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http://dx.doi.org/10.5010/JPB.2022.49.3.240

Evaluation of waterlogging tolerance using chlorophyll fluorescence reaction in the seedlings of Korean ginseng (Panax ginseng C. A. Meyer) accessions  

Jee, Moo Geun (Ginseng & Medicinal Plant Research Institute, CNARES)
Hong, Young Ki (Ginseng & Medicinal Plant Research Institute, CNARES)
Kim, Sun Ick (Ginseng & Medicinal Plant Research Institute, CNARES)
Park, Yong Chan (Ginseng & Medicinal Plant Research Institute, CNARES)
Lee, Ka Soon (Ginseng & Medicinal Plant Research Institute, CNARES)
Jang, Won Suk (Ginseng & Medicinal Plant Research Institute, CNARES)
Kwon, A Reum (Ginseng & Medicinal Plant Research Institute, CNARES)
Seong, Bong Jae (Ginseng & Medicinal Plant Research Institute, CNARES)
Kim, Me-Sun (Department of Crop Science, Chungbuk National University)
Cho, Yong-Gu (Department of Crop Science, Chungbuk National University)
Publication Information
Journal of Plant Biotechnology / v.49, no.3, 2022 , pp. 240-249 More about this Journal
Abstract
Measuring chlorophyll fluorescence (CF) is a useful tool for assessing a plant's ability to tolerate abiotic stresses such as drought, waterlogging and high temperature. Korean ginseng is highly sensitive to water stress in paddy fields. To evaluate the possibility of non-destructively diagnosing waterlogging stress using chlorophyll fluorescence (CF) imaging techniques, we screened 57 ginseng accessions for waterlogging tolerance. To evaluate waterlogging tolerance among the 2-year-old Korean ginseng accessions, we treated ginseng plants with water stress for 25 days. The physiological disorder rate was characterized through visual assessment (an assigned score of 0-5). The physiological disorder rates of Geumjin, Geumsun and GS00-58 were lower than that of other accessions. In contrast, lines GS97-62, GS97-69 and GS98-1-5 were deemed susceptible. Root traits, chlorophyll content and the reduction rates decreased in most ginseng accessions. Further, these metrics were significantly lower in susceptible genotypes compared to resistant ones. All CF parameters showed a positive or negative response to waterlogging stress, and this response continuously increased over the treatment time among the genotypes. The CF parameter Fv/Fm was used to screen the 57 accessions, and the results showed clear differences in Fv/Fm between the susceptible and resistant genotypes. Susceptible genotypes had an especially low Fv/Fm value of less than 0.8, reflecting damage to the reaction center of photosystem II. It is concluded that Fv/Fm can be used as a CF parameter index for screening waterlogging stress tolerance in ginseng genotypes.
Keywords
Ginseng; Waterlogging; Genetic Resource; Genotype; Chlorophyll fluorescence (CF);
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Times Cited By KSCI : 4  (Citation Analysis)
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