• Title/Summary/Keyword: $L_1-B_4$

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Establishment of Sample Preparation Method to Enhance Recovery of Food-borne Pathogens from Produce (농산물 중 식중독세균 검출을 위한 전처리법 확립)

  • Kim, Se-Ri;Choi, Song-Yi;Seo, Min-Kyoung;Lee, Ji-Young;Kim, Won-Il;Yoon, Yohan;Ryu, Kyoung Yul;Yun, Jong-Cul;Kim, Byung-Seok
    • Journal of Food Hygiene and Safety
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    • v.28 no.3
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    • pp.279-285
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    • 2013
  • To establish sample preparation method for detection of food-borne pathogens from lettuce, perilla leaves, cucumber, pepper, and cherry tomato, the influences of diluent composition, processing time, and proportion of diluent to sample were examined. Each produce was inoculated with 6.0 log $CFU/cm^2$ of Escherichia coli O157:H7, Salmonella Typhimurium, Listeria monocytogenes, Staphylococcus aureus, and Bacillus cereus. Each produce was treated with 0.1% peptone water, and D/E neutralizing broth. Processing time of produce was 30, 60, 90, and 120s, and the proportion of diluent to sample was 2 : 1, 4 : 1, 9 : 1, and 19 : 1. The number of bacteria after treatment of D/E neutralizing broth was higher than that of 0.1% peptone water (P<0.05). In cherry tomato, the population of S. typhimurium recovered from treated with D/E broth was higher than that recovered from treated with 0.1% peptone water by 1.05 log $CFU/cm^2$ (P<0.05). No difference in numbers of pathogens was observed in processing time. Optimum proportion of diluent to perilla leaf, iceberg lettuce, cucumber, green pepper, and tomato was 9 : 1, 4 : 1, 2 : 1, 2 : 1, and 2 : 1, respectively. These data suggest that D/E neutralizing broth should be recommend as diluent, and the diluent volume applied to produce should be determined in proportion to produce surface area per weight (g).

A Study on Effect of B/L's Exemption Clauses Relating to the Governing Law of English Law (영국법의 준거법과 관련한 선하증권 면책약관의 효력에 관한 연구)

  • Han, Nak-Hyun;Jung, Jun-Sik
    • Journal of Korea Port Economic Association
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    • v.22 no.4
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    • pp.1-17
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    • 2006
  • In the Bill of Lading of The Irbenskiy Proliv is not subject to the Hague-Visby Rules in accordance with paragraphs (A) and/or (E) of cl.1 or to the Hague Rules in accordance with paragraphs (B) and/or (D) of cl.1. The Irbenskiy Proliv is very rare case that is effective to exempt the carrier as literal words of Bill of Lading. The action concerns cargoes of perishable goods shipped from Brazil to Japan, under Bills of Lading each of which contained an extensive carrier's exemption clause. A preliminary issue was ordered to be determined on the question whether c1.4 is effective to exempt the ralliers from any potential liability for the claims in this case. The court held that there is no reason to reject c1.4 as part of each of the contracts contained in or evidenced by the bills of lading; and it protects the carrier where damage to the goods shipped results from such causes. It is therefore effective to exempt the carriers from any potential liability for those claims.

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Development of KD- Propeller Series using a New Blade Section (새로운 날개단면을 이용한 KD-프로펠러 씨리즈 개발)

  • J.T. Lee;M.C. Kim;J.W. Ahn;H.C. Kim
    • Journal of the Society of Naval Architects of Korea
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    • v.28 no.2
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    • pp.52-68
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    • 1991
  • A new propeller series is developed using the newly developed blade section(KH18 section) which behaves better cavitation characteristics and higher lift-drag ratio at wide range of angle-of-attack. The pitch and camber distributions are disigned in order to have the same radial and chordwise loading distribution with the selected circumferentially averaged wake input. Since the geometries of the series propeller, such as chord length, thickness, skew and rate distribations, are selected by regression of the recent full scale propeller geometric data, the performance prediction of a propeller at preliminary design stage can be mure realistic. Number of blades of the series propellers is 4 and the expanded blade area ratios are 0.3, 0.45, 0.6 and 0.75. Mean pitch ratios are selected as 0.5, 0.65, 0.8, 0.75 and 1.1 for each expanded area ratio. The new propeller series is composed of 20 propellers and is named as KD(KRISO-DAEWOO) propeller series. Propeller open water tests are performed at the experimental towing tank, and the cavitation observation tests and fluctuating pressure measurements are carried out at the cavitation tunnel of KRISO. $B_{P}-\delta$ curves, which can be used to select the optimum propeller diameter at the preliminary design stage, are derived from a regression analysis of the propeller often water test results. The KD-cavitation chart is derived from the cavitation observation test results by choosing the local maximum lift coefficient and the local cavitation number as parameters. The caviy extent of a propeller can be predicted more accurately by using the KD-cavitation chart at a preliminary design stage, since it is derived from the results of the cavitation observation tests in the selected ship's wake, whereas the existing cavitation charts, such as the Burrill's cavitation chart, are derived from the test results in uniform flow.

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Growth Characteristics of Tomatoes Grafted with Different Rootstocks Grown in Soil during Winter Season (대목 종류에 따른 저온기 토경재배에서의 토마토 생육 특성 분석)

  • Lee, Hyewon;Lee, Jun Gu;Cho, Myeong Cheoul;Hwang, Indeok;Hong, Kue Hyon;Kwon, Deok Ho;Ahn, Yul Kyun
    • Journal of Bio-Environment Control
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    • v.31 no.3
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    • pp.194-203
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    • 2022
  • Cultivation of tomatoes in Korea grown in soil covers 89% of the total area for tomato cultivation. Tomatoes grown in soil often encounter various environment stresses including not only salt stress and soil-borne diseases but also cold stress in the winter season. This study was conducted to comparatively analyze the performance of rootstocks with cold stress by measuring the growth, yield, and photosynthetic efficiency in tomatoes grown in soil. The rootstocks were used 'Powerguard', 'IT173773', and '20LM' for the domestic rootstock cultivars and 'B-blocking' for a control cultivar. The tomato cultivar 'Red250' was used as the scion and the non-grafted tomatoes. Stem diameter, flowering position, leaf length, and leaf width were investigated for the growth parameters. The stem diameter of the non-grafted tomatoes decreased by 15% compared to the grafted tomatoes at 80 days after transplanting when exposed to low temperatures of 9-14℃ for 14 days. The leaf length and width of the non-grafted tomatoes were the lowest with 42.4 cm and 41.8 cm at 80 days after transplanting. The total yield per plant was the highest in tomato plants grafted on 'Powerguard' with 1,615 g and lowest in non-grafted tomatoes with 1,299 g. As the result of measuring the chlorophyll fluorescence parameters, PIABS and DI0/RC, which mean the performance index and dissipated energy flux, 'Powerguard' was the highest with 3.73 in PIABS and the lowest with 0.34 in DI0/RC, whereas non-grafted tomatoes was the lowest with 2.62 in PIABS and the highest with 0.41 in DI0/RC at 80 days after transplanting. The stem diameter has positive correlation with PIABS, while it has negative correlation with DI0/RC. The results indicate that can be analyzed by chlorophyll fluorescence parameters can be used for analyzing the differences in the growth of tomato plants grafted on different rootstocks when exposed to cold stress.

Effects of Packaging and Storage Temperature on Quality during Storage of Mungbean Sprouts (숙주나물의 저장 중 품질에 미치는 포장 및 저장온도의 영향)

  • Cho Sook-Hyun;Lee Sang-Dae;Choi Yong-Jo;Kim Nak-Goo;Kang Jin-Ho;Cho Sung-Hwan
    • Food Science and Preservation
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    • v.12 no.6
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    • pp.522-528
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    • 2005
  • Effects of packaging and storage temperature on the quality and shelf life of mungbean sprouts(vigna radiata (L.) Wilczek) were studied Mungbean sprouts were packaged in polypropylene films(PP) and oriented polypropylene films(OPP) with 200 g, 250 g, and 300 g and stored at $4^{\circ}C,\;8^{\circ}C$ and $12^{\circ}C$, respectively. The deterioration of quality of mungbean sprouts during storage was caused by wilting of hypocotyl, abscission of cotyledon and softening of tissue. Total weight loss never exceeded $1\%$ and no visible signs of shrivelling of mungbean sprouts were observed. At $4^{\circ}C,\;30{\mu}m$ of OPP film per 250 g mungbean sprouts provided the optimal atmosphere composition(i.e. $3\%\;\O_2\;and\;5\%\;CO_2$). A shelf life of 6 days was achieved with these conditions. Hardness of hypocotyl, when deterioration in freshness began, was about 1,027.2 g when considerably deteriorated Hunter b value was 13 in deteriorated hypocotyl, vs. 11 for hypocotyl of fresh mungbean sprouts was accelerated by fluctuating storage temperature by the increment of storage time. It also was found that the optimum shelf life period was estimated to be 6, 2 and 2 days for 4, 8 and $12^{\circ}C$, respectively.

A Whole Genome Association Study to Detect Single Nucleotide Polymorphisms for Carcass Traits in Hanwoo Populations

  • Lee, Y.-M.;Han, C.-M.;Li, Yi;Lee, J.-J.;Kim, L.H.;Kim, J.-H.;Kim, D.-I.;Lee, S.-S.;Park, B.-L.;Shin, H.-D.;Kim, K.-S.;Kim, N.-S.;Kim, Jong-Joo
    • Asian-Australasian Journal of Animal Sciences
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    • v.23 no.4
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    • pp.417-424
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    • 2010
  • The purpose of this study was to detect significant SNPs for carcass quality traits using DNA chips of high SNP density in Hanwoo populations. Carcass data of two hundred and eighty nine steers sired by 30 Korean proven sires were collected from two regions; the Hanwoo Improvement Center of National Agricultural Cooperative Federation in Seosan, Chungnam province and the commercial farms in Gyeongbuk province. The steers in Seosan were born between spring and fall of 2006 and those in Gyeonbuk between falls of 2004 and 2005. The former steers were slaughtered at approximately 24 months, while the latter steers were fed six months longer before slaughter. Among the 55,074 SNPs in the Illumina bovine 50K chip, a total of 32,756 available SNPs were selected for whole genome association study. After adjusting for the effects of sire, region and slaughter age, phenotypes were regressed on each SNP using a simple linear regression model. For the significance threshold, 0.1% point-wise p value from F distribution was used for each SNP test. Among the significant SNPs for a trait, the best set of SNP markers were selected using a stepwise regression procedure, and inclusion and exclusion of each SNP out of the model was determined at the p<0.001 level. A total of 118 SNPs were detected; 15, 20, 22, 28, 20, and 13 SNPs for final weight before slaughter, carcass weight, backfat thickness, weight index, longissimus dorsi muscle area, and marbling score, respectively. Among the significant SNPs, the best set of 44 SNPs was determined by stepwise regression procedures with 7, 9, 6, 9, 7, and 6 SNPs for the respective traits. Each set of SNPs per trait explained 20-40% of phenotypic variance. The number of detected SNPs per trait was not great in whole genome association tests, suggesting additional phenotype and genotype data are required to get more power to detect the trait-related SNPs with high accuracy for estimation of the SNP effect. These SNP markers could be applied to commercial Hanwoo populations via marker-assisted selection to verify the SNP effects and to improve genetic potentials in successive generations of the Hanwoo populations.

Quality Characteristics of Wet Noodles Combined with Cheongyang Hot Pepper (Capsicum annuum L.) Juice (청양고추 착즙액 첨가에 따른 생면의 품질특성)

  • Hwang, In-Guk;Kim, Ha-Yun;Hwang, Young;Jeong, Heon-Sang;Yoo, Seon-Mi
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.40 no.6
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    • pp.860-866
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    • 2011
  • We conducted this study to investigate the quality characteristics and antioxidant activity of noodles combined with Cheongyang hot pepper juice (CPJ). The noodles were evaluated for cooking properties (weight, volume, water absorption, and turbidity), Hunter's color values, texture characteristics, sensory characteristics, capsaicin, dihydrocapsaicin, polyphenol contents, and antioxidant activity. The cooked weight, volume, and turbidity of the cooked treated noodles were not significantly different from the cooked control noodles but water absorption decreased. The Hunter's color L value of the cooked noodles was not significantly different between treatment types, but there was a significantly higher b value with increasing concentrations of CPJ. The texture characteristics (hardness, adhesiveness, springiness, gumminess, and chewiness) of the cooked treated noodles were not significantly different from the cooked control noodles. A sensory evaluation indicated that cooked noodles treated with 2% CPJ were significantly (p<0.05) better than the cooked control noodles. Capsaicin, dihydrocapsaicin, and polyphenol contents, and antioxidant activity were significantly (p<0.05) increased with increasing concentrations of CPJ. In conclusion, CPJ could be used as an ingredient to increase the sensory and antioxidant properties of wheat flour noodles without affecting their quality characteristics.

Quality Characteristics of Jeolpyeon by Different Ratios of Lycil fructus Powder (구기자 가루를 첨가한 절편의 품질특성)

  • Lee, Mi-Young;Kim, Jong-Gun
    • Korean journal of food and cookery science
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    • v.23 no.6
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    • pp.818-823
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    • 2007
  • The purpose of this study was to investigate the effects of adding various concentrations of Lycil fructus powder on the quality characteristics of Jeolpyeon. The Lycil fructus powder was added to rice powder at ratios of 3, 6, 9 and l2%(w/w). The moisture contents of the Lycil fructus Jeolpyeon samples ranged from $46.3{\sim}48.9%$. For the color values the L-value decreased and the a-value and b-values incresaed with increasing amounts of Lycil fructus powder. In the textural analysis hardness was highest for the 3% Lycil fructus powder addition, however springiness and cohesiveness did not difference significantly by the addition levels. Finally the results of the sensory evaluation showed the Jeolpyeon containing 6% added with Lycil fructus powder was superior for overall preference, color, flavor, softness, chewiness and after-taste.

Seasonal Variations in Soil Solution Chemistry under Larix leptolepis, Pinus Koraiensis, and Quercus mongolica stands (일본잎갈나무, 잣나무, 신갈나무 임분 토양수 성질의 계절적 변이)

  • Ryu, Soung-Ryoul;Son, Yo-Whan;Joo, Yeong-Teuk;Jin, Hyun-O;Oh, Jong-Min;Jung, Duk-Young
    • Korean Journal of Environmental Agriculture
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    • v.19 no.1
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    • pp.75-79
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    • 2000
  • To investigate seasonal variations of soil solution chemistry, samples were collected from zero tension lysimeters in O, A, and B horizons of 26-year-old Larix leptolepis. Pinus koraiensis and Quercus mongolica stands in Kwangju, Kyunggi Province from September, 1996 through June. 1999. Potassium, $Mg^{2+}$, $Cl^-$, and $No_3\;^-$ concentrations increased in October and November concurrent with inputs of fresh litterfall and twigs. Sodium, $Ca^{2+}$, $K^+$, and $Mg^{2+}$ concentrations increased in March and April concurrent with the yellow sand effect. Potassium concentration showed the highest variation among ions, and $Cl^-$ concentration showed positive correlations with $K^+$ and $Mg^{2+}$ concentrations. Soil solution pH decreased while $Al^{3+}$ and $K^+$ concentrations increased during the study period. If our data reflects long term trends. then $Al^{3+}$ concentration in the O horizon will reach the toxic level (0.180 meq/l) within 10-20 years depending on species.

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Relationships between osteoporosis, alveolar bone density and periodontal disease in postmenopausal women (골다공증과 악골의 골밀도 및 치주 질환과의 상관 관계)

  • Han, E.Y.;Rhyu, I.C.;Lee, Y.M.;Ku, Y.;Han, S.B.;Choi, S.M.;Shin, J.Y.;Yang, S.M.;Chung, C.P.
    • Journal of Periodontal and Implant Science
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    • v.31 no.3
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    • pp.565-571
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    • 2001
  • The purpose of this study is to determine if a relationship exists among osteoporosis, alveolar bone density and periodontal disease in postmenopausal osteoporotic women and postmenopausal healthy women. Twenty-two women were evaluated for this study. They were attending the postmenopausal clinic, Seoul National University Hospital and generally healthy except osteoporosis. They had experienced menopause not less than one year when we began to examine them. Bone densities of lumbar area(L2-L4) was determined by DEXA(LUNAR-expert Co,. U.S.A). We diagnosed osteoporosis when T-score was below -2.5 and healthy state when T-score was over -1. Osteoporotic(10 female), not hormone-treated group and healthy control group(12 female) were asked for their age, menopausal age, menopausal period and the number of remaining teeth and examined clinically for plaque index(PI), gingival index(GI), clinical attachment loss(CAL) on their 6 Ramfjord index teeth. Intraoral radiographs were taken in maxillary anterior zone. All films were equally exposed and developed. Each films was digitized and analysed using image processing software, Scion image. Alveolar bone regions of interest were selected and Intensity of each pixel was quantized in the array ranging from 0(white) to 255(black). The two groups were comparable with respect age, menopausal age, menopausal period and number of remaining teeth. The osteoporotic women had significantly lower alveolar bone density than controls in maxilla. But no significant difference was found with respect clinical attachment loss, plaque index and gingival index. Supported by the Ministry of Public Health and Welfare, Korea (HMP-00-CH-10-0009).

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