• Journal of Microbiology and Biotechnology
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• v.9 no.3
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• pp.358-360
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• 1999

The effect of pectinase on menthol production by Mentha piperita in shake flasks was investigated. The optimum concentration of pectinase and period of elicitation for menthol production were 15 U/1 and 9 days, respectively. Pectinase elicitation at 15 U/1 for 9 days using a Lin-Staba medium with 2,4-dichlorophenoxyacetic acid (2,4-D) enhanced menthol production 37-fold (211.5 ㎎ menthol/l) with the specific menthol concentration (menthol concentration per unit weight of cells) of 27.5 ㎎/g dry cell weight (DCW). Our results also indicate that pectinase elicitation may activate the conversion of pulegone to menthol.

• Korean Journal of Medicinal Crop Science
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• v.25 no.2
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• pp.83-88
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• 2017

Background: This study was aimed to determine the optimal vine induction method for growing of Korean schisandra (Schisandra chinensis), by comparing plant growth and fruit yields between plants grown with either fence-type (U-type) or A-type induction. Methods and Results: Plants were transplanted on August 17, 2014, and the plant height, stem node number and weight were measured every two weeks, six times from June 17, 2016. The plant height, stem node number, and leaf length and width were higher with the A-type than with the U-type induction, by approximately 37.0%, 49.1%, 27.6%, and 12.7%, respectively. Although there was no significant difference between the photosynthesis rates of plants grown with the two vine induction method, the leaf area and leaf number per plant were higher in the plants grown with the A-type than the U-type, by approximately 23.7% and 46.0%, respectively. The number of green-color pixels, in a defined area of digital camera images of creeper leaves from the inducted vines, was significantly higher in the plants grown with the A-type than the U-type. The number of fruit clusters per plant was approximately 26 and 36, under the U-type and A-type, respectively. A two fold higher total fruit weight per plant was observed in the plants grown under the A-type (250 g/plant) than the U-type (120 g/plant). Conclusions: The A-type vine induction method is optimal for cultivation of Korean schisandra.

• Journal of Radiation Protection and Research
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• v.37 no.3
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• pp.116-122
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• 2012

A short-term atmospheric dispersion factor (${\chi}/Q$) is an essential element for radiological dose assessment following a hypothetical accidental releases of light-water nuclear power plants. The U. S. NRC developed PAVAN program to comply with the U. S. NRC's Regulatory Guide 1.145. Meteorological data is an essential element for atmospheric dispersion, and PAVAN uses a joint frequency distribution data, which represents the occurrence probability of wind speed and wind direction for atmospheric stability. Using the meteorological data measured at Kori and Wolsung sites for the last 5 years (from 2006 to 2010), a variety of joint frequency distribution data were prepared to evaluate ${\chi}/Q$ values with different wind speed classifications (U. S. NRC's recommendation and even distribution of occurrence probability) and periods of meteorological data to be analyzed (1 year, 2 year, 3 year, 4 year, 5 year). As a result, it was found that the influence of the wind speed classification on ${\chi}/Q$ values is little, while the influence of the periods of meteorological data to be analyzed is relatively significant, representing more than 1.5 times in the ratio of maximum to minimum values.

• Proceedings of the Korean Society of Crop Science Conference
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• 1989.05a
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• pp.36-37
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• 1989

• Neonatal Medicine
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• v.16 no.1
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• pp.47-54
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• 2009

Purpose: Present evidences suggest that Ureaplasma urealyticum is a cause of pneumonia, septicemia, and bronchopulmonary dysplasia (BPD) in newborn infants, particularly those born prematurely. The purpose of this work was to examine the relationship between Ureaplasma urealyticum in the tracheal aspirates and adverse outcomes, such as BPD and early onset neonatal sepsis in premature infants. Methods: A polymerase chain reaction (PCR) was performed on tracheal aspirates collected within 24 hour after birth in 176 premature infants less than 35 weeks of gestation and admitted to the neonatal intensive care unit of Bundang CHA Hospital. Results: U. urealyticum was detected in 37 of 176 preterm infants (21.0%). Gestational age ($29^{+5}{\pm}2^{+5}$ wk vs. $30^{+6}{\pm}2^+{-5}$ wk, P=0.0l3) and birth weight (1.39${\pm}$0.44 kg vs. 1.59${\pm}$0.55 kg, P=0.037) were lower in the U urealyticum-positive group compared to the control group. The incidence of early onset neonatal sepsis (16.2% vs. 6.5%, P=0.045) and BPD (45.9% vs. 29.5%, P=0.047) was higher in the U urealyticum-positive group compared to the control group, but the severity of BPD was not different between two groups. However, multiple logistic regression analysis revealed that the presence of U. urealyticum was not independently related to the development of early onset neonatal sepsis and BPD. Conclusion: The results suggest that colonization of the lower respiratory tract by U. urealyticum might not be related to the development of neonatal sepsis and BPD directly in preterm infants.

• Journal of Korean Neurosurgical Society
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• v.37 no.1
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• pp.48-53
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• 2005

Objective: The purpose of this study is to elucidate in vitro responses to combined gamma knife irradiation and p53 gene transfection on human malignant glioma cell lines. Methods: Two malignant human glioma cell lines, U87MG (p53-wild type) and U373MG (p53-mutant) were transfected with an adenoviral vector containing p53 (MOI of 50) before and after applying 20Gy of gamma irradiation. Various assessments were performed, including, cell viability by MTT assay; apoptosis by annexin assay; and cell cycle by flow cytometry, for the seven groups: mock, p53 only, gamma knife (GK) only, GK after LacZ, LacZ after GK, GK after p53, p53 after GK. Results: Cell survival decreased especially, in the subgroup transfected with p53 after gamma irradiation. Apoptosis tended to increase in p53 transfected U373 MG after gamma irradiation (apoptotic rate, 38.9%). The G2-M phase cell cycle arrest markedly increased by transfecting with p53, 48 hours after gamma knife irradiation in U373 MG (G2-M phase, 90.8%). Conclusion: These results suggest that the in vitro effects of combined gamma knife irradiation and p53 gene transfection is an augmentation of apoptosis and G2-M phase cell cycle arrest, which are more exaggerated in U373 MG with p53 transfection after gamma knife irradiation.

• Journal of Radiopharmaceuticals and Molecular Probes
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• v.5 no.2
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• pp.101-112
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• 2019

Herein, 2-nitroimidazole-fluorophore conjugates were synthesized by linking 2-nitroimidazole and FITC or RITC via thiourea bonds. The prepared derivatives were stable for 2 h in Dulbecco's modified Eagle's medium (DMEM) at 37 ℃. The novel conjugates were studied for their in vitro uptake under hypoxic conditions using U87MG and CT-26 cell lines, showing significantly higher uptakes in hypoxic than normoxic cells. Immunohistochemical analysis confirmed hypoxia in U87MG and CT-26 xenografted tumor tissues. Moreover, the prepared conjugates were evaluated by in vivo experiments after intravenous injection in U87MG and CT-26 xenografted mice. Hypoxia was confirmed by immunohistochemistry of the prepared derivatives with co-injected pimonidazole. Confocal microscopy of the prepared derivatives showed strong fluorescence in hypoxic tumor tissues correlated with the pimonidazole distribution. This suggested that the 2-nitroimidazole-fluorophore conjugates are promising optical imaging probes for tumor hypoxia and are promising substitutes for pimonidazole immunohistochemistry, which requires a multi-step procedure of incubation involving antibody, second antibody, dye, hydrogen peroxide, and multiple washing steps.

• Nuclear Engineering and Technology
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• v.51 no.8
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• pp.1951-1956
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• 2019

As a part of the preliminary decommissioning plan of KEPCO-NF fuel fabrication facility, DCGLs of three target radionuclides, ²³⁴U, ²³⁵U, and ²³⁸U, were derived using RESRAD-BUILD code and contaminated areas of the facility were classified based on contamination levels from the derived DCGLs. From code simulations, one-room modeling results showed that the grinding room in building #2 was the most restrictive (DCGL_gross = 10493.01 Bq/㎡). The DCGL_gross results in contaminated areas from one-room modeling were slightly more conservative than three-room modeling. Prior to the code simulation, field survey and measurements conducted by each survey unit. For a conservative approach, the most restrictive DCGL_gross in each survey unit was taken as a reference to classify the contaminated areas of the facility. Accordingly, seven rooms and 37 rooms in the nuclear-fuel buildings were classified as Class 1 and Class 2, respectively. As expected, fuel material handling and processing rooms such as the grinding room, sintering room, compressing room, and powder collecting room were included in the Class 1 area.

• ETRI Journal
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• v.37 no.5
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• pp.945-950
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• 2015

A novel microstrip low-pass filter is presented to achieve an ultra-wide stopband with 11 harmonic suppression and very sharp skirt characteristics. The filter is composed of a modified U-shaped resonator (which creates two fully adjustable transmission zeroes), a T-shaped resonator (which determines a cut-off frequency), and four radial stubs (which provide a wider stopband). The operating mechanism of the filter is investigated based on a proposed equivalent-circuit model, and the role of each section of the proposed filter in creating null points is theoretically discussed in detail. The presented filter with 3 dB cut-off frequency ($f_c=2.35GHz$) has been fabricated and measured. Results show that a relative stopband bandwidth of 164% (referred to as a 22 dB suppression) is obtained while achieving a high figure-of-merit of 15,221.

• Journal of agriculture & life science
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• v.46 no.1
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• pp.163-176
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• 2012

TTo increase productivity of a strong extracellular alkaline protease (BCAP), stable strains of Bacillus clausii I-52 carrying another copy of BCAP gene in the chromosome were developed. Integrative vector, pHPS9-fuBCAP carrying BCAP promoter, ribosome binding site, signal sequence and active protease gene was constructed and transferred into B. clausii I-52, and integration of the constructed plasmid into chromosome was identified by PCR. An investigation was carried out on BCAP production by B. clausii I-52 and transformant C5 showing the highest relative activity of alkaline protease using submerged fermentation. Maximum enzyme activity was produced when cells were grown under the submerged fermentation conditions at $37^{\circ}C$ for 48 h with an aeration rate of 1 vvm and agitation rate of 650 rpm in a optimized medium (soybean meal 2%, wheat flour 1%, sodium citrate 0.5%, $K_2HPO_4$ 0.4%, $Na_2HPO_4$ 0.1%, NaCl 0.4%, $MgSO_47H_2O$ 0.01%, $FeSO_47H_2O$ 0.05%, liquid maltose 2.5%, $Na_2CO_3$ 0.6%). A protease yield of approximately 134,670U/ml was achieved using an optimized media, which show an increase of approximately 1.6-fold compared to that of non-transformant (83,960 U/ml). When the stability of transformant C5 was examined, the integrated plasmid pHPS9-fuBCAP was detected in the transformant after cultivation for 8 days, suggesting that it maintained stably in the chromosomal DNA of transformant C5.