• Environmental Mutagens and Carcinogens
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• v.10 no.2
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• pp.119-126
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• 1990

본 최기형시험은 엘라스타제가 임신토끼와 태자에 미치는 영향을 조사할 목적으로 수행되었다. 시험은 임신된 New Zealand White 종의 토끼를 2개의 대조군 (제 1, 제 2대조군)과 3개의 투여군 (제 1, 제 2, 제 3투여군)으로 나누어 실시되었다. 제 1, 제 2, 제 3투여군에는 각각 엘라스타제 1, 2, 12, 120mg/kg을 임신 6일부터 18일까지 연일 경구투여하였으며, 같은 기간동안 제 1대조군과 제 2대조군에는 각각 10% skim milk 수용액과 ethylene thiourea 40mg/kg을 경구투여하였다. 임신 29일에 모든 모동물을 도살하여 모체와 태자를 검색하였다. 시험기간중 제 2대조군에서는 사료섭취량이 유의성있게 감소되었다 (P<0.01). 시험결과 황체수, 착상수, 생존태자수, 태자의 성비, 태자 및 태반중량에 있어서 각 군간 유의성있는 변화가 관찰되지 않았으며 태자의 외형검사, 애부장기 및 골격 검사에서도 군간 유의성있게 변화가 관찰되지 않았다. 이상의 결과로 미루어 본 시험에서 채택한 엘라스타제의 용량은 토기에 있어서 최기형 유발능이 없는 것으로 판단된다.

• Journal of the Korean Society of Food Science and Nutrition
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• v.36 no.4
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• pp.439-446
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• 2007

This study was performed to investigate the effect of long-term administration of Saengshik on growth parameters of growing rats. Male Sprague-Dawley rats were fed on AIN-93G basal diets for 12 weeks and assigned to the following groups: rats administrated orally with Saengshik at the dose of 1g/kg/day (1xJS ), 2g/kg/day (2xJS), 4g/kg/day (4xJS) and distilled water (Control). Rats were sacrificed at 4, 8, 12 weeks after oral administration. Bone mineral density (BMD) and bone mineral contents (BMC) were measured by PIXImus densitometry and serum insulin-like growth factor-1 (IGF-1) concentration were determined by using EIA method. Body weight and food intake did not show significant changes within groups for 12 weeks. Physical longitudinal growth indexes, body length and femur length were significantly increased in Saengshik-administered groups at 12 weeks, in which BMD and BMC also significantly increased. Also, in blood IGF-1 level, Saengshik-administered groups were remarkedly higher than control group at 4 week (p<0.001), in which significantly higher at 8 week and 12 week. These results suggest a close relation between administration of Saengshik and increment of longitudingal bone growth. Therefore, as the result of this study, it could be expected that the administration of Saengshik for 12 weeks is helpful to the increase of longitudinal growth and growth factors in rats. Furthermore, we propose that the consumption of Saengshik as dietary supplementation may promote to increase in longitudinal bone growth in growing children.

• Food Science of Animal Resources
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• v.24 no.2
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• pp.198-201
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• 2004

The studies was conducted to investigate the effect of hangover drink using natural products propolis on ethanol oxidation. The hangover drink developed using propolis concentrates and various medicinal plants. The extractions of propolis and acanthopanax senticosus was shown the highest alcohol oxidation among the combination raw. The ethanol oxidation effect were not significantly different between propolis drink and in other company products in acute alcoholic toxic doses. In the case that it was conducted oral administration with acute alcoholic toxic doses 1/3(1.8mg per 1kg body weight of mouse), propolis drink comparative products of other company was shown 0.026％ to 270 min, 0.000％ to 360 min. In conclusion, propolis drink was shown excellent alcohol oxidation in fixed dose of alcohol, but was not shown in a large quantities of alcohol.

• Korean Journal of Food Science and Technology
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• v.34 no.3
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• pp.487-492
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• 2002

Low-fat and sugar-free (LFSF) cookies were developed for patients with metabolic syndrome X, such as obesity, diabetes, coronary heart disease, and hypertention, using artificial sweeteners (mixture of aspartame and saccharin), pectin and herb extracts such as Polygonatum Odoratum (Mill) Druce, Schizandrae Fructus and Lycii Fructus, without sugar and fats. LFSF cookies were composed of 7.5 : 1 of aspartame and saccharin, 5% pectin, 49% protein, and 5% herb extracts, with reduced fat level. The values for area under the curve in oral glucose tolerance tests were significantly lower in 90% pancreatomized-(Px, n = 8) and sham - operated (Sham, n = 8) rats which consumed LFSF cookies, than the control, which consumed regular cookies. Blood glucose levels were higher and the peak levels were significantly lower in the LFSF cookies group than the control group of Px and Sham rats. Blood glucose levels of healthy female college students (n = 10) at 30 and 60 min after the consumption of 30 g LFSF and regular cookies were not different, but they were significantly lower in the LFSF-cookies group in diabetes patients (n = 10). In conclusions, LFSF cookies was considered as a good snack for diabetic patients.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.3
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• pp.611-621
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• 2016

This study examined the effects of collagen peptide intake extracted from the tilapia fish (TFCP) on UVB-induced skin in hairless mice. To cause photoaging, the hairless mice were exposed to UVB irradiation three times a week and TFCP was administered with 545 and 1090 mg/kg once a day for 12 weeks. The level of wrinkle formation, thickness of the skin, acute erythema, skin hydration, amount of hydroxyproline, expression of MMPs and filaggrin were measured. Wrinkle formation, thickness of the skin, and acute erythema were significantly lower and skin hydration was higher in the TFCP intake group compared to the UVB-control mice group. In addition, the amount of the collagen and hydroxyproline of the collagen peptide intake group were much higher than the UVB-control group. Oral administration of TFCP inhibited the expression of the MMP-3 and MMP-13 mRNA level as well as activated MMP-2 and MMP-9 induced by UV irradiation, and increased the protein expression of filaggrin in hairless mice skin. In summary, the oral administration of TFCP can inhibit wrinkle formation and protect the skin from UV-damage. Therefore, this study suggests that TFCP is a potential candidate material for skin functional foods.

• Journal of Food Hygiene and Safety
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• v.25 no.3
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• pp.263-268
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• 2010

This study was demonstrate a repeated oral dose toxicity of detoxication sulphur in 8-weeks-old female Sprague-Dawley (SD) rats. The rats were treated with dose of 0.2%, 1%, 5% detoxication sulphur and 1% sulphur of feed consumption administered for 13 weeks. To evaluate the safety of detoxication sulfur, we examined the body weight, the feed intake, the clinical signs, the ophthalmological test, the hematological and the serum biochemical analysis. We also observed the histopathological changes of liver and kidney in rats. As a result, no significant differences in body weight, feed intake, hematological examination and histopathological between control and detoxication sulphur treatment group were found. Serum biochemical results were not shown significant differences in 0.2% and 1% the treated groups compared with control group. But glucose level were decrease, also ALT and ALP level were increase in 5% treated group. All of these results indicate that 1% detoxication sulphur of feed consumption may be safety in SD rat.

• Journal of Food Hygiene and Safety
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• v.36 no.1
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• pp.77-85
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• 2021

Glucosylceramides (GluCer) are known to play an important role in both water retention and epidermal permeability barrier function in the mammalian stratum corneum. In this study, we investigated the effects of pineapple fruit extract containing glucosylceramides (PFEG) on the maintenance and recovery of skin barrier function using atopic dermatitis-induced animal models. Five-week-old male Hos:HR-1 mice were divided into four groups fed on standard diet, unsaturated fatty acids-deficient (HR-AD) diet, and HR-AD diet supplemented with 0.01% or 0.1% pineapple-GluCer. Skin barrier function was evaluated by transepidermal water loss (TEWL), dermal moisture content, moisture content of the stratum corneum and wrinkle formation. The control group (HR-AD administration group) showed increased transepidermal water loss (TEWL), while the epidermal moisture content and the moisture content of the stratum corneum slowly decreased. However, in the PFEG groups (with 0.1% or 0.01% glucosylceramide), the TEWL levels were significantly reduced at 2 weeks. The PFEG also helped maintain skin moisturizing function by significantly suppressing the decrease of the epidermal moisture content and the moisture content of the stratum corneum. These results show that the PFEG is effective for maintaining and improving the function of the skin barrier. Therefore, this study suggests that PFEG is a potential candidate material for skin functional foods.

• Journal of agricultural medicine and community health
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• v.39 no.4
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• pp.240-255
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• 2014

Objective: The purpose of this study was to identify the correlates of bone mineral density of Korean adults. Methods: This study was based on the data from the 2010-2011 Korean National Health and Nutrition Examination Survey (KNHANES, 2010~2011) V. Among 8,473 subjects of the survey, who were adults 19 years old or over with bone mineral density data, the current study excluded those whose data include a missing study variable or the response of 'I don't know', and finally analyzed the data of 5,986 subjects (2,692 males and 3,294 females). This study employed the following statistical methods of analysis: t-test, analysis of variance (ANOVA), and hierarchical multivariate linear regression analysis. Results: In multivariate analysis, in the case of men, the significant associated factors in bone mineral density were age, education level, body mass index (BMI), waist circumference, total muscle mass, exercise, and weight control; in the case of women, the significant associated factors in bone mineral density were age, education level, economic activity, BMI, total muscle mass, exercise, weight control. fat intake, uptake female hormone, menopause, and age of menarche. Conclusion: For both sexes, the strongest modifiable factor in influencing bone mineral density was total muscle mass. Therefore, to prevent osteoporosis and promote health, active health education and interventions such as regular exercise for total muscle mass increase should be implemented from an early growth period.

• Journal of Life Science
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• v.27 no.11
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• pp.1376-1380
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• 2017

This study examined how the daily administration of dried CP roots affects body-weight gains of mice. An aqueous CP extract (1 g/kg of CP-W) was orally administered to mice on a daily basis for ten weeks, and the mice's body weights and food intakes were measured throughout the period. At the end of the ten-week treatment, murine blood samples were taken and hematological and serum biochemical parameters were examined. The mice's intra-abdominal organs were weighed at necropsy. The treatment of CP-W significantly suppressed the mice's body-weight gain by about 10%. There were no changes to the food intakes of and no observable abnormal clinical signs in any of the animals throughout the experimental period. There were no significant differences between the control and CP-W mice in regards to the hematological and serum biochemical parameters and the organ weights at necropsy. The results demonstrated that the daily administration of CP-W for ten weeks suppresses body-weight gain in mice and does not change mice's food intake or cause any noticeable toxic effects.

• The Korean Journal of Pesticide Science
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• v.15 no.2
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• pp.104-113
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• 2011

We investigated the systemic effects of leaf folder (Cnaphalocrocis medinalis) resistant rice (transgenic rice, Nakdongbyeb) in Sprague-Dawley rats for 13 weeks. Leaf folder resistant rice was added to the diet at percentage levels of 0, 5 and 20 percentage/feeder and was administered for 13 weeks. The results did not show any changes in food and water intake. There were also no biologically significant changes in both body and organ weights, hematological and blood biochemical parameters, autopsy and histopathology between the treatment and control groups. Based on these results, no observed adverse effect level (NOAEL) of transgenic rice was considered to be more than 10,000 mg/kg b.w. under the conditions of the present study.