• Journal of Korea Technology Innovation Society
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• v.20 no.2
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• pp.430-457
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• 2017

The purpose of this study is to present meaningful information and policy implications concerning the determinants of royalties of technology transfer to stakeholder. To identify key determinants of royalties in technology transfer, this study conducted AHP survey analysis (Survey period: 01/09~31/10, 2016) of 96 government-funded research centers and 85 companies which were participants of the R&D project "Next Generation BioGreen21" of R.D.A in the "N" center from 2011 to 2015. Research results show that both parties acknowledge 'Technical considerations for determining the profitability of the technologies' and 'The interest and willingness of the management group' as critical factors for the determinants of royalties. The difference of each party is that private companies acknowledge 'Available budget plan' as a critical factor while the government-funded research centers value 'Market competitiveness'. These findings suggest four main policy implications which are the investigation of technological demands reflecting specific needs of industrial sites, the diversification of royalty payments for private companies, the differentiated research evaluation system for the purpose of technology transfer and the planning of public R&D project reflecting research time span of private companies.

• Journal of Plant Biotechnology
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• v.42 no.1
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• pp.34-42
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• 2015

This study was conducted to investigate the productivity of biomass and antioxidant compounds in Polygonum multiflorum by culturing explants in air-lift bioreactor containing Murashige and Skoog (MS) medium, by adding different concentrations of auxins [indole-3-butyric acid (IBA) and naphthalene acetic acid (NAA)], sucrose, methyl jasmonate (MeJA), and salicylic acid (SA). Results of this study revealed that the explants culturing on the medium supplemented with $9.84{\mu}M$ IBA and 50 g/L sucrose were observed to have higher productivity of biomass and bioactive compound than other treatments used. Thus, we expect that these results will be helpful for large-scale production of biomass and antioxidant compounds from Polygonum multiflorum.

• Journal of the Korean Wood Science and Technology
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• v.38 no.6
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• pp.507-517
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• 2010

This study estimated the amount of carbon stocks in harvested wood products (HWP) using accounting approaches suggested by 2006 IPCC guidelines and analyzed the impacts of different approaches on national greenhouse gas inventory and the forest sector in Korea. The change in carbon stocks was calculated at the level of semi-finished wood products, which cover sawnwood, wood-based panels, other industrial wood, paper and paperboard. An estimation of the changes in carbon stocks in HWP in use for the period 1970~2008 varied between -9,023 Gg $CO_2$/yr and 4,052 Gg $CO_2$/yr depending on the accounting approach used. The stock-change approach provided the most favorable results because Korea was a net importer of wood products. However, each approach generates different impacts on harvest, trade, the use of wood for energy production and recycling. When deciding its position on accounting approach, thus, the Government should consider future direction of national forest policies as well as the effect on national greenhouse gas inventory for the minimization of negative impacts resulting from its selection.

• Journal of Food Hygiene and Safety
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• v.25 no.4
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• pp.376-387
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• 2010

Recently, speedy, convenient and easy detection technologies have been developed rapidly and on the contrary, studies on development of traditional detectors applying biochemical characteristics has gradually been decreased. This review examined trend in current studies on detection of food-borne pathogenic microorganisms in the fields of selective media, immuno-assay, Polymerase Chain Reaction (PCR), microarray, terahertz spectroscopy & imagination and so on. Most traditional methods to detect the organisms from food matrix rely on selective media and such a method have disadvantages like long time requirement and distinguishing one species only from each selective medium although they are highly economical. Various new convenient methods such as Enzyme Linked Immuno-sorbent Assay (ELISA), paper-strip kit, fluoroimmunoassay etc. have been developed. The most ideal method for detecting food-borne pathogenic microorganisms in foods should be accurate, convenient, rapid and economical. Additionally, it is needed that capabilities of quantitative analysis and automation to be applied to industries.

• Journal of Life Science
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• v.23 no.10
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• pp.1192-1198
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• 2013

To improve yeast strains for bioethanol production, yeasts with ethanol tolerance, thermotolerance, and ${\beta}$-1,3-glucanase activity were bred using yeast genome shuffling. Saccharomyces cerevisiae $BY4742{\Delta}exg1$/pAInu-exgA, which has extracellular ${\beta}$-1,3-glucanase activity, and the Aspergillus oryzae and S. cerevisiae YKY020 strains, which exhibit ethanol tolerance and thermotolerance, were fused by yeast protoplast fusion. Following cell fusion, four candidate cells (No. 3, 9, 11, and 12 strains) showing thermotolerance at $40^{\circ}C$ were selected, and their ethanol tolerance (7% ethanol concentration) and ${\beta}$-1,3-glucanase activity were subsequently analyzed. All the phenotypes of the two parent cells were simultaneously expressed in one (No. 11) of the four candidate cells, and this strain was called BYK-F11. The BYK-F11 fused cell showed enhanced cell growth, ethanol tolerance, ${\beta}$-1,3-glucanase activity, and ethanol productivity compared with the $BY4742{\Delta}exg1$/pAInu-exgA and YKY020 strains. The results prove that a new yeast strain with different characters and the same mating type can be easily bred by protoplast fusion of yeasts.

• Journal of Life Science
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• v.23 no.7
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• pp.863-868
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• 2013

The XylP gene, which encodes endoxylanase in Bacillus sp. HY-20, was subcloned, and two expression plasmids, pG-xylP and pGMF-xylP were constructed. These plasmids, which contain different signal sequences, XylP s.s and $MF{\alpha}_{opt}$ s.s, respectively, for the secretory expression of endoxylanase, were transformed into Saccharomyces cerevisiae SEY2102 and FY833, respectively. The recombinant endoxylanases were successfully expressed, with a total activity range of 23.7-70.1 unit/ml according to the expression system and host strain. The endoxylanase activity in SEY2102/pGMF-xylP reached a maximum of 88.1 unit/ml in baffled flask culture. Most of the recombinant endoxylanase was efficiently secreted in the extracellular fraction, and the $MF{\alpha}_{opt}$ s.s was more efficient for secreting endoxylanase in yeast than the XylP s.s. Therefore, the expression system developed in this study produces large extracellular amounts of endoxylanase using S. cerevisiae as the host strain, and it could be used in bioethanol production and industrial applications.

• Clean Technology
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• v.20 no.2
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• pp.103-107
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• 2014

Acrylic acid is a commodity chemical which is applicable for various industries such as polymer and textile industry. Currently, it has been produced by chemical synthesis from petroleum. However, due to the high price of petroleum and global $CO_2$ emission, renewable materials such as sugar are interesting alternative carbon sources for the biological production of acrylic acid. For an economic production of acrylic acid from renewable carbon sources, a cost effective separation process for acrylic acid should be needed. In this study, reactive extraction by TOA (tri-n-octylamine) was used for the recovery of acrylic acid from its aqueous solutions. The effects of polarity of diluents and concentration of TOA on extraction equilibrium were investigated. The extraction efficiency was proportional to concentration of TOA and polarity of diluents and its value was more than 95% in the case of sufficient concentration of TOA. From IR spectroscopy, it was concluded that the ratio of (1,1) acid-amine complex was increased and the ratio of acid dimer was decreased with concentration of TOA. Equilibrium model based on IR spectroscopy was well fitted with experimental data.

• Journal of the Korean Wood Science and Technology
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• v.42 no.3
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• pp.318-326
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• 2014

This study was conducted to investigate the potential of non-used forest biomass residues as raw materials for making wood pellets with additives such as wood tar and starch and to evaluate fuel characteristics of the pellets. Wood tar, a by-product provided from the carbonization process of wood, could be a suitable additive for wood pellet production due to its higher calorific value and lower hazardous heavy metals, such as cadmium and mercury, compared to woody biomass. When the wood tar (10 wt%) was added, the calorific value was increased from 4,630 kcal/kg (wood pellet without additive) to 4,800 kcal/kg (wood pellet with additive). With the increase of additive amount into wood pellet, the length and individual density of wood pellet increased. In addition, bulk density of the pellets was increased, whereas the fine content was decreased. Consequently the overall productivity of wood pellets was improved by adding 2 w% additives into wood pellets; the percentage of productivity increase was 5.9% and 4.9% for adding starch and wood tar, respectively.

• Journal of Plant Biotechnology
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• v.41 no.1
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• pp.10-18
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• 2014

Seed oils (triacylglycerols) of plants are used as a source of essential fatty acids and nutrition for human. In addition, triacylglycerols have been used as industrial raw materials and biofuels. Triacylglycerols are mainly accumulated in seeds by complicated biochemical pathways. Fatty acids are synthesized in the plastids and transported into the endoplasmic reticulum for synthesizing triacylglycerols. It is known for a long time that biosynthesis of triacylglycerols is performed by a de novo synthesis, the Kennedy pathway. However recent studies have revealed that phosphatidylcholine, a major component of cell membrane, plays a central role for triacylglycerols biosynthesis. Phosphatidylcholine is a key regulator determining the relative proportions of unsaturated fatty acids in seeds. It may be a major carrier for the fluxes of fatty acids from the plastid to the endoplasmic reticulum. This finding further suggests that studies of the functions of enzymes involved in the fluxes of fatty acids from phosphatidylcholine to triacylglycerols elucidate the specialized subdomains in the endoplasmic reticulum for triacylglycerols biosynthesis.

• Journal of Science and Technology Studies
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• v.19 no.3
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• pp.291-333
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• 2019

The regional innovation policies so far have been separated from the social problems facing the local communities. The regional innovation policies, regarding the region as the location of the business, have focused on the invigoration of business innovation activities. However, as the recent emergence of the new paradigm of innovation policy aiming the sustainability, 'transformative innovation policy,' has led to a search for regional innovation policies that begin with solving the local social problems. This research paper deals with regional innovation theory that starts from searching for solutions and system transformation for social problems such as climate crisis and energy problems. The objective is to present a new framework called 'transformative regional innovation policy' and to improve its content through case studies by combining the results of the transformative innovation policy and the regional innovation policy studies. In particular, the contribution of this paper is to analyze and discuss the concept of the transition platform, which aims to solve the local social problems, through the case studies of Gussing, Austria and Esbjerg, Denmark. Lastly, it discusses the derived implications of the cases applied in Korean society.