• The Korean Journal of Mycology
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• v.38 no.1
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• pp.34-39
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• 2010

We have attempted to verify 30 strains of Hypsizigus marmoreus from various mushroom stocks in Korea using random amplified polymorphic DNA (RAPD) methodology. Chromosomal DNAs of them were extracted and subjected to PCR analyses with 3 random primers. Each PCR produced approximately 30 distinct PCR bands with the size from 200 bp to 3000 bp. A dendrogram was acquired using the unweighted pair-group method with arithmetic average (UPGMA) clustering methodology on the basis of the DNA band pattern. The analysis revealed that 30 strains of H. marmoreus were clustered into two distinct clusters. Cluster 1 contained 3 subgroups while the cluster 2 consisted of rather diverse strains. Interestingly, Hm3-10, a wild strain collected from Deog-Yu mountain, was not included in either clusters, indicative of uniqueness of this strain. We nextly attempted to develop strain-specific DNA markers to verify a specific strain. A unique band in the RAPD gel lane of Hm0-4 was extracted and its sequence was determined. PCR with a primer set from the determined sequence revealed that the primer set gave a 250 bp DNA band only for Hm0-4, indicating that this approach works well for the strain-specific identification of H. marmoreus.

• Research in Plant Disease
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• v.14 no.1
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• pp.71-75
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• 2008

An isolate of Peanut stunt virus (PSV), named as Tr-PSV, was isolated from white clover (Trifolium repens L) showing mosaic symptom. Tr-PSV systemically infected all plants tested in the Nicotiana spp. and induced local lesions on inoculated leaves of Chenopodium amaranticolor. However, Tr-PSV induced typical mosaic symptoms as ER-PSV on Vigna unguiculata 5 to 6 days after inoculation, while Fny-CMV used as a control virus of Cucumovirus produced local lesions on inoculated leaves. In dsRNA analysis, Tr-PSV consisted of four dsRNAs, but satellite RNA was not detected. The cDNA of coat protein gene of Tr-PSV was amplified by RT-PCR using a Cucumovirus-specific single pair primers that designed to amplify a DNA fragment of approximately 950 bp. By restriction mapping analysis using RFLP of the RT-PCR products and by serological properties of gel diffusion test, Tr-PSV belongs to a typical member of PSV subgroup I. This is the first report on the occurrence of PSV in white clover in Korea.

• Korean Journal of Organic Agriculture
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• v.22 no.4
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• pp.855-868
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• 2014

Synthetic agro-chemicals have been widely used to control diseases on paprika but these days negative attention has been increasing to use of them because of several adverse effects. This research was conducted to isolate and to characterize the antagonistic microorganism to control major paprika diseases, gray mold rot, fruit and stem rot, phytophthora blight, sclerotium rot, and wilt disease. Analysis of the fatty acid and analysis of the 16S rDNA gene sequence revealed that YKJ1 isolated in this research belongs to a group of Serratia marcescens. Specially, 16S rDNA gene sequence of YKJ1 showed 99% of sequence similarity with S. marcescens. Observation through the optical microscope revealed that YKJ1 suppressed the spore germination and the hyphal growth of pathogens. YKJ1 treatment on pathogens induced marked morphological changes like hyphal swelling and degradation of cell wall. In the case of phytophthora blight, the zoosporangium formation was restrained. S. marcescens found in this study call as S. marcescens-YKJ1 and it may be valuable as one of biological control agents against major diseases of paprika in the future even though it is require to be tested with more study on field test.

• Horticultural Science & Technology
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• v.31 no.5
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• pp.580-589
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• 2013

This study was carried out to evaluate the suitability of microsatellite markers for varietal identification and genetic relationship of 170 commercial pepper cultivars. The relationship between marker genotypes and 11 pepper cultivars with different morphological traits was also analyzed. Of the 302 pairs of microsatellite primers screened against 11 pepper cultivars, 24 pairs were highly polymorphic in terms of number of alleles. These markers were applied for the construction of DNA profile data base for 170 commercial pepper cultivars. A total of 164 polymorphic amplified fragments were obtained from 24 microsatellite primers. The average polymorphism information content was 0.673 ranging from 0.324 to 0.824. One hundred and sixty four microsatellite alleles were used to calculate Jaccard's distance coefficients using unweighted pair group method. A clustering group of varieties, based on the results of microsatellite analysis, were categorized into 3 major groups corresponding to morphological traits. The phenogram discriminated all varieties by markers genotypes. These microsatellite markers will be useful as a tool for protection of plant breeders' intellectual property rights through variety identification in distinctness, uniformity and stability test.

• Journal of KIISE:Computer Systems and Theory
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• v.26 no.7
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• pp.813-826
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• 1999

실시간 멀티미디어 응용의 등장으로 멀티캐스트와 QoS(Quality of Service) 지원이 필수적인 망 서비스로 부각되고 있다. 이에, ATM 기반의 인터넷에서 IP 멀티캐스트의 효율적인 처리를 위하여 MARS(Multicast Address Resolution Server)가 제안되었고, 기존의 최선 서비스 기반의 인터넷에서 QoS(Quality of Service)를 지원하기 위하여 RSVP(Resource Reservation Protocol)가 제안되었다. 본 논문에서는 ATM 망에서 QoS가 지원되는 IP 멀티캐스트 서비스를 제공하기 위하여 MARS 구조에서 RSVP를 지원하는 두 가지 방안을 제안하고, 시뮬레이션을 통하여 그 성능을 분석하였다. 제안하는 두 가지 방법은 각각 'RSVP 전 홉 노드 방식'과 'MARS 서버 방식'이라 명명하였다. RSVP 전 홉 노드 방식은 송신원으로부터 ATM 망으로 진입하는 노드와 수신원을 향하여 ATM 망을 진출하는 노드 간에 각각 일대일 양방향 VC를 설정하여 멀티캐스트 그룹에 속하는 수신원들이 보내는 자원 예약 메시지를 ATM 망에서 전송하는 방안이다. MARS 서버 방식은 ATM 망을 진출하는 노드와 MARS 서버간에 MARS 제어 메시지 교환을 위해 존재하는 ATM VC를 사용하여 RSVP의 자원 예약 메시지를 전송하고, MARS 서버가 RSVP 자원 예약 메시지를 처리하도록 그 기능을 확장함으로써 ATM 망에서 필요로 하는 제어 VC 수를 절약할 수 있는 방안이다. 시뮬레이션을 통하여, MARS 서버 방식은 ATM 제어 VC의 수를 절약할 수 있을 뿐 아니라 경우에 따라 RSVP 자원 예약 메시지 전달 지연을 줄일 수도 있음을 볼 수 있었다. 그러나, MARS 클러스터 내에 동시에 존재하는 RSVP 흐름이 많을 때에는 MARS 서버 방식의 경우 MARS 서버에서의 병목 현상으로 인해 성능이 저하될 수 있다.Abstract Emerging real time multimedia applications require multicast service with a QoS(Quality of Service) support. An overlay service architecture MARS(Multicast Address Resolution Server) is proposed to support IP multicast over an ATM network, and a resource reservation protocol RSVP is proposed to provide QoS support in the Internet which is originally based upon best effort service only. In this paper, we propose two schemes to support IP multicast service with QoS support over ATM networks: 'RSVP Previous Hop Node(RPHN) scheme' and 'MARS server based scheme'. In RPHN scheme, the RSVP reservation messages are transported via one-to-one ATM control VC from the egress nodes to the ingress nodes of the the multicast flow set up between each pair of nodes. The RSVP message processing occurs at the ingress nodes of the multicast flow. Whereas, in the MARS server based scheme, the RSVP reservation messages are transported via the MARS control VCs between the egress nodes and the MARS server. The RSVP message processing burden is imposed at MARS server in this scheme. For MARS server based scheme, no additional ATM VC is required for RSVP reservation message transmission, while the processing burden at the MARS server is high. Simulation results show that the MARS server based scheme, may accomplish RSVP reservation message delivery with smaller delay as well as saving of the number of ATM VCs. When the number of simultaneous RSVP flows in the MARS cluster is large, however, MARS based scheme may suffer performance degradation since MARS server becomes a performance bottleneck.

• Korean Journal of Plant Resources
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• v.32 no.4
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• pp.296-302
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• 2019

This study was accomplished to select breeding materials among the pear germplasm 6 species 43 accessions and check out the expression of scab incidence of inter specific hybrids of 5 crosses 609 seedlings for scab resistance breeding. Fruitlets scab incidence of P. pyrifolia, P. bretschneideri and P. ussuriensis were 59.5, 73.4 and 63.0% respectively, and also fruitlets scab incidence of interspecific hybrid cultivars between P. pyrifolia and P. bretschneideri were 77.7%. However, fruitlets scab incidence of P. communis and interspecific hybrid accessions between P. pyrifolia and P. communis were significantly less than 15%. As a result of the Duncan test, scab resistance of pear accessions were classified into two groups: P. communis and their hybrid were resistance and P. bretschneideri, P. pyrifolia, P. ussuriensis, and among their hybrid were susceptible. Also, it showed the same trend in the seedlings. Scab incidence of 'Seolwon' ${\times}$ 'Chuwhangbae' and 'Supergold' ${\times}$ 'Mantienhong' in P. pyrifolia seedlings showed more than 80%, while that of interspecific hybrid between P. pyrifolia and P. communis showed about 2%. It showed scab resistance of P. communis transferred to hybrid seedlings and expressed to be dominant in their seedlings. Namely, P. communis was good breeding materials for scab resistance.

• 한국노년학
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• v.38 no.4
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• pp.837-851
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• 2018

This study aimed to investigate the relationship between frailty and health care utilization in a cross-sectional design of a population-based sample of community-dwelling older adults. We used the data of 516 participants who dwell in Daejon, aged between 65 and 84 years old. Using K-frailty index, frailty status were measured and categorized as three groups: robust, prefrail, and frail. Logistic regression analysis was used to examine if frailty affects emergency department(ED) visit or hospitalization. In addition, negative binomial regression was used to examine the association between outpatient visits and frailty. Our results showed that the frail elderly increased the ED visit and the number of outpatient visit significantly after controlling for demographic characteristics, socioeconomic status, the number of chronic diseases, and self-rated health status. Considering that frailty is an important independent factor affecting health care utilization, more attention is required to prevent the frailty in our health care system.

• Journal of the Korean Society of Marine Environment & Safety
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• v.24 no.7
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• pp.967-972
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• 2018

The genus Chattonella belonging to the class raphidophyceae, is a harmful algal bloom species. Recently, its occurrence has been increasing and expanding along the Korean coast. Species identification of the genus Chattonella only by morphological observation is difficult due to the lack of rigid cell walls. In this study, the morphological characteristics and genetic affinity of Chattonella sp. isolated from Deungnyang Bay in 2017 were examined. We carried out single-cell isolation from field samples then sequenced three different areas using the single-cell PCR method: 1) parts of ribosomal operon, the large subunit (LSU) of the rDNA, 2) the chloroplast-encoded subunit psaA of Photosystem I, and 3) rbcL encoding the large subunit of the Rubisco gene. The cells were morphologically very similar to the general genus Chattonella ($74.0{\pm}10.1{\mu}m$ in length, $33.1{\pm}3.6{\mu}m$ in width). The three partial gene sequences were insufficient to justify distinction at the species rank. However, they clustered at 99-100 % sequence similarity with C. marina, C. marina var. antiqua and C. marina var. ovata.

• Research in Plant Disease
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• v.24 no.4
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• pp.328-331
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• 2018

A severe disease with leaf spots and necrotic symptoms was observed in cucumber (Cucumis sativus L.) seedlings in April 2018 at a nursery in Kimjae, Korea (35o 47'09.8"N 127o 2'24.3"E). The infected plants initially showed spots on water-soaked cotyledons which, at later stages, enlarged and spread to the leaves, which the lesions becoming dry and chlorotic. The symptomatic samples were collected from cucumber and the isolates were cultured on LB agar. The representative bacterial strain selected for identification showed fluorescent on King's medium B, was potato rot-positive, levan and arginine dihydrolase-negative, oxidase-negative and tobacco hypersensitivity-positive in LOPAT group 2 as determined by LOPAT tests. A pathogenicity test was carried out on a 3-week-old cucumber. After 3 days of inoculation, leaf spots and necrotic symptoms appeared on the cucumber, similar to the originally infected plants. The infecting bacterial strain was identified as Pseudomonas viridiflava, by 16S rDNA sequence analysis. This is the first report of leaf spot diseases on cucumber caused by P. viridiflava.

• Journal of the Korea institute for structural maintenance and inspection
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• v.23 no.1
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• pp.27-35
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• 2019

The existing middle-long span railway bridge has been mainly applied to steel box girder bridges. However, the steel box girder bridges have disadvantages in securing the space under the bridge, and the main girder is made of a thin plate box shape, resulting in a ringing noise due to the vibration. Many complaints about noise have been raised. For this reason, there is a need for the development of long railway bridges that can replace steel box girder bridges. In this paper, the characteristics of the steel composite railway bridge currently developed were introduced and a time history analysis was conducted using MIDAS Civil reflecting the speed of KTX load for 40m and 50m bridges. In addition, from the analysis results, the dynamic behavior of target bridges were verified and it was examined whether they meet the dynamic performance criteria proposed in the railway design standards. As a result, all of the bridges under review satisfied the dynamic safety criteria, however, in case of 40m of span, the vertical acceleration value was very large. In order to solve this problem, authors proposed the improvement plan and corrected the cross section to confirm that the vertical acceleration decreased.