• FLOWER RESEARCH JOURNAL
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• v.18 no.4
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• pp.271-277
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• 2010

The study is aimed to obtain the basic data for developing new variations of wild spring orchid. The results was investigated the capsules' formational characteristics and the germination ratio after having been self-pollinated by dividing the flowering period into the 5 stages into budding time, semi-flowering, full-flowering, 10 days after flowering, and 20 days after flowering. The fruit setting ratio was the highest as 100% in the plant which had been pollinated 20 days after the flowering, while the weight of the capsule was heaviest in the orchid which had been pollinated in semi-flowering period. As the result of investigating the germination ratio by dividing the period into 5 stages, it was the highest in the plant which had been pollinated during the semi-flowering period, and in the result of investigating the germination ratio by dividing the seeds harvesting days into the 3 stages, such as, 150 days, 165 days and 180 days after the pollination, it was highest as 5% in the orchid whose seeds had been harvested 150 days after the pollination. In the result of examining the germination ratio of the seeds treated with NaOCl, the those treated with 2% of NaOCl showed the highest as 67% in the germination ratio.

• Korean Journal of Plant Taxonomy
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• v.35 no.1
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• pp.47-55
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• 2005

During the course of the field trip to Mt. Baekdu and Manchurian region especially along the border of North Korea, young flower buds were collected and fixed to analyze the meiotic configuration of chromosomes and to discuss the cytotaxonomic characteristics of these areas. A total of 29 taxa with 31 populations of vascular plants were analyzed chromosomally. Among them, 26 taxa with 28 populations were collected from Mt. Baekdu which include Papaver radicata var. pseudoradicata, Bupleurum euphorbioides, Oplopanax elatus, Silene jenisseensis, and Gentiana jamesii and three taxa collected from Manchurian region which include Scrophularia buergeriana, Ostericum grosseserratum and Callistephus chinensis. The first report of chromosome numbers were provided here for those species of Artemisia lagocephala as 2n = 9II, Cacalia komaroviana as 2n = 30II, Chrysanthemum zawadskii var. alpinum as 2n = 27II and Rodiola rosea as 2n = 11II,

• Korean Journal of Plant Resources
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• v.17 no.3
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• pp.314-322
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• 2004

This research has been undertaken to increase availiability of native Camellia japonica leaf and flower in Korea as a edible-medicinal resource. Chemical compositions, antioxidant and antimicrobial activities in different parts of camellia were investigated. Crude protein contained the highest in young leaves(14.22%) but less than 10% in different parts. The contents of crude fat and crude ash were 60.48% in seeds and 5.16% in mature leaves, respectively. Methanol extract of young leaf, flower, and flower bud in camellia showed strong antioxidant activity compared with different parts. Also, antioxidant activity of these was higher than that of BHT, but weaker than that of VtC. Antioxidant activity of various parts in camellia were in order of young leaf ＆gt; flower bud ＆gt; flower ＆gt; mature leaf ＆gt; stem ＆gt; bark. Extracts of mature leaf and bark had a remarkable antibacterial activity(0 CFU/$m\ell$) on Bacillus subtilis. The young leaf extract displayed effective growth inhibition against B. subtilis, Candida albicans, and Trichosporon beigelii.

• Horticultural Science & Technology
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• v.31 no.3
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• pp.359-365
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• 2013

Chrysanthemum is one of the most popular ornamental plants worldwide. Recently, lots of new and novel chrysanthemum varieties have been developed using mutagenesis. However, there was no study for comparison of tissue culture condition among the mutant varieties derived from one original variety, until now. This study was conducted to compare the efficient regeneration condition of the two chrysanthemum mutant varieties, 'ARTI-purple' and 'ARTI-queen'. Two different flower parts (disk and ray florets) at the unopened and early blooming stages were used for comparison of regeneration condition on MS medium supplemented with combinations of three growth regulators (BA, NAA, and IAA). The highest regeneration rate was identified on the NAA and BA combination when the disk florets at unopened blooming stage are used. The best optimum combinations of growth regulators were identified as NAA $1.0mg{\cdot}L^{-1}$ and BA $0.5mg{\cdot}L^{-1}$ at 'ARTI-purple', which displayed 47.9% regeneration. However, regeneration of 'ARTI-queen' was the highest as 25.6% at NAA $2.0mg{\cdot}L^{-1}$ and BA $1.0mg{\cdot}L^{-1}$. There results indicate that there is a difference for the optimum regeneration condition between the mutant varieties derived from one original variety. These results will be useful for construction of efficient regeneration system of diverse chrysanthemum mutants developed by mutation breeding.

• Journal of Plant Biotechnology
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• v.39 no.3
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• pp.140-145
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• 2012

The flowering locus T (FT) gene, of which expression will be controlled at high temperature by heat shock promoter (it printed as to HSproFT), was introduced into spray-type chrysanthemum (Dendranthema grandiflorum (Ramat.) Kitamura) 2 cultivars ('Pink PangPang' and 'Pink Pride' by co-cultivation with Agrobacterium tumefaciens strain C58C1 harboring pCAMBIA2300 containing the HSproFT gene. After leaf segments of the 2 cultivars were infected with the A. tumafaciens with C58C1 as explants, shoots were regenerated from the explants cultured on the $1^{st}$ selection medium (MS basal salts + 1.0 mg/L BA, 0.5 mg/L IAA + 10 mg/L kanamycin + 0.7% plant agar, pH 5.8). The shoots were transferred into the $2^{nd}$ selection medium (MS basal salts + 1.0 mg/L BA, 0.5 mg/L IAA + 20 mg/L kanamycin + 0.7% plant agar, pH 5.8). One hundred seventeen plantlets from 'Pink PangPang' and 5 ones from 'Pink Pride' were confirmed as transformants by PCR analysis. Twenty six of the transformants and non-transformants were acclimatized and established well in a green house. Eights of 26 transgenic lines showed flower bud 1.7~10 days earlier than nontransgenic plants, and 24 of them flowered 1~6 days earlier than non- transgenic plants. The shape and color of flower of all HSproFT-transgenic lines were not different with those of non- transgenic plants.

• Korean journal of applied entomology
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• v.53 no.1
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• pp.65-71
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• 2014

Frankliniella occidentalis is attracted to flowers and is a major pest of chrysanthemums. Even when some chrysanthemum plants are not flowering, the ones that have already flowered attract F. occidentalis. Therefore, we investigated the efficacy of chrysanthemum as a trap plant that attract F. occidentalis by using an olfactometer. The numbers of F. occidentalis collected from the flowers of pink, wihte and yellow standard chrysanthemums on a tray with wet paper during the flowering period were 18.4, 56.6, and 52.6 respectively; the numbers of F. occidentalis collected from leaves were 7.8, 16.6, and 15.4 respectively. the numbers of F. occidentalis collected from the buds of pink, white and yellow standard chrysanthemums were 15.2, 45.8, and 41.6 respectively; the numbers of F. occidentalis collected from the leaves were 2, 8.8 and 3.4 respectively. In the Y-tube olfactometer test, the number of F. occidentalis attracted to the 2-way arms of the Y-tube was not significantly different for the yellow, red, violet and white flowers. In the four-choice olfactometer test, when the same visual cues and odor cues were provided, the frequency of F. occidentalis was higher in the yellow (10.7) flowers than in the red (1.3), violet (3.7) and white (2.0) flowers. When visual cues with disturbed odor cues, F. occidentalis preferred yellow (10.0) color over red (3.3), violet (1.3) and white (3.0) colors. When the same visual and odor cues, except for yellow visual cues, were provided, F. occidentalis preferred white (8.3) color over red (4.7), violet (4.7) and yellow (2.0) colors. Therefore, F. occidentalis were attracted to buds before the flowering of chrysanthemum plants and attracted to yellow flowers after the flowering.

• Journal of Plant Biotechnology
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• v.44 no.1
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• pp.27-34
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• 2017

Our aim was to find the effective duration and culture conditions for microspore culture in a genetically variant cabbage (Brassica oleraceae L. var. capitata). We discovered that the '$2{\times}NLN$ medium containing $AgNO_3$ 1 mg/l, sucrose 13%' was most efficient in producing the cabbage embryos. The number of induced embryos was higher in $F_2$ or $F_3$ progeny generation than the $F_1$ hybrid cultivar used as plant materials. Thus, we recommend microspore culturing using progeny plants of failed $F_1$ cultivar. The acquisition ratio of embryos and plants derived from microspore was higher in the early flowering period as compared to the late flowering period. When transplanted in the soil, 71.2% plants developed from the early flowering period, compared to 27.0% from the middle period and 1.8% plants from the late period. Thus, we recommend microspore culture using buds collected during the early flowering period.

• Korean Journal of Medicinal Crop Science
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• v.4 no.3
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• pp.231-235
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• 1996

The experiment was conducted to determine pollen morphology of Chinese peony and its germination. The results were obtained as following ; Pollen shpae was usually large ellipse and the pol­len germination rate of Eui-seong Peony line was 71% which was lower than that of other Peony lines. In a bud, the pollen began to be observed on $7{\sim}9days$ before flowering and as getting on for flowering time, normal pollen and germination rates were higher. The pollen germination and elongation beganat 30 minutes after incubation on artificial medium and were completed after $2{\sim}3\;hours$. As the storage time went on, the pollen life span was shortened. The germination rate was 51% at 8 days-storage of room temperature. The germination rate was 48% ad 43% at 95 day-storage in $0^{\circ}C$ and $-15^{\circ}C$, respectively.

• Research in Plant Disease
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• v.10 no.4
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• pp.290-296
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• 2004

Bacterial blossom blight is one of the most important diseases of kiwifruit (Actinidia deliciosa). The disease occurs during flowering in the late May and disease outbreaks associated with rainfall during the flowering season have resulted in a severe reduction in kiwifruit production. The causal organism isolated from diseased blossoms of kiwifruits was identified as Pseudomonas syringae pv, syringae based on the physiological and biochemical characteristics and pathogenicity test. Dead fruit stalks, dead pruned twigs, fallen leaves and soils mainly provided R syringae pv. syringae with overwintering places in the kiwifruit orchards, and the inocula also overwintered on buds, trunks, branches, and twigs on the kiwifruit trees. Among the overwintering places, the incula were detected in the highest frequencies from dead fruit stalks. The population density of P. syringae pv. syringae was speculated to be over $1{\times}10^4$cfu/ml for the bacterial infection, and the optimum temperature for the bacterial growth ranged 20 to $25^{\circ}C$. The highest population density of P. syringae pv. syringae on the overwintering places was detected in May and June when the daily average temperature coincided with the optimum temperature for bacterial growth of P. syringae pv. syringae.

• Research in Plant Disease
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• v.26 no.3
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• pp.144-158
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• 2020

Quantitative reverse transcription PCR is used for gene expression analysis as the accurate and sensitive method. To analyze quantification of gene expression changes in apple plants, 10 housekeeping genes (ACT, CKL, EF-1α, GAPDH, MDH, PDI, THFs, UBC, UBC10, and WD40) were evaluated for their stability of expression during infection by Apple stem grooving virus (ASGV) or in cold-stress apple plant buds. Five reference-gene validation programs were used to establish the order of the most stable genes for ASGV as CKL>THFs>GAPDH>ACT, and the least stable genes WD40CKL>UBC10, and the least stable genes were ACT