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http://dx.doi.org/10.5010/JPB.2012.39.3.140

FT-transgenic spray-type Chrysanthemum (Dendranthema grandiflorum Kitamura) showing early-flowering  

Lee, Su-Young (Floriculture Division, National Institute of Horticultural and Herbal Science)
Han, Bong-Hee (The Foundation of Agric. Tech. Commercialization and Transfer)
Hur, Eun-Joo (Floriculture Division, National Institute of Horticultural and Herbal Science)
Shin, Hak-Kee (Floriculture Division, National Institute of Horticultural and Herbal Science)
Lee, Il-Ha (Biological Sciences, Seoul National University)
Lee, Eun-Kyung (Floriculture Division, National Institute of Horticultural and Herbal Science)
Kim, Seung-Tae (Floriculture Division, National Institute of Horticultural and Herbal Science)
Kim, Won-Hee (Floriculture Division, National Institute of Horticultural and Herbal Science)
Kwon, O-Hyeon (Floriculture Division, National Institute of Horticultural and Herbal Science)
Publication Information
Journal of Plant Biotechnology / v.39, no.3, 2012 , pp. 140-145 More about this Journal
Abstract
The flowering locus T (FT) gene, of which expression will be controlled at high temperature by heat shock promoter (it printed as to HSproFT), was introduced into spray-type chrysanthemum (Dendranthema grandiflorum (Ramat.) Kitamura) 2 cultivars ('Pink PangPang' and 'Pink Pride' by co-cultivation with Agrobacterium tumefaciens strain C58C1 harboring pCAMBIA2300 containing the HSproFT gene. After leaf segments of the 2 cultivars were infected with the A. tumafaciens with C58C1 as explants, shoots were regenerated from the explants cultured on the $1^{st}$ selection medium (MS basal salts + 1.0 mg/L BA, 0.5 mg/L IAA + 10 mg/L kanamycin + 0.7% plant agar, pH 5.8). The shoots were transferred into the $2^{nd}$ selection medium (MS basal salts + 1.0 mg/L BA, 0.5 mg/L IAA + 20 mg/L kanamycin + 0.7% plant agar, pH 5.8). One hundred seventeen plantlets from 'Pink PangPang' and 5 ones from 'Pink Pride' were confirmed as transformants by PCR analysis. Twenty six of the transformants and non-transformants were acclimatized and established well in a green house. Eights of 26 transgenic lines showed flower bud 1.7~10 days earlier than nontransgenic plants, and 24 of them flowered 1~6 days earlier than non- transgenic plants. The shape and color of flower of all HSproFT-transgenic lines were not different with those of non- transgenic plants.
Keywords
Agrobacterium; leaf segment; kanamycin; PCR; selction;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
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