Objectives: The purpose of this study is to gain the objective indicators for the classification of hypertension by oriental medical pulse diagnosis, through finding out the parameters which can distinguish the pulse of hypertensive patient from that of normal subject, and characterizes the wiry pulse of hypertension. Methods: 30-59 yr, male, 946 healthy volunteers and 35 hypertensive patients were enrolled in this study. All the hypertensive patients were taking medicine to control the blood pressure and the blood pressure of the hypertension group was not statistically different from that of the normal healthy group. Data were acquired using 3 dimensional pulse imaging analyser(DMP-3000, DAEYOMEDI, KOREA) and analysed according to the age bands and the applied pressure levels. Results: 1. RAI/t, w/t and t2/t decreased with the levels of applied pressure and increased with age in the normal healthy group, not in the hypertension group. 2. RAI/t, w/t, t2/t were significantly higher in the hypertension group than the normal healthy group in the 30-age band, and elasticity coefficient was higher in the hypertension group than the normal healthy group in the 40-age band. 3. Researches on the patients without hypotensive agents are needed to figure out whether these parameters are the components of hypertensive wiry pulse. Conclusions: Analysing the radial pulse at 5 applied pressure levels using 3 dimensional pulse imaging analyser may be useful to differentiate the pulses of the hypertensive patients from those of the normal subjects, and characterize the hypertension.

Objectives: The information on the depth where pulse wave appears is as important as pulse waveform. The aim of this study was to classify pulse pattern using pressure-height(P-H) volume-curve by 5 applied pressure levels to find out the information on the depth of pulse and interpret the floating & sinking pulse in oriental medical pulse diagnosis. Methods: We used 3 dimensional pulse imaging analyser (DMP-3000, DAEYOMEDI Co., Korea), which measures radial pulse waveforms noninvasively by way of tonometric method at 5 applied pressure levels, and shows P-H volume-curves by applied pressure. 448 subjects were enrolled, pulse waveforms were measured and the P-H volume-curves were gained on the three locations of Chon, Kwan, and Cheok. Results: Gained P-H volume curves were classified into 3 types ; increase type, decrease type, and increase-decrease type. Increase-decrease type appeared more often on Chon and Kwan, while increase type appeared more often on Cheok. In a few cases, decrease-type appeared on Chon and Kawn, however it never appeared on Cheok. Conclusions: Through the classification of pulse by P-H volume-curve, we gained the information on the depth of pulse. We speculate the decrease type as floating pulse, the increase-decrease type as middle pulse, and the increase type as sinking pulse in oriental medical pulse diagnosis. After more researches on P-H volume-curve by applied pressure, the P-H volume-curve may be used as an important factor for pulse diagnosis.

목적 : 본 연구는 간접구의 안전성에 대한 기초 데이터를 확보하기 위하여 수행되었다. 연구설계 : 50명의 건강한 성인을 무작위 배정으로 두 그룹으로 나누어 4주간 (일주일에 3회) 신궐혈과 관원혈에 뜸을 시술하였다. 시험군에 비하여 대조군은 열이 전달되지 않도록 단열제로 처리한 뜸으로 동일한 부위에 시술하였다. 첫 시술 1시간 전과 마지막 시술 1시간 후에 공복상태에서 채혈을 하여 혈액학적 검사를 시행하였다. 결과 : 혈액학적 검사에서 적혈구와 총 백혈구 및 백혈구의 종류별 절대수치와 상대수치에서 두 그룹 간에 특별한 차이가 없었다. 또한 혈청학적 검사에서 총 단백질, 알부민, AST, ALT, ALP, GGT, LDH, 요소질소, 크레아티닌, 총콜레스테롤 및 중성지방의 비교에서도 그룹 간에 차이가 없었다. 결론 : 본 연구는 건강한 사람에서 간접구의 시행 후 혈액학적 안전성을 처음으로 보고하는 것으로서, 향후 뜸의 연구를 수행시 중요한 비교 자료로 사용될 수 있을 것이다.

Objectives: To investigate the anti-inflammatory effects of cultivated wild ginseng pharmacopuncture in lipopolysaccharide (LPS)-induced inflammatory rat model. Methods: Sprague-Dawley rats were divided into 4 groups; LPS control (n=6), LPS+cultivated wild ginseng pharmacopuncture at CV4 (n=6), LPS+cultivated wild ginseng pharmacopuncture at CV17 (n=6), and LPS+cultivated wild ginseng pharmacopuncture at Ex-HN1 (n=6). Pharmacopuncture (0.1 ml) was given every two days for 4 weeks followed by inflammation induction by peritoneal LPS injection (5 mg/kg). Blood, liver tissue, and peritoneal lavage fluid were taken and proinflammatory cytokines and other related factors were analysed. Results: Compared with the control group, CV4 and Ex-HN1 pharmacopuncture groups significantly attenuated plasma IL-$1{\beta}$, IL-6, and TNF-$\alpha$ increase at 2h and 5h after LPS injection (P<0.05). A significant difference from control group emerged at 5 h for plasma IL10 (P<0.05). For liver cytokines analyzed at 5 h after LPS injection, only CV4 pharmacopuncture group showed significant difference in TNF-$\alpha$ and IL-10 (P<0.05). Blood CD4/CD8 ratio and the phagocytic activities of polymorphonuclear neutrophils were not different from those of control group in all pharmacopuncture groups (P>0.05). CV4 pharmacopuncture significantly attenuated increase of plasma ${NO_3}^-/{NO_2}^-$, Intracellular adhesion molecule-1 (ICAM-1), cytokine-induced neutrophil chemoattractant-1 (CINC-1), and prostaglandin $E_2$ ($PGE_2$) compared with the control group (P<0.05). Monocyte chemoattractant protein-1, $PGE_2$, and CINC-1 level of CV4 pharmacopuncture group was significantly different from those from the control group (P<0.05). Conclusions: These results indicate that cultivated wild ginseng pharmacopuncture at CV4 may have a potent anti-inflammatory effect in an LPS-induced inflammatory rat model.

Objective: This study was performed to investigate the effect of manual acupuncture, invasive laser acupuncture and laser skin irradiation at LU8, LR4, HT8 and LR2(Liver Seunggyeok) on D-galN-induced liver injury in rats. Method: Liver injury was induced with D-galN. The experimental rats were divided four groups(Control group, EXP-1, EXP-2, EXP-3). In the Control group, liver injury-induced and not treated. EXP-1 group was liver injury-induced and carried out manual acupuncture with Young-Su(against the meridian course and following the course of the meridian) & Won-Bang(by twisting and rotating the needle) acupuncture method at Liver Seunggyeok. EXP-2 group was liver injury-induced and carried out invasive laser acupuncture at Liver Seunggyeok. EXP-3 group was liver injury-induced and carried out laser skin irradiation at Liver Seunggyeok. Result: In the change of body weight(in 1 week), EXP-1, EXP-2 and EXP-3 groups were significantly increased as compared with control group. In the change AST & ALT, EXP-1 and EXP-2 groups were significantly decreased as compared with control group. In the change of SOD, EXP-1, EXP-2 and EXP-3 groups were significantly increased as compared with control group. In the change of WBC, EXP-2 group was significantly increased as compared with control group. Conclusion: Manual acupuncture, invasive laser acupuncture and laser skin irradiation at Liver Seunggyeok had hepatotherapeutic effect on the treatment of hepatocytotoxity. Invasive laser acupuncture was as effective as manual acupuncture on the treatment of hepatocytotocity.

Objective & Method: The purpose of this study is to investigate the effects of herbal-acupuncture with Corni Fructus extract (CF-HA) at Eumgok(KI10) on osteoporosis in ovariectomized (OVX) ddy mice. I carried out several experimental items to analyze the changes in body weight, uterine weight, uterus index, tibial length, the ash bone weight, tibial BMD, the levels of serum ALP, osteocalcin, Ca, P and the levels of tibial Ca, P, Ca/P ratio, and we performed histological and histomorphological analysis as well. Result: 1. CF-HA at Eumgok(KI10) significantly did not increase the level of bone mineral density in overiectomized mice. 2. CF-HA at Eumgok(KI10) significantly decreased the level of serum phosphorus in ovariectomized mice and sinificantly increased the level of serum calcium in ovariectomized mice. 3. CF-HA at Eumgok(KI10) significantly increased the levels of tibial calcium and phosphorus in ovariectomized mice. 4. CF-HA at Eumgok(KI10) significantly decreased the number of tibial osteoclast like cell in ovariectomized mice. 5. In the histomorphometric analysis of tibia, GPL(growth plate length) was significantly decreased by CF-HA at Eumgok(KI10) in ovariectomized mice. Conclusions: These results suggest that CF-HA at Eumgok(KI10) may have useful therapy effects on osteoporosis in ovariectomized mice.

Objective & Methods: The purpose of this study is to investigate the anti-oxidative and immuneregulative effects of electro-acupouncture(EA) at SP6(Sameumgyo) in aged rats. The author performed several experimental items including blood cell counts, blood chemistry, measurement of various oxidants and antioxidants in liver and spleen, analysis of various cytokines in spleen. The results are as follows. Results: 1. EA at SP6 significantly reduced the number of platelets in blood. 2. EA at SP6 significantly reduced NO concentration and significantly increased catalase activity in liver. 3. EA at SP6 significantly reduced NO concentration and significantly increased SOD activity, catalase activity and glutathione concentration in spleen. 4. EA at SP6 restored the increase of IL-4, IL-6 and the decrease of IFN-$\gamma$ in aged rat spleen. Conclusion: According to these results, it is postulated that EA at SP6 has an antioxidative effect through increasing the activities of antioxidative enzymes and inhibiting production of oxidized substances, as well as an immune regulative effect in aging process. In consequence, it is presumed that EA at SP6 may have an anti-aging effect.

Objective & Methods: The purpose of this study is to investigate the effects of Artemisiae Capillaris Herba herbal-acupuncture (ACH-HA) at GB34 (Yangleungchean) on hyperlipidemia induced with alloxan injection and high fat diet in rats. The author performed several experimental items to analyze the levels of various components and enzymes in serum, liver, as well as the histological changes of liver and aorta. Results: 1. ACH-HA solution increased the DPPH radical scavenging activity in rat liver cells. 2. ACH-HA at GB34 significantly decreased the levels of serum total cholesterol, low-density lipoprotein (LDL)-cholesterol, free cholesterol and atherogenic index (AI), while significantly increased the ratios of high-density lipoprotein (HDL)/total cholesterol and phospholipid/total cholesterol in hyperlipidemic rats. 3. ACH-HA at GB34 significantly decreased serum aspartate aminotransferase (AST) level in hyperlipidemic rats. 4. ACH-HA at GB34 significantly increased hepatic glutathione (GSH) activity in hyperlipidemic rats. Conclusion: From the above results, it is suggested that ACH-HA at GB34 may have therapeutic and preventive effects on hyperlipidemia.

Objectives: The purpose of this study was to identify the effectiveness of neuronal activities for the acupuncture and laser acupuncture application. Methods: The subject were divided into 7 groups as control group without acupuncture, acupuncture treatment with tonify manipulation with the direction of channel at HT9, LR1(AT-A), acupuncture treatment with purge manipulation against the direction of channel at HT3, Kl10(AT-B), acupuncture treatment with tonify manipulation with the direction of channel at HT9, LR1 and purge manipulation against the direction of channel at HT3, KI10(AT-C), laser acupuncture treatment with red light 658 nm at HT9, LR1(LAT-A), laser acupuncture treatment with green light 532 nm at HT3, KI10(LAT-B), laser acupuncture treatment with red light 658 nm at HT9, LR1 and green light 532 nm at HT3, KI10(LAT-B). Antiapopotic effect of acupuncture was observed by Bax, Bcl-2 and cytochrome C. Neuroprotective effect of acupuncture was observed by cresyl violet and ChAT. Results: AT-A, AT-B, AT-C, LAT-A, LAT-B and LAT-C groups were significantly increased comparing the control groups in expression ChAT and in neuroprotective effect by cresyl violet. AT-A, AT-B, AT-C, LAT-A, LAT-B and LAT-C groups were significantly decreased comparing the control groups in expression Bax. AT-C, LAT-A, LAT-B and LAT-C groups were significantly increased comparing the control groups in expression Bcl-2. AT-A, AT-B, AT-C, LAT-A, LAT-B and LAT-C groups were significantly decreased comparing the control groups in Bax/Bcl-2 ratio. LAT-B and LAT-C groups were significantly decreased comparing the control groups in expression cytochrome C. Conclusions: The acupuncture with tonify and purge manipulation and laser acupuncture with red and green light could be effective for antiapopotic and neuroprotective effect in focal brain ischemia.

Objectives: This research aims to know the current status and prospect of traditional medical market to provide sources in setting a development strategy and making a policy on the traditional medicine Methods: This research categorized 11,611 clinics registered in telephone directory as of 2008 by region and some clinics were extracted with simple random method. The inquiry was made with questionnaires and analyzed on the base of frequency. Results: 1. The number of the employee in a clinic were slightly decreased from 2.9 in 2005 and 2006 to 2.8 in 2007. That of the patients also showed a downward tendency from 11,811 in 2005 through 11,716 in 2006 to 11,344 in 2007. 2. An annual turnover in a clinic amounted to 257 million won in 2007 from 232 million won in 2005 through 245 million won in 2006. 3. Approximately 92% of the responses made a negative diagnosis on the traditional medical market and 21% of responses indicated the overall economic recession as a primary reason in their opinions. 4. About 29% of responses presented the extension of medical insurance on traditional medicine as a prior policy. Conclusions: Based on the survey, the prospect of traditional medical market is positive, but the effort of the people in traditional medical system and the systematic support from government are required to activate the stagnated traditional medical market.

Objectives and Methods: The present study was performed to investigate acupuncture point location of SI11 through reviewing literatures. Results: 1. The first description of the location of SI11 appeared in "ChimGuGabEulGyong(ZhenjiuJiayiJing)", however the point description was not precise. 2. The first proportional measurement for SI11 showed up after Ching dynasty, which describes "SI11 is the point obliquely superiour 1.7 B-cun and transeversly interior to SI9" however this does not match the present WHO's standard location of SI11. 3. The WHO standard location of SI11 is in the scapular region, in the depression between the upper one third and lower two thirds of the line connecting the midpoint of the spine of the scapula with the inferior angle of the scapula, but there is no evidence to support this in classical literatures. 4. Based on myology and theories of meridian, we suggest that it is more reasonable to locate SI11 at the point in the depression between the upper two third and lower one thirds, not the upper one third and lower two thirds, of the line connecting the midpoint of the spine of the scapula with the inferior angle of the scapula. Conclusions: More studies are needed for the more reliable standard location of SI11.

Objective: ZhangJinYoPian(藏珍要編, ZJYP) is a book witten by Song-WooGe in the late 19th century. While Korea has lost this book to tell us Korea traditional acupuncture treatment in the late 19th century, This book appeared as a manuscript in Japan. There is no reaserch or study carried out for this book. Furthermore, a few people know existence of this book. So I analyzed acupuncture treatment of the ZJYP and sort out the distinctive feature of it. Method: For that, I studied bibliographic information of this book and classified contents of it into three parts - medical thought, needle manipulation and acupoint selection to consider it's caracteristics. Results and Conclusions: 1. The author of ZJYP thought that weakness of good power in the body and strongness of evil power out of the body cause a disease. so Doctor should examine quality of both to care patient's pain. 2. The author of ZJYP thought that needle manipulation is one of the most important thing. Doctors can use needle manipulation to control patient condition. Especially, he suggested cooling and heating manipulation except reinforcement and reduction. 3. He stressed viscera and bowels. he located viscera and bowels theory in front of the book to explain relationship of these. It shows us that ZJYP maintained academic characteristics of acupuncture in Chosun-Korea. 4. While He minimized the number of acupoints used in treatment, he multiplied its combination. He selected one or two acupoints from each meridian pathway. It include Eight Confluent points(八脈交會穴). But these points were used in different ways to control the viscera and bowels, not to care the eight extra meridians.