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Speciation of Viola albida var. takahashii based on both artificial and natural hybridization between V. albida var. albida and V. albida var. chaerophylloides

  • Songhee, AHN (Herb and Wild Plants Experiment Station, Jeollabuk-do Agricultural Research & Extension Services) ;
  • Sung Soo, WHANG (Division of Science Education, Jeonbuk National University)
  • Received : 2022.07.09
  • Accepted : 2022.12.28
  • Published : 2022.12.31

Abstract

The study aims are to examine the characteristics of artificial and natural hybrids between Viola albida var. albida (= albida, from below) and V. albida var. chaerophylloides (= chaerophylloides, from below), and to confirm if hybrids could be fertile and make populations in their native habitats. The 1st filial (= F1, from below) leaf shape produced by artificial crossing between albida and chaerophylloides was the same as that of V. albida var. takahashii (= takahashii, from below), and F1 bore also both chasmogamous and cleistogamous flowers. F1 seed number was 9.6 per cleistogamous pods, which was remarkably less than the average of 38.2 for albida and chaerophylloides, but the germination rate was all similar. The leaf type of self-crossed 2nd offsprings (= F2, from below) showed all leaf types found in the Viola albida complex, but the ratio of chaerophylloides leaf type was relatively low. Individuals whose F2 leaf type was restored to albida produced an average of 31.4 seeds per capsule, meaning that fertility was restored. On the other hand, individuals of F2 takahashii leaf type come to fruition a low average of 10.4 seeds per capsule, which is similar to that of takahashii. The results of crossbreeding experiment, where is their native habitats, were similar to that of laboratory. Both albida and chaerophylloids in Mt. Bulmyeong distribute extensively, but takahashii make a small population only in places where albida and chaerophylloides grow together. Summarizing the above results is suggesting that the speciation of takahashii was done by hybrid between albida and chaerophylloides, and these have been maintained with relatively small population by cleistogamous capsules.

Keywords

Acknowledgement

This study was partly supported by a research grant of Jeonbuk National University (2022-2023).

References

  1. Ahn, S. H. 2015. A speciation study of Viola albida complex based on artificial hybridization. MS thesis, Jeonbuk National University, Jeonju, Korea, 24 pp.
  2. Ballard, H. E., J. de Paula-Souz and G. A. Wahlert. 2013. Violaceae. In The Families and Genera of Vascular Plants. Vol. XI. Kubitzki, K. (ed.), Springer, Berlin. Pp. 298-326.
  3. Choi, Y. K. and S. S. Whang. 2019. A comparative study of early leaf development in the Viola albida complex. Korean Journal of Plant Taxonomy 49: 1-7. https://doi.org/10.11110/kjpt.2019.49.1.1
  4. Doyle, J. J. and J. L. Doyle. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin 19: 11-15.
  5. Hama, E. 2002. Viola of Japan. Seibundo Shinkosha Publishing Co., Tokyo.
  6. Jang, S.-K. 2012. Phylogenetic study of Viola section Pinnatae Wang. Ph.D. dissertation, Kangwon National University, Chuncheon, Korea, 199 pp.
  7. Jang, S.-K., W.-T. Lee and K.-O. Yoo. 2006. Taxonomic study on Viola albida var. albida and its related taxa. Korean Journal of Plant Taxonomy 36: 163-187. https://doi.org/10.11110/kjpt.2006.36.3.163
  8. Kim, K. S. 1986. Studies of comparative morphology on the Korean Viola species. Ph.D. dissertation, Sungkyunkwan University, Suwon, Korea, 105 pp.
  9. Kim, K. S., B. Y. Sun, S. S. Whang and G. H. Chung. 1991. Biosystematic study on the genus Viola in Korea: Comparative morphology of the Viola albida complex. Korean Journal of Botany 34: 229-238.
  10. Kim, G.-T. and T.-W. Um. 1994. Effects of gibberelic acid treatment on seed germination of several Viola species. Applied Ecology Research 8: 74-77.
  11. Koo, J. C., H. J. Tak and S. S. Whang. 2010. Taxonomic study of Viola albida complex based on RAPD data. Korean Journal of Plant Taxonomy 40: 118-129. https://doi.org/10.11110/kjpt.2010.40.2.118
  12. Lee, C. B. 1999. Illustrated Flora of Korea. Hyangmunsa, Seoul, 791 pp.
  13. Lee, C. H. 2013. Exploitation of Korean native ornamental plants for new crops and investigation of their commercial feasibility: In vitro propagation and breeding of native flowers and ornamental trees. Report for 21C Frontier Research of Ministry of Science and ICT. Chungbuk National University, Cheongju, 323 pp.
  14. Lord, E. M. 1979. Physiological controls on the production of cleistogamous and chasmogamous flowers in Lamium amplexicaule L. Annals of Botany 44: 757-766. https://doi.org/10.1093/oxfordjournals.aob.a085790
  15. Park, C. W. 2009. The Genera of Vascular Plants of Korea. Academy Publishing Co., Seoul, 393 pp.
  16. Park, S.-C. 2012. Violets of Korea. Hamkkeganeun-gil, Seoul, 264 pp.
  17. Russell, N. H. 1960. Studies in the photoperiodic responses of violets (Viola). The Southwestern Naturalist 5: 177-186. https://doi.org/10.2307/3668940
  18. Srikanth, K. 2014. Differential expression of certain key genes mediates leaf morphology variation in Viola albida complex. Ph.D. dissertation, Jeonbuk National University, Jeonju, Korea, 153 pp.
  19. Srikanth, K., R. S. Hill and S. S. Whang. 2019. A correlation between leaf shape and its related key genes in Viola albida complex. In Vitro Cellular & Developmental Biology - Plant 55: 409-420. https://doi.org/10.1007/s11627-019-09975-5
  20. Whang, S. S. 2006. Analysis of ITS DNA sequences of the Viola albida complex. Korean Journal of Plant Resources 19: 628-633.
  21. White, T. J., T. Bruns, S. Lee and J. Taylor. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In PCR Protocols: A Guide to Methods and Applications. Innis, M. A., D. H.bGelfand, J. J. Sninsky and T. J. White (eds.), Academic Press, San Diego, CA. Pp. 315-322.
  22. Yoo K.-O. and S.-K. Jang. 2013. Korean Peninsula Violet Looking at Features. Gi-sung-sa Co.