• Toxicological Research
• /
• v.14 no.3
• /
• pp.293-300
• /
• 1998

Previous studies have shown that the heterocycles including thiazoles are efficacious in inducing phase phase II metabolizing enzyme as well as certain cytochrome P450s and that the inductin of these matabolizing enzymes by the heterocyclic agents is highly associated with their hepatotoxicity. In the present study, the effects of benzylisothiazole (BIT), which has a isothiazole moiety, on the expression of microsomal epoxide hydrolase (mEH), major glutathione S-transerases and cytochrome P450s were studied in the rat liver in association with its hepatotoxicity. Treatment of rats with BIT(1.17 mmol/kg, 1~3d) resulted in substantial increases in the mEH. rGSTA2, rGSTA2, rGSTM1 and rGSTM2 mRNA levels, whereas rGSTA3 and rGSTA5 mRNA levels were increased to much lesser extents. A time-course study showed that the mRNA levels of mEH and rGSTs were greater at 24hr after treatment than those after 3 days of consecutive treatment. Relative changes in mEH and rGST mRNA levels were consistent with those in the proteins, as assessed by Western immunoblot analysis. Hepatic cytochrom P450 levels were monitored after BIT treatment under the assumption that metabolic activation of BIT may affect expression of the enzymes in conjunction with hepatotoxicity. Immunoblot analysis revealed that cytochrome P450 2B1/2 were 3-to 4-fold induced in rats teatd with BIT(1.17 mmol/kg/day.3days), whereas P450 1A2, 2C11 and 3A1/2 levels were decreased to 20~30% of those in unteatd rats. P450 2E1 was only slightly decreased by BIT. Thus, the levels of several cytochrome P450s were suppressed by BIT treatment. Rats treated with BIT at the dose of 1.17mmol/kg for 3 days exhibited extensive multifocal nodular necrosis with moderate to extensive diffuse liver cell degeneration. No notable toxicity was observed in the kidney. These results showed that BIT induces mEH and rGSTs in the liver with increases in the mRNA levels, whereas the agent significantly decreased major cytochrome P450s. The changes in the detoxifying enzymes might be associated with the necrotic liver after consecutive treatment.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.9 no.1
• /
• pp.26-33
• /
• 1989

This pot experiments were conducted to find out the optimal fertilization ratios of the N:S:P anions and K:Ca:Mg cations of major nutrients in an orchardgrass/ladino clover mixed sward. The optimum ratios in equivalent basis were computed by the Homes systematic variations technique. The soil samples were collected from the newly reclaimed soils, which are located on colluvial hilly area with a good consideration for the hilly pasture development. The results were summarized as follows; 1, the optimum fertilization ratios of major nutrients for the high yields by the forage species in a mixed sward were obtained (Table 4 in detail); N:S:P = 3:l: 1 and K:Ca:Mg = 1:l: 1 for grass and grass plus legume, and N:S:P = 1:6:43 and K:Ca:Mg = 1: 3: 1 for legume in general. 2. The yield increases from the systematic variations in this mixed sward were laid in following order; N > P >S-group and K >Mg >Ca-group for grass and grass plus legume, and P >S >N-group and Ca >Mg > K-group for legume. Especially, the yields were greatly increased at the N-group for grass, and at the Pand Ca-groups for legume. 3. Soil pH-value was descreased at the Sgroup, and somewhat increased at the Ca- and Mg-groups. The content of available $P_2O_5$, CEC and base saturation were greatest with the Ca-group. 4. At the N-group, the N-contents were highest and the P-contents were lowest in grass and grass plus legume, which resulted in the highest Ca/P ratio of 2.15 among the anion groups. Whereas the highest Ca/P ratio of 9.20 in legume was obtained at the Ca-group. Legume showed in general higher Ca/P ratio and lower K/(Ca+Mg) ratio than these in grass. 5 . There were differences in the effects of systematic variations of major nutrients on the dry matter yields and the mineral yields. The optimum fertilization ratios of anions and cations for the high mineral yields were obtained (Table E), which showed differences comparing with the ratios for the high dry matter yields. The antagonis between the cations K and Mg was known from the point of mineral yields of mixed forages.

• The Korean Journal of Zoology
• /
• v.19 no.1
• /
• pp.25-32
• /
• 1976

To observe the changes of mucosubstances of the mucous secreting cells, stomach tissues in frog tadpoles at each stage of metamorphosis were fixed in 10% buffered formalin at $4^{\circ}C$, embedded in paraffin, sectioned to 4 $\mu$m thickness and stained with periodic acid-Schiff(PAS) and alcian blue (AB) of pH 2.5 and pH 1.0. The results obtained were as follows: 1. The reactivities of the surface mucous cells, which exhibited strong PAS-positivity and weak alcianophilia at both pH 2.5 and 1.0, were not changed in metamorphosis stages and the intracellular contents of neutral mucosubstances in the surface mucous cells increased significantly in XXIV and XXV stages of metamorphosis. 2. In the foveolar mucous cells, which appear after metamorphosis XXI, the staining reactivities to PAS, AB of pH 2.5 and 1.0 were the same as that of surface mucous cells during metamorphosis and the alcianophilia were stronger at pH 1.0 than at pH 2.5. 3. THe mucous neck cells, which appear after metamorphosis XXIV, exhibited a strong PAS-positive reaction and weak alcianophilia at metamorphosis XXIV but at metamorphosis XXV weak reactivity to PAS and strong alcianophilia at pH 1.0.

• Horticultural Science & Technology
• /
• v.29 no.1
• /
• pp.23-28
• /
• 2011

Experiments were conducted to investigate the optimum concentration of the nutrient solution in strawberry 'Sulhyang' with hydroponics in relationship between root activity and nutrient concentrations. Nutrient solutions for strawberry, made by Yamazaki, were supplied EC 0.5, 1.0, 2.0 $dS{\cdot}m^{-1}$ during experiment period. Growth of shoot and root of strawberries grown in visible plastic pot was observed during experiment. Petiole length was longest in plants grown in EC 1.0 $dS{\cdot}m^{-1}$, followed by 2.0 and 0.5 $dS{\cdot}m^{-1}$. Leaf width was longest in plants grown in EC 1.0 $dS{\cdot}m^{-1}$, followed by 0.5 and 2.0 $dS{\cdot}m^{-1}$. Fruit length, fruit diameter, fruit weight and yield were higher in EC 0.5 and 1.0 $dS{\cdot}m^{-1}$ than 2.0 $dS{\cdot}m^{-1}$ treatment but, soluble solids of the fruit did not show statistical differences among treatments. Shoot dry weight was heaviest in EC 1.0 $dS{\cdot}m^{-1}$, followed by 0.5 and 2.0 $dS{\cdot}m^{-1}$. Root dry weight was heavier in EC 0.5 and 1.0 $dS{\cdot}m^{-1}$ but significantly light in 2.0 $dS{\cdot}m^{-1}$. pH of the drainage solution was elevated in low nutrient concentration and lowered in high concentration. Also root activity was high in low nutrient concentration and low in high concentration. As a result, the optimum EC for strawberry 'Sulhyang' was EC 1.0 $dS{\cdot}m^{-1}$ in this experiment. It was confirmed that there was high relationship between root activity and pH of drainage solution. This result will be utilized as an indicator for strawberry hydroponics.

• Microbiology and Biotechnology Letters
• /
• v.36 no.1
• /
• pp.28-33
• /
• 2008

The expression vector (pWHM3-TR1R2) for sprT gene encoding Streptomyces griseus trypsin (SGT) followed by two regulatory genes, sgtR1 and sgtR2, was introduced into Streptomyces lividans TK24 and Streptomyces griseus IFO 13350. Various media with different compositions were used to maximize the productivity of SGT in the recombinant trains. he SGT productivity was best when the transformant of S. lividans TK24 was cultivated in R2YE medium (0.74 unit/mL) at 5 days of cultivation. C5/L (0.66 unit/mL) medium also gave a good productivity, but Livid (0.08 unit/mL) and NDSK (0.06 unit/mL) yielded poor productivities. S. griseus IFO 13350/pWHM3-TR1R2 produced SGT by 1.518 unit/mL (C5/L), 1.284unit/mL (R2YE),0.932 unit/mL (NDSK), and 0.295 unit/mL (Livid) at 7 days of cultivation, which was much higher than those from S. lividans TK24/TR1R2. The SGT protein was purified from the culture broth of S. griseus IFO 13350/pWHM3-TR1R2 in C5/L to homogeneity via ammonium sulfate fractionation, and CM-sepharose and SP-sepharose column chromatographies. The specific activity of purified SGT was 69,252 unit/mg, and the final purification fold and recovery yield were 6.5 and 1.4%, respectively.

• Kyungpook Mathematical Journal
• /
• v.59 no.1
• /
• pp.23-34
• /
• 2019

In this paper, we first define generalizations of Pell and Pell-Lucas sequences by the recurrence relations $$p_n=2ap_{n-1}+(b-a^2)p_{n-2}\;and\;q_n=2aq_{n-1}+(b-a^2)q_{n-2}$$ with initial conditions $p_0=0$, $p_1=1$, and $p_0=2$, $p_1=2a$, respectively. We give generating functions and Binet's formulas for these sequences. Also, we obtain some identities of these sequences.

• Journal of the Institute of Electronics Engineers of Korea SD
• /
• v.44 no.8
• /
• pp.22-29
• /
• 2007

A 2.5Gb/s 2:1 multiplexer(MUX) IC using $0.18{\mu}m$ CMOS was designed and fabricated. Inductive peaking technology was used to improve the performance. On-chip micro spiral inductor was designed to maximize the inductive peaking effect without increasing the chip area much. The designed 4.7 nH micro-spiral inductor was $20\times20{\mu}m2$ in size. 2:1 MUX with and without micro spiral inductors were compared. The rise and fall time was improved more than 23% and 3% respectively using the micro spiral inductors for 1.25Gb/s signal. For 2.5 Gb/s signal, fall and rise time was improved 5.3% and 3.5% respectively. It consumed 61mW and voltage output swing was 1$180mV_{p-p}$ at 2.5Gb/s.

• Communications for Statistical Applications and Methods
• /
• v.17 no.4
• /
• pp.507-513
• /
• 2010

Let {$X_i,-{\infty}$ < 1 < $\infty$} be a doubly infinite sequence of identically distributed and negatively associated random variables with mean zero and finite variance and {$a_i,\;-{\infty}$ < i < ${\infty}$} be an absolutely summable sequence of real numbers. Define a moving average process as $Y_n={\sum}_{i=-\infty}^{\infty}a_{i+n}X_i$, n $\geq$ 1 and $S_n=Y_1+{\cdots}+Y_n$. In this paper we prove that E|$X_1$|$^rh$($|X_1|^p$) < $\infty$ implies ${\sum}_{n=1}^{\infty}n^{r/p-2-q/p}h(n)E{max_{1{\leq}k{\leq}n}|S_k|-{\epsilon}n^{1/p}}{_+^q}<{\infty}$ and ${\sum}_{n=1}^{\infty}n^{r/p-2}h(n)E{sup_{k{\leq}n}|k^{-1/p}S_k|-{\epsilon}}{_+^q}<{\infty}$ for all ${\epsilon}$ > 0 and all q > 0, where h(x) > 0 (x > 0) is a slowly varying function, 1 ${\leq}$ p < 2 and r > 1 + p/2.

• Journal of applied mathematics & informatics
• /
• v.27 no.5_6
• /
• pp.1211-1220
• /
• 2009

In this paper, we consider the multipoint boundary value problem for the one-dimensional p-Laplacian $({\phi}_p(u'))'$(t)+q(t)f(t,u(t),u'(t))=0, t $\in$ (0, 1), subject to the boundary conditions: $u(0)=\sum\limits_{i=1}^{n-2}{\alpha}_iu({\xi}_i),\;u(1)=\sum\limits_{i=1}^{n-2}{\beta}_iu({\xi}_i)$ where $\phi_p$(s) = $|s|^{n-2}s$, p > 1, $\xi_i$ $\in$ (0, 1) with 0 < $\xi_1$ < $\xi_2$ < $\cdots$ < $\xi{n-2}$ < 1 and ${\alpha}_i,\beta_i{\in}[0,1)$, 0< $\sum{\array}{{n=2}\\{i=1}}{\alpha}_i,\sum{\array}{{n=2}\\{i=1}}{\beta}_i$<1. Using a fixed point theorem due to Bai and Ge, we study the existence of at least three positive solutions to the above boundary value problem. The important point is that the nonlinear term f explicitly involves a first-order derivative.

• Journal of Microbiology and Biotechnology
• /
• v.31 no.7
• /
• pp.921-932
• /
• 2021

LINC01272 is a long non-coding RNA (lncRNA) that has been considered as a biomarker for many diseases including lung squamous cell carcinoma. Here, we investigated the function and mechanism of LINC01272 on lung cancer (LC). The differential expression of LINC01272 in LC and normal samples was analyzed by GEPIA based on the data from TCGA-LUAD database, as survival prognosis was analyzed through Kaplan-Meier Plotter. LINC01272 overexpression plasmid and miR-7-5p mimic were transfected into A549 and PC-9 cells. LINC01272, miR-7-5p and cardiolipin synthase 1 (CRLS1) mRNA expression was measured by quantitative reverse transcription-polymerase chain reaction. Cell viability was detected through MTT assay. Cell multiplication was evaluated by cell formation assay. Cell apoptosis was assessed through flow cytometry assay. Through bioinformatics, the target miRNA of LINC01272 and downstream genes of miR-7-5p were predicted. The targeting relationship was tested by dual luciferase reporter analysis. CRLS1, B-cell lymphoma-2 (Bcl-2), BCL2-associated X (Bax) and cleaved caspase-3 protein levels were detected through western blot. LINC01272 was downregulated in LC and low LINC01272 expression had poor prognosis. In A549 and PC-9 cells, LINC01272 inhibited cell viability and multiplication and induced apoptosis. LINC01272 negatively regulated miR-7-5p and CRLS1 was a target of miR-7-5p. MiR-7-5p reversed the effect of LINC01272 on viability, multiplication, apoptosis and expression of miR-7-5p and CRLS1 as well as apoptosis-related factors (Bcl-2, Bax and cleaved caspase-3). LINC01272 suppressed cell multiplication and induced apoptosis via regulating the miR-7-5p/CRLS1 axis in LC.