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http://dx.doi.org/10.5352/JLS.2021.31.4.385

Comparative Analysis of Callus Induction and Plant Regeneration Rates Using One-step and Two-step Cultures for Rice Anther Cultivation  

Park, Young-hie (Department of Agricultural Sciences, Korea National Open University)
Publication Information
Journal of Life Science / v.31, no.4, 2021 , pp. 385-388 More about this Journal
Abstract
Anther cultivation for crop breeding is a method of rapid production of homozygosities by greatly reducing the time required for at least six generations to develop new varieties using conventional breeding methods. This technique of producing anther culture provides an opportunity to obtain more green plants from a methodological point of view, and the techniques that save time and effort in anther culture are also important because they increase the efficiency of culture. This study compared the callus induction rate and green plant regeneration rate of a one-step and a two-step culture that differ in their culture media and culture methods. One-step culture allows callus induction and plant regeneration in one medium, whereas two-step culture requires induction and plant regeneration in two different media. In this study, we compared the callus induction and plant regeneration rates of rice anthers as one-step and two-step cultures. The callus formation rate was 13.0% for one-step cultures and 8.6% for two-step cultures, so the rate was 4.4% higher for one-step cultures than for two-step cultures. The plant regeneration rate was 1.0% in one-step cultures and 3.0% in two-step cultures, so the regeneration rate was three times higher for the two-step cultures than for one-step cultures. This suggests that the two-step cultures are more efficient than the one-step cultures for haploid production.
Keywords
Anther; breeding; callus; crop; medium;
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1 Chen, Q. F., Wang C. L., Lu Y. M., Shen M., Afza, R., Duren, M. V. and Brunner, H. 2001. Anther culture in connection with induced mutations for rice improvement. Euphytica 120, 401-408.   DOI
2 Chung, G. S. and Sohn, J. K. 1996. Anther culture technology in rice. In Management Biotechnology 1-9.
3 He, T., Yang, Y., Tu, S. B., Yu, M. Q. and Li, X. F. 2006 Selection of interspecific hybrids for anther culture of indica rice. Plant Cell Tissue Organ Cult. 86, 271-277.   DOI
4 Moon, H. P., Cho, S. Y., Son, Y. H., Jun, B. T., Lim, M. S., Choi, H. C. and Chung, G. S. 1986. An anther-derived new high quality and high yield rice variety" Hwaseongbyeo". Research Reports of the Rural Development Administration-Crops (Korea R.)
5 Yi, G. H., Won, Y. J., Ko, J. M., Park, H. M., Cho, J. H., Oh, B. G. and Nam, M. H. 2003. Effects of cold shock pretreatment and carbohydrate sources on anther culture of rice. Plant Biotechnol. J. 30, 369-373.
6 Henry, Y., Vain, P. and De, Buyser, J. 1994 Genetic analysis of in vitro plant tissue culture responses and regeneration capacities. Euphytica 79, 45-58.   DOI
7 Kim, K. M., Nam, W. L., Kwon, Y. S. and Sohn, J. K. 2004. Development of doubled-haploid population and construction of genetic map using SSR makers in rice. Plant Biotechnol. J. 31, 179-184.
8 Liang, G. H., Xu, A. and Tang, H. 1987. Direct generation of wheat haploids via anther culture 1. Crop Sci. 27, 336-339.   DOI