• Journal of Plant Biology
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• 제15권2호
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• pp.7-19
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• 1972

The studies of density effect or the effect of population density on plant growth have been done on basis of dry matter production with Raphanus acanthiformis var. simoodaeguen, Brassica campestris var. Pekinensis f. namsounsokoombecheu, Oryza sativa f. kimmajae and O. sativa f. mangyeng grown in the various spacing. 1. In the early period of plant growth in dry weight was not different each other among varying densities, but as time advanced the plant grown vast space grew sufficiently compared with those of narrow one. 2. Iogarithmic relation between the growth of plant (W) and the density (P), log W-log P in the material plants, were approximated by two straight lines, one was horizontal line and another inclined: the former showed non-competition density and the latter competition density addition to these the point interlinking both lines were implied of the optimum density per unit land area at certain growth period. 3. The values of relatvie growth rate (RGR) and net assimilation rate (NAR) were decreased as increase in the density, while those of leaf area ratio (LAR) were rather increased in the same condition, with minor exception. From these results and relation between the productive structure and due to lack of the recieved light intensity owing to the mutal shading among the plants.

• 한국미생물·생명공학회지
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• 제30권2호
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• pp.162-166
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• 2002

제주도 서귀포의 해변에서 채집한 해양퇴적물로부터 Myxococcus속의 점액세균 JW154를 분리하여 그 대사산물로부터 강한 항균활성을 나타내는 두 개의 bithiazole계 화합물을 분리하고 각종 기기분석을 통하여 구조를 확인한 바, 이들은 각각 KR025와 melithiazole F로 보고된 화합물임을 밝혔다. 유묘 검정법으로 조사된 이 화합물의 주요 식물병원균에 대한 in vivo 항균활성은 토마토 역병과 밀 녹병 및 보리 흰가루병에 대해 높게 나타났는데, 20$\mu\textrm{g}$/m1 수준으로 처리하였을 때 80% 이상의 높은 방제가를 보였다.

• 대한화장품학회지
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• 제31권1호
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• pp.115-119
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• 2005

제주에서 자생하는 식물들의 미백활성을 B16F10 세포에서의 멜라닌 생성 억제, mushroom tyrosinase 활성 억제 실험을 통하여 확인하였다. 본 실험에서 우리는 개민들레 줄기, 까마중, 미국미역취, 돌외, 주목의 메탄을 추출물에서 B16F10 세포에서의 멜라닌 생성 저해 효과를 확인하였다. 그러나 이들의 tyrosinase 활성은 없었다.

• 한국자원식물학회지
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• 제34권6호
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• pp.510-516
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• 2021

Whitening agents derived from natural sources which do not have side effects are sought after. Schizophragma hydrangeoides is an edible plant that grows wild on Jeju Island. We aimed to determine whether S. hydrangeoides extracts show anti-melanogenic activity. Here, we found that 70% ethanol extracts of S. hydrangeoides leaf suppressed α-melanocyte-stimulating hormone-induced melanogenesis in B16F10 mouse melanoma cells. This activity of anti-melanogenesis in B16F10 cells were investigated by determining melanin content and tyrosinase activity, and by performing western blotting. The 70% ethanol extract downregulated tyrosinase and tyrosinase-related protein 1. In addition, the n-hexane fraction of S. hydrangeoides leaf (HFSH) exhibited significant anti-melanogenic activity among the various solvent fractions tested without reducing the viability of B16F10 cells. Taken together, these results indicate that extracts from S. hydrangeoides leaf can influence cellular processes via modulation of tyrosinase activity. Hence, S. hydrangeoides can be used as a whitening agent in the cosmetic industry and as a therapeutic agent for treating hyperpigmentation disorders in the clinic.

• The Plant Pathology Journal
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• 제26권4호
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• pp.409-416
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• 2010

Fusarium species, a large group of plant pathogens, potentially pose quarantine concerns worldwide. Here, we focus on the development of a method for detecting four Fusarium species in quarantined plants in Korea: F. solani f. sp. cucurbitae, F. stilboides, F. redolens, and F. semitectum var. majus. Species-specific primers were designed from the nucleotide sequences of either the translation elongation factor-1 alpha (TEF1) gene or RNA polymerase II subunit (RPB2) gene. Two different primer sets derived from TEF1, all specific to F. solani f. sp. cucurbitae, were able to differentiate the two races (1 and 2) of this species. A set of nested primers for each race was designed to confirm the PCR results. Similarly, two primer sets derived from RPB2 successfully amplified specific fragments from five F. stilboides isolates grouped within a single phylogenetic clade. A specific TEF1 primer set amplified a DNA fragment from only four of the 12 F. redolens strains examined, which were grouped within a single phylogenetic clade. All of the F. semitectum var. majus isolates could be specifically detected with a single RPB2 primer set. The specificity of the primer sets developed here was confirmed using a total of 130 Fusarium isolates.

• The Plant Pathology Journal
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• 제38권3호
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• pp.254-260
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• 2022

Fusarium incarnatum-equiseti species complex (FIESC) contain over 40 members. The primer pair Smibo1FM/Semi1RM on the RPB2 partial gene has been reported to be able to identify Fusarium semitectum. The F. fujikuroi species complex (FFSC) contains more than 50 members. The F. verticillioides as a member of this complex can be identified by using VER1/VER2 primer pair on the CaM partial gene. In this research, the Smibo1FM/Semi1RM can amplify F. sulawesiense, F. hainanense, F. bubalinum, and F. tanahbumbuense, members of FIESC at 424 bp. The VER1/VER2 can amplify F. verticillioides, F. andiyazi, and F. pseudocircinatum, members of FFSC at 578 bp. Polymerase chain reaction-restriction fragment length polymorphism by using the combination of three restriction enzymes EcoRV, MspI, and HpyAV can differentiate each species of FIESC used. The two restriction enzymes HpaII and NspI can distinguish each species of FFSC used. The proper identification process is required for pathogen control in the field in order to reduce crop yield loss.

• 식물분류학회지
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• 제37권4호
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• pp.545-563
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• 2007

2005년 11월부터 2006년 11월까지 총 4회에 걸쳐 독도의 유관속식물상을 조사하였다. 독도에서는 26과 44속 46종 1아종 1변종으로 총 48분류군이 확인되었는데, 이는 양치식물 1분류군, 쌍자엽식물 36분류군, 단자엽식물 11분류군으로 이루어져 있다. 식물구계학적 특정식물로는 V등급종인 초종용(Orobanche coerulescens Stephan)과 IV등급종인 왕호장근[Fallopia sachalinensis (F.Schmidt) RonseDecr.], 섬괴불나무(Lonicera insularis Nakai), 섬초롱꽃(Campanula takesimana Nakai), 왕김의털(Festuca rubra L.) 등 총 13분류군이 확인되었다. 식재된 종으로는 곰솔(Pinus thunbergii Parl.), 동백나무(Camellia japonica L,) 보리장나무(Elaeagnus glabra Thunb), 무궁화(Hibiscus syriacus L.) 등이 있다. 분류학적으로 실체가 의심되는 종(species)으로는 가는명아주(Chenopodium virgatum Thunb.), 돌피[Echinochlca crusgalli (L.) P.Beauv.]로써 각각 흰명아주(Chenopodium album L,), 물피[Echinochloa crusgalli var. oryzicola (Vasinger) Ohwi]와 구분되는 형질을 공유하거나 불분명하여 동정에 있어서 혼란이 야기된다. 종유입 방법별로는 풍산포(anemochore) 31분류군, 동물산포(zoochore) 8분류군, 해류산포 (hydrochore) 2분류군인 것으로 판단되며, 이외의 종들은 인위적 수단에 의하여 유입된 것으로 보인다.

• 식물병연구
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• 제23권4호
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• pp.295-305
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• 2017

This study aimed to investigate the plant growth-promoting and biocontrol potential of a grass culture with Paenibacillus ehimensis KWN8 on tomato. For this experiment, treatments of a chemical fertilizer (F), a bacterial grass culture (G), a 1/3 volume of G plus 2/3 F (GF), and F plus a synthetic fungicide (FSf) were applied to tomato leaves and roots. The result showed that the severity of Alternaria solani and Botrytis cinerea symptoms were significantly reduced after the application of the bacterial grass culture (G and GF) and FSf. In addition, root mortality in G and GF was lower compared to F. Tomato plants treated with G or GF had better vegetative growth and yield compared to F. Application of G affected the fungal and bacterial populations in the soil. In conclusion, treatment with a bacterial grass culture decreased disease severity and increased tomato growth parameters. However, there were no statistically significant correlations between disease occurrence and tomato yields. This experiment presents the possibility to manage diseases of tomato in an environmentally friendly manner and to also increase the yield of tomato by using a grass culture broth containing P. ehimensis KWN38.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2018년도 추계학술대회
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• pp.95-95
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• 2018

In this study, we investigated the effect of branch extracts from Vaccinium oldhamii (VOB) on melanin synthesis in B16F10 cells. VOB promoted melanin production in absence or presence of ${\alpha}$-melanocyte-stimulating hormone (${\alpha}-MSH$) in B16F10 cells. However, VOB did not affect the expression of tyrosinase and TRP-1 associated with melanin synthesis at the mRNA and protein level in B16F10. But, VOB decreased TRP-2 protein level and induced tyrosinase activation in B16F10 cells. Inhibition of tyrosinase activity and tyrosinase knockdown attenuated VOB-mediated melanin synthesis. In conclusion, it is thought that VOB may stimulate melanin synthesis through activating tyrosinase activity.

• 식물분류학회지
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• 제52권4호
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• pp.226-234
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• 2022

The study aims are to examine the characteristics of artificial and natural hybrids between Viola albida var. albida (= albida, from below) and V. albida var. chaerophylloides (= chaerophylloides, from below), and to confirm if hybrids could be fertile and make populations in their native habitats. The 1st filial (= F₁, from below) leaf shape produced by artificial crossing between albida and chaerophylloides was the same as that of V. albida var. takahashii (= takahashii, from below), and F₁ bore also both chasmogamous and cleistogamous flowers. F₁ seed number was 9.6 per cleistogamous pods, which was remarkably less than the average of 38.2 for albida and chaerophylloides, but the germination rate was all similar. The leaf type of self-crossed 2nd offsprings (= F₂, from below) showed all leaf types found in the Viola albida complex, but the ratio of chaerophylloides leaf type was relatively low. Individuals whose F₂ leaf type was restored to albida produced an average of 31.4 seeds per capsule, meaning that fertility was restored. On the other hand, individuals of F₂ takahashii leaf type come to fruition a low average of 10.4 seeds per capsule, which is similar to that of takahashii. The results of crossbreeding experiment, where is their native habitats, were similar to that of laboratory. Both albida and chaerophylloids in Mt. Bulmyeong distribute extensively, but takahashii make a small population only in places where albida and chaerophylloides grow together. Summarizing the above results is suggesting that the speciation of takahashii was done by hybrid between albida and chaerophylloides, and these have been maintained with relatively small population by cleistogamous capsules.