Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Characteristics of Cucumber mosaic virus-GTN and Resistance Evaluation of Chilli Pepper Cultivars to Two Cucumber mosaic virus Isolates

고추에서 분리한 오이모자이크바이러스(CMV-GTN)의 특성과 고추 품종의 저항성 평가

  • Choi, Gug-Seoun (Horticulture & Herbal Environmental Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Kwon, Sun-Jung (Horticulture & Herbal Environmental Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Choi, Seung-Kook (Horticulture & Herbal Environmental Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Cho, In-Sook (Horticulture & Herbal Environmental Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Yoon, Ju-Yeon (Horticulture & Herbal Environmental Division, National Institute of Horticultural & Herbal Science, Rural Development Administration)
  • 최국선 (농촌진흥청 국립원예특작과학원 원예특작환경과) ;
  • 권선정 (농촌진흥청 국립원예특작과학원 원예특작환경과) ;
  • 최승국 (농촌진흥청 국립원예특작과학원 원예특작환경과) ;
  • 조인숙 (농촌진흥청 국립원예특작과학원 원예특작환경과) ;
  • 윤주연 (농촌진흥청 국립원예특작과학원 원예특작환경과)
  • Received : 2015.02.23
  • Accepted : 2015.04.21
  • Published : 2015.06.30
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Abstract

Cucumber mosaic virus (CMV) is one of the most destructive viruses in chilli pepper. An isolate of CMV was obtained from the chilli pepper cv. Chungyang showing top necrosis symptom in 2013 and designated as CMV-GTN. CMV-GTN was compared with the well-characterized isolate, CMV-Ca-P1, by investigating their amino acid sequences of the coat protein (CP) and biological reactions in several host plants. The CP of CMV-Ca-P1 composed of 217 amino acids but that of CMV-GTN composed of 218 amino acids by including additional valine in the $57^{th}$ amino acid position. Amino acid sequence similarity of the CP gene among CMV-GTN and other CMV isolates recorded in the GeneBank database ranged from 96% to 99%. CMV-GTN was selected as a representative isolate to screen the resistance pepper cultivars to CMV because it was highly pathogenic to tomatoes and peppers upon biological assays. The virulence of CMV-GTN was tested on 135 pepper cultivars which has been bred in Korea and compared with that of CMV-Ca-P1. Only the cv. Premium was resistant and three cvs. Hot star, Kaiser, and Good choice were moderately resistant to CMV-GTN, whereas two cvs. Baerotta and Kaiser were resistant to CMV-Ca-P1.

오이모자이크바이러스(CMV)는 고추에 심한 피해를 주는 바이러스이다. 2013년 고추 품종 청양의 선단부위에 괴저증상이 나타난 식물체로부터 CMV를 순수 분리하였고 이 분리주를 CMV-GTN으로 명명하였다. CMV-GTN과 기존에 특성이 잘 알려진 CMV-Ca-P1을 대상으로 외피단백질의 아미노산 서열과 여러 기주식물에서 생물적 반응을 비교하였다. CMV-Ca-P1의 외피단백질 아미노산은 217개로 구성되어 있었으나, CMV-GTN은 아미노산 서열 57번째에 발린이 추가된 218개로 구성되어 있었다. CMV-GTN과 GeneBank에 등록된 다양한 CMV 분리주들의 외피단백질유전자 유사성은 96-99%였다. 생물 검정에서 토마토와 고추에서 병원성이 강하게 발현되는 CMV-GTN을 고추 품종에 대한 저항성 스크린을 위하여 선발하였다. 고추 시판 품종 135종에 대하여 CMV-GTN으로 검정하였고 CMV-Ca-P1과 저항성 반응을 비교하였다. 그 결과 CMV-GTN에 대하여 저항성 반응을 표현한 품종은 프레미엄, 중도 저항성 품종은 핫스타, 카이저, 굿쵸이스였다. 한편 CMV-Ca-P1에 대한 저항성 품종은 베로따와 카이저였다.

Keywords

References

  1. Canto, T., Proir, T. A., Hellwald, K. H., Oparka, K. and Palukaitis, P. 1997. Characterization of Cucumber mosaic virus. IV. Movement protein and coat protein are both essential for cell-to-cell movement of Cucumber mosaic virus. Virology 237: 237-248. https://doi.org/10.1006/viro.1997.8804
  2. Cho, J. D., Kim, J. S., Lee, S. H. and Choi, G. S. 2007. Viruses and symptoms on peppers, and their infection types in Korea. Res. Plant Dis. 13: 75-81. (In Korean) https://doi.org/10.5423/RPD.2007.13.2.075
  3. Choi, G. S., Kim, J. H., Lee, D. H., Kim, J. S. and Ryu, K. H. 2005. Occurrence and distribution of viruses infecting pepper in Korea. Plant Pathol. J. 21: 258-261. https://doi.org/10.5423/PPJ.2005.21.3.258
  4. Ding, S.W., Anderson, B. J., Haase, H. R. and Symons, R. H. 1994. New overlapping gene encoded by the Cucumber mosaic virus genome. Virology 198: 593-601. https://doi.org/10.1006/viro.1994.1071
  5. Green, S. K. and Kim, J. S. 1991. Characteristics and control of viruses infecting peppers: a literature review. Asian Vegetable Research and Development Center. Technical Bulletin No. 18. 60 pp.
  6. Hayes, R. J. and Buck, K. W. 1990. Complete replication of a eukaryotic virus RNA in vitro by a purified RNA-dependent RNA polymerase. Cell 63: 363-368. https://doi.org/10.1016/0092-8674(90)90169-F
  7. Lee, M. Y., Lee, J. H., Ahn, H. I., Yoon, J. H., Her, N. H., Choi, J. K., Choi, G. S., Kim, D. S., Harn, C. H. and Ryu, K. H. 2006. Identification and sequence analysis of RNA3 of a resistance-breaking Cucumber mosaic virus isolate on Capsicum annuum. Plant Pathol. J. 22: 265-270. https://doi.org/10.5423/PPJ.2006.22.3.265
  8. Morroni, M., Thompson, J. R. and Tepfer, M. 2008. Twenty Years of Transgenic Plants Resistant to Cucumber mosaic virus. MPMI 21: 675-684. https://doi.org/10.1094/MPMI-21-6-0675
  9. Palukaitis, P. and Garcia-Arenal, F. 2003. Cucumoviruses. Adv. Virus. Res. 62: 241-323. https://doi.org/10.1016/S0065-3527(03)62005-1
  10. Peden, K. W. C. and Symons, R. H. 1973. Cucumber mosaic virus contains a unctionally divided genome. Virology 53: 487-492. https://doi.org/10.1016/0042-6822(73)90232-8
  11. The Korean Society of Plant Pathology. 2009. List of plant diseases in Korea. 5th ed., Suwon, Korea. 853 pp. (In Korean)

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