Browse > Article
http://dx.doi.org/10.5423/RPD.2008.14.1.071

Some Properties of an Isolate of Peanut stunt virus Isolated from White Clover (Trifolium repens L.)  

Jung, Goo-Ho (Division of Bio-resources Technology, Kangwon National University)
Jeon, Yong-Woon (Division of Bio-resources Technology, Kangwon National University)
Choi, Jang-Kyung (Division of Bio-resources Technology, Kangwon National University)
Hong, Jin-Sung (Division of Environmental and Life Sciences, Seoul Women's University)
Ryu, Ki-Hyun (Division of Environmental and Life Sciences, Seoul Women's University)
Lee, Sang-Yong (Department of Forest Resources Protection, Kangwon National University)
Publication Information
Research in Plant Disease / v.14, no.1, 2008 , pp. 71-75 More about this Journal
Abstract
An isolate of Peanut stunt virus (PSV), named as Tr-PSV, was isolated from white clover (Trifolium repens L) showing mosaic symptom. Tr-PSV systemically infected all plants tested in the Nicotiana spp. and induced local lesions on inoculated leaves of Chenopodium amaranticolor. However, Tr-PSV induced typical mosaic symptoms as ER-PSV on Vigna unguiculata 5 to 6 days after inoculation, while Fny-CMV used as a control virus of Cucumovirus produced local lesions on inoculated leaves. In dsRNA analysis, Tr-PSV consisted of four dsRNAs, but satellite RNA was not detected. The cDNA of coat protein gene of Tr-PSV was amplified by RT-PCR using a Cucumovirus-specific single pair primers that designed to amplify a DNA fragment of approximately 950 bp. By restriction mapping analysis using RFLP of the RT-PCR products and by serological properties of gel diffusion test, Tr-PSV belongs to a typical member of PSV subgroup I. This is the first report on the occurrence of PSV in white clover in Korea.
Keywords
Host reaction; Peanut stunt virus subgroup I; RFLP; White clover;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Dodds, J. A. 1993. DsRNA in diagnosis. In : Diagnosis of Plant Virus Diseases, ed. by R.E.F. Matthews. pp. 273-295. CRC Press, Boca Raton, USA
2 Milbrath, G. M. and Tolin, S. A. 1977. Identification, host range and serology of peanut stunt virus isolated from soybean. Plant Dis. Rep. 61: 637-640
3 Morris, T. J. and Dodds, J. A. 1979. Isolation and analysis of double-stranded RNA from virus-infected plant and fungal tissue. Phytopathology 69: 854-858   DOI
4 류갑희, 이순형, 라용준. 1986. 흰 토끼풀에서 분리한 강낭콩황 반모자이크 바이러스의 동정. 한국식물병리학회지 2: 48-52
5 Barnett, O. W. and Gibson, P. B. 1975. Identification and prevalence of white clover viruses and the resistance of Trifolium species to these viruses. Crop Sci. 15: 32-37   DOI
6 Brunt, A. A., Crabtree, K., Dallwitz, M. J., Gibbs, A. J., Watson, L. and Zurcheri, E. J. 1996. Plant Virus Online: Descriptions and Lists from VIDE Database (Version 20th). Aug. 1966
7 Choi, S. K., Choi, J. K., Park, W. M. and Ryu, K. H. 1999. RTPCR detection and identification of three species of cucumoviruses with a genus-specific single pair of primers. J. Virol. Methods 83: 67-73   DOI   ScienceOn
8 Echandi, E. and Hebert, T. T. 1971. Stunt of beans incited by peanut stunt virus. Phytopathology 61: 328-330   DOI
9 Fauquet, C. M. and Mayo, M. A. 2001. The Bromoviridae. In: Virus taxonomy (7th Report of ICTV). Arch. Virol. 146: 189- 194   DOI
10 Yan, L., Zu, Z. Y., Goldbach, R., Kunrong, C. and Prins, M. 2005. Nucleotide sequence analyses of genomic RNAs of peanut stunt virus Mi, the type strain representative of a novel PSV subgroup from China. Arch. Virol. 150: 1203-1211   DOI
11 Mink, G. I., Silbernagel, M. J. and Saksena, K. N. 1969. Host range, purification and properties of the western strain of peanut stunt virus. Phytopathology 59: 1625-1631
12 Choi, J. K., Kim, H. J., Hong, J. S., Kim, D. W. and Lee, S. Y. 1998. Identification and differentiation of cucumber mosaic virus isolates in Korea. Korean J. Plant Pathol. 14: 7-12
13 Xu, Z., Garnet, O. W. and Gibson, P. B. 1986. Characterization of peanut stunt virus strains by host reactions, serology, and RNA patterns. Phytopathology 76: 390-395   DOI
14 Singh, Z., Jones, R. A. C. and Jones, M. G. K. 1995. Identification of cucumber mosaic virus subgroup I isolates from banana plants affected by infectious chlorosis disease using RT-PCR. Plant Dis. 79: 713-716   DOI   ScienceOn
15 Hu, C. C., Aboul-Ata, A. E., Naidu, R. A. and Ghabrial, S. A. 1997. Evidence for the occurrence of two distinct subgroups of peanut stunt cucumovirus strains: Molecular characterization of RNA3. J. Gen. Virol. 78: 929-939   DOI
16 Miller, L. I. and Troutman, J. L. 1966. Stunt disease of peanuts in Virginia. Plant Dis. 50: 139-143
17 Kaper, J. M. and Waterworth, H. E. 1981. Cucumoviruses, In: Handbook of Plant Virus Infections-Comparative Diagnosis (Kurstak, E. Ed.), Elsevier/North-Holland Biomedical Press, Amsterdam, pp. 257-332
18 Bang, J. H., Choi, J. K. and Lee, S. Y. 2006. Characterization of Peanut stunt virus isolated from black locust tree (Robiniapseudo-acacia L.). Plant Pathol. J. 2: 125-130   과학기술학회마을   DOI   ScienceOn