Current research on seed oil biosynthesis
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Kim, Hyun Uk
(Department of Agricultural Biotechnology, National Academy of Agricultural Science, Rural Development Administration)
Lee, Kyeong-Ryeol (Department of Agricultural Biotechnology, National Academy of Agricultural Science, Rural Development Administration) Kim, Eun Ha (Department of Agricultural Biotechnology, National Academy of Agricultural Science, Rural Development Administration) Jung, Su-Jin (Department of Agricultural Biotechnology, National Academy of Agricultural Science, Rural Development Administration) Roh, Kyung Hee (Department of Agricultural Biotechnology, National Academy of Agricultural Science, Rural Development Administration) Kang, Han Chul (Department of Agricultural Biotechnology, National Academy of Agricultural Science, Rural Development Administration) Kim, Jong-Bum (Department of Agricultural Biotechnology, National Academy of Agricultural Science, Rural Development Administration) |
1 | Bourrellier ABF, Valot B, Guillot A, Ambard-Bretteville F, Vidal J, Hodges M (2010) Chloroplast acetyl-CoA carboxylase activity is 2-oxoglutarate-regulated by interaction of PII with the biotin carboxyl carrier subunit. Proc Natl Acad Sci USA 107:502-507 DOI ScienceOn |
2 | Burgal J, Shockey J, Lu CF, Dyer J, Larson T, Graham I, Browse J (2008) Metabolic engineering of hydroxy fatty acid production in plants: RcDGAT2 drives dramatic increases in ricinoleate levels in seed oil. Plant Biotechnol J 6:819-831 DOI ScienceOn |
3 | Cahoon EB, Ohlrogge JB (1994) Apparent role of phosphatidylcholine in the metabolism of petroselinic acid in developing umbelliferae endosperm. Plant Physiol 104:845-855 DOI |
4 | Cernac A, Benning C (2004) WRINKLED1 encodes an AP2/EREB domain protein involved in the control of storage compound biosynthesis in Arabidopsis. Plant J 40:575-585 DOI ScienceOn |
5 | Chapman KD, Dyer JM, Mullen RT (2012) Biogenesis and functions of lipid droplets in plants: thematic review series: lipid droplet synthesis and metabolism: from yeast to man. J Lipid Res 53:215-226 DOI |
6 | Andersson MX, Goksor M, Sandelius AS (2007) Optical manipulation reveals strong attracting forces at membrane contact sites between endoplasmic reticulum and chloroplasts. J Biol Chem 282:1170-1174 DOI ScienceOn |
7 | Andre C, Haslam RP, Shanklin J (2012) Feedback regulation of plastidic acetyl-CoA carboxylase by 18:1-acyl carrier protein in Brassica napus. Proc Natl Acad Sci USA 109:10107-10112 DOI |
8 | Banas W, Garcia AS, Banas A, Stymne S (2013) Activities of acyl-CoA:diacylglycerol acyltransferase (DGAT) andphospholipid: diacylglycerol acyltransferase (PDAT) in microsomal preparations of developing sunflower and safflower seeds. Planta 237:1627-1636 DOI ScienceOn |
9 | Barron EJ, Stumpf PK (1962) Fat metabolism in higher plants, XIX. The biosynthesis of triglycerides by avocado-mesocarp enzymes. Biochim Biophys Acta 60:329-337 DOI |
10 | Bates PD, Browse J (2011) The pathway of triacylglycerol synthesis through phosphatidylcholine in Arabidopsis produces a bottleneck for the accumulation of unusual fatty acids in transgenic seeds. Plant J 68:387-399 DOI ScienceOn |
11 | Yang W, Simpson JP, Li-Beisson Y, Beisson F, Pollard M, Ohlrogge JB (2012) A land-plant-specific glycerol-3-phosphate acyltransferase family in Arabidopsis: substrate specificity, sn-2 preference, and evolution. Plant Physiol 160:638-652 DOI |
12 | Wendel AA, Lewin TM, Coleman RA (2009) Glycerol-3-phosphate acyltransferases: rate limiting enzymes of triacylglycerol biosynthesis. Biochim Biophys Acta: Mol Cell Biol Lipids 1791:501-506 DOI |
13 | Weselake RJ, Taylor DC, Rahman MH, Shah S, Laroche A, McVetty PBE, Harwood JL (2009) Increasing the flow of carbon into seed oil. Biotechnol Adv 27:866-878 DOI |
14 | Xu J, Carlsson A, Francis T, Zhang M, Hoffmann T, Giblin M, Taylor D (2012) Triacylglycerol synthesis by PDAT1 in the absence of DGAT1 activity is dependent on re-acylation of LPC by LPCAT2. BMC Plant Biol, 12:4 DOI |
15 | Zhang M, Fan J, Taylor DC, Ohlrogge JB (2009) DGAT1 and PDAT1 Acyltransferases have overlapping functions in Arabidopsis triacylglycerol biosynthesis and are essential for normal pollen and seed development. Plant Cell 21:3885-3901 DOI |
16 | Zhao L, Katavic V, Li F, Haughn GW, Kunst L (2010) Insertional mutant analysis reveals that long-chain acyl-CoA synthetase 1 (LACS1), but not LACS8, functionally overlaps with LACS9 in Arabidopsis seed oil biosynthesis. Plant J, 64:1048-1058 DOI |
17 | Zou JT, Wei YD, Jako C, Kumar A, Selvaraj G, Taylor DC (1999) The Arabidopsis thaliana TAG1 mutant has a mutation in a diacylglycerol acyltransferase gene. Plant J 19:645-653 DOI ScienceOn |
18 | Tjellstrom H, Yang Z, Allen DK, Ohlrogge JB (2012) Rapid kinetic labeling of Arabidopsis cell suspension cultures: implications for models of lipid export from plastids. Plant Physiol 158:601-611 DOI |
19 | Turchetto-Zolet A, Maraschin F, de Morais G, Cagliari A, Andrade C, Margis-Pinheiro M, Margis R (2011) Evolutionary view of acyl-CoA diacylglycerol acyltransferase (DGAT), a key enzyme in neutral lipid biosynthesis. BMC Evol Biol 11:263 DOI |
20 | Taylor DC, Zhang Y, Kumar A, Francis T, Giblin EM, Barton DL, Ferrie JR, Laroche A, Shah S, Zhu W et al. (2009) Molecular modification of triacylglycerol accumulation by overexpression of DGAT1 to produce canola with increased seed oil content under field conditions. Botany Botanique 87:533-543 DOI |
21 | To A, Joube' s J, Barthole G, Le' cureuil A, Scagnelli A, Jasinski S, Lepiniec L, Baud S (2012) WRINKLED transcription factors orchestrate tissue-specific regulation of fatty acid biosynthesis in Arabidopsis. Plant Cell 24:5007-5023 DOI |
22 | van Erp H, Bates PD, Burgal J, Shockey J, Browse J (2011) Castor phospholipid: diacylglycerol acyltransferase facilitates efficient metabolism of hydroxy fatty acids in transgenic Arabidopsis. Plant Physiol 155:683-693 DOI |
23 | Wang L, Shen W, Kazachkov M, Chen G, Chen Q, Carlsson AS, Stymne S, Weselake RJ, Zou J (2012) Metabolic interactions between the lands cycle and the Kennedy pathway of glycerolipid synthesis in Arabidopsis developing seeds. Plant Cell 24:4652-5669 DOI |
24 | Weiss SB, Kennedy EP, Kiyasu JY (1960) Enzymatic synthesis of triglycerides. J Biol Chem 235:40-44 |
25 | Slack CR, Roughan PG, Browse JA, Gardiner SE (1985) Some properties of cholinephosphotransferase from developing safflower cotyledons. Biochim Biophys Acta 833:438-448 DOI |
26 | Shockey J, Gidda S, Chapital D, Kuan J, Dhanoa P, Bland J, Rothstein S, Mullen R, Dyer J (2006) Tung tree DGAT1 and DGAT2 have nonredundant functions in triacylglycerol biosynthesis and are localized to different subdomains of the endoplasmic reticulum. Plant Cell 18:2294-2313 DOI |
27 | Shen B, Allen WB, Zheng PZ, Li CJ, Glassman K, Ranch J, Nubel D, Tarczynski MC (2010) Expression of ZmLEC1 and ZmWRI1 increases seed oil production in maize. Plant Physiol 153:980-987 DOI |
28 | Slack CR, Campbell LC, Browse JA, Roughan PG (1983) Some evidence for the reversibility of the cholinephosphotransferase catalysed reaction in developing linseed cotyledons in vivo. Biochim Biophys Acta 754:10-20 DOI ScienceOn |
29 | Snyder CL, Yurchenko OP, Siloto RMP, Chen X, Liu Q, Mietkiewska E, Weselake RJ (2009) Acyltransferase action in the modification of seed oil biosynthesis. New Biotechnol 26:11-16 DOI |
30 | Sperling P, Linscheid M, Stocker S, Muhlbach HP, Heinz E (1993) In vivo desaturation of cis-delta-9-monounsaturated to cis-delta-9, 12-diunsaturated alkenylether glycerolipids. J Biol Chem 268:26935-26940 |
31 | Stalberg K, Stahl U, Stymne S, Ohlrogge J (2009) Characterization of two Arabidopsis thaliana acyltransferases with preference for lysophosphatidylethanolamine. BMC Plant Biol 9:60 DOI |
32 | Stymne S, Stobart AK (1984) Evidence for the reversibility of the acyl coA-lysophosphatidylcholine acyltransferase in microsomal preparations from developing safflower (Carthamus-tinctorius L.) cotyledons and rat-liver. Biochem J 223:305-314 DOI |
33 | Millar AA, Smith MA, Kunst L (2000) All fatty acids are not equal: discrimination in plant membrane lipids. Trends Plant Sci 5:95-101 DOI ScienceOn |
34 | Nikolau BJ, Ohlrogge JB, Wurtele ES (2003) Plant biotin-containing carboxylases. Arch Biochem Biophys 414:211-222 DOI |
35 | Maisonneuve S, Bessoule J-J, Lessire R, Delseny M, Roscoe TJ (2010) Expression of rapeseed microsomal lysophosphatidic acid acyltransferase isozymes enhances seed oil content in Arabidopsis. Plant Physiol 152:670-684 DOI |
36 | Mhaske V, Beldjilali K, Ohlrogge J, Pollard M (2005) Isolation and characterization of an Arabidopsis thaliana knockout line for phospholipid: diacylglycerol transacylase gene (At5g13640). Plant Physiol Biochem 43:413-417 DOI ScienceOn |
37 | Rani SH, Krishna THA, Saha S, Negi AS, Rajasekharan R (2010) Defective in Cuticular Ridges (DCR) of Arabidopsis thaliana, a gene associated with surface cutin formation encodes a soluble diacylglycerol acyltransferase. J Biol Chem 285:38337-3834. DOI |
38 | Saha S, Enugutti B, Rajakumari S, Rajasekharan R (2006) Cytosolic triacylglycerol biosynthetic pathway in oilseeds. Molecular cloning and expression of peanut cytosolic diacylglycerol acyltransferase. Plant Physiol 141:1533-1543 DOI ScienceOn |
39 | Schnurr JA, Shockey JM, de Boer GJ, Browse JA (2002) Fatty acid export from the chloroplast. Molecular characterization of a major plastidial acyl-coenzyme A synthetase from Arabidopsis. Plant Physiol 129:1700-1709 DOI |
40 | Schultz DJ, Ohlrogge JB (2000) Biosynthesis of triacylglycerol in Thunbergia alata: additional evidence for involvement of phosphatidylcholine in unusual monoenoic oil production. Plant Physiol Biochem 38:169-175 DOI |
41 | Lippold F, vom Dorp K, Abraham M, Holzl G, Wewer V, Yilmaz JL, Lager I, Montandon C, Besagni C, Kessler F et al. (2012) Fatty acid phytyl ester synthesis in chloroplasts of Arabidopsis. Plant Cell 24:2001-2014 DOI |
42 | Lardizabal K, Effertz R, Levering C, Mai J, Pedroso MC, Jury T, Aasen E, Gruys K, Bennett K (2008) Expression of Umbelopsis ramanniana DGAT2A in seed increases oil in soybean. Plant Physiol 148:89-96 DOI |
43 | Li-Beisson Y, Shorrosh B, Beisson F, Andersson M, Arondel V, Bates P, Baud S, Bird D, DeBono A, Durrett T et al. (2013) Acyl lipid metabolism. The Arabidopsis Book. e0161 |
44 | Kroon JTM, Wei W, Simon WJ, Slabas AR (2006) Identification and functional expression of a type 2 acyl-CoA:diacylglycerol acyltransferase (DGAT2) in developing castor bean seeds which has high homology to the major triglyceride biosynthetic enzyme of fungi and animals. Phytochemistry 67:2541-2549 DOI ScienceOn |
45 | Liu Q, Siloto RMP, Lehner R, Stone SJ, Weselake RJ (2012) Acyl-CoA: diacylglycerol acyltransferase: molecular biology, biochemistry and biotechnology. Prog Lipid Res 51:350-377 DOI |
46 | Lu C, Xin Z, Ren Z, Miquel M, Browse J (2009) An enzyme regulating triacylglycerol composition is encoded by the ROD1 gene of Arabidopsis. Proc Natl Acad Sci USA 106:18837-18842 DOI |
47 | Lung SC, Weselake RJ (2006) Diacylglycerol acyltransferase: a key mediator of plant triacylglycerol synthesis. Lipids 41:1073-1088 DOI |
48 | Hu Z, Ren Z, Lu C (2012) The phosphatidylcholine diacylglycerol cholinephosphotransferase is required for efficient hydroxyl fatty acid accumulation in transgenic Arabidopsis. Plant Physiol 158:1944-1954 DOI |
49 | Herna'ndez ML, Whitehead L, He Z, Gazda V, Gilday A, Kozhevnikova E, Vaistij FE, Larson TR, Graham IA (2012) A cytosolic acyltransferase contributes to triacylglycerol synthesis in sucrose-rescued Arabidopsis seed oil catabolism mutants. Plant Physiol 160:215-225 DOI |
50 |
Hay J, Schwender J (2011) Computational analysis of storage synthesis in developing Brassica napus L. (oilseed rape) embryos: flux variability analysis in relation to |
51 | Kennedy EP (1961) Biosynthesis of complex lipids. Fed Proc 20:934-940 |
52 | Kim HU, Lee K-R, Go YS, Jung JH, Suh M-C, Kim JB (2011) Endoplasmic reticulum-located PDAT1-2 from castor bean enhances hydroxy fatty acid accumulation in transgenic plants. Plant Cell Physiol 52:983-993 DOI ScienceOn |
53 | Kim S, Yamaoka Y, Ono H, Kim H, Shim D, Maeshima M, Martinoia E, Cahoon EB, Nishida I, Lee Y (2013) AtABCA9 transporter supplies fatty acids for lipid synthesis to the endoplasmic reticulum. Proc Natl Acad Sci USA 110:773-778 DOI |
54 | Koo AJK, Ohlrogge JB, Pollard M (2004) On the export of fatty acids from the chloroplast. J Biol Chem 279:16101-16110 DOI ScienceOn |
55 | Dahlqvist A, Stahl U, Lenman M, Banas A, Lee M, Sandager L, Ronne H, Stymne H (2000) Phospholipid: diacylglycerol acyltransferase: An enzyme that catalyzes the acyl-CoA independent formation of triacylglycerol in yeast and plants. Proc Natl Acad Sci USA 97:6487-6492 DOI ScienceOn |
56 | Chapman KD, Ohlrogge JB (2012) Compartmentation of triacylglycerol accumulation in plants. J Biol Chem 287:2288-2294 DOI |
57 | Gidda SK, Shockey JM, Rothstein SJ, Dyer JM, Mullen RT (2009) Arabidopsis thaliana GPAT8 and GPAT9 are localized to the ER and possess distinct ER retrieval signals: functional divergence of the dilysine ER retrieval motif in plant cells. Plant Physiol Biochem 47:867-879 DOI ScienceOn |
58 | Chen G, Snyder CL, Greer MS, Weselake RJ (2011) Biology and biochemistry of plant phospholipases. Crit Rev Plant Sci 30:239-258 DOI ScienceOn |
59 | Durrett T, McClosky D, Tumaney A, Elzinga D, Ohlrogge J, Pollard M (2010) A distinct DGAT with sn-3 acetyltransferase activity that synthesizes unusual, reduced-viscosity oils in Euonymus and transgenic seeds. Proc Natl Acad Sci USA 107:9464-9469 DOI ScienceOn |
60 | Eastmond PJ, Quettier A-L, Kroon JTM, Craddock C, Adams N, Slabas AR (2010) PHOSPHATIDIC ACID PHOSPHOHYDROLASE1 and 2 regulate phospholipid synthesis at the endoplasmic reticulum in Arabidopsis. Plant Cell 22:2796-2811 DOI ScienceOn |
61 | Bates PD, Ohlrogge JB, Pollard M (2007) Incorporation of newly synthesized fatty acids into cytosolic glycerolipids in pea leaves occurs via acyl editing. J Biol Chem 282:31206-31216 DOI ScienceOn |
62 | Bates PD, Browse J (2012) The significance of different diacylgycerol synthesis pathways on plant oil composition and bioengineering. Front Plant Sci 3:147 |
63 | Bates PD, Durrett TP, Ohlrogge JB, Pollard M (2009) Analysis of Acyl fluxes through multiple pathways of triacylglycerol synthesis in developing soybean embryos. Plant Physiol 150:55-72 DOI ScienceOn |
64 | Bates PD, Fatihi A, Snapp AR, Carlsson AS, Browse J, Lu C (2012) Acyl editing and headgroup exchange are the major mechanisms that direct polyunsaturated fatty acid flux into triacylglycerols. Plant Physiol 160:1530-1539 DOI |
65 | Gidda SK, Shockey JM, Falcone M, Kim PK, Rothstein SJ, Andrews DW, Dyer JM, Mullen RT (2011) Hydrophobic-domain-dependent protein-protein interactions mediate the localization of GPAT enzymes to ER subdomains. Traffic 12:452-472 DOI ScienceOn |
66 | Mora G, Scharnewski M, Fulda M (2012) Neutral lipid metabolism influences phospholipid synthesis and deacylation in Saccharomyces cerevisiae. PLOS One 7:e49269. DOI |
67 | Katavic V, Reed DW, Taylor DC, Giblin EM, Barton DL, Zou JT, Mackenzie SL, Covello PS, Kunst L (1995) Alteration of seed fatty-acid composition by an ethyl methanesulfonate-induced mutation in Arabidopsis thaliana affecting diacylglycerol acyltransferase activity. Plant Physiol 108:399-409 DOI ScienceOn |
68 | Chen X, Snyder CL, Truksa M, Shah S, Weselake RJ (2011) sn-Glycerol-3-phosphate acyltransferases in plants. Plant Signal Behav 6:1695-1699 DOI |
69 | Vandeloo FJ, Broun P, Turner S, Somerville C (1995) An oleate 12-hydroxylase from Ricinus communis L. is a fatty acyl desaturase homolog. Proc Natl Acad Sci USA 92:6743-6747 DOI ScienceOn |
70 | Li R, Yu K, Hatanaka T, Hildebrand DF (2010) Vernonia DGATs increase accumulation of epoxy fatty acids in oil. Plant Biotechnol J 8:184-195 DOI |
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