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http://dx.doi.org/10.5352/JLS.2014.24.2.191

Single Dose Oral Toxicity of Schisandrae Semen Essential Oil in ICR Mice  

Han, Min Ho (Anti-Aging Research Center & Blue-Bio Industry RIC, Dongeui University)
Kim, Joo Wan (Research Institute, Bio-Port Korea INC, Marine Bio-industry Development Center)
Kim, Ki Young (Research Institute, Bio-Port Korea INC, Marine Bio-industry Development Center)
Kim, Sung Goo (Research Institute, Bio-Port Korea INC, Marine Bio-industry Development Center)
Yu, Gyeng Jin (Department of Biochemistry, Dongeui University College of Oriental Medicine)
Cho, Yong Bok (Department of Biochemistry, Dongeui University College of Oriental Medicine)
Hwang, Hye Jin (Anti-Aging Research Center & Blue-Bio Industry RIC, Dongeui University)
Kim, Byung Woo (Anti-Aging Research Center & Blue-Bio Industry RIC, Dongeui University)
Kim, Cheol Min (Department of Biochemistry, Busan National University College of Medicine)
Choi, Yung Hyun (Anti-Aging Research Center & Blue-Bio Industry RIC, Dongeui University)
Publication Information
Journal of Life Science / v.24, no.2, 2014 , pp. 191-195 More about this Journal
Abstract
Essential oils extracted or purified from various plants have shown various beneficial effects. Seed parts of Schizandra chinensis Baillon (Schisandrae Semen) have been used as a traditional medicine for thousands of years in parts of Asia, including Korea, China, and Japan. However, the pharmacological mechanisms of essential oils purified from S. fructus (S. chinensis Baillon) remain largely unresolved. The aim of this study was to investigate the safety of Schisandrae Semen essential oil (SSeo) by a single- dose toxicity study in mice. SSeo was orally administered at a dose of 5,000 mg/kg in ICR mice. All animals were sacrificed after 14 days of treatment. After a single administration, mortality, clinical signs, body weight changes, and gross pathological findings were observed for 14 days. We also measured parameters of organ weight, clinical chemistry, and hematology. No toxicological change related to the test substance or mortality was observed after administration of a single oral dose of SSeo. There were no adverse effects on clinical signs, body weight, or organ weight and no gross pathological findings in any treatment group. The clinical chemistry and hematological parameters were within the normal ranges except total bilirubin. Therefore, the approximate lethal dose for oral administration of SSeo in mice was considered to be over 5,000 mg/kg. The results on the single-dose toxicity of SSeo indicate that it is not possible to reach oral dose levels related to death or dose levels with any harmful side effects.
Keywords
ICR mice; safety; Schisandrae Semen essential oil; single dose oral toxicity;
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