• Research in Plant Disease
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• v.21 no.2
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• pp.99-102
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• 2015

Cucumber mosaic virus (CMV) is one of the most destructive viruses in chilli pepper. An isolate of CMV was obtained from the chilli pepper cv. Chungyang showing top necrosis symptom in 2013 and designated as CMV-GTN. CMV-GTN was compared with the well-characterized isolate, CMV-Ca-P1, by investigating their amino acid sequences of the coat protein (CP) and biological reactions in several host plants. The CP of CMV-Ca-P1 composed of 217 amino acids but that of CMV-GTN composed of 218 amino acids by including additional valine in the $57^{th}$ amino acid position. Amino acid sequence similarity of the CP gene among CMV-GTN and other CMV isolates recorded in the GeneBank database ranged from 96% to 99%. CMV-GTN was selected as a representative isolate to screen the resistance pepper cultivars to CMV because it was highly pathogenic to tomatoes and peppers upon biological assays. The virulence of CMV-GTN was tested on 135 pepper cultivars which has been bred in Korea and compared with that of CMV-Ca-P1. Only the cv. Premium was resistant and three cvs. Hot star, Kaiser, and Good choice were moderately resistant to CMV-GTN, whereas two cvs. Baerotta and Kaiser were resistant to CMV-Ca-P1.

• Archives of Plastic Surgery
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• v.38 no.5
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• pp.576-584
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• 2011

Purpose: The shape and location, the amount of the wound and the characteristics of the remaining tissues are known to influence wound contraction. The previous studies using small animals have not been an appropriate model because the wound healing mechanisms and skin structures are different from those of the human. The purpose of this study is to evaluate wound contraction according to the shape and location of the wound using a $Micropig^{(R)}$. Methods: Four $Micropigs^{(R)}$ (Medikinetics, Pyeongtaek, Korea) that were 10 months of age weighed 25 kg were used. Full thickness skin defects were made by clearing all the tissues above the fascial layer in the shape of square, a regular triangle and a circle of 9 $cm^2$ each on the back around the spine. Eight wounds were created on the back of each pig, 50 mm apart from each other. The randomly chosen wound shapes included 11 squares, 11 regular triangles, and 10 circles. Wound dressing was done every other day with polyurethane foam. The wound size was measured using a Visitrak $Digital^{(R)}$ (Smith & Nephew, Hull, UK) on every other day after surgery from day 2 to day 28. A biopsy was performed on day 3, and 1, 2, 3 and 4 weeks to investigate the degree of acute and chronic inflammation, the number of microvesssel and myofibroblast density using H & E stain and immunohistochemistry. The wound contraction rate was calculated to figure out the differences among each of the shapes and the locations. Results: The ultimate shape of the circle wound was oval, and that of the regular triangle and square were stellate. The maximum contraction rate was obtained on 8 to 10 days for all the shapes, which corresponds with the immunohistochemical finding that myofibroblast increases in the earlier 2 weeks whereas it decreases in the later 2 weeks. Epithelialization was seen in the wound margin on day 7 and afterwards. The final wound contraction rates were highest for the regular triangle shapes; however, there were no statistically significant differences. The wound contraction rates by locations showed statistically significant differences. The wound in the cephalic area presented more contractions than that of the wounds in the caudal area. Conclusion: The location of a wound is more important factor than the wound shape in wound contraction.

• Journal of the Korean Society for Library and Information Science
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• v.51 no.1
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• pp.175-199
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• 2017

Children's literature has a significant impact on children's intelligence and emotional development. The interest in children's literature is increasingly growing with the expectation of the award winning books and the educational values of children's books. Although the interest has brought about a quantitative increase in the domestic children's books, it has difficulty selecting high-quality children's books among various books. In such an environment, the Children's Literature Award will provide an authoritative source of choice, which will help guide the selection of quality books to those who want to read children's books. In addition to this trend, the library should check the status of winning works and incorporate them into new collection development policies. The purpose of this study is to present the direction of the library book development policy of the public library. For this purpose, 10 public libraries in Seoul, Korea were selected and analyzed quantitatively and qualitatively through the collection status of the children's literature winning books, loan status, turnover, Bonn's use Factor, and Trueswell's 80/20 rule. Based on the results of this analysis, we would like to suggest some issues to be reflected in library development policy of the future.

• Journal of the Korean Society of Food Science and Nutrition
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• v.35 no.9
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• pp.1194-1199
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• 2006

Effects of Korean traditional soybean fermented foods on weight reduction and lipid lowering activities were studied using Spraque-Dawley (SD) rats fed a high fat diet. The rats were raised for four weeks after adaptation period on either a normal diet (ND, based on the AIN-93 diet), high fat diet (HFD, supplemented with 12% lard oil in the ND), or diets containing 10% of freeze dried Doenjang, Chungkukjang, Kochujang, or Samjang to HFD. The final weight, food efficiency ratio (FER) and the weight of adipose tissue were decreased significantly by the consumption of Doenjang and Samjang, compared to HFD (p<0.05). The contents of cholesterol and triglyceride increased by HFD were reduced by the fermented foods in liver and perirenal fat tissues of the rats, especially Doenjang and Samjang diets showed high lipid lowering activity. And TG and cholesterol contents in the plasma serum were simillar trend to liver and perirenal fat tissue. These results suggested that Korean traditional soybean fermented foods, especially Doenjang showed the highest weight reduction and lipid lowering activities of the rats fed high fat diet.

• Korean Journal of Microbiology
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• v.53 no.4
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• pp.265-272
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• 2017

Some bacteria with different mechanisms for hydrocarbon degradation were isolated from oil-contaminated soils in Korea. Isolate Acinetobacter calcoaceticus SL1 showed biosurfactant- producing activity in oil-spreading test, and it exhibited a good emulsifying activity of 43.6 and 54.5% for diesel oil and n-hexane, respectively. It also has high cell surface hydrophobicity which can make it easily attaches to hydrocarbons and degrade them. It degraded 100% of 1,000 mg/L of n-octadecane and naphthalene, respectively in 3 days, 72.3% of 1,000 mg/L diesel oil in 7 days and 78.0% of 10,000 mg/L diesel oil in oil-contaminated soil during 28 days. Isolated strains Bacillus amyloliquefaciens S10 and B. subtilis GO9 can produce biosurfactant and formed 6.34 and 2.5 cm diameter of clear zones, respectively in oil-spreading test. Surface tension of their culture supernatant reduced from 74.6 to 34.4 and 33.3 mN/m, respectively during incubation, and critical micelle concentrations of culture supernatants were 2.0 and 5.9%, respectively. Consortium of A. calcoaceticus SL1 and B. amyloliquefaciens S10 degraded 77.8% of 10,000 mg/L diesel oil in 3 days, which indicated more efficient oil degradation than that by A. calcoaceticus SL1 alone. If these bacteria were applied together as a consortium to oil-contaminated sites, they may show a high removal rate of petroleum hydrocarbons.

• Journal of Korean Classical Literature and Education
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• no.33
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• pp.5-44
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• 2016

This study focuses on Ko woo-young (1939-2005), a Korean representative cartoonist. I analyzed his work Nolbudyeon (1988), which is a retelling of Heungbu and Nolbu, a traditional Korean story. I consider Ko woo-young a creative observer who has popularized his perspective in modern society. His work is a good example of how a traditional folktale can be made relevant in modern society. I used three methods of adaptation to differentiate Nolbudyeon from Heungbu story: construction of events, characterizing, and space-time background. First, to aid character development, Nolbudyeon includes a prologue that focuses on conflicts between brothers with different personalities. At the same time, the ambiguous ending could be a response to Heungbu story, which has a didactic theme. Second, I found that the new characters, Nolsun and Yeonsaengwon, enhanced the differences between Heungbu and Nolbu by playing the role of mediators. Also, in Nolbudyeon, both Heungbu and Nolbu had positive as well as negative elements, in contrast with the original story's traditional point of view on good and evil. Finally, by exploring the space-time of Nolbudyeon, we can see that its world is combined with the contemporary world. In other words, Nolbu and Heungbu, though outwardly traditional, have a modern outlook. Therefore, readers can recognize that Nolbudyeon is a metaphor for modern life rather than just an old story.

• Journal of the Korea Convergence Society
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• v.9 no.11
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• pp.299-306
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• 2018

Changes in demographic and economic conditions affect corporate strategy, labor market and social welfare. The purpose of this study is to examine the effects of changes in population and economic situation on library use and to examine the income effect of library use. In order to achieve the purpose of this study, we analyzed the 10 year fixed library of public library statistics and statistical census data from 2008 to 2017 using the panel fixed effect model using the data of regional gross production and unemployment rate. And the changes in the economic and demographic characteristics of the region are used to confirm the regularity of the relationship between the changes in the usage situation of the libraries belonging to a certain region and the changes in the economic and demographic conditions in the respective regions. As a result of the analysis, the changes in the economic environment did not significantly affect the number of visitors to the library and the number of library users, but it was an important factor in the number of borrowers. This study is significant in that it shows how the economic environment changes affect the library. The result of this study is expected to be a theoretical basis for establishing the service policy of public libraries.

• The Journal of Korean society of community based occupational therapy
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• v.8 no.3
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• pp.59-67
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• 2018

Objective : This study was conducted to investigate the effects of occupation-based group cooking program on executive function for children in community children's center. Methods : The children who participated in the study were 7 to 9 years old. This study was based on a one-group pre-posttest design, which the group cooking program included making of sandwiches, frog-shaped hamburgers, patbingsu, sausage gimbap, and flowerpot cupcake. This study consisted of five activity sessions, which each session lasted for 120 minutes. Effectiveness of the program was measured by using the STROOP Color and Word Test, Children's Color Trails Test and Assessment of Motor and Process Skills. Results : The differences between pre and posttest scores on STROOP Color and Word Test and the process skill score of Assessment of Motor and Process Skills were statistically significant. There was no difference significantly between pre and posttest scores in Children's Color Trails Test-2. Conclusion : This study demonstrated that the group cooking program intervention has effects on cognitive inhibition executive function and performance executive function of children in the community children's center. This study suggests that occupation-based intervention can be applied to various clients in the community setting.

• Korean Journal of Clinical Laboratory Science
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• v.51 no.1
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• pp.64-70
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• 2019

Dirofilaria immitis (D. immitis) is a filarial nematode that causes cardiopulmonary dirofilariasis in dogs. In the late stages of infection, infected dogs show one or more symptoms and advanced heart disorder with perivascular inflammation. To detect D. immitis specifically and efficiently in the early stages of infection, a duplex TaqMan qPCR assay was developed based on previous studies using primers and probes specialized to detect D. immitis cytochrome c oxidase subunit I (COI) and dog glyceraldehyde-3-phosphate dehydrogenase (GAPDH). As positive controls, plasmid DNAs were constructed from D. immitis COI or dog GAPDH and a TA-cloning vector. Simplex and duplex TaqMan qPCR assays were performed using the specific primers, probes, and genomic or plasmid DNA. The duplex reaction developed could detect D. immitis COI and dog GAPDH in the same sample simultaneously after optimization of the primer concentrations. The limit of detection was 25 copies for the simplex and duplex assays, and both showed good linearity, high sensitivity, and excellent PCR efficiency. The duplex assays for pathogen detection reduce the costs, labor, and time compared to simplex reactions. Therefore, the duplex TaqMan qPCR assay developed herein will allow efficient D. immitis detection and quantification from a large number of samples simultaneously.

• Research in Plant Disease
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• v.24 no.4
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• pp.285-291
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• 2018

Two isolates of Strawberry mild yellow edge virus were newly isolated in strawberry (Fragaria x ananassa) cultivar Selhyang and Kamhong from Korea. The complete nucleotide sequence of the coat protein (CP) of two Korean isolates were determined and analyzed. Sequence identity of nucleotide and amino acid between SH and KH isolates was 90.4% and 95.5%, respectively. The comparison of three Korean isolates including previously reported KNS1 with 45 SMYEV sequences from other countries deposited in GenBank database revealed an identity ranging from 81.2% to 100%. The phylogenetic analysis of CP of all SMYEV isolates showed the five subgroups (I-V), with Korean isolates being divided into two different subgroups. The isolates KH and KNS1 were included in subgroup I, whereas SH was included in subgroup IV which is new phylogenetic subgroup. Genetic diversity analysis indicated that new subgroup had greater variability and nucleotide diversity between subgroups resulted in values ranging from 0.0863 to 0.18004. This report represents the first molecular characterization of SMYEV isolates from Korea.