• Microbiology and Biotechnology Letters
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• v.18 no.5
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• pp.517-524
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• 1990

The solubilization and desolubilization of proteins in CTAB/hexanol/isooctane reverse micellar system were investigated for the selective separation of proteins. Several proteins were used, including bovine serum albumin (BSA), pepsin, trysin and ribonuclease-a. Most proteins could be solubilized into reverse micelles in the pH range above the isoelectric point of each protein, where the net charge of protein was opposite to that of surfactant. However BSA was solubilized above pH 10, which is serveral pH units above the pI 4.9. The kinds of anions in aqueous phase influenced on protein solubilization while no significant trend was observed with different cations, Protein solubilization decreased with increase of the ion size in the order of F -, C1-, Br- and I -. The size of CTAB micelles did not change significantly with increasing ionic strength, but the solubilization decreased. Protein desolubilization showedropposite behaviors to the solubilization. Several model mixtures such as pepsin/ trypsin, pepsin/ribonuclease-a and BSAlribonucleaee-a were successfully separated from each other without changing enzymatic activities.

• Journal of Life Science
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• v.14 no.3
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• pp.509-513
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• 2004

Inhibitory activities of 58 methanol extracts, which were prepared from different parts of 55 kinds of edible and medicinal plants, against human thrombin were evaluated. Analysis of the anti-coagulation activity determined by thrombin time enabled us to select 8 herb extracts as possible sources of anti-thrombosis agent. Further analysis of anti-thrombosis activity determined by activated partial thromboplastin time and fibrinolytic activity, and heat stability and inhibition against other proteolytic digestive enzymes provided new information that Pimpinella brachycarpa, Lysimachia clethroides, and Salix gracilistyla could be used as a potential anti-thrombosis agent. Our results suggest that edible and medicinal plants could be the potential source of thrombin inhibitor.

• Korean Journal of Child Studies
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• v.30 no.6
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• pp.209-221
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• 2009

The early childhood education in Korea has made a great progress during the last thirty years. However, understanding the early childhood education is a challenging task because of the diversity of the fields. Currently 38% of 3-5 year old children are enrolled in kindergarten. And several efforts to develop and distribute the standard curricula on a national level were underwent successfully. Researches are rapidly expanding in volume and the topics are becoming diverse. Focuses of researches moved from the area of cognitive development, science and mathematics in 80s, social and emotional aspects in 90s to language, curriculum and teacher education in 2000s. One of the emerging issues is the inclusion of kindergarten to public education system and free kindergarten education for young children. The second issues is more interdisciplinary policies are in need related to the low-birth rate in nation. The third one is about teacher training policy. The fourth one is related to the health, nutrition and safety of young children. And the fifth issue is moving educational system and policies that provide better future of young children while focusing on the children in low income families and children with absolute poverty.

• Research in Community and Public Health Nursing
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• v.23 no.2
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• pp.155-164
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• 2012

Purpose: The purpose of this study was to investigate Seoul teacher's satisfaction with and needs for health examination and health promotion programs. Methods: The participants were 3,186 teachers in Seoul. Data were collected through an on-line survey system from November 16 to December 7, 2008. Collected data were analyzed by descriptive statistics, t-test, ANOVA, and Scheffe's test using PASW 18.0 program. Results: Teacher's satisfaction with teacher health examination was low and needs for detailed tests of cancer and vocal cord disorders were high. Teacher's satisfaction with teacher health promotion programs was low and needs for stress management, exercise and vocal cord protection program were high. Conclusion: Vocal cord disorders should be included in teacher health examination and vocal cord protection program should be provided for teacher's health promotion. Stress management and exercise program should also be provided for teacher's health promotion.

• Research in Community and Public Health Nursing
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• v.31 no.1
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• pp.54-64
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• 2020

Purpose: This study aims to identify related factors of psychological well-being of migrant workers in Korea. Methods: The subjects were 138 migrant workers residing legally in K or B Cities. Data were collected from July 1 to August 31, 2018, using self-report structured questionnaires that were translated into English, Chinese, Vietnamese, and Korean, and were analyzed with descriptive statistics, t-test, ANOVA, Pearson's correlation coefficients and multiple regression. Results: The most important influencing factors on the psychological well-being of migrant workers were social support (β=.36, p<.001), followed by perceived health status (β=.25, p=.001), education (β=-.18, p=.015), and spouse support (β=.16, p=.032). The overall explanatory power was 28.9%. Conclusion: In order to improve the psychological well-being of migrant workers, it is required to develop a nursing approach that may promote the social support, health status, and spouse support, and consider the educational level of migrant workers.

• Journal of the Korean Geographical Society
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• v.38 no.5
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• pp.769-785
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• 2003

The regional distribution of the selling amount on the internet shopping is divided into urban areas and rural areas. In the urban areas, a large amount of selling and various commodity groups are purchased. In the rural areas, a small amount of selling simple clothing goods, home kitchen goods, and the others commodity groups are purchased. The regional distribution is considered dependent upon the size of population, the development degree of the education and service industry in the region, and the buying power of women in the 25 to 49 year group. This regional trend shows the difference of each population composition between urban and rural areas during the period of economic growth in our country affects internet shopping. Therefore consumer's limited purchase behavior is apparent in rural areas.

• Journal of Environmental Science International
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• v.11 no.6
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• pp.471-482
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• 2002

The purpose of this study is in identifying the attraction degree of Seonunsan Provincial Park by quantitative analysis. For this, landscape slide test was performed by questionaries. And multiple regression analysis and Factor analysis were performed far identifying the attraction degree of users. The attraction degree of natural resources was evaluated by questionaries about the landscape scene of 120 slides which was pictured at the chief visual corridor So it is considered that tourism route far attracting people into natural characteristic resources should be developed. It is identified once again that factors of $\boxdr$Naturalness$\boxul$,$\boxdr$Uniqueness$\boxul$and $\boxdr$Mysteriousness$\boxul$are important in natural landscape. And 16 explanation variables which explain the attraction degree of viewing landscape were classified as 4 factors. Those were defined as $\boxdr$Ability (FⅠ)$\boxul$, $\boxdr$Season harmony (FII)$\boxul$,$\boxdr$Simplicity (FⅢ)$\boxul$ and $\boxdr$Legibility(FⅣ)$\boxul$. It is identified that the variables of FⅠ, mysteriousness, is a representative image which expresses the characteristics of Seonunsan Provincial Park.

• Child Health Nursing Research
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• v.16 no.2
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• pp.102-111
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• 2010

Purpose: This study was conducted to identify high school students' knowledge and attitude about cancer, and to examine the relation between these variables. Methods: The participants were 811 students from 8 high schools in U city. The data were analyzed using Pearson correlation coefficients, ANOVA and Duncan test with SPSS/WIN 12.0. Results: The score for participants' knowledge about cancer was 18.88 out of a possible 30, and their score for attitude towards cancer was 34.74 points of a possible 50. There were significant differences in knowledge about cancer according to gender, religion, school grades and worries about cancer. The attitude towards cancer showed significant differences according to worries about cancer and family atmosphere. The health behaviors were also statistically affected by factors like whether one smoked or not, health condition and harmony of family life. There was a significant positive correlation between attitude towards cancer and health behavior for cancer prevention. Attitude towards cancer and health behavior for cancer prevention showed a positive correlation with knowledge about cancer. Conclusion: The results of this study indicate that the development of an appropriate attitude towards cancer by high school students should lead to the practice of health behavior to prevent cancer. This development could be enhanced with structured and on-going education about cancer.

• Journal of Families and Better Life
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• v.26 no.4
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• pp.149-162
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• 2008

The purpose of this study is to provide a meaningful thought to professionals and administrators of Korea working for Consumer General Education development at their Universities. Also, tips for organizing and meaning Consumer General Education Curriculum effectively are provided. Finally, the study suggests essential elements to improve qualities of Consumer General Education in Korea Universities. This study's method are content analysis through web site and survey. The subjects of this survey were 257 students. The data were collected by qusetionaires developed by researcher and analyzed by frequencies, correlation Analysis, One Way Anova, Duncan Test, Factor Analysis, Multiple Regression Analysis. The major findings were as follows: University students's satisfaction level about General Education was low and the needs level about Consumer General Education was high. And the education service quality showed factors to be administrative service element, professor service element, classroom environment element, communication element. And Multiple Regression Analysis on each of the education service quality factors had been significant effects for find out the differences in the degree of satisfaction.

• Journal of Korean society of Dental Hygiene
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• v.14 no.3
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• pp.399-406
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• 2014

Objectives : The purpose of the study is to investigate the oral health behaviors between students studying in Vancouver, Canada and college students in Daejeon, Korea. Methods : A self-reported questionnaire was filled out by 239 students in November, 2013. The questionnaire consisted of oral health behavior including tooth brushing methods between two countries. Chi-square test and t-test were analyzed. The analyses were conducted using R version 2.14.2(R Foundation for statistical Computing, Vienna, Austria). Results : The number of tooth brushing in Korean students was higher than those in Canada. Fifty nine percent of the Koreans brushed their teeth 3 times a day while 62.3% of students in Canada brushed their teeth 2 times a day(p<0.0001). Those who were taught on oral health education tended to brush the teeth more frequently that those who had not. Koreans and Japanese tended to brush teeth more frequently after breakfast than the Taiwanese(p=0.005). Koreans also brushed teeth after dinner more frequently than Japanese, Taiwanese, and other foreigners(p=0.012). Conclusions : This study indicated that Koreans have better oral health behaviors in comparison to students studying abroad.