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Characterization and refinement of enzyme of the gene encoding catechol 1,2-dioxygenase from Phenol-degrading, Rhodococcus sp.

  • 이희정;박근태;박재림;이상준
    • 한국환경과학회:학술대회논문집
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    • 한국환경과학회 2002년도 봄 학술발표대회 발표논문집
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    • pp.209-212
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    • 2002
  • 본 연구는 방향족 화합물질 중 페놀폐수에 대한 생물학적 처리를 위해 본 실험실에서 분리한 페놀분해능이 우수한 Rhodococrus sp. EL-GT를 이용하여 catechol 분해 catechol 1,2-dioxygenase 분해활성을 측정하였고, 이것이 ortho-pathway임을 확인할 수 있었다. 또한 다른 연구에서 보고된 Rhodococcus rhodochrous NCIMB 13259 균주의 catechol 1,2 dioxygenase를 기초로한 primer를 이용하여 PCR을 수행하였으며 이 분해 유전자의 cloning실험을 수행 중이다. 이들 실험을 통하여 Rhodococcus sp.의 페놀분해균의 유전적 구조 및 특성을 검토하고 밝혀지는 단백질 정보를 이용하여 방향족 화합물의 분해능이 보다 우수한 균주의 개발을 시도하고자 한다.

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실습선 새바다호의 선체진동에 관하여 (On the Hull Vibration of the Training Ship Sae-Ba-Da)

  • 박중희
    • 수산해양기술연구
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    • 제22권3호
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    • pp.23-28
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    • 1986
  • This paper describes on the measurement of the deck vibration produced by the main engine vibration of stern trawler MjS SAE-BA-DA (2,275GT, 3,600PS) while the ship is cruising and drifting. The obtained results are as follows; 1. The deck vibration level was the highest point at vertical line which pass main engine and the lowest point at vertical line which pass top bridge while the crusing. 2. The vibration source level of the main engine, screw shaft and screw propeller were respectively 110, 90 and 80% while the crusing. 3. The main deck vibration pressure level at the check points 2, 20, 30, 40, 60, 70, 80, 86m from the bow to stern was respectively 9, 8, 7, 10, 22, 45, 18, 23%. 4. The frequency distributions of the rr.ain engine, screw shaft, screw propeller vibration were from 3 Hz to 10 KHz, predominant frequency was 1 KHz, each vibration accelration the highest level were respectively 1. 3, 0.8, 0.5 $mm/s^2.$ 5. The predominant frequency distributions of the main deck, second deck, bridge deck and top bridge deck-s vibration were from 10 to 30 Hz, and each vibration accelration level were respectively 0.7, 0.05, 0.07, 0.04 $mm/s^2.$

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한국 근해에 있어서의 중층 트로올의 연구 - 1 . 전개판의 동작상태의 전개성능 - (Study On The Midwater Trawl Available in the Korean Waters - 1 . Attitude and Opening Opening Efficiency of Otter Board -)

  • 이병기
    • 수산해양기술연구
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    • 제22권4호
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    • pp.41-48
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    • 1986
  • To investigate the midwater trawl gear available for the Korean near sea trawlers, the authors carried out a field experiment on the attitude and the opening efficiency of the otter board with the Pusan 404 (160GT, 750ps), a training ship of National Fisheries University of Pusan. The experimental trawl gear was designed to be operable by the ship and the otter board was made of single iron plate with 12% camber ratio. The special-prepared potentiometric angle detector was used for determining the attitude, and the 50 KHz fish finder for the opening between the otter boards. The results obtained can be summarized as follows: 1. The angle of attack varied by moving the towing point on the towing plate of the otter board. It showed 33 to 36 degrees when the point was set at the outmost position, 25 to 31 degrees at the middle position and 19 to 30 degrees at the inmost position, with a decreasing tendency according to the increase of towing speed in each cases. 2. The heel of the otter board always occured inwards, increasing slightly according to the increase of towing speed. 3. The tilt of the otter board always occured outwards, increasing slightly according to the move of the towing point inwards, and decreasing slightly according to the increase of towing speed. 4. The opening between otter boards showed the largest value when the towing point was set at the outmost position.

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실습선 새바다호의 선체진동에 관하여 (On the Hull Vibration of the Training Ship Sae-Ba-Da)

  • 박중희
    • 어선
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    • 제29권
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    • pp.15-20
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    • 1986
  • This paper describes on the measurement of the deck vibration produced by the main engine vibration of stern trawler MIS SAE-BA-DA (2,275GT, 3,600PS) while the ship is cruising and drifting. The obtained results are as follows; 1. The deck vibration level was the highest point at vertical line which pass main engine and the lowest point at vertical line which pass top bridge while the crusing. 2. The vibration source level of the main engine, screw shaft and screw propeller were respectively 110, 90 and 80% while the crusing. 3. The main deck vibration pressure level at the check points 2, 20, 30, 40, 60, 70, 80, 86m from the bow to stern was respectively 9, 8, 7, 10, 22, 45, 18, 23%. 4. The frequency distributions of the main engine, screw shaft, screw propeller vibration were from 3Hz to 10KHz, predominant frequency was 1KHz, each vibration accelration the highest level were respectively 1.3, 0.8, 0.5mm/$S^2$. 5. The predominant frequency distributions of the main deck, second deck, bridge deck and top bridge deck's vibration were from 10 to 30Hz, and each vibration accelration level were respe¬ctively 0.7, 0.05, 0.07, 0.04mm/$S^2$.

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방사선치료 시 전자포털 영상장치를 이용한 잡음전력스펙트럼 수평 및 수직 측정비교 (Comparison of Horizontal and Vertical Noise Power Spectrum in Measurements by Using Various Electronic Portal Imaging Devices in Radiation Therapy)

  • 김기원;최관우;정회원;장서구;권경태;손순룡;손진현;민정환
    • 대한방사선기술학회지:방사선기술과학
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    • 제39권2호
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    • pp.171-176
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    • 2016
  • 영상화 품질향상은 방사선 치료영역과 진단영역의 영상화 검사와 같이 quality assurance (QA) 장비들의 특성평가에 매우 중요한 요소이다. 본 연구의 목적은 치료장비의 megavoltage X-ray 에너지를 사용하여 측정방법에 있어서 NPS에 대한 수평, 수직적인 방법에 대한 측정결과를 비교 평가하는 것이다. 잡음전력스펙트럼 평가 방법들은 국제 전기 규격 international electro-technical commission (IEC 62220-1) 기준을 사용하여 치료 영역에 적용되었다. 사용한 전자포털 영상장치(electronic portal imaging device, EPID)는 Siemens BEAMVIEW$^{PLUS}$, Elekta iViewGT 그리고 Varian Clinac$^R$ iX aS1000이었다. 데이터들로부터 각각의 주파수 값에 대응하는 노이즈 값들의 평균에 대해서, 각각의 주파수 별로 대응해서 표현하였으며, 수평 수직되어있는 주파수별 평균값의 노이즈값을 합산해서 정량적으로 장비마다 비교 평가하였다. 결과값은 Elekta iView에서 4가지 방법론에 의한 가로방향에서의 horizontal측정된 NPS값과 vertical 세로방향에서의 측정치는 비슷한 경향성에 따라서 horizontal과 vertical값은 3~5%정도의 차이가 있었으며, Siemens BEAMVIEW$^{PLUS}$, Varian Clinac$^R$ iX aS1000에서 horizontal측정된 NPS값과 vertical 측정된 NPS값은 편차의 차이가 전체값의 평균으로 했을 시 horizontal과 vertical값은 15%정도의 차이가 나타남을 보였다. 본 연구에서는 수평 수직되어있는 주파수별 평균값의 노이즈값을 합산해서 정량적으로 장비마다 비교 평가하였으며, 정량적인 평가방안을 제시하였다는 점을 둘 수 있다.

DNA-functionalized single-walled carbon nanotube-based sensor array for gas monitoring

  • Zhang, Wenjun;Liu, Yu;Wang, Ming. L
    • Smart Structures and Systems
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    • 제12권1호
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    • pp.73-95
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    • 2013
  • Nine deoxyribonucleic acid (DNA) sequences were used to functionalize single-walled carbon nanotube (SWNT) sensors to detect the trace amount of methanol, acetone, and HCl in vapor. DNA 24 Ma (24 randomly arranged nitrogenous bases with one amine at each end of it) decorated SWNT sensor and DNA 24 A (only adenine (A) base with a length of 24) decorated SWNT sensor have demonstrated the largest sensing responses towards acetone and HCl, respectively. On the other hand, for the DNA GT decorated SWNT sensors with different sequence lengths, the optimum DNA sequence length for acetone and HCl sensing is 32 and 8, separately. The detection of methanol, acetone, and HCl have identified that DNA functionalized SWNT sensors exhibit great selectivity, sensitivity, and repeatability with an accuracy of more than 90%. Further, a sensor array composed of SWNT functionalized with various DNA sequences was utilized to identify acetone and HCl through pattern recognition. The sensor array is a combination of four different DNA functionalized SWNT sensors and two bare SWNT sensors (work as reference). This wireless sensing system has enabled real-time gas monitoring and air quality assurance for safety and security.

Nucleotide Sequence of Rat Transketolase and Liver-Specific Pretranslational Activation During Postnatal Development

  • Kim, Sung-Min F.;Kim, Byung-Moon;Jeng, Jingjau;Soh, Yun-Jo;Bak, Choong-Il;Huh, Jae-Wook;Song, Byoung-J.
    • BMB Reports
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    • 제29권2호
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    • pp.146-150
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    • 1996
  • A 2.1 kb cDNA clone for rat transketolase was isolated from rat liver ${\lambda}gt11$ cDNA library and its sequence was determined. The predicted rat transketolase (655 amino acids with $M_r$ 71,186) is highly similar (92%) to that of the human enzyme except that it contains an extra 32 amino acids at its N-terminus. Although it is less similar (<27%) to transketolases from non-mammalian species, the functional motifs such as the catalytic sites and thiamine binding domain are well conserved in the rat enzyme. Southern blot analysis of genomic DNA verified that transketolase appears to be derived from a single gene. Immunoblot and Northern blot analyses suggested that hepatic transketolase was activated pretranslationally by a 2.1-fold while little change was observed in brain enzyme, indicating a tissue-specific pretranslational activation during postnatal development.

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Molecular Characterization of Rockbream (Oplegnathus fasciatus) Cytoskeletal β-actin Gene and Its 5'-Upstream Regulatory Region

  • Lee, Sang-Yoon;Kim, Ki-Hong;Nam, Yoon-Kwon
    • Fisheries and Aquatic Sciences
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    • 제12권2호
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    • pp.90-97
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    • 2009
  • The cytoskeletal $\beta$-actin gene and its 5'-upstream region were isolated and characterized in the rockbream (Oplegnathus fasciatus). Complementary DNA of the rockbream $\beta$-actin represented a 1,125 bp of an open reading frame encoding 375 amino acids, and the rockbream $\beta$-actin cDNA and deduced amino acid sequences were highly homologous to those of other vertebrate orthologs. At the genomic level, the $\beta$-actin gene also exhibited an organization typical of vertebrate cytoskeletal actin genes (2,159 bp composed of five translated exons interrupted by four introns) with a conserved GT/AG exon-intron splicing rule. The putative non-translated exon predicted in the rockbream $\beta$-actin gene was much more homologous with those of teleostean $\beta$-actin genes than those of mammals. The 5'-upstream regulatory region isolated by genome walking displayed conserved and essential elements such as TATA, CArG and CAAT boxes in its proximal part, while several other immune- or stress-related motifs such as those for NF-kappa B, USF, HNF, AP-1 and C/EBP were in the distal part. Semi-quantitative RT-PCR assay results demonstrated that the rockbream $\beta$-actin transcripts were ubiquitously but different-tially expressed across the tissues of juveniles.

T-NEIGHBORHOODS IN VARIOUS CLASSES OF ANALYTIC FUNCTIONS

  • Shams, Saeid;Ebadian, Ali;Sayadiazar, Mahta;Sokol, Janusz
    • 대한수학회보
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    • 제51권3호
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    • pp.659-666
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    • 2014
  • Let $\mathcal{A}$ be the class of analytic functions f in the open unit disk $\mathbb{U}$={z : ${\mid}z{\mid}$ < 1} with the normalization conditions $f(0)=f^{\prime}(0)-1=0$. If $f(z)=z+\sum_{n=2}^{\infty}a_nz^n$ and ${\delta}$ > 0 are given, then the $T_{\delta}$-neighborhood of the function f is defined as $$TN_{\delta}(f)\{g(z)=z+\sum_{n=2}^{\infty}b_nz^n{\in}\mathcal{A}:\sum_{n=2}^{\infty}T_n{\mid}a_n-b_n{\mid}{\leq}{\delta}\}$$, where $T=\{T_n\}_{n=2}^{\infty}$ is a sequence of positive numbers. In the present paper we investigate some problems concerning $T_{\delta}$-neighborhoods of function in various classes of analytic functions with $T=\{2^{-n}/n^2\}_{n=2}^{\infty}$. We also find bounds for $^{\delta}^*_T(A,B)$ defined by $$^{\delta}^*_T(A,B)=jnf\{{\delta}&gt;0:B{\subset}TN_{\delta}(f)\;for\;all\;f{\in}A\}$$ where A, B are given subsets of $\mathcal{A}$.

디지털 영상 시스템을 이용한 알루미늄 당량화상에 의한 골량 측정에 관한 연구 (A study on assessment of bone mass from aluminum-equivalent image by digital imaging system)

  • 김진수;최의환;김재덕
    • 치과방사선
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    • 제27권1호
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    • pp.87-97
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    • 1997
  • The purpose of this study was to evaluate the method for quantitative assessment of bone mass from aluminum-equivalent value of hydroxyapatite by using digital imaging system consisted of Power Macintosh 7200/120, 15-inch color monitor, and GT-9000 scanner with transparency unit. After aluminum-equivalent image made from correlation between aluminum thickness and grey scale, the accuracy of conversion to mass from aluminum-equivalent value was evaluated. Measured bone mass was compared with converted bone mass from aluminum-equivalent value of hydroxyapatite block by correlation formula between aluminum-equivalent value of hydroxyapatite block and hydroxyapatite mass. The results of this study were as follow; 1. Correlation between aluminum thickness and grey level for obtaining aluminum-equivalent image was high positively associated(r²=0.99). Converted masses from aluminum-equivalent value were very similar to measured masses. There was, statistically, no significant difference(P<0.05) between them 2. Correlation between hydroxyapatite aluminum-equivalent and hydroxyapatite mass was shown to linear relation (r²=0.95). 3. Converted masses from aluminum-equivalent value of 3 dry mandible segments were similar to measured masses. The difference between the exposure directions was not significantly different(P<0.05).

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