• Korean Journal of Plant Resources
• /
• v.35 no.4
• /
• pp.417-427
• /
• 2022

In this study, we investigated in vitro immuno-stimulatory and anti-obesity activity of fruit (LIF), leaves (LIL) and stems (LIS) from Lonicera insularis Nakai in mouse macrophages RAW264.7 cells and mouse pre-adipocytes 3T3-L1 cells. LIF, LIL and LIS increased the production of immunostimulatory factors such as nitric oxide (NO), inducible nitric oxide synthase (iNOS), interleukin-1β (IL-1β), cyclooxygenase-2 (COX-2), tumor necrosis factor-α (TNF-α) and activated phagocytosis in RAW264.7 cells. Inhibition of toll-like receptor 2/4 (TLR2/4) partly blocked LIF, LIL and LIS mediated production of immunostimulatory factors. In addition, inhibition of mitogen-activated protein kinases (MAPK) signaling attenuated the production of immunostimulatory factors induced by LIF, LIL and LIS. Based on these results of this study, LIF, LIL and LIS is thought to activate macrophages the production of immunostimulatory factors and phagocytosis through toll-like receptor 2/4 (TLR2/4) and MAPKs signaling pathway. In anti-obesity study, LIF reduced the lipid accumulation in 3T3-L1 cells. LIF increased the protein phosphorylation expressions such as AMP-activated protein kinase (AMPK), hormone sensitive lipase (HSL), adipose triglyceride lipase (ATGL) related to the lipolysis of the adipocytes. In addition, LIF increased the expression of proteins involved in energy metabolism and brown adipose tissues differentiation such as peroxisome proliferator-activated receptor gamma coativator 1α (PGC-1α) and PR domain-containing16 (PRDM16). These results suggest that LIF is involved in lipid accumulation inhibition through expressing the proteins such as lipolysis and differentiation of white adipocytes to brown adipocytes.

• Korean Journal of Pharmacognosy
• /
• v.3 no.4
• /
• pp.217-225
• /
• 1972

Mt. Deok-Yu is situated on the border of Chun-Buk and Kyung-Nam Do, Korea. The peak of Dae Deok-yu (1608m) and Nam Deok-Yu (1508m) is the highest of Mt. Deok-yu range and it has many other high peaks. The plant of Mt. Deok-yu were collected from May 1964 to August 1972. The natural and cultivated medicinal plants include 98 families, 213 genera, 305 species, 53 varietes, 3 formas and 360 kinds.

• Korean Journal of Pharmacognosy
• /
• v.6 no.1
• /
• pp.23-27
• /
• 1975

To evaluate nutritional values and to detect pharmacologically active and antibacterial components of wild edible plants in Korea, 20 species were examined. 1) The essential free amino acid compositions of these plants were determined by TLC. The plants contained threonine, leucine, valine and methionine. 2) In antibacterial tests of 17 species the plants Hemerocallis sp. and Plantago asiatica showed an antibacterial activity against Staphylococcus, Bacillus, Eschericia, and Salmonella species. 4) Rutin. quercitrin, $k\ddot{a}mpferol$, chlorogenic acid and caffeic acid were also identified.

• Korean Journal of Plant Resources
• /
• v.32 no.5
• /
• pp.442-447
• /
• 2019

In this study, we evaluated the effect of Rodgersia podophylla leave extracts (RPL) on ${\beta}-catenin$ level in human cancer cells. RPL dose-dependently inhibited cell proliferation in SW480, A549, MDA-MB-231, PC-3 and AsPC-1 cells. RPL dramatically decreased ${\beta}-catenin$ protein level in all cancer cells. However, decreased level of ${\beta}-catenin$ mRNA expression was observed in A549 and AsPC-1 cells. In addition, RPL dramatically attenuated cyclin D1 mRNA expression in all cancer cells. MG132 decreased the downregulation of ${\beta}-catenin$ protein level induced by RPL in all cancer cells, while RPL-induced downregulation of ${\beta}-catenin$ was inhibited by the inhibition of $GSK-3{\beta}$ by LiCl in MDA-MB-231 cells. RPL phosphorylated ${\beta}-catenin$ and $GSK-3{\beta}$. In addition, the inhibition of $GSK-3{\beta}$ by LiCl attenuated RPL-induced ${\beta}-catenin$ phosphorylation. Based on these findings, RPL may be a potential candidate for the development of chemopreventive or therapeutic agents for human cancer.

• Korean Journal of Pharmacognosy
• /
• v.12 no.4
• /
• pp.221-226
• /
• 1981

Besides the emodin, three anthraquinone derivatives were isolated from the roots of Polygonum ciliinerve (Nakai) Owi which is indigenous exclusively to Korea. Two of them were identified as physcion and emodin-8-O-${\beta}$-D-glucoside by mp, uv, ir and nmr spectra. And the third was converted to physcion and sugar by hydrolysis. The quantitative analysis of free and combined form of emodin and physcion was done by the spectrophotometry.

• Korean Journal of Pharmacognosy
• /
• v.28 no.3
• /
• pp.156-161
• /
• 1997

For the search of hepatoprotective compounds from the folk medicines, 14 natural products which have been traditionally used as hepatoprotective drugs in Korea were extracted with methanol. The extracts were screened for the antioxidant activity on lipid peroxidation induced by Fenton reaction in rat homogenate and Ac2F cell toxicity by t-hydroperoxide. Dendrobium moniliforme and Castanea crenata were chosen for the further investigation and its therapeutic effects on the liver damage induced by carbon tetrachloride in rats were evaluated. Oral administration of the extracts reduced the aspartate aminotransferase(AST) and alanine aminotransferase(ALT) activities in the serum of the carbon tetrachloride intoxicated rat. And the treatment of the extracts prevented the decrease of aminopyrine N-demethylation and aniline hydroxylation activities of the carbon tetrachloride-intoxicated rat liver. These results suggest that oral administration of Dendrobium moniliforme and Castanea crenata is effective in recovering the liver function in $CCl_4-treated$ rats.

• Korean Journal of Pharmacognosy
• /
• v.6 no.1
• /
• pp.35-38
• /
• 1975

Attempts were made to investigate the pharmacological actions of Euonymus japonicae Cortex which is used as a substitute of Eucommiae Cortex. Practically the influence of the alcohol extract of the bark on the blood pressure response of rabbit, was examined, and the results are as follows. 1) The intravenous injections of the alcohol extracts of the bark decreased the blood pressure of rabbits to 4.6 11.1 and 34.2 mmHg respectively. 2) The intravenous injection of 10 mg/kg of the alcohol extract after intravenous injection of atropine 4 mg/kg or that of propranolol 2 mg/kg did not give any notable change to the blood pressure. 3) The intravenous injection of 10 mg/kg of the alcohol extract slightly decreased the raised blood pressure caused by the administration of acetylcholine $10^{-8}g/kg\;and\;10^{-7}g/kg$.

• Korean Journal of Pharmacognosy
• /
• v.6 no.1
• /
• pp.29-34
• /
• 1975

Morphological characteristics of the Eucommia barks produced in Korea and China and of the barks of Euonymus japonicus $T_{HUMBERGI}$ in Korea and Euonymus pellucidifolia $H_{AGATA}$ in Taiwan were compared. The similarity in morphological features between Eucommiae Cortex produced in Korea and that of China suggests that both plants are of same botanical origin. However, the size of tissues and cells indicates that there is only one difference due to warm climatic environment. The similarity and difference of the barks of Euonymus japonicus produced in Korea and Euonymus pellucidifolia produced in Taiwan comfirmed that the two plants belong to the same genus and that they are different species.

• Korean Journal of Pharmacognosy
• /
• v.6 no.1
• /
• pp.39-42
• /
• 1975

Influence of Eucommiae Cortex in Korea on the blood pressure of the rabbit was examined, 1) The intravenous injection of 3 mg/kg, 10 mg/kg and 30 mg/kg of the water extract to the rabbit decreased its blood pressure to $9.7\;{\pm}\;1.9,\;26.4\;{\pm}\;1.7\;and \;35.5\;{\pm}\;2.7\;mmHg$. respectively. 2) The intravenous injection of 10 mg/kg of the water extract did not show any remarkable influence to the blood pressure caused by the intravenous injection of acetylcholine $10^{-8}\;g/kg\; and \;10^{-7}\;g/kg$. 3) The intravenous injection of the water extract after the intravenous injection atropine 2mg/kg did not show any remarkable change to the blood pressure 4) The intravenous injection of the water extract after the intravenous injection of propranolol 2mg/kg slightly decreased the blood pressure.

• Korean Journal of Pharmacognosy
• /
• v.32 no.3 s.126
• /
• pp.193-199
• /
• 2001

The inhibitory effects of methanol extracts of 135 medicinal herbs on diacylglycerol acyltransferase (DGAT) activity were investigated. DGAT was partially purified from rat liver. Eleven kinds of methanol extracts of medicinal herbs including Evodiae Fructus showed a mild inhibitory effect with the concentration of $125\;{\mu}g/ml$ (above 40% inhibition). Six kinds of methanol extracts including Ephedrae Herba exhibited a weak inhibition. Among them, three kinds of butanol extracts (Sophorae Radix, Arecae Semen, Caesalpiniae Lignum) and the chloroform extracts of Evodiae Fructus showed significant inhibitory activities (above 60% inhibition) at the same concentration.