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Studies on Simultaneous Determination of Chlorophyll a and b, Pheophorbide a, and $\beta-Carotene$ in Chlorella and Spirulina Products  

Lee Young Ja (Seoul Regional Food and Drug Administration)
Kim So Hee (Seoul Regional Food and Drug Administration)
Kim Jin-Sook (Seoul Regional Food and Drug Administration)
Han Jeong A (Seoul Regional Food and Drug Administration)
Seo Hae Jeom (Seoul Regional Food and Drug Administration)
Lim Hyo Jeong (Seoul Regional Food and Drug Administration)
Choi Soo Young (Seoul Regional Food and Drug Administration)
Publication Information
Journal of Food Hygiene and Safety / v.20, no.3, 2005 , pp. 141-146 More about this Journal
Abstract
A simple and sensitive analysis method based on reverse phase (RP) HPLC with UV detector was developed for simultaneous determination of chlorophyll a and b, pheophorbide a and $\beta-Carotene$ in Chlorella and Spirulina products. For added concentration $(50\;\mug/ml)$ of chlorophyll a and b, pheophorbide a and $\beta-Carotene$, recoveries of those were 70.3, 71.6, 60.1 and $90.5\%$, respectively, with relative standard deviations of 2.8,6.0, 10.6 and $10.4\%$. Limit of detection and quantification had ranges of $0.1\sim1.0\;\mug/ml$ and $0.2\sim2.0\;\mug/ml$, respectively. Calibration curve was linear with correlation coefficient of 0.995 for chlorophyll a and b, pheophorbide a and $\beta-Carotene$. Results of simultaneous determination in Chlorella and Spirulina products were showed ranges of $121.g\sim543.0\;\mug/ml$ for chlorophyll a,$0.6\sim160.0\;\mug/ml$ for chlorophyll b, $19.2\sim60.3\;\mug/ml$ for pheophorbide a and $383.6\sim1713.7\;\mug/ml$ for $\beta-Carotene$, respectively. Chlorophyll b contents in Chlorella products were detected above 30 times level to those in Spirulina products. $\beta-Carotene$ contents in Spirulina products were detected 2.7 times level to those in Chlorella products.
Keywords
chlorophyll a and b; pheophorbide a; B-carotene; Chlorella; Spirulina;
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