Journal of Korean Neurosurgical Society

The Korean Neurosurgical Society (대한신경외과학회)
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The Combined Effect of Gamma Knife Irradiation and p53 Gene Transfection in Human Malignant Glioma Cell Lines

  • Kim, Jeong-Eun (Departments of Neurosurgery, Seoul National University College of Medicine) ;
  • Paek, Sun-Ha (Departments of Neurosurgery, Seoul National University College of Medicine) ;
  • Kim, Dong-Gyu (Departments of Neurosurgery, Seoul National University College of Medicine) ;
  • Chung, Hyun-Tai (Departments of Neurosurgery, Seoul National University College of Medicine) ;
  • Kim, Young-Yim (Departments of Clinical Research Institute, Seoul National University College of Medicine) ;
  • Jung, Hee-Won (Departments of Neurosurgery, Seoul National University College of Medicine)
  • Published : 2005.01.31
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Abstract

Objective: The purpose of this study is to elucidate in vitro responses to combined gamma knife irradiation and p53 gene transfection on human malignant glioma cell lines. Methods: Two malignant human glioma cell lines, U87MG (p53-wild type) and U373MG (p53-mutant) were transfected with an adenoviral vector containing p53 (MOI of 50) before and after applying 20Gy of gamma irradiation. Various assessments were performed, including, cell viability by MTT assay; apoptosis by annexin assay; and cell cycle by flow cytometry, for the seven groups: mock, p53 only, gamma knife (GK) only, GK after LacZ, LacZ after GK, GK after p53, p53 after GK. Results: Cell survival decreased especially, in the subgroup transfected with p53 after gamma irradiation. Apoptosis tended to increase in p53 transfected U373 MG after gamma irradiation (apoptotic rate, 38.9%). The G2-M phase cell cycle arrest markedly increased by transfecting with p53, 48 hours after gamma knife irradiation in U373 MG (G2-M phase, 90.8%). Conclusion: These results suggest that the in vitro effects of combined gamma knife irradiation and p53 gene transfection is an augmentation of apoptosis and G2-M phase cell cycle arrest, which are more exaggerated in U373 MG with p53 transfection after gamma knife irradiation.

Keywords

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