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Characterization of Heading- and Yield-related Gene Loci in the Cheongcheong/Nagdong Doubled Haploid Line using Rice QTLs (청청/낙동 배가반수체 집단에서 QTL을 통한 출수기와 수량관련 유전자좌 분석)

  • Jang, Yoon-Hee;Park, Jae-Ryoung;Kim, Kyung-Min
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.64 no.1
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    • pp.1-17
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    • 2019
  • A quantitative trait loci (QTL) analysis of traits related to heading and yield was performed develop rice cultivars that are both early maturing and panicle weight type varieties. Our analysis included 120 strains of the Cheongcheong Nagdong doubled haploid (CNDH) variety. An observational growth experiment was conducted to identify genetic agronomic traits of CNDH. Heading date, ten plant weight, moisture, thousand grain weight, and yield had a normal distribution based on the frequency distribution table of the observational growth data. The QTL analysis found one heading-related and nine yield-related QTLs. The LOD of 2.85 was the largest in QTLs for heading date (QHD), 5.39 in QTLs for ten plant weight (QTPW), 3.92 in QTLs for moisture (QM), 4.80 in QTLs for thousand grain weight (QTGW), and 3.7 in QTLs for yield (QY). Genomic analysis detected 58 candidate genes on chromosome 2, 3, 7, 8, and 10. Among those, we found Rcd1 protein and OsERF3 gene in QM, MtN3 and zinc finger protein genes in QTGW, and OsNAC3 protein gene in QY. If further analysis reveals the presence of genes related to water content, thousand grain weight or yield in the CNDH stains, we can develop a selection of varieties that will be capable of coping with climate change and will contribute to global food problems.

Acute Toxicities of Emulsifiable Concentrates and Granules of Valeriana fauriei Briquet and Alpinia galangal Swartz Essential Oils against Cyprinus carpio (길초근(Valeriana fauriei Briquet) 및 양강근(Alpinia galangal Swartz) 정유의 유제 및 입제제형의 잉어(Cyprinus carpio)에 대한 급성독성)

  • Jeon, Hwang-Ju;Kim, Kyeongnam;Kim, Yong-Chan;Lee, Sung-Eun
    • Korean Journal of Environmental Biology
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    • v.36 no.4
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    • pp.659-664
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    • 2018
  • In this study, two essential oils (EOs) extracted from Aleriana fauriei and Alpinia galangal were formulated as an emulsifiable concentrate (EC) and a granule. In the evaluation of their acute toxicity on fishes, Cyprinus carpio adults were used and the toxicities were determined in a static condition. The formulations were prepared from the essential oil extracted by three different methods namely steam distillation (SD), solvent extraction (SE) and supercritical fluid extraction (SFE). The acute toxicities were calculated using $LC_{50}$ values. Among EOs, only the EO extracted by solvent showed acute toxicities on carps. Some of the EC, EOs of Aleriana fauriei did not exhibit toxicity, while EOs from Alpinia galangal showed potent acute toxicities on carps. Among the granules, granules formulated with Aleriana fauriei EO extracted by SD method and Alpinia galangal EO extracted by SFE method showed acute toxicities on fishes. Nevertheless, $LC_{50}$ of ECs and granules formulated with all types of EOs in this study was higher than the fish toxicity level III for pesticides suggested by Korea Rural Development Administration. Furthermore, cytochrome P450 1A and glutathione S-transferase were confirmed as biomarkers in carps in response to the exposure to Alpinia galangal EO extracted by SD and SFE method, tracking Alpinia galangal EO in the aquatic environment.

Bio Toxicity Assessment and Kinetic Model of 6 Heavy Metals Using Luminous Bacteria (발광미생물을 이용한 중금속 6종의 생물독성 평가 및 모델링)

  • Kim, Ilho;Lee, Jaiyeop
    • Journal of the Korean Society of Urban Environment
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    • v.18 no.4
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    • pp.547-555
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    • 2018
  • In addition to North America and Europe, Korea is also responding to the toxic damage caused by the production and distribution of chemicals. Methods for assessing bio-toxicity of harmful substances have been widely introduced, but it is required of quantitative and speedy information for modeling. For 6 heavy metals, as zinc, copper, chrome, cadmium, mercury and lead, bio-toxicity assessment and kinetics model were constructed using Vibrio fischeri which is widely used luminous bacteria. The degree of luminescence activity and the toxicity of heavy metals were relative limunescence unit, RLU measured as by using a photomultiplier embedded device. The toxicity was assessed by the concentration levels giving under 20% lethality and lethal concentration, $EC_{50}$. In the results, the toxicity order were followed from mercury, lead, copper, chrome, zinc and cadmium. $EC_{{50},{\infty}}$ obtained by trends of $EC_{50}$ by time follows had highly linear agreement with main parameters of bio-toxicity modelling. The average error rates of the reproduced lethality obtained from DAM and TDM model on the basis of body residue, were 10.2% for mercury, lead, copper, chrome and 20.0 for the all 6 methals.

Mythical Symbolism through Meaning Action of Roland Barthes -Focus on Image Relationship of Silla Myth and Jeju Myth (롤랑 바르트 의미작용을 통한 우리나라 신화 상징체계 연구 -고대 신라신화와 제주신화의 이미지 관계성 중심으로)

  • Kang, Younsim
    • The Journal of the Korea Contents Association
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    • v.20 no.12
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    • pp.82-94
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    • 2020
  • Images play an important role in the symbolic system as they are connected with imagination through the association of language. Through history, we know that Korean people have been a people of strong spiritual unity and unity for thousands of years. I tried to study how the Korean people's unified mental symbol system was utilized and accomplished through mythological images. Our people are recognized as a people of white clothes because they are connected with white clothes, and modifiers such as the country of the east where the sun does not go down are connected with the sun. The Korean people have been handed down according to the times, such as the son of the sky, the Hongik man, the birch tree and the Gyerim of Silla, as a symbol of the myth of Gojoseon, and do not know when it became a country that loved the sun and whether brightness became a symbol. In relation to the spiritual symbolic system of our nation, the mythical image of Jeju musindo embedded in the shamanist ideology was reinterpreted through the meaning of Roland Bart to provide a basis for the study of the spiritual symbolic system of our nation.

Ecotoxicity Evaluation of PFCs using Marine Invertebrate, Sea Urchin (Mesocentrotus nudus) (둥근성게(Mesocentrotus nudus)를 이용한 과불화화합물의 생태독성평가)

  • Choi, Hoon;Lee, Ju-Wook;Lee, Seung-Min;Jeon, Hyung-Ju;Heo, Seung;Hwang, Un-Ki
    • Journal of Marine Life Science
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    • v.6 no.2
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    • pp.80-87
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    • 2021
  • In this study, the toxic effects of PFOA and PFOS potassium salt on Mesocentrotus nudus using 10 min-fertilization rate and 48 h-normal embryogenesis were confirmed through the calculation of toxicity values such as Non-observed effective concentration, Low-observed effective concentration, and 50% of effective concentration. The case of 10 min-fertilization rate and 48 h-normal embryogenesis showed the concentration-dependent reduction pattern when exposed to PFOA and PFOS potassium salt, in tested concentration, respectively. The EC50 values of 10 min-fertilization rates for PFOA and PFOS potassium salt were 1346.43 mg/l and 536.18 mg/l, respectively, and the EC50 values of 48 h-normal embryogenesis were 42.67 mg/l and 17.81 mg/l, respectively. Both toxicity test methods showed high toxicity sensitivity to PFOS potassium salt. Recent studies have shown that the concentration of PFOA and PFOS in the marine environment has continuously decreased, and it is not enough to show acute toxicity to sea urchin. However, PFOA and PFOS have a very long half-life and can accumulate throughout the life of marine life, so it is still observed at a high concentration in shellfish. Therefore, a study on chronic toxicity through the whole-life cycle of marine organisms in coastal environments should be needed.

Relations of Five Color Preference with Chinese Body Constitution of Residents in Seoul Area (서울지역 주민의 중의(中醫) 체질(體質)별 오색(五色)선호도와의 연관성)

  • Ahn, Ji-Hye
    • Journal of Naturopathy
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    • v.9 no.1
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    • pp.13-21
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    • 2020
  • Purpose: The purpose of this study examined the distribution of constitution(Con) types by examining the Chinese constitution table questionnaire with 594 Seoul citizens. It investigated the preferences of five colors by the Con. It then was to explore the validity of the test and the five colors according to the Con. Methods: We used the CC table questionnaire and surveyed by using a cardinal color. Results: The Con type distributions include yangweak(31.4%), yin-weak, gall-humidity, harmony, qi-weak, qi-gloom, specific, and extravasate in order. The lack of statistical significance also indicates that the CC test is not appropriate for Seoul citizens. The order of five preferences is 31% in blue, 25.3% in yellow, 18% in white, 17.7% in red, and 8% in black. Blue was the most preferred and black the least. There were differences in the distribution of five colors, but they were not statistically significant. In five viscera diseases of the yang-weak con, the stomach was more than half(50.3%). The highest preferred color was blue (30.9%). This difference was significant(p <.01). It evaluated that this influences the preference of five colors by five diseases. Conclusions: The constitution of Seoulian in the CC survey distributed into nine categories. There was a difference in preference of five colors by the con. However, it was not significant. This indicates that the Chinese con table is not suitable for Seoulian. However, the results are to be primary data for research in this field.

Research for Intestinal Mucosal Immunity Induced by Salmonella enteritidis Infection (Salmonella enteritidis 감염에 의해 장내 점막에서 유도되는 면역반응에 관한 연구)

  • Lee, Kang-Hee;Lee, Se-Hui;Yang, Jin-Young
    • Journal of Life Science
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    • v.32 no.1
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    • pp.36-43
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    • 2022
  • Mucosal immunity is a well-designed defense system that builds precise and dynamic relationships against pathogens, and the gastrointestinal tract is the most important organ with this system, acting as a guardian at the forefront of its activity. Salmonella spp. cause food poisoning, entering the body orally and mainly invading the Peyer's patches of the small intestine. Although Salmonella strains share similar mechanisms for inducing innate immunity, different serotypes may have different effects on the intestinal mucosa due to host specificities and pathogenicity. In this study, we evaluated the effects of Salmonella enteritidis infections in mouse intestine and observed significantly reduced dose-dependent survival rates in a challenge test. Flow cytometry data showed no significant differences in intestinal immune cell populations, although histology indicated increased mucin production and decreased goblet cell counts in the Salmonella-treated groups. Furthermore, Claudin expression was significantly decreased in the samples with Salmonella. To investigate the relationship between S. enteritidis infection and inflammatory response, dextran sodium sulfate (DSS) was administered after infection and the results indicate lower survival rate after DSS treatment. In conclusion, we were able to identify the optimal concentration of S. enteritidis to modulate the intestinal mucosal immunity of mice and inflammatory response.

Comparative Analysis of Callus Induction and Plant Regeneration Rates Using One-step and Two-step Cultures for Rice Anther Cultivation (벼 약배양 1단계 및 2단계 배양을 이용한 캘러스 유도 및 식물 재부화율 비교 분석)

  • Park, Young-hie
    • Journal of Life Science
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    • v.31 no.4
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    • pp.385-388
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    • 2021
  • Anther cultivation for crop breeding is a method of rapid production of homozygosities by greatly reducing the time required for at least six generations to develop new varieties using conventional breeding methods. This technique of producing anther culture provides an opportunity to obtain more green plants from a methodological point of view, and the techniques that save time and effort in anther culture are also important because they increase the efficiency of culture. This study compared the callus induction rate and green plant regeneration rate of a one-step and a two-step culture that differ in their culture media and culture methods. One-step culture allows callus induction and plant regeneration in one medium, whereas two-step culture requires induction and plant regeneration in two different media. In this study, we compared the callus induction and plant regeneration rates of rice anthers as one-step and two-step cultures. The callus formation rate was 13.0% for one-step cultures and 8.6% for two-step cultures, so the rate was 4.4% higher for one-step cultures than for two-step cultures. The plant regeneration rate was 1.0% in one-step cultures and 3.0% in two-step cultures, so the regeneration rate was three times higher for the two-step cultures than for one-step cultures. This suggests that the two-step cultures are more efficient than the one-step cultures for haploid production.

Effect of Particle Size of Granular Nitrofen (TOK G) on the Leaf Burning in Rice Plants (Nitrofen입제(TOK G)의 입도가 수도의 약반형성에 미치는 영향)

  • Park, J.K.;Cho, Y.W.;Kwon, Y.W.
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.22 no.2
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    • pp.121-127
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    • 1977
  • Leaf burning due to nitrofenapplication was characterized as brown color spot appearing on the leaf sheath and blade. The spots predominantly appeared around 5-7 cm high above soil surface regardless of different particle size, formulation, application method, or water level. Magnitude and frequency of burn spots increased somewhat as the granular size gets finer, by higher water level, or in transplanted rice compared to directly sown rice when applied at 7 leaf-stage, but there was no indication of increase in leaf burning due to clinging of floating dust to rice plant. When collections from local distributor's stock of TOK 7G, a granular formulation of nitrofen, were analyzed for particle size composition, 98.9% by weight ranged between 9 to 35 meshes, and only about 0.08% by weight was of particles finer than 100 mesh.

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Possibility of Using Landfill Coal Ash as CLSM Material for Emergency Restoration of Ground and Road Joint Parts (지반 및 도로 공동부의 긴급복구용 CLSM 재료로 매립 석탄저회 활용 가능성)

  • Jin-Man Kim;Sang-Chul Shin;Kyoung-Nam Min;Ha-Seog Kim
    • Journal of the Korean Recycled Construction Resources Institute
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    • v.11 no.1
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    • pp.55-61
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    • 2023
  • This study aims to develop CLSM fill material for emergency restoration using landfill coal ash. As a result of examining physical properties such as particle size distribution and fines content of landfill coal ash, bottom ash, fly ash, and general soil were mixed, and SP was found to have a density of 2.03 and a residual particle pass rate of 7.8 %. CLSM materials that secure fluidity in unit quantities without using chemical admixtures such as glidants and water reducing agents have a high risk of material separation due to bleeding. As a result of this experiment, it was found that the bleeding ratio did not satisfy the standard in the case of the specimen with a large amount of fly ash and a lot of addition of mixing water. As a result of the compressive strength test, the strength development of 0.5 MPa or more for 4 hours was found to be satisfactory for the specimens using hemihydrate gypsum with a unit binder amount of 200 or more, and the remaining gypsum showed poor strength development. Although it is judged that landfill coal ash can be used as a CLSM material, it is necessary to identify and apply the physical and chemical characteristics of coal ash buried in the ash treatment plant of each power generation company.