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Protective Effect of Glycyrrhiza glabra Extract on UV-induced Skin DNA Damage

감초추출물(Glycyrrhiza glabra Extract)의 피부에서의 DNA 손상 방지효과

  • 신재영 (LG 생활건강 기술연구원) ;
  • 강내규 (LG 생활건강 기술연구원)
  • Received : 2022.03.14
  • Accepted : 2022.03.24
  • Published : 2022.03.30

Abstract

Ultraviolet B (UVB) damages DNA residues in skin keratinocytes. In particular, the formation of cyclobutane pyrimidine dimers (CPD), a pyrimidine residue damage in DNA, is considered a representative indicator of skin photoaging. In this study, we confirmed defensive effect of Glycyrrhiza glabra (G. glabra) extract against UVB induced DNA damage. First of all, we confirmed UVB dependent amount of CPD formation in human keratinocyte cell line. UVB induced CPD was decreased by G. glabra extract by dose dependent manner. In addition, it was confirmed that the expression of mRNA of DNA damage recovery factors was increased by G. glabra extract. Consequently, through this study, it was possible to confirm the DNA protection effect of G. glabra extract in skin keratinocytes.

자외선인 ultraviolet B (UVB)는 피부각질세포의 DNA 잔기에 손상을 준다. 특히, DNA의 pyrimidine 잔기 손상인 cyclobutane pyrimidine dimers (CPD)의 형성은 피부 광노화의 대표적인 지표로 여겨진다. 본 연구에서는 피부 각질세포에서 UVB에 의한 DNA 손상을 완화 시키는 소재로 감초추출물(Glycyrrhiza glabra extract, G. glabra extract)의 효능을 확인하였다. 먼저 피부각질세포에서 UVB 의존적으로 CPD형성이 증가하는 것을 확인하였다. 이후 감초추출물에 의해 UVB 유발 CPD 형성이 유의하게 줄어드는 것을 확인할 수 있었다. 추가로 DNA 손상회복 인자의 mRNA 발현이 감초추출물에 의해 증가하는 것도 확인하였다. 결론적으로 본 연구를 통해 감초추출물의 피부각질세포에서의 DNA 보호 효과를 확인할 수 있었다.

Keywords

References

  1. C. Nishigori, D. B. Yarosh, S. E. Ullrich, A. A. Vink, C. D. Bucana, L. Roza, and M. L. Kripke, Evidence that DNA damage triggers interleukin 10 cytokine production in UV-irradiated murine keratinocytes, Proc. Natl. Acad. Sci. U S A., 93(19), 10354 (1996). https://doi.org/10.1073/pnas.93.19.10354
  2. E. Sage, Distribution and repair of photolesions in DNA: genetic consequences and the role of sequence context, Photochem Photobiol, 57(1), 163(1993). https://doi.org/10.1111/j.1751-1097.1993.tb02273.x
  3. G. T. Bowden, Prevention of non-melanoma skin cancer by targeting ultraviolet-B-light signalling, Nature Reviews Cancer, 4(1), 23 (2004). https://doi.org/10.1038/nrc1253
  4. M. Hochberg, R. Kohen, and C. D. Enk, Role of antioxidants in prevention of pyrimidine dimer formation in UVB irradiated human HaCaT keratinocytes, Biomed Pharmacother., 60(5), 233 (2006). https://doi.org/10.1016/j.biopha.2006.04.008
  5. N. S Jenny, Inflammation in aging: cause, effect, or both?, Discovery Medicine, 13(73), 451 (2012).
  6. I. M. Hadshiew, M. S. Eller, and B. Gilchrest, Skin aging and photoaging: the role of DNA damage and repair, Am J Contact Dermat., 11(1), 19 (2000). https://doi.org/10.1016/S1046-199X(00)90028-9
  7. S. Mouret, C. Baudouin, M. Charveron, A. Favier, J. Cadet, and T. Douki, Cyclobutane pyrimidine dimers are predominant DNA lesions in whole human skin exposed to UVA radiation, PNAS, 103(37), 13765 (2006). https://doi.org/10.1073/pnas.0604213103
  8. R. E. Watson, N. K. Gibbs, C. E. Griffiths, and M. J. Sherratt, Damage to skin extracellular matrix induced by UV exposure, Antioxid Redox Signal, 21(7), 1063 (2013).
  9. S. Lavasani, G. Henriksson, M. Brant, A. Henriksson, M. Radulic, R. Manthorpe, and A. Bredberg, Abnormal DNA damage-inducible protein in cells from Sjogren's syndrome patients, Journal of Autoimmunity, 11(4), 363 (1998). https://doi.org/10.1006/jaut.1998.0211
  10. Q. Afnan, M. D. Adil, A. Nissar-Ul, A. R. Rafiq, H. F. Amir, P. Kaiser, V. K. Gupta, R. Vishwakarma, and S. A, Tasduq, Glycyrrhizic acid (GA), a triterpenoid saponin glycoside alleviates ultraviolet-B irradiation-induced photoaging in human dermal fibroblasts, Phytomedicine, 19(7), 658 (1998). https://doi.org/10.1016/j.phymed.2012.03.007
  11. S. Franceschelli, M. Pesce, I. Vinciguerra, A. Ferrone, G. Riccioni, P. Antonia, A. Grilli, M. Felaco, and L. Speranza, Licocalchone-C extracted from Glycyrrhiza glabra inhibits lipopolysaccharide-interferon-γ inflammation by improving antioxidant conditions and regulating inducible nitric oxide synthase expression, Molecules, 16(7), 5720 (2011). https://doi.org/10.3390/molecules16075720
  12. V. K. Gupta, A. Fatima, U. Faridi, A. S. Negi, K. Shanker, J.K. Kumar, N. Rahuja, S. Luqman, B. S. Sisodia, D. Saikia, M. P. Darokar, and S. P. S. Khanuja, Antimicrobial potential of Glycyrrhiza glabra roots, Journal of ethnopharmacology, 116(2), 377 (2008). https://doi.org/10.1016/j.jep.2007.11.037
  13. G. P. Pfeifer and A. Besaratinia, UV wavelength-dependent DNA damage and human non-melanoma and melanoma skin cancer, Photochem Photobiol Sci., 11(1), 90 (2011). https://doi.org/10.1039/C1PP05144J
  14. T. Hasegawa, S. Shimada, H. Ishida, and M. Nakashima, Chafuroside B, an Oolong tea polyphenol, ameliorates UVB-induced DNA damage and generation of photoimmunosuppression related mediators in human keratinocytes, PLOS one., 8(10), e77308 (2013). https://doi.org/10.1371/journal.pone.0077308
  15. F. R de Gr uijl, H. J van Kr anen, and L. H. F Mullenders, UV-induced DNA damage, repair, mutations and oncogenic pathways in skin cancer, Journal of Photochemistry and Photobiology B: Biology, 63(1), 19 (2001). https://doi.org/10.1016/S1011-1344(01)00199-3
  16. K. S. Oh, M. Bustin, S. J. Mazur, E. Appella, and K. H. Kraemer, UV-induced histone H2AX phosphorylation and DNA damage related proteins accumulate and persist in nucleotide excision repair-deficient XP-B cells, DNA Repair (Amst), 10(1), 5 (2011). https://doi.org/10.1016/j.dnarep.2010.09.004
  17. L. Li, X. Lu, C. A. Peterson, and R. J. Legerski, An interaction between the DNA repair factor XPA and replication protein A appears essential for nucleotide excision repair, Molecular and cellular biology., 15(10), 5396 (1995). https://doi.org/10.1128/MCB.15.10.5396
  18. J. A. Marteijn, H. Lans, W. Vermeulen, and J. H. J. Hoeijmakers, Understanding nucleotide excision repair and its roles in cancer and ageing, Nature Reviews Molecular Cell Biology, 15(7), 465 (2014). https://doi.org/10.1038/nrm3822