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http://dx.doi.org/10.11625/KJOA.2014.22.4.855

The Isolation and Characterization of the Antagonistic Microorganisms, Serratia marcescens-YJK1, for Major Pathogens on Paprika  

Yang, Soo-Jeong (전북대학교 농생물학과)
Kim, Hyung-Moo (전북대학교 농생물학과 식물의학연구센터)
Ju, Ho-Jong (전북대학교 농생물학과 식물의학연구센터)
Publication Information
Korean Journal of Organic Agriculture / v.22, no.4, 2014 , pp. 855-868 More about this Journal
Abstract
Synthetic agro-chemicals have been widely used to control diseases on paprika but these days negative attention has been increasing to use of them because of several adverse effects. This research was conducted to isolate and to characterize the antagonistic microorganism to control major paprika diseases, gray mold rot, fruit and stem rot, phytophthora blight, sclerotium rot, and wilt disease. Analysis of the fatty acid and analysis of the 16S rDNA gene sequence revealed that YKJ1 isolated in this research belongs to a group of Serratia marcescens. Specially, 16S rDNA gene sequence of YKJ1 showed 99% of sequence similarity with S. marcescens. Observation through the optical microscope revealed that YKJ1 suppressed the spore germination and the hyphal growth of pathogens. YKJ1 treatment on pathogens induced marked morphological changes like hyphal swelling and degradation of cell wall. In the case of phytophthora blight, the zoosporangium formation was restrained. S. marcescens found in this study call as S. marcescens-YKJ1 and it may be valuable as one of biological control agents against major diseases of paprika in the future even though it is require to be tested with more study on field test.
Keywords
antifungal microorganism; biological control; paprika; serratia marcescens;
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