• 한국해양학회지
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• v.30 no.3
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• pp.216-226
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• 1995

Stable isotopic and elemental compositions were analyzed for well- preserved calcareous skeletons (gastropods, pectenids, brachiopods, a scaphopod, and other bivalves) of the Pleistocene Seoguipo Formation from Cheju Island, Korea. Mineralogically and texturally, aragonitic and calcitic fossils still retain their original mineralogy and microstructure. High $\delta$$\^$18/O and $\delta$$\^$13/C contents indicate that the shells have not been altered by the post-burial diagenesis. Also, this is supported by high Na and Sr contents as well as low Mn and Fe compositions of the shells. Oxygen isotopic compositions of the well preserved fossils show that the organisms had lived under the lower paleotemperature or within the isotopically enriched paleo-seawater during the deposition of the Seoguipo Formation. This result strongly implies that the Seoguipo Formation was deposited during a glacial period. The paleotemperature variation throughout the sequence during the deposition of the Sewguipo Formation was negligible.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.37 no.3
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• pp.283-288
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• 2011

Since minerals are in ionic forms in aqueous phase (like deep sea water), it is very hard to incorporate concentrated minerals in gel like cosmetic formulations which are generally composed of carbomer or acrylates polymer. In this study, we invented a salt-resistant network formulation made of silicon copolyol. It was found that the network formulation traps minerals (especially from marine) in a mesh-like silicone copolyol structure. Since the mineral-containing formulation makes a very thin film on the skin, minerals could be easily absorbed into stratum corneum. In 6 weeks in vivo test, it was found that the novel mineral formulation increased the amounts of amino acids, major components of natural moisturizing factors (NMFs), as well as levels of minerals. It was also found that the moisture contents and the brightness of the skin increased.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.43 no.4
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• pp.329-335
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• 2017

We have studied on the keratinocytes differentiation and skin barrier function using Adenophorae radix (A. radix) root extract, which was known to contain triterpenoid, saponin and starch. A. radix root extracts showed the $PPAR{\alpha}$ expression level of Wy-14,643 $0.5-1.0{\mu}M$ in CV-1 cells. The cornified envelop formation (CE) of human keratinocyte cell line (HaCaT) and normal human keratinocyte (NHK) showed a statistically significant increased compared to the control. When HaCaT cells were treated with A. radix root extract, transglutaminase (TGase-1) was significantly increased. As a result of clinical study of the simple cosmetic formulation containing A. radix root extract for about 2 weeks, TEWL values were significantly decreased and water contents were increased. The ceramides, which were obtained from the inner forearm, were also significantly increased statistically. We suggest that the A. radix root extract can be used as a preventive and therapeutic agent for skin diseases such as dry skin and atopy.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.47 no.2
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• pp.171-178
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• 2021

In this study, exosomal-like nano-vesicles derived from probiotics were isolated and various physiological activities were evaluated on the skin. This study show that Lactococcus lactis subsp. lactis (LL) are incubated, and then isolated LL derived exosomal nanovesicles (LVs) at the range of 70 ~ 200 nm by high-pressure homogenizer and ultrafiltration. The vesicle numbers were an average of 1.81 × 10¹¹ particles/mL. This study finds out the bacterial nanovesicles' beneficial effect on the skin. Fibrillin (FBN1) gene expression increased by 23% in fibroblast cells. Fibronectin (FN1) and filaggrin (FLG) gene expression increased by 65% and 400% in keratinocytes. We could see that cornified envelope (CE) formation ability was increased by 30% compared to the control group. Furthermore, collagen type I alpha 1 (COL1A1) protein expression increased by 83% compared to the UV-irradiated control group. These results suggest that LVs could help skin barrier improvement and used as an ingredient for cosmetics or pharmaceuticals.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.48 no.1
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• pp.33-38
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• 2022

Ultraviolet B (UVB) damages DNA residues in skin keratinocytes. In particular, the formation of cyclobutane pyrimidine dimers (CPD), a pyrimidine residue damage in DNA, is considered a representative indicator of skin photoaging. In this study, we confirmed defensive effect of Glycyrrhiza glabra (G. glabra) extract against UVB induced DNA damage. First of all, we confirmed UVB dependent amount of CPD formation in human keratinocyte cell line. UVB induced CPD was decreased by G. glabra extract by dose dependent manner. In addition, it was confirmed that the expression of mRNA of DNA damage recovery factors was increased by G. glabra extract. Consequently, through this study, it was possible to confirm the DNA protection effect of G. glabra extract in skin keratinocytes.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.3 no.3
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• pp.112-123
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• 1998

The objectives of this study are to investigate the trace elemental and stable isotopic compositions of aragonitic mollusk shells, such as Gomphina veneriformis melanaegis, Mytilidae and Umbonium coastatum, from the eastern coast of Korean peninsula and Cheju Island, and to compare their variations with latitude, that is, the temperature of the ambient seawater at which the skeletons grew. The Mg compositions of Gomphina veneriformis melanaegis tend to decrease with increasing water temperature, whereas those of Mytilidae and Umbonium coastatum do not show any trend. Sr compositions of Mytilidae tend to increase with increasing water temperature, whereas those of Gomphina veneriformis melanaegis decrease. Fe compositions of Umbonium coastatum tend to decrease with increasing water temperature, whereas those of Gomphina veneriformis melanaegis increase. Fe compositions of Mytilidae do not show any trend with water-temperature variations. Ba compositions of all the skeletons do not show any trend with water-temperature variations. Cd compositions of Mytilidae and Umbonium coastatum do not show any trend with increasing water temperature, whereas those of Gomphina veneriformis melanaegis increase. Cu compositions of Gomphina veneriformis melanaegis increase with increasing water temperature, whereas Mytilidae and Umbonium coastatum do not show any trend. Pb compositions of Umbonium coastatum do not show any tend with increasing water temperature, but those of other skeletons increase. Zn compositions of Mytilidae tend to decrease with increasing water temperature, whereas those of Gomphina veneriformis melanaegis increase. Zn compositions of Umbonium coastatum do not show any trend with increasing water temperature. Estimated water temperatures from oxygen isotopic data of all the skeletons are higher than the range of water temperature of the shallow seawater of the East Sea and around Cheju Island. The oxygen isotopic compositions of all the skeletons are well clustered, but they do not show any trend with latitude. Therefore, this could reflect that the organisms may not have secreted their shells in oxygen isotopic equilibrium with ambient seawater due to vital effect. Thus, isotopic compositions of all the organisms in this study may not be suitable for paleotemperature estimate.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.3 no.3
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• pp.124-141
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• 1998

The objectives of this study are to investigate the trace (minor) elemental and isotopic compositions of calcitic skeletons, such as barnacle, echinoid, branching and encrusting calcareous algae and oyster, from the eastern coast of Korean peninsula and Cheju Island, and to compare their variations with latitude, that is, the temperature of ambient seawater at which the skeletons grew. Articulated and encrusting red algae are composed of high-Mg calcite (7-21 mol% $MgCO_3$). Echinoids are also composed of high-Mg calcite (7-15 mol% $MgCO_3$). Whereas barnacles are composed of low-Mg calcite (1-5 mol% $MgCO_3$). The Mg compositions of articulated red algae, barnacle and oyster tend to increase with increasing water temperature, whereas those of encrusting red algae and echinoid do not show any trend. Sr compositions of articulated red algae, echinoid and barnacle tend to decrease with increasing water temperature, whereas those of encrusting red algae and oyster do not show any trend. Mn compositions of articulated red algae and encrusting red algae decrease with an increase of water temperature, whereas those of echinoid and barnacle do not show any trend. Fe compositions of articulated red algae tend to decrease with increasing water temperature, whereas those of encrusting red algae and oyster increase. Fe compositions of echinoid and barnacle do not show any trend with water temperature variations. Ba compositions of echinoid and low-magnesium calcitic skeletons do not show any trend with water temperature variations. Ba compositions of articulated red algae tend to decrease with increasing water temperature, whereas those of encrusting red algae increase. Cd and Pb compositions of all the skeletons tend to increase with increasing water temperature. Cu compositions of encrusting red algae increase with increasing water temperature, whereas articulated red algae, echinoid, barnacle and oyster do not show any trend. Zn compositions of high-magnesium calcitic skeletons and low-magnesium calcitic skeletons do not show any trend. Estimated water temperatures from oxygen isotopic data of all the skeletons except for the barnacle arc higher than the range of temperature of the shallow seawater of the East Sea and around Cheju Island. The oxygen isotopic compositions of oyster and echinoid are well clustered, and they do not show any trend with increasing water temperature. Therefore, this could reflect that the organisms have not secreted their shells in oxygen isotopic equilibrium with ambient seawater due to vital effect. Thus, the oxygen isotopic composition of barnacle can potentially be utilized for paleotemperature estimate whereas those of other organisms in this study may not be useful.

• Environmental Analysis Health and Toxicology
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• v.20 no.3 s.50
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• pp.229-235
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• 2005

태양광선,특히 자외선 B에 대한 환경적 노출은 편평세포암과 기저세포암을 포함하는 흑색선종 이외의 피부암과 크게 관련된다고 알려져 있다. 염기 류신 지퍼계 전사조절인자인 CChAT/enhancer binding protein-alpha는 표피 각질형성세포에서 다량으로 발현되었고, 각질형성세포의 증식을 억제하며 피부암 발생을 억제하는 유전자로서의 역할이 암시된 바 있다. 최근 자외선 B가 각질형성세포에서 p53에 의한 CCAAT/enhanrer binding protein-alpha의 발현을 강력하게 유도한다는 것이 보고되었다. 이러한 CCAAT/enhancer binding protein-alpha 단백질 발현의 유도는 세포 성장 억제 세포고사와 함께 일어났다. 이 연구는 glycogen synthase kinase-3 길항제가 자외선 B에 의한 CCAAT/enhancer binding protein-alpha 유도를 억제하며 변이 kinase-불활성 GSK의 강제 발현은 자외선 B가 CCAAT/enhancer binding protein-alpha전사조절부위 활성의 증가를 억제한다는 것을 보여주었다. 즉 자외선 B에 의한 CCAAT/enhancer binding protein-alpha의 유도가 p53과 활성 glycogen synthase kinase-3에 의한 것이라는 것을 증명하였다.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.29 no.1
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• pp.27-44
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• 2003

전반적인 피부세포의 생리적 지능 자하로 인한 자연노화와 더불어, 여러 피부 스트레스 요인들이 다양하게 작용하여 발생하는 외인성노화를 방지하거나, 개선할 수 있는 항노화소재로서의 개발 가능성을 알아보기 위해, 예로부터 항염활성이 있다고 알려진 oleanolic acid(OA)를 포함한 ursolic acid(UA), betulin, betulinic acid(BA) 등의 triterpenoids가 어떻게 피부에서의 항노화 활성을 나타내는지를 알아보았다. 시험 결과, OA는 자외선에 의한 각질형성세포에서의 PGE$_2$ 생성과 섬유아세포(NHF)에 의한 matrix metalloproteinase-1(MMP-1) 분비를 억제하였다. 그리고, NHF의 procollagen 생성을 촉진하였으며, 이런 procollagen 생성촉진활성이 in vivo에서도 발현되는 것을 무모생쥐의 실험을 통해서 확인하였다. 또한 OA는 각질세포의 증식과 분화를 촉진하여 표피세포로 하여금 세라마이므와 필라그린 생성을 증가시키도록 하는 작용도 있음을 보여주었다. 더불어 실험한 UA, betulin, BA 들은 비록, betulin, BA의 경우 세포 독성이 다른 물질 들에 비해 높았고, UA가 각질세포의 분화를 오히려 억제하는 양상을 보이기는 했지만, 대부분의 기능은 OA와 유사하였다. 피부세포보호작용과 진피 기질물질에 대한 작용, 그리고, 표피의 장벽기능과 보습기능에 대해 시험한 본 연구는, 식물성분인 triterpenoids가 피부를 위한 항노화소재로서의 개발 가능성이 있음을 확인하는 계기가 되었고, 그 중에서도 OA가 보다 우수한 항노화 소재가 될 수 있음을 시사하고 있다.

• Applied Microscopy
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• v.32 no.1
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• pp.45-55
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• 2002

The worm of Thelazia callipaeda Railliet et Henry, 1910 (The oriental eye worm) was frequently observed in the eyes of animal and human in Korea. But it did not clearly describe about the ultrastructural character on the sensory papillae and cuticular striation of the worm. This study was performed to investigate the ultrastructure and character on the cuticular surface of the worm that was extracted from the eyes of two patients in Korea University Medical Center, using the scanning electron microscopy. According to the mouth, 1/4, 1/2, 3/4 and tail portion of the worm, the size of cuticular striation on each portion was measured. The size of cuticular striation on the worm surface was $1.8{\mu}m$ in the mouth and tail portion, $4.0{\sim}4.5{\mu}m$ in the middle portion of the worm. On the scanning electron microscopy, the female worms were developed phasmids in the tail end and male worms were developed sensory papillae and external sexual organ on the tail end. The sensory papillae on the tail end were composed anterior ventral postcloacal papillae, middle ventral postcloacal papillae, subventral postcloacal papillae, and lateral papillae. According to the result in this study, it is considered that the character of the cuticular striation and the sensory papillae were able to accept as classifying key for the identification of species.