• Proceedings of the Korean Society of Applied Pharmacology
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• 2001.11a
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• pp.108-108
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• 2001

Axin2/Conductin/Axil and its ortholog Axin are negative regulators of the Wnt signaling pathway, which promote the phosphorylation and degradation of ${\beta}$-catenin. While Axin is expressed ubiquitously, Axin2 mRNA was seen in a restricted pattern during mouse embryogenesis and organogenesis. Because many sites of Axin2 expression overlapped with those of several Wnt genes, we tested whether Axin2 was induced by Wnt signaling. Endogenous Axin2 mRNA and protein expression could be rapidly induced by activation of the Wnt pathway, and Axin2 reporter constructs, containing a 5.6 kb DNA fragment including the promoter and first intron, were also induced. This genomic region contains eight Tcf/LEF consensus binding sites, five of which are located within longer, highly conserved non-coding sequences. The mutation or deletion of these Tcf/LEF sites greatly diminished induction by ${\beta}$-catenin, and mutation of the Tcf/LEF site T2 abolished protein binding in an electrophoretic mobility-shift assay. These results strongly suggest that Axin2 is a direct target of the Wnt pathway, mediated through Tcf/LEF factors. The 5.6 kb genomic sequence was sufficient to direct the tissue specific expression of d2EGFP in transgenic embryos, consistent with a role for the Tcf/LEF sites and surrounding conserved sequences in the in vivo expression pattern of Axin2. Our results suggest that Axin2 participates in a negative feedback loop, which could serve to limit the duration or intensity of a Wnt-initiated signal.

• Journal of Industrial Technology
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• v.3
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• pp.53-63
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• 1983

The probability of failure is used to analyze the reliability of three dimensional slope failure, instead of conventional factor of safety. The strength parameters are assumed to be normal variated and beta variated. These are interval estimated under the specified confidence level and maximum likelihood estimation. The pseudonormal and beta random variables are generated using the uniform probability transformation method according to central limit theorem and rejection method. By means of a Monte-Carlo Simulation, the probability of failure is defined as; Pf=M/N N : Total number of trials M : Total number of failures Some of the conclusions derived from the case study include; 1. Three dimensional factors of safety are generally much higher than 2-D factors of safety. However situations appear to exist where the 3-D factor of safety can be lower than the 2-D factor of safety. 2. The F3/F2 ratio appears to be quite sensitive to c and ${\phi}$ and to the shape of the 3-D shear surface and the slope but not to be to the unit weight of soil. 3. In cases that strength parameters are assumed to be normal variated and beta variated, the relationships between safety factor and the probability of failure are fairly consistent, regardless of the shape of the 3-D shear surface and the slope. 4. As the c-value is increased, the probability of failure for the same safety factor is increased and as the ${\phi}-value$ is increased, the probability of failure for the same safety factor is decreased.

• Biotechnology and Bioprocess Engineering:BBE
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• v.11 no.2
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• pp.146-153
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• 2006

A passive microfluidic delivery system using hydrophobic valving and pneumatic control was devised for microfluidic handling on a chip. The microfluidic metering, cutting, transport, and merging of two liquids on the chip were correctly performed. The error range of the accuracy of microfluid metering was below 4% on a 20 nL scale, which showed that microfluid was easily manipulated with the desired volume on a chip. For a study of the feasibility of biochemical reactions on the chip, a single enzymatic reaction, such as ${\beta}-galactosidase$ reaction, was performed. The detection limit of the substrate, i.e. fluorescein $di-{\beta}-galactopyranoside$ (FDG) of the ${\beta}-galactosidase$ (6.7 fM), was about 76 pM. Additionally, multiple biochemical reactions such as in vitro protein synthesis of enhanced green fluorescence protein (EGFP) were successfully demonstrated at the nanoliter scale, which suggests that our microfluidic chip can be applied not only to miniaturization of various biochemical reactions, but also to development of the microfluidic biochemical reaction system requiring a precise nano-scale control.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.3 no.3
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• pp.71-86
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• 1983

Current R.C. retaining wall design is bared on WSD, but the reliability based design method is more rational than the WSD. For this reason, this study proposes a reliability based design criteria for the cantilever retaining wall, which is most common type of retaining wall, and also proposes the theoretical bases of nominal safety factors of stability analysis by introducing the reliability theory. The limit state equations of stability analysis and design of each part of cantilever retaining wall are derived and the uncertainty measuring algorithms of each equation are also derived by MFOSM using Coulomb's coefficient of the active earth pressure and Hansen's bearing capacity formula. The levels of uncertainties corresponding to these algorithms are proposed appropriate values considering our actuality. The target reliability indices (overturning: ${\beta}_0$=4.0, sliding: ${\beta}_0$=3.5, bearing capacity: [${\beta}_0$=3.0, design for flexure: [${\beta}_0$=3.0, design for shear: ${\beta}_0$=3.2) are selected as optimal values considering our practice based on the calibration with the current R.C. retaining wall design safety provisions. Load and resistance factors are measured by using the proposed uncertainties and the selected target reliability indices. Furthermore, a set of nominal safety factors, allowable stresses, and allowable shear stresses are proposed for the current WSD design provisions. It may be asserted that the proposed LRFD reliability based design criteria for the R.C. retaining wall may have to be incorporated into the current R.C. design codes as a design provision corresponding to the USD provisions of the current R.C. design code.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.4 no.3
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• pp.75-83
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• 1984

The probability of failure is used to analyze the reliability of three dimensional slope failure, instead of conventional factor of safety. The strength parameters are assumed to be normal variated and beta variated. These are interval estimated under the specified confidence level and maximum likelihood estimation. The pseudonormal and beta random variables are generated using the uniform probability transformation method according to central limit theorem and rejection method. By means of a Monte-Carlo Simulation, the probability of failure is defined as; $P_f=M/N$ N: Total number of trials M: Total number of failures Some of the conclusions derived. from the case study include; 1. Three dimensional factors of safety are generally much higher than 2-D factors of safety. However situations appear to exist where the 3-D factor of safety can be lower than the 2-D factor of safety. 2. The $F_3/F_2$ ratio appears to be quite sensitive to c and ${\phi}$ and to the shape of the 3-D shear surface and the slope but not to be to the unit weight of soil. 3. From the two models (normal, beta) considered for the distribution of the factor of safety, the beta distribution generally provides lager than normal distribution. 4. Results obtained using the beta and normal models are presented in a nomgraph relating slope height and slop angle to probability of failure.

• Journal of Food Hygiene and Safety
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• v.20 no.3
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• pp.141-146
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• 2005

A simple and sensitive analysis method based on reverse phase (RP) HPLC with UV detector was developed for simultaneous determination of chlorophyll a and b, pheophorbide a and $\beta-Carotene$ in Chlorella and Spirulina products. For added concentration $(50\;\mug/ml)$ of chlorophyll a and b, pheophorbide a and $\beta-Carotene$, recoveries of those were 70.3, 71.6, 60.1 and $90.5\%$, respectively, with relative standard deviations of 2.8,6.0, 10.6 and $10.4\%$. Limit of detection and quantification had ranges of $0.1\sim1.0\;\mug/ml$ and $0.2\sim2.0\;\mug/ml$, respectively. Calibration curve was linear with correlation coefficient of 0.995 for chlorophyll a and b, pheophorbide a and $\beta-Carotene$. Results of simultaneous determination in Chlorella and Spirulina products were showed ranges of $121.g\sim543.0\;\mug/ml$ for chlorophyll a,$0.6\sim160.0\;\mug/ml$ for chlorophyll b, $19.2\sim60.3\;\mug/ml$ for pheophorbide a and $383.6\sim1713.7\;\mug/ml$ for $\beta-Carotene$, respectively. Chlorophyll b contents in Chlorella products were detected above 30 times level to those in Spirulina products. $\beta-Carotene$ contents in Spirulina products were detected 2.7 times level to those in Chlorella products.

• Journal of Periodontal and Implant Science
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• v.29 no.4
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• pp.979-995
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• 1999

This study was investigated to observe the effect of Treponema denticola cell sonicates(TDC) and Treponema lecithinolyticum cell sonicates(TLC) on cytokine secretion and matix metalloproteinase-2(MMP-2) activation of cultured human gingival fibroblast. Several experiments were performed including $IL-1{\beta}$, IL-6 ELISA for the effect on the $IL-1{\beta}$, IL-6 secretion of human gingival fibroblast. Also gelatinase zymography and gelatin dissolubility test for the activation of MMP-2 secreted by gingival fibroblast. The results were as follows. 1. The effect of TDC and TLC on IL-6 secretion of human gingival fibroblast showed statistically significant increase of IL-6 secretion in the TDC and TLC treated group compared to no treatment group(p<0.05) . 2. The amount of $IL-1{\beta}$ secretion was below the lower limit and there was no difference in the $IL-1{\beta}$ secretion of gingival fibroblast between TDC, TLC treated group and no treatment group. 3. The active form of pro MMP-2 with 72 kDa molecular weight was activated in both TDC and TLC treated group and clear band was appeared at 62kDa site on the zymography. 4. Gelatin dissolubility of MMP-2 secreted by gingival fibroblast was higher in TDC and TLC treated group compared to no treatment group(p<0.05). 5. In the TDC treated group, serine protease of T. denticola affect gelatin dissolubility. But in the TLC treated group gelatin was degraded by only MMP secreted by gingival fibroblast. Regarding to the above results, TDC and TLC have an effect on the IL-6 secretion increase of human gingival fibroblast and appears to activate pro MMP-2 which degrades collagen.

• Journal of the Korea Concrete Institute
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• v.11 no.5
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• pp.131-137
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• 1999

The use of higher strength materials with the strength methed of design has resulted in more slender member and shallower sections. For this reason, it is necessary to satisfy the requirements of serviceability even though the structural safety is the most important limit state. This paper is only concerned with the control of deflections in the serviceability. In this study, an analytical model is presented to predict the deflections of reinforced concrete beams to given loading and environmental conditions. This model is based on the finite element approach in which a finite element is generally divided into a number of stiffening effect due to cracking, creep and shrinkage. Comparisons are made with available measured deflections reported by others to assess the capability of the layered beam model. The calculated values of instantaneous and long-term deflection show good agreement with experimental results in the range of tension stiffening parameter $\beta$ between 2.5 and 3.0.

• Transactions of the Korean Society of Pressure Vessels and Piping
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• v.5 no.1
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• pp.14-19
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• 2009

NDE(Nondestructive examination) detects a flaw or discontinuity in materials. Flaws detected by the examination shall be evaluated for the decision basis of the integrity. The internal flaws of forging products can be detected by UT. However, UT has detection limits because of its reflected signal weakness. Normally, a 1mm or less flaw is known as the limit. If a flaw was detected, the size of flaw would be evaluated by AVG(or DGS) technique. To verify the evaluation data, alternative examination methods are needed. But there is no alternative examination methods until now. In this study, Phased array ultrasonic technique can be used to size the flaws in the generator rotor with focused beam of ultrasonic wave as a supplement method of AVG. Also, the phased array ultrasonic technique described enables the shape of flaw to be depicted exactly.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2002.11a
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• pp.331.1-331
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• 2002

Four degrees of freedom mathematical model is presented to describe the fundamental mechanisms of the disc brake squeal noise. A contact parameter is introduced to describe the coupling between the in-plane and the out-of-plane motions. The friction coeficient including "relative velocity" and ′normal force" can be generally formulated as the form of multiplication with polynominal parameters(${\beta}$, ${\gamma}$). (omitted)