• Journal of the Society of Cosmetic Scientists of Korea
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• v.47 no.2
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• pp.147-153
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• 2021

Collagen hydrolysate (CH) is known to prevent skin aging by stimulating skin dermal fibroblasts to promote synthesis of extracellular matrix such as collagen and elastin. Recently, among the various factors that cause skin aging, advanced glycation end products (AGEs) have received particular attention. However, the effect of CH on AGE accumulation has not been studied. Since CH could affect AGE accumulation by promoting production of skin structural proteins, clinical trial was performed using low molecule collagen peptide (LMCP), which were CH containing 25% tripeptide and 4% Gly-Pro-Hyp. Skin autofluorescence (SAF) values were measured using an AGE reader to evaluate accumulation of AGE in skin. As a result of applying 0.5% and 1.0% LMCP solutions to the subject's forearm for 8 weeks, the SAF value at the test site significantly decreased compared to the control site. Additionally, in vitro test was performed using CCD-986sk to evaluate the promotion of collagen synthesis in skin fibroblasts by LMCP. As a result, 800 ㎍/mL of LMCP significantly increased synthesis of human pro-collagen Iα1 (COL1A1) in CCD-986sk. Through this study, we have confirmed that tropical LMCP applications can promote collagen synthesis to help anti-glycation effects, suggesting that LMCP has potential as an anti-aging cosmetic material.

• Korean Journal of Pharmacognosy
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• v.37 no.4 s.147
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• pp.307-313
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• 2006

Reactive oxygen species (ROS) and formation of advanced glycation end products (AGEs) play an important role in the pathogenesis of diabetic nephropathy by hyperglycemia. To find natural agents improving diabetic nephropathy, 63 natural resources which used to the treatment of diabetes mellitus in a folk remedy were investigated with an in vitro system employing radical scavenging activity and inhibitory activity of AGEs formation. In results, the extracts of Aspalathus linearis, Rubus coreanus, Rosa rugosa, and Epimedium koreanum significantly inhibited the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical with $IC_{50}$ values less than $10{\mu}g/ml$. The extracts of Zea mays, Cucurbita moschata, Cudrania tricuspidata, and Aspalathus linearis effectively reduced the formation of AGEs compared with the positive control $N-acetyl-_L-cystenine$ (NAC) and aminoguanidine (AG). In addition, the extracts of Aspalathus linearis, Commelina communis, Cornus officinalis, and Lespodeza cuneata showed the all inhibitory activity against DPPH radical and AGEs formation. Also, these resources definitely showed the radical scavenging activity against peroxynitrite $(ONOO^-)$ and hydroxyl radical $({\cdot}OH)$ relating to high glucose-induced ROS production. Thus, these results suggest that some natural resources may regulate the initiation and progression of diabetic nephropathy through inhibition of ROS production and AGEs formation.

• Korean Journal of Pharmacognosy
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• v.46 no.3
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• pp.260-267
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• 2015

Advanced glycation end products (AGEs) have been postulated to play a central role in the development of diabetic complications. A variety of different agents that inhibit AGEs have been under investigation. In this study, 111 herbal medicines from China have been investigated with an in vitro evaluation system using AGEs formation inhibitory activity. Of these, 9 herbal medicines (IC₅₀: <5 μg/ml) were found to have significant AGEs formation inhibitory activity. Particularly, herbal medicines Barleria cristata (leaves), Calotropis gigantea (stems), Ardisia virens (leaves), Dalbergia yunnanensis (leaves) Pittosporum runcatum (leaves), Ardisia japonica (leaves), Rhododendron racemosum (twigs), Oxyria sinensiss (aerial parts), Pyrrosia calvata (whole plants), showed more potent inhibitory activity (approximately 15-40 fold) than the positive control aminoguanidine (IC₅₀: 76.47 μg/ml).

• Korean Journal of Pharmacognosy
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• v.46 no.3
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• pp.250-259
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• 2015

Advanced glycation end products (AGEs) have been implicated in diabetic complications. In this study, the inhibitory effect on AGEs formation of 156 Korean herbal medicines has been evaluated. Among them, 15 Korean herbal medicines were showed to have significant effect (IC₅₀: <10 μg/ml) compared to positive reference, aminoguandine (IC₅₀: 76.47±4.81 μg/ml). Especially, four herbal medicines, Alnus firma (leaves, IC₅₀: 3.25±0.10 μg/ml), Juncus decipiens (whole plants, IC₅₀: 4.30±0.44 μg/ml), Smilax china (stems, IC₅₀: 3.55±0.21 μg/ml), and Vicia amoena (Aerial parts, IC₅₀: 4.25±0.06 μg/ml) showed more potent inhibitory activity approximately 8-24 fold) than the positive control aminoguanidine.

• Korean Journal of Pharmacognosy
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• v.46 no.3
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• pp.268-278
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• 2015

In this study, 80 herbal medicines from Vietnam have been investigated with an in vitro evaluation system using advanced glycation end products (AGEs) formation inhibitory activity. Of these, 10 herbal medicines (IC₅₀: <5 μg/ml) were found to have significant AGEs formation inhibitory activity. Particularly, herbal medicines Strobilanthes pateriformis (aerial parts), Rhodamnia dumetorum (twigs), Glochidion rubrum (twigs), Dipterocarpus obtusifolius (twigs), Bombax ceiba (twigs), Amesiodendron chinense (twigs), Bauhinia coccinea (twigs), Lithocarpus laouanensis (twigs), Bauhinia bracteata (twigs) and Connarus paniculatus (twigs), showed more potent inhibitory activity (approximately 16-31 fold) than the positive control aminoguanidine (IC₅₀: 76.47 μg/ml).

• Korean Journal of Pharmacognosy
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• v.40 no.4
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• pp.388-393
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• 2009

Advanced glycation end products (AGEs) have been implicated in the development of diabetic complications. The AGEs inhibitors or cross-link breakers attenuate various functional and structural manifestations of diabetic complications. In this study, 64 herbal medicines from China and Vietnam have been investigated with an in vitro evaluation system using AGEs inhibitory activity. Of these, eight herbal medicines ($IC_{50}$<50 ${\mu}g$/ml) were found to have strong AGEs inhibitory activity compared with aminoguanidine (14 days, $IC_{50}$=75.98 ${\mu}g$/ml; 28 days, $IC_{50}$=88.27 ${\mu}g$/ml). Particularly, four herbal medicines, Buddleja officinalis (whole plant), Syzygium cuminii (leaf), Eugenia caryophyllate (seed), and Paeonia suffruticosa (root) showed more potent inhibitory activity (approximately 5-6 fold) than the positive control aminoguanidine.

• Korean Journal of Pharmacognosy
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• v.33 no.4 s.131
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• pp.308-311
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• 2002

Advanced glycation end products(AGEs) are largly involved in the pathogenesis of diabetic nephropathy. It is obvious that inhibition of AGEs formation is important in preventing the occurrence and progression of diabetic nephropathy. In diabetes, this reaction is greatly accerated and is important in the pathogenesis of diabetic complications, especially diabetic nephropathy. Therefore, to seek possible AGEs inhibitors in herbal medicines, unprocessed - and processed Magnolia Bark were examined in vitro as basic data for aniaml experiment. The content of magnolol in unprocessed Magnolia Bark was $0.796{\pm}0.072%$, and after processing was decreased to $0.586{\pm}0.101%(p<0.01)$. The content of AGEs was measured by their intrinsic fluorescence. The $IC_{50}({\mu}g/ml)$ values of aminoguanidine, unprocessed- and procesled Magnolia Bark are $38.845{\pm}8.36{\mu}g/ml$, $54.264{\pm}3.153{\mu}g/ml$ and $27.882{\pm}1.836{\mu}g/ml$, respectively. This result means that prcessed Magnolia Bark was more effective than aminoguanidine, as positive control.

• Korean Journal of Pharmacognosy
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• v.39 no.3
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• pp.223-227
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• 2008

Advanced glycation end products (AGEs) contribute to the progression of micro and macrovsacular complication of diabetes and therefore present a promising target for therapeutic agents. In this study, 40 Korean herbal medicines have been investigated with an in vitro evaluation system using AGEs inhibitory activity. Of these, 21 herbal medicines $(IC_{50}<50{\mu}g/ml)$ exhibited an inhibitory activity against AGEs formation compared with anminoguanidine $(IC_{50}=72.12{\mu}g/ml)$. Particularly, 7 herbal medicines, Actinidia arguta (root and stem), Crataegus pinnatifida (twig), Camellia japonica (whole), Kalopanax pictus (bark), Lagerstroemia indica (leaf-stem), Reynoutria sachalinensis (root) showed more potent inhibitory activity (approximately 3-10 fold) than the positive control aminoguanidine.

• Korean Journal of Environmental Biology
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• v.28 no.1
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• pp.8-13
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• 2010

This study aimed the role of gold nanoparticles (AuNP) in advanced glycation end-products (AGEs) induced migration and invasion in bovine retinal endothelial cells (BRECs). BRECs were isolated from the retina. Cell viability was confirmed by the MTT assay. In vitro wound migration assay was performed to investigate the migration of BRECs. In vitro tube formation was measured by on-gel system. Apoptosis induced by AuNP was confirmed by caspase-3 assay. AGE-bovine serum albumin (BSA) demonstrated increase of cell migration and proliferation in BRECs. In addition, AuNP regardless of the existence of AGE-BSA suppressed proliferation, migration, and angiogenesis. AuNP suppressed AGE-BSA induced migration and invasion, and induced apoptosis through caspase-3. As a results, AuNP have a potential anti-angiogenic effect for AGE-induced angiogenesis in vitro and offer possibility for the treatment of diabetic retinopathy.

• Korean Journal of Pharmacognosy
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• v.42 no.1
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• pp.46-53
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• 2011

Advanced glycation end products (AGEs) have been postulated to play a central role in the development of diabetic complications. A variety of different agents that inhibit AGEs have been under investigation. In this study, 66 herbal medicines from China have been investigated with an in vitro evaluation system using AGEs formation inhibitory activity. Of these, 31 herbal medicines ($IC_{50}$ < $50\;{\mu}g/ml$) were found to have significant AGEs formation inhibitory activity. Particularly, 5 herbal medicines, Camptotheca acuminata (branches and leaves), Quercus franchetii (branches), Camellia pitardii (leaves, branches, and fruits), Antidesma bunius (whole plants), and Loranthus parasiticus (whole plants) showed more potent inhibitory activity (approximately 6-20 fold) than the positive control aminoguanidine ($IC_{50}=52.96\;{\mu}g/ml$).