• Title/Summary/Keyword: 성구

Search Result 600, Processing Time 0.038 seconds

Biosorption of Metal Ions by Seaweed Alginate, Polyguluronate, and Polymannuronate (알긴산, 폴리글루론산 및 폴리만뉴론산에 의한 금속이온의 흡착)

  • Jung, Dae-Young;Son, Chang-Woo;Kim, Sung-Koo;Kim, Yi-Joon;Chung, Chung-Han;Lee, Jin-Woo
    • Journal of Life Science
    • /
    • v.19 no.5
    • /
    • pp.553-560
    • /
    • 2009
  • Based on $P_{1/2}$ values, relative affinities of alginate, polyguluronate, and polymannuronate for metal ions are, in order, as follows; 1) seaweed alginate: $Cu^{2+}$ > $Cd^{2+}$ > $Pb^{2+}$ > $Fe^{3+}$ >> $Zn^{2+}$ > $Sr^{2+}$ > $Ca^{2+}$ > $Co^{2+}$ >> $Cr^{6+}$ > $Mn^{2+}$ >> $Hg^{2+}$, $Mg^{2+}$, $Rb^+$, 2) polyguluronate: $Cd^{2+}$ > $Cu^{2+}$ > $Pb^{2+}$ > $Fe^{3+}$ >> $Ca^{2+}$ > $Sr^{2+}$, $Zn^{2+}$, $Co^{2+}$ >> $Mn^{2+}$ > $Cr^{6+}$ >> $Hg^{2+}$, $Mg^{2+}$, $Rb^+$, and 3) polymannuronate: $Cd^{2+}$, $Cu^{2+}$ > $Fe^{3+}$ > $Pb^{2+}$ > $Ca^{2+}$ > $Zn^{2+}$ > $Sr^{2+}$ > $Co^{2+}$ > $Cr^{6+}$ >> $Mn^{2+}$ >> $Hg^{2+}$, $Mg^{2+}$, $Rb^+$. Amounts of the metal ions, $Cd^{2+}$, $Cu^{2+}$, $Fe^{3+}$, $Pb^{2+}$, and $Zn^{2+}$, bound to 1 g of seaweed alginate, were measured as $363.5{\pm}45.0$, $226.3{\pm}9.2$, $1,299.4{\pm}$81.3, 500.7${\pm}$27.7, and 165.9${\pm}$11.4 mg, respectively. Amounts of the metal ions, $Cd^{2+}$, $Cu^{2+}$, $Fe^{3+}$, $Pb^{2+}$, and $Zn^{2+}$, bound to 1g of polyguluronate, were 354.5${\pm}$26.5, 177.6${\pm}$8.7, 1,288.6${\pm}$60.1, 424.0${\pm}$7.4, and 140.2${\pm}$28.5 mg, respectively, whereas those bound to 1 g of polymannuronate were 329.0${\pm}$10.3, 206.9${\pm}$1.9, 1,635.6${\pm}$11.1, 419.8${\pm}$12.6, and 251.0${\pm}$49.1 mg, respectively. Due to its higher solubility than alginate and higher affinity for metal ions than polyguluronate, polymannuronate can be used for bioremediation or biosorption of toxic and/or noble metal ions.

Effect of Pine (Pinus densiflora) Needle Extracts on Synthesis of Collagen in Osteoblastic MC3T3-E1 Cells (적송잎 추출물이 조골세포의 collagen 합성에 미치는 영향)

  • Jeon, Min-Hee;Kim, Young-Kyoung;Park, Yong-Soo;Hwang, Hyun-Jung;Kim, Sung-Gu;Lee, Sang-Hyeon;Choi, In-Soon;Kim, Mi-Hyang
    • Journal of Life Science
    • /
    • v.20 no.4
    • /
    • pp.607-613
    • /
    • 2010
  • Osteoporosis is a disease involving a decrease in bone mineral density and an increased risk of fractures. The MC3T3-E1 pre-osteoblastic cell line is a well-accepted model of osteogenesis in vitro. Pine needles have long been used as a traditional health-promoting medicinal food in Korea. In this study, MTT assay, the alkaline phosphatase (ALP) activity and collagen synthesis of osteoblast cells were investigated to determine the effects of pine needle extracts on cell proliferation and differentiation. Pine needle extracts were prepared using hexane, ethanol and water. The effects of the pine needle extracts were examined by comparing the results with those of commercial agents, such as proanthocyanidin. The MC3T3-E1 cells exposed to proanthocyanidin showed increased proliferation in a concentration-dependent manner. The cells exposed to the hexane extract showed a similar increase in proliferation to that observed with proanthocyanidin. The hexane extract showed the highest ALP activity. Moreover, a supplement of pine needle extracts induced collagen synthesis in MC3T3-E1 cells. The pine needle extract produced the highest level of collagen synthesis at concentrations of $10{\sim}50\;{\mu}g/ml$. These results indicate that pine needle extracts have an anabolic effect on bone by promoting osteoblastic differentiation, and may be used in the treatment of common metabolic bone diseases.

Anti-Inflammatory and Antioxidant Effect of Astaxanthin Derived from Microalgae (미세조류 유래 astaxanthin의 항염증 및 항산화 효과)

  • Kwak, Tae-Won;Cha, Ji-Young;Lee, Chul-Won;Kim, Young-Min;Yoo, Byung-Hong;Kim, Sung-Gu;Kim, Jong-Myoung;Park, Seong-Ha;An, Won-Gun
    • Journal of Life Science
    • /
    • v.21 no.10
    • /
    • pp.1377-1384
    • /
    • 2011
  • Astaxanthin (ATX) is a red-orange carotenoid pigment that occurs naturally in a wide variety of living organisms. In this study we investigated the inhibitory effects of ATX on the induction of inducible nitric oxide synthase (iNOS), nitric oxide (NO), proinflammatory cytokines, nuclear factor-kappa B(NF-${\kappa}B$) and reactive oxygen species (ROS) in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. In addition, we tested the superoxide radical scavenging activity of ATX by scavenging assay. iNOS and NF-${\kappa}B$ expressions were determined by immunoblot analysis. Interleukin (IL)-6 and tumour necrosis factor-${\alpha}$ (TNF-${\alpha}$) were assayed by ELISA. NO production was monitored by measuring the amount of nitrite. ROS was examined by using the 2', 7'-Dichlorodihydrofluorescin diacetate (DCFH-DA) method. At a concentration of 100 ${\mu}M$, ATX inhibited the expression level of LPS-induced NF-${\kappa}B$, as well as the production of LPS-induced NO and proinflammatory cytokines (IL-6 and TNF-${\alpha}$), by suppressing iNOS expression. In particular, the maximal inhibition rate of IL-6 and TNF-${\alpha}$ production by ATX (100 ${\mu}M$) was 65.2----- and 21.2-----, respectively. In addition, ATX inhibited the LPS-induced transcriptional activity of NF-${\kappa}B$, and this was associated with suppressing the translocations of NF-${\kappa}B$ from the cytosol to the nucleus. Moreover, at various concentrations (25-100 ${\mu}M$), ATX inhibited the intracellular level of ROS. At a concentration of 5 mg/ml, the superoxide radical scavenging activity of ATX was 1.33 times higher than ${\alpha}$-tocopherol of the same concentration. These results showed that ATX inhibited the expression of iNOS and the production of NO and proinflammatory cytokines resulting from ROS production and NF-${\kappa}B$ activation in macrophages. Furthermore, ATX was found to be more effective in superoxide radical scavenging activities compared to ${\alpha}$-tocopherol. These findings are expected to strengthen the position of ATX as anti-inflammatory medicine and antioxidant.

A study about the relation between elev ated transaminase lev el and severity of viral lower respiratory tract infection in children (바이러스성 하기도 감염 환자에서 간기능 이상과 중증도와의 연관성에 관한 연구)

  • Lee, Chul Hee;Shin, Seon Hee;Lee, Jung Won;Sung, Tae Jung;Kim, Sung Goo;Lee, Kyu Man
    • Pediatric Infection and Vaccine
    • /
    • v.14 no.1
    • /
    • pp.47-54
    • /
    • 2007
  • Purpose : Viral respiratory tract infection is most common cause for admission to hospital in children. There are many cases with elevated transaminase level in patients with viral lower respiratory tract infection (LRTI). The aim of this study was to compare indexes of disease severity such as duration of assisted ventilation, length of hospital stay and Respiratory Distress Assessment Instrument (RDAI) score in children with viral LRTI with and without elevated transaminase levels and to determine the etiology related to elevated transaminase levels in this patients group. Methods : Virological analysis was done from respiratory specimens obtained from patients with LRTI admitted to Kangnam Sacred Heart Hospital from Jan. 2003 to Jun. 2005. Viral diagnosis was made by isolation of viruses employing HEp-2 cell culture from nasopharyngeal aspiration. Medical records of children were reviewed retrospectively. We compared age, sex, RDAI score, Respiratory Rate (RR) score and mean duration of hospital stay between patients with elevated transaminase levels (Patient Group) and patients with normal transaminase levels (Control Group). Results : Viruses were isolated from 181 children with LRTI. 16 cases were excluded according to criteria. 28 cases (17.0%) had elevated transaminase levels (Patient group) and 137 cases (83.0%) had normal transaminase levels (Control group). There were no significant difference in duration of fever, RR score, RDAI score, incidence of $O_2$ inhalation and duration of hospital stay between patient group and control group. We found 17 (60.7%) cases of RSV, 4 cases (14.3%) of parainfluenza, 4 cases (14.3%) of influenza B virus, 3 cases (10.7%) of adenovirus and 1 case (3.6%) of influenza A virus infection in patient group and 78 cases (56.9%) of RSV, 28 cases (20.4%) of parainfluenza virus, 13 cases (9.5%) of influenza A virus, 9 cases (6.6%) of influenza B virus, 6 cases (4.4%) of adenovirus and 3 cases (2.2%) of coxsackie virus infection in control group. Conclusion : There were 28 cases (17.0%) with elevated transaminase level among patients with virus isolated LRTI. There was no relation between elevated transaminase level and severity of disease. The viral etiologies in two groups were not significantly different. There was no significant difference of age distribution between two groups.

  • PDF

Evaluation of Water Quality Impacts of Forest Fragmentation at Doam-Dam Watershed using GIS-based Modeling System (GIS 기반의 모형을 이용한 도암댐 유역의 산림 파편화에 따른 수(水)환경 영향 평가)

  • Heo, Sung-Gu;Kim, Ki-Sung;Ahn, Jae-Hun;Yoon, Jong-Suk;Lim, Kyoungjae;Choi, Joongdae;Shin, Yong-Chul;Lyou, Chang-Won
    • Journal of the Korean Association of Geographic Information Studies
    • /
    • v.9 no.4
    • /
    • pp.81-94
    • /
    • 2006
  • The water quality impacts of forest fragmentation at the Doam-dam watershed were evaluated in this study. For this ends, the watershed scale model, Soil and Water Assessment Tool (SWAT) model was utilized. To exclude the effects of different magnitude and patterns in weather, the same weather data of 1985 was used because of significant differences in precipitation in year 1985 and 2000. The water quality impacts of forest fragmentation were analyzed temporarily and spatially because of its nature. The flow rates for Winter and Spring has increased with forest fragmentations by $8,366m^3/month$ and $72,763m^3/month$ in the S1 subwatershed, experiencing the most forest fragmentation within the Doam-dam watershed. For Summer and Fall, the flow rate has increased by $149,901m^3/month$ and $107,109m^3/month$, respectively. It is believed that increased flow rates contributed significant amounts of soil erosion and diffused nonpoint source pollutants into the receiving water bodies. With the forest fragmentation in the S1 watershed, the average sediment concentration values for Winter and Spring increased by 5.448mg/L and 13.354mg/L, respectively. It is believed that the agricultural area, which were forest before the forest fragmentation, are responsible for increased soil erosion and sediment yield during the spring thaw and snow melts. For Spring and Fall, the sediment concentration values increased by 20.680mg/L and 24.680mg/L, respectively. Compared with Winter and Spring, the increased precipitation during Summer and Fall contributed more soil erosion and increased sediment concentration value in the stream. Based on the results obtained from the analysis performed in this study, the stream flow and sediment concentration values has increased with forest fragmentation within the S1 subwatershed. These increased flow and soil erosion could contribute the eutrophication in the receiving water bodies. This results show that natural functionalities of the forest, such as flood control, soil erosion protection, and water quality improvement, can be easily lost with on-going forest fragmentation within the watershed. Thus, the minimize the negative impacts of forest fragmentation, comprehensive land use planning at watershed scale needs to be developed and implemented based on the results obtained in this research.

  • PDF

A STUDY ON THE TEMPERAMENTAL CHARACTERISTICS OF KOREAN CHILDREN USING TODDLER TEMPERAMENT SCALE (걸음마기 기질평가척도를 이용한 한국 아동의 기질 특성 연구)

  • Choi, Sung-Ku;Hong, Sung-Do;Son, Jung-Woo
    • Journal of the Korean Academy of Child and Adolescent Psychiatry
    • /
    • v.13 no.1
    • /
    • pp.163-176
    • /
    • 2002
  • Objectives:This study was designed to investigate the temperamental characteristics and the differences of temperamental characteristics in Korean children according to the sociodemographic and family environment factors using Toddler Temperament Scale(TTS). Methods:The samples consisted of 1,175 children who were attending twenty-five Samsung Child Care Centers nationwide. Both Korean version of TTS and child developmental questionnaire(designed by the Department of Psychiatry, Samsung Medical Center) were distributed to the parents of these children. Score of 9 temperamental categories was determined using the result of TTS, and determination of 5 temperamental clusters was conducted by the Fullard's criteria. Statistical analyses were performed according to the sex, birth order, existence of siblings, residential city, residential province, marital state of child's parents, and parental relationship to compare the scores of temperamental categories and the distribution of temperamental clusters. Results:The distribution of temperamental clusters was as follows;Easy 35.8%, Intermediate Low (IL) 33.1%, Intermediate High(IH) 11.1%, Slow-To-Warm-Up 6.3%, and Difficult 13.6%. Some of 9 temperamental categories were statistically different according to the sex, birth order, existence of siblings, residential city, residental province, marital state of child's parents, and parental relationship. From the viewpoint of 5 temperamental clusters, there were statistically more Easy and less Difficult children in good relation between each parent(p=.022). In spite of no statistical significance, the children in conditions of first-born, non-existence of siblings, middle or small residential city, Chung-Cheong province, married state of parent had a tendency to be easier to care. Conclusions:The toddler temperamental characteristics of Korean children showed some differences in several sociodemographic and family environment factors. We could confirm that the 'Goodness of Fit' was very important in child temperament.

  • PDF

Study on Resource Plants of the Mt. Geonji, Jeonju City (전주시 건지산 일대의 자원식물상 연구)

  • Oh, Hyun-Kyung;Beon, Mu-Sup;Lim, Seong-Gu;Park, Joon-Moh;Kim, Kae-Hwan
    • Korean Journal of Plant Resources
    • /
    • v.21 no.1
    • /
    • pp.73-82
    • /
    • 2008
  • The resource plants of the Mt. Geonji was listed 354 taxa; 92 families, 242 genera, 303 species, 48 varieties and 3 forms. 354 taxa listed consists of 205 taxa of edible plants(57.1%),234 taxa of medicinal plants(65.2%), 167 taxa of ornamental plants(46.5%) and 218 taxa of the others(60.7%). Specific plant species by floral region were total 22 taxa; Trapella sinensis var. antennifera in class IV, Iris ensata var. spontanea in Class II, 16 taxa(Salix glandulosa, Alnus hirsuta, Chrysosplenium flagelliferum, Mallotus japonicus, Ilex macropoda, Grewia biloba var. parviflora, Vaccinium oldhami, Lysimachia barystachys, Fraxinus mandshurica, etc.) in class I. The naturalized plants in this site were 12 families, 23 genera, 28 species, 2 varieties, 30 taxa(Bromus unioloides, Phytolacca americana, Oenothera erythrosepala, Ipomoea hederacea var. hederacea, Aster pilosus, Erechtites hieracifolia) and naturalization rate was 8.5% of all 354 taxa vascular plants. Wild plants disturbing ecosystem like Solanum carolinense and Ambrosia artemisiifolia var. elatior have been increasing. So, it needs continuing control and conservation measures on the plant ecosystem.

The effects of polylactic/polyglycolic copolymer (PLA/PGA) membrane on the healing of 1-wall intrabony defects in beagle dogs (성견 1면 골 결손부에서 Polylactic/polyglycolic acid copolymer (PLA/PGA) 흡수성 차단막의 치주 조직 재생에 대한 연구)

  • Kim, Sung-Koo;Hwang, Sung-Joon;Kim, Min-Kyoung;Kim, Chang-Sung;Lee, Doug-Youn;Lee, Yong-Keun;Cho, Kyoo-Sung;Chai, Jung-Kiu;Kim, Chong-Kwan;Choi, Seong-Ho
    • Journal of Periodontal and Implant Science
    • /
    • v.33 no.4
    • /
    • pp.599-613
    • /
    • 2003
  • The goal of periodontal treatment is regeneration of the periodontium. Bone graft and absorbable PLA/PGA membrane have been used for this purpose. In this study, 4${\times}$4mm 1-wall intrabony defects were surgically created bilaterally in the mandible of five male beagles. The control group went through a conventional flap operation, while the experimental group I was treated with absorbable PLA/PGA membranes only, group II was treated with absorbable membrane and calcium phosphate. The results are the following : 1. The defect height was 4.82${\pm}$0.45mm in the control group, 4.93${\pm}$0.79mm in the experimental I group, and 4.92${\pm}$0.62mm in the experimental II group. There was no statistically significant difference among 3 groups(P <0.05). 2. The amount of junctional epithelium migration was 30.90${\pm}$9.92% of the defect height in the control group, 39.16${\pm}$7.51% in the experimental I group, and 38.68${\pm}$12.22% in the experimental II group. There was no statistically significant difference among 3 groups(P <0.05). 3. The amount of connective tissue adhesion was 36.38${\pm}$9.03% in the control group, 14.73${\pm}$3.93% in experimental I group, and 27.87${\pm}$9.70% experimental II group. Experimental group I was a statistically significantly different from control group(P <0.05). 4. The amount of new cementum regeneration was 32.92${\pm}$10.51%, 50.04${\pm}$7.61%, and 39.62${\pm}$12.14% for the control, experimental I, and experimental II group respectively. Experimental group I was a statistically significantly different from control group(P<0.05). 5. The amount of new alveolar bone regeneration was 27.24${\pm}$7.49%, 40.75${\pm}$8.03%, and 36.47${\pm}$15.11% for the control, experimental I, and experimental II group respectively. Experimental group I was a statistically significantly different from control group(P <0.05). The results suggest that the use of PLA/PGA membrane in 1-wall intrabony defect of beagle dogs may promote periodontal regeneration. Further studies are required to determine their regeneration effects.

Denture wearers' recognition for their oral health status, denture cleansing methods, and insurance health system (의치사용자의 구강건강, 의치관리 및 의치건강보험 인식에 대한 실태 조사)

  • Kim, Sunjai;Kim, Seong-Kyun;Noh, Kwantae;Ahn, Su-Jin;Baik, Sang-Hyun;Kim, Jee-Hwan;Yoo, Dong-Ki;Kim, Kyoung-Rok;Kwon, Kung-Rock
    • The Journal of Korean Academy of Prosthodontics
    • /
    • v.56 no.4
    • /
    • pp.287-294
    • /
    • 2018
  • Purpose: The aim of the present investigation was to understand the incidence, prevalence of denture stomatitis in denture wearers, who were over 60 years old, and use the results for a fundamental data to promote public awareness about denture stomatitis and its education tools. Materials and methods: From August 21 to September 8 2017, 500 denture wearers, who were more than 60 years old in Seoul and 4 other metropolitan cities (Busan, Daegu, Gwangju, and Daejeon), received questionnaires. The questionnaires included denture wearers' demographic distributions, specific dental treatment experiences, discomforts with current dentures, their oral health conditions, and the method of denture cleansing. Results: Thirty-two percent of 500 denture wearers responded that they have healthy oral conditions. Two hundred and eight respondents (41.6%) were aware of denture stomatitis. Only 131 (26.2%) were informed about the removable denture covered by national health insurance and 327 (65.4%) of denture wearers were using the improper denture cleansing methods. Conclusion: A large number of denture wearers still do not recognize the importance of proper treatment for denture stomatitis, insurance covered denture treatments, and adequate denture cleansing methods.

Single Dose Oral Toxicity of Schisandrae Semen Essential Oil in ICR Mice (ICR 마우스를 이용한 오미자 종자 정유의 단회경구투여 독성시험)

  • Han, Min Ho;Kim, Joo Wan;Kim, Ki Young;Kim, Sung Goo;Yu, Gyeng Jin;Cho, Yong Bok;Hwang, Hye Jin;Kim, Byung Woo;Kim, Cheol Min;Choi, Yung Hyun
    • Journal of Life Science
    • /
    • v.24 no.2
    • /
    • pp.191-195
    • /
    • 2014
  • Essential oils extracted or purified from various plants have shown various beneficial effects. Seed parts of Schizandra chinensis Baillon (Schisandrae Semen) have been used as a traditional medicine for thousands of years in parts of Asia, including Korea, China, and Japan. However, the pharmacological mechanisms of essential oils purified from S. fructus (S. chinensis Baillon) remain largely unresolved. The aim of this study was to investigate the safety of Schisandrae Semen essential oil (SSeo) by a single- dose toxicity study in mice. SSeo was orally administered at a dose of 5,000 mg/kg in ICR mice. All animals were sacrificed after 14 days of treatment. After a single administration, mortality, clinical signs, body weight changes, and gross pathological findings were observed for 14 days. We also measured parameters of organ weight, clinical chemistry, and hematology. No toxicological change related to the test substance or mortality was observed after administration of a single oral dose of SSeo. There were no adverse effects on clinical signs, body weight, or organ weight and no gross pathological findings in any treatment group. The clinical chemistry and hematological parameters were within the normal ranges except total bilirubin. Therefore, the approximate lethal dose for oral administration of SSeo in mice was considered to be over 5,000 mg/kg. The results on the single-dose toxicity of SSeo indicate that it is not possible to reach oral dose levels related to death or dose levels with any harmful side effects.