Because of financial and safety concerns, there are needs for more accurate prediction of bridge behavior. Underestimation of the bridge load carrying capacity can have serious economic consequences, as deficient bridges must be repaired or rehabilitated. Therefore, the knowledge of the actual bridge behavior under live load may lead to a more realistic calculation of the load carrying capacity and eventually this may allow for more bridges to remain in service with or without minor repairs. The presented research is focused on the reliability evaluation of the actual load carrying capacity of existing bridges based on the field testing. Seventeen existing bridges were tested under truck load to confirm their adequacy of reliability. The actual response of existing bridge structures under live load is measured. Reliability analysis is performed on the selected representative bridges designed in accordance with AASHTO codes for bridge component (girder). Bridges are first evaluated based on the code specified values and design resistance. However, after the field testing program, it is possible to apply the experimental results into the bridge reliability evaluation procedures. Therefore, the actual response of bridge structures, including unintentional composite action, partial fixity of supports, and contribution of nonstructural members are considered in the bridge reliability evaluation. The girder distribution factors obtained from the tests are also applied in the reliability calculation. The results indicate that the reliability indices of selected bridges can be significantly increased by reducing uncertainties without sacrificing the safety of structures, by including the result of field measurement data into calculation.