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http://dx.doi.org/10.5657/fas.2009.12.2.090

Molecular Characterization of Rockbream (Oplegnathus fasciatus) Cytoskeletal β-actin Gene and Its 5'-Upstream Regulatory Region  

Lee, Sang-Yoon (Department of Aquaculture, Pukyong National University)
Kim, Ki-Hong (Department of Aquatic Life Medicine, Pukyong National University)
Nam, Yoon-Kwon (Department of Aquaculture, Pukyong National University)
Publication Information
Fisheries and Aquatic Sciences / v.12, no.2, 2009 , pp. 90-97 More about this Journal
Abstract
The cytoskeletal $\beta$-actin gene and its 5'-upstream region were isolated and characterized in the rockbream (Oplegnathus fasciatus). Complementary DNA of the rockbream $\beta$-actin represented a 1,125 bp of an open reading frame encoding 375 amino acids, and the rockbream $\beta$-actin cDNA and deduced amino acid sequences were highly homologous to those of other vertebrate orthologs. At the genomic level, the $\beta$-actin gene also exhibited an organization typical of vertebrate cytoskeletal actin genes (2,159 bp composed of five translated exons interrupted by four introns) with a conserved GT/AG exon-intron splicing rule. The putative non-translated exon predicted in the rockbream $\beta$-actin gene was much more homologous with those of teleostean $\beta$-actin genes than those of mammals. The 5'-upstream regulatory region isolated by genome walking displayed conserved and essential elements such as TATA, CArG and CAAT boxes in its proximal part, while several other immune- or stress-related motifs such as those for NF-kappa B, USF, HNF, AP-1 and C/EBP were in the distal part. Semi-quantitative RT-PCR assay results demonstrated that the rockbream $\beta$-actin transcripts were ubiquitously but different-tially expressed across the tissues of juveniles.
Keywords
Rockbream (Oplegnathus fasciatus); $\beta$-actin; Gene structure; mRNA expression; Regulatory region;
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