the sense of coherence. Moreover, social support was also found to be lower when job stresses were higher. Hence, increased psychological stress resulted in lower levels of sense of coherence, social support and exercise. Taken together, results of this study indicate that effects on the sense of coherence have maximum influence on the health status of radiologists, and social support among general resistance resources is an important factor for enhancing sense of coherence. In addition to social support and health behaviors, researches involving various resources and the development of organizational strategies and health promotion intervention programs are required in future, to enable radiologists to cope with their stresses and improve health.

• Korean Journal of Plant Resources
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• v.16 no.3
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• pp.257-263
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• 2003

The NDK1 is an ubiquitous enzyme that transfer phosphate groups from triphosphate nucleoside diphosphates(NDPs) in eukaryotes and prokaryotes. We isolated and characterized a cDNA encoding a nucleoside diphosphate kinase 1(CNDK 1) in Codonopsis lanceolata. The CNDK 1 is 444bp long and open reading frame of 447bp with a deduced amino acid of 148 residue. The CNDK 1 has an ATP binding site in 12­16 residue and phosphohistidine intermediate in 115 residue of amino acid sequence. Although several NDK 1 genes have been cloned in plants, but little is known about the functional significance of this enzyme during plant growth and development. The CNDK 1 shows the identities to Arabidopsis thaliana (71％), Oryza sativa(75％), Glycine max (79％), Brassica rapa (77％), Mesembryanthemum crystallinum (85 ％), Spinacia oleracea (83％), Pisum sativum (82％). The CNDK 1 of C. laceolata have a closer relationship of Glycine max and Pisum sativum at the phylogenic analysis.

• Management & Information Systems Review
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• v.39 no.2
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• pp.1-15
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• 2020

Using information and communication technologies, many firms have increased their productivity. In resource based view, practical use of information and communication technologies is a process of increasing competitive advantage in uncertain environment. However, use of new information and communication technologies does not surely improve the productivity and work efficiency, but sometimes could be a factor to hinder firm performance. Technostress means adverse effect occurring in which the user of new information and communication technologies does not adapt to environment of new technologies. That is, Technostress is a negative impact resulted from using information and communication technologies. This study investigated the effect of technostress on counter-productivity. Unlike the previous studies, this study was made with the survey for firm employees of Daegu region, and both counter-productive work behavior and innovation resistance were used in this study. The empirical result means that technostress positively affects both counter-productive work behavior and innovation resistance. Because technostress increases both counter-productive work behavior and innovation resistance, systematic management for firm employees is needed in time adopting information and communication technologies.

• Journal of Life Science
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• v.31 no.9
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• pp.796-805
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• 2021

Hepatic endoplasmic reticulum (ER) stress contributes to the development of steatosis and insulin resistance. The components of unfolded protein response (UPR) regulate lipid metabolism. Recent studies have reported an association between ER stress and aberrant cellular lipid control; moreover, research has confirmed the involvement of sterol regulatory element-binding proteins (SREBPs)-the central regulators of lipid metabolism-in the process. However, the exact role of SREBPs in controlling lipid metabolism during ER stress and its contribution to fatty liver disease remain unknown. Here, we show that SREBP-1c deficiency protects against ER stress-induced hepatic steatosis in mice by regulating UPR, inflammation, and fatty acid oxidation. SREBP-1c directly regulated inositol-requiring kinase 1α (IRE1α) expression and mediated ER stress-induced tumor necrosis factor-α activation, leading to a reduction in expression of peroxisome proliferator-activated receptor γ coactivator 1-α and subsequent impairment of fatty acid oxidation. However, the genetic ablation of SREBP-1c prevented these events, alleviating hepatic inflammation and steatosis. Although the mechanism by which SREBP-1c deficiency prevents ER stress-induced inflammatory signaling remains to be elucidated, alteration of the IRE1α signal in SREBP-1c-depleted Kupffer cells might be involved in the signaling. Overall, the results suggest that SREBP-1c plays a crucial role in the regulation of UPR and inflammation in ER stress-induced hepatic steatosis.

• Korean Journal of Breeding Science
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• v.43 no.5
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• pp.457-463
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• 2011

Plant specific gene family, NAC (NAM, ATAF, and CUC) transcription factors have been characterized for their roles in plant growth, development, and stress tolerance. In this study, we isolated OsNAC69 gene and analyzed expression level by inoculation of bacterial leaf blight pathogen, Xanthomonas oryzae pv. oryzae (Xoo). NAC transcription factor family can be divided into five groups (I-V). On the basis of phylogenetic analysis, OsNAC69 was fall into group II. OsNAC69 was strongly induced 1 hr after infected with Xoo. To investigate its biological function in the rice, we constructed vector for overexpression in rice, and then generated transgenic rice lines. Gene expression of OsNAC69-overexpressed transgenic rice lines were analyzed by northern blot. Analysis of disease resistance to pathogen Xoo, nine OsNAC69-overexpressed transgenic rice lines showing high expression level of OsNAC69 were shown more resistant than wild type. These results suggest that OsNAC69 gene may play regulatory role during pathogen infection.

• Research in Community and Public Health Nursing
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• v.9 no.1
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• pp.21-29
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• 1998

This study was undertaken in order to examine the relationship of hardiness and health-promoting behavior and the effects of hardiness on stress-related physical symptoms. The subjects were 104 female nursing students of one college in Kongju. The instruments used for this study were a survey of general characteristics, hardiness(25 items), health-promoting behavior(44 items), and physical symptoms(35 items), Analysis of data was done by use of mean, Pearson correlation coefficient, stepwise regression and a hierarchical multiple regression with an SAS program. The results of this study are as follows. 1) Significant correlations between health -promoting behavior and subscales of hardiness, that is, control(r=-.35, P<.00l), commitment(r= -.29, P<.0l), and challenge(r= -.23, p<.05) were found. 2) Control was the highest factor predicting health -promoting behavior. 3) Main and buffering effects of hardiness on current physical symptoms were not found.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.67 no.1
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• pp.41-52
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• 2022

As a cultivar of Korean wheat, 'Keumgang' wheat variety has a fast growth period and can be grown stably. Hexaploid wheat (Triticum aestivum) has moderately high salt tolerance compared to tetraploid wheat (Triticum turgidum L.). However, the molecular mechanisms related to salt tolerance of hexaploid wheat have not been elucidated yet. In this study, the candidate genes related to salt tolerance were identified by investigating the genes that are differently expressed in Keumgang variety and examining salt tolerant mutation '2020-s1340.'. A total of 85,771,537 reads were obtained after quality filtering using NextSeq 500 Illumina sequencing technology. A total of 23,634,438 reads were aligned with the NCBI Campala Lr22a pseudomolecule v5 reference genome (Triticum aestivum). A total of 282 differentially expressed genes (DEGs) were identified in the two Triticum aestivum materials. These DEGs have functions, including salt tolerance related traits such as 'wall-associated receptor kinase-like 8', 'cytochrome P450', '6-phosphofructokinase 2'. In addition, the identified DEGs were classified into three categories, including biological process, molecular function, cellular component using gene ontology analysis. These DEGs were enriched significantly for terms such as the 'copper ion transport', 'oxidation-reduction process', 'alternative oxidase activity'. These results, which were obtained using RNA-seq analysis, will improve our understanding of salt tolerance of wheat. Moreover, this study will be a useful resource for breeding wheat varieties with improved salt tolerance using molecular breeding technology.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2010.10a
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• pp.23-23
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• 2010

전북 서해안 새만금 지역에 조성되는 간척지 면적 28,300ha의 30%인 8,400ha 정도가 농업용지로 사용되는 계획이 확정됨에 따라 해당 용지를 효율적으로 사용 할 수 있는 다양한 활용 방안이 요구되고 있다. 대단위 농업지구가 들어설 예정지인 새만금 광활 간척지는 네덜란드와 유사하게 토양의 대부분이 미사질 양토로 이루어져있어 적용 가능한 화훼류를 적절히 선발하고 적합한 재배기술을 도입한다면 대규모 화훼제배 단지로서의 성장 가능성이 충분할 것으로 판단된다. 따라서 전북 농업기술원에서는 2004년부터 7년에 걸쳐 새만금 간척지의 농업적 활용도 제고와 경관농업 육성을 통한 관광자원화 가능성을 확인하기 위하여 72종의 원예작물 적응시험을 실시하였으며 22종의 자생식물과 튤립, 아이리스, 수선화, 히아신스, 참나리 등 5종의 구근 화훼류를 적합 화훼류로 선발하였다. '08~09년에는 내염성과 저온 및 풍해 저항성이 있는 아펠톤, 골든아펠톤, 네그리타, 프랑소와즈, 키스네리스 등을 간척지적용 가능 주요 튤립 품종으로 선발 하였는데 선발된 품종들을 대상으로 높은 염농도 하에서의 체내축적 무기성분을 조사한 결과, K와 Mg은 증가되는 경향이었으나 Ca은 감소되었으며, Cl이온은 현저하게 증가하였다. 또한 튤립체내의 산도 및 전기전도도와 항산화효소의 활성이 증가하였는데 스트레스 반응결과 유해산소가 체내에 축적되고, 이를 중화시키기 위하여 수소이온을 소모함으로써 체내의 산도는 상승된 것으로 추정되며 염분함량이 높을수록 삼투압에 의하여 뿌리에서 흡수는 양분이 적은 반면 잎 줄기의 증산작용은 동일하기 때문에 상대적으로 체내의 농도가 높아져 전기전도도가 상승된 것으로 판단된다. 식물 스트레스의 지표물질로 사용되고 있는 proline 함량도 토양내의 염도가 상승되면서 증가되었으며 엽록소 함량은 감소되는 경향을 보였다.

• Proceedings of the Korean Society of Disaster Information Conference
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• 2017.11a
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• pp.123-124
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• 2017

최근에 장시간 근무로 인한 대중교통 운전자들의 부족한 휴식과 수면, 통증, 정신적 스트레스 등은 피로 누적를 초래하여 대중교통 사고의 주요 원인으로 대두되고 있다. 이에 2016년 산업통상자원부 산업융합촉진사업의 일환으로 대중교통 운전자용 다중 생체 신호측정 스마트 의류 및 관련 시스템이 개발중에 있으며 본 논문에는 현재 개발중인 스마트웨어러블 의류의 내세탁성능에 대한 성능 시험방법을 제안하고자 한다. 성능시험 방법은 국내외 생체신호측정 스마트 의류의 care label(취급표시사항)을 통하여 hand wash의 조건으로 세탁을 표시하고 있으나, 1년의 warranty를 위한 시험을 위해서는 오랜기간이 필요하기 때문에, 좀더 가혹한 조건으로 진행하여 시간을 줄이고, 고객의 빠른 시장출시를 지원하도록 시험방법을 제안하였다.