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http://dx.doi.org/10.33768/ksue.2018.18.4.547

Bio Toxicity Assessment and Kinetic Model of 6 Heavy Metals Using Luminous Bacteria  

Kim, Ilho (Environmental and Plant Engineering Research Institute, Korea Institute of Civil Engineering and Building Technology)
Lee, Jaiyeop (Environmental and Plant Engineering Research Institute, Korea Institute of Civil Engineering and Building Technology)
Publication Information
Journal of the Korean Society of Urban Environment / v.18, no.4, 2018 , pp. 547-555 More about this Journal
Abstract
In addition to North America and Europe, Korea is also responding to the toxic damage caused by the production and distribution of chemicals. Methods for assessing bio-toxicity of harmful substances have been widely introduced, but it is required of quantitative and speedy information for modeling. For 6 heavy metals, as zinc, copper, chrome, cadmium, mercury and lead, bio-toxicity assessment and kinetics model were constructed using Vibrio fischeri which is widely used luminous bacteria. The degree of luminescence activity and the toxicity of heavy metals were relative limunescence unit, RLU measured as by using a photomultiplier embedded device. The toxicity was assessed by the concentration levels giving under 20% lethality and lethal concentration, $EC_{50}$. In the results, the toxicity order were followed from mercury, lead, copper, chrome, zinc and cadmium. $EC_{{50},{\infty}}$ obtained by trends of $EC_{50}$ by time follows had highly linear agreement with main parameters of bio-toxicity modelling. The average error rates of the reproduced lethality obtained from DAM and TDM model on the basis of body residue, were 10.2% for mercury, lead, copper, chrome and 20.0 for the all 6 methals.
Keywords
bio-toxicity assessment; luminous bacteria; heavy metal; bio-toxicity modeling; lethal concentration 50;
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  • Reference
1 ISO, ISO 11348-3, "Water quality-Determination of the inhibitory effect of water samples on the light emission of Vibrio fischeri (Luminescent bacteria test)-Part 3: Method using freeze-dried bacteria," (2007).
2 Hastings, J. W. and Nealson, K. H., "Bacterial Bioluminescence," Ann. Rev. Microbiol., 31, 549-595 (1977).   DOI
3 Lee, W. M., Kim, J. S., Kim, I. H., Kim, S. G., and Yoon, Y. H. "Toxic Interactions of Perfluorinated Compounds (PFCs) with Heavy Metals Using Vibrio fischeri," J. Kor. Soc. Environ. Eng., 36(2), 119-126 (2014).   DOI
4 Lee, J. H., "Toxicokinetic and Toxicodynamic Models for Ecological Risk Assessment," J. Environ. Toxicol. 24(2), 79-93 (2009).
5 Ashauer, R. and Brown, C. D., "Toxicodynamic Assumptions in Ecotoxicological Hazard Models," Environmental Toxicology and Chemistry, 27(8), 1817-1821 (2008).   DOI
6 Ashauer, R., Boxall A. B. A., and Brown, C. D., "Modeling Combined Effects of Pulsed Exposure to Carbaryl and Chlorpyrifos on Gammarus Pulex," Environ. Sci. & Technol., 41(15), 5535-5541 (2007).   DOI
7 De Keyser, W., Gevaert, V., Verdonck, F., Nopens, I., De Baets, B., Vanrolleghem, P. A., Mikkelsen, P. S., and Benedetti, L., "Combining multimedia models with integrated urban water system models for micropollutants," Water Science & Technology, 62(7), 1614-1622 (2010).   DOI
8 EPA, "Benchmark Dose Model Executables," http://www.epa.gov/ncea/bmds (2015).
9 Korea Ministry of Environment (MOE), "Chemical Materials Management System Safe and Beneficial for Public," Environmental Policy, 5 (2013).
10 Lee, J. Y., Lee, H. D., and Kim, I. H, "Bio-Toxicity Assessment of PFOA and PFOS to Vibrio Fischeri by Photomultiplier Tube," International Journal of Multimedia and Ubiquitous Engineering, 10(10), 247-258 (2015).
11 Korea Ministry of Environment (MOE), "Study on Introduction Plan of Integrated Toxicity Control System for Water Quality Toxic Substance(III)," (2005).
12 National Institute of Environmental Research (Korea), "Experimental Methods and Operation Guides and for Bio-toxicity : Revised Ver.," 2015. 12.
13 Korea Ministry of Environment (MOE), "Environmenal Policy Basic Law," (2017).