Browse > Article

Immunomodulatory activity of cultivated wild ginseng pharmacopuncture  

Kim, Young-Jin (Dept. of Merdian & Acupoint, College of Oriental Medicine, Sangji University)
Lee, Joon-Moo (Dept. of Merdian & Acupoint, College of Oriental Medicine, Sangji University)
Lee, Eun (Dept. of Pharmaceutical Engineering, College of Health Sciences, Sangji University)
Publication Information
Korean Journal of Acupuncture / v.27, no.1, 2010 , pp. 31-47 More about this Journal
Abstract
Objectives: To investigate the anti-inflammatory effects of cultivated wild ginseng pharmacopuncture in lipopolysaccharide (LPS)-induced inflammatory rat model. Methods: Sprague-Dawley rats were divided into 4 groups; LPS control (n=6), LPS+cultivated wild ginseng pharmacopuncture at CV4 (n=6), LPS+cultivated wild ginseng pharmacopuncture at CV17 (n=6), and LPS+cultivated wild ginseng pharmacopuncture at Ex-HN1 (n=6). Pharmacopuncture (0.1 ml) was given every two days for 4 weeks followed by inflammation induction by peritoneal LPS injection (5 mg/kg). Blood, liver tissue, and peritoneal lavage fluid were taken and proinflammatory cytokines and other related factors were analysed. Results: Compared with the control group, CV4 and Ex-HN1 pharmacopuncture groups significantly attenuated plasma IL-$1{\beta}$, IL-6, and TNF-$\alpha$ increase at 2h and 5h after LPS injection (P<0.05). A significant difference from control group emerged at 5 h for plasma IL10 (P<0.05). For liver cytokines analyzed at 5 h after LPS injection, only CV4 pharmacopuncture group showed significant difference in TNF-$\alpha$ and IL-10 (P<0.05). Blood CD4/CD8 ratio and the phagocytic activities of polymorphonuclear neutrophils were not different from those of control group in all pharmacopuncture groups (P>0.05). CV4 pharmacopuncture significantly attenuated increase of plasma ${NO_3}^-/{NO_2}^-$, Intracellular adhesion molecule-1 (ICAM-1), cytokine-induced neutrophil chemoattractant-1 (CINC-1), and prostaglandin $E_2$ ($PGE_2$) compared with the control group (P<0.05). Monocyte chemoattractant protein-1, $PGE_2$, and CINC-1 level of CV4 pharmacopuncture group was significantly different from those from the control group (P<0.05). Conclusions: These results indicate that cultivated wild ginseng pharmacopuncture at CV4 may have a potent anti-inflammatory effect in an LPS-induced inflammatory rat model.
Keywords
pharmacopuncture; cultivated wild ginseng; anti-inflammation; CV4;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Chiu, W. C., Tsou, S. S., Yeh, C. L., Hou, Y. C., Yeh, S. L.. Effects of $\omega$-3 fatty acids on inflammatory mediators and splenocyte cytokine mRNA expressions in rats with polymicrobial sepsis. Nutrition. 2008 ; 24 : 484-91.   DOI   ScienceOn
2 Bressan E de Queiroz Cunha F, Tonussi, C. R.. Concentration of TNF-alpha, IL-1 beta and CINC-1 for articular incapacitation, edema and cell migration in a model of LPS-induced reactive arthritis. Cytokine. 2006 ; 36 : 83-9.   DOI   ScienceOn
3 Bohmer RH, Trinkle LS, Staneck JL. Dose effects of LPS on neutrophils in a whole blood flow cytometric assay of phagocytosis and oxidative burst. Cytometry. 1992 ; 13 : 525-31.   DOI   ScienceOn
4 Aono, K., Isobe, K., Kuichi, K., Fan, Z., Ito, M., Takeuchi, A.. In vitro and in vivo expression of inducible nitric oxide synthase during experimental endotoxemia : involvement of other cytokines. J cell Biochem. 1997 ; 65 : 349-58.   DOI   ScienceOn
5 Hogg, N.. Free radicals in disease. Seminar in Reproductive Endocrinology. 1998 ; 16 : 241-8.
6 Tompson, K. C., Trowern, A., Fowell, A., Marathe, M., Haycock, C., Arthur, M. J. P. et al. Primary rat and mouse hepatic stellate cells express the macrophage inhibitor cytokine interleukin-10 during the course of activation in vitro. Hepatology. 1998 ; 28 : 1518-24.   DOI   ScienceOn
7 Weber, C.. Novel mechanistic concepts for the control of leukocyte transmigration : specialization of integrins, chemokines, and junctional molecules. J Mol Med. 2003 ; 81 : 4-19.   DOI
8 Lennaed, T. W. J., Shenton, B. K., Borozotta, A., Donnelly, R. K., White, M., Gerrit, L. M., et al. The influence of surgical operations on components of the himan immune system. Br J Surg. 1985 ; 72 : 771-6.   DOI   ScienceOn
9 Harris, S. G., Padilla, J., Koumas, L., Ray, D., Phipps, R. P.. Prostaglandins as modulators of immunity. Trends in Immunology. 2002 ; 23 : 144-50.   DOI   ScienceOn
10 Moncada, S., Palmer, R. M. J., Higgs, E. A.. Nitric oxide physiology, pathophysiology and pharmacology. Pharmacological Reviews. 1991 ; 43 : 109-42.
11 Chamulitrat, W., Blazka, M. E., Jordan, S. J., Luster, M. I., Mason, R. P.. Tumor necrosis factor-$\alpha$ and nitric oxide production in endotoxin-primed rats administered carbon tetrachloride. Life Sci. 1995 ; 24 : 2273-80.
12 Louis, H., LeMoine, O., Peny, M. O., Quertinmont, E., Fokan, D., Goldman, M., et al. Production and role of interleukin-10 in concanavalin A-induced hepatitis in mice. Hepatology. 1997 ; 25 : 1382-9.   DOI   ScienceOn
13 Harbrecht, B. G., DiSilvio, M., Demetris, A. J., Simmons, R. L., Billiar, T. R.. Tumor necrosis factor-$\alpha$ regulates in vivo nitric oxide synthesis and induces liver injury during endotoxemia. Hepatology. 1994 ; 20 : 1055-60.   DOI   ScienceOn
14 Hamada, E., Nishida, T., Uchiyama, Y., Nakamura, J., Isihara, K., Kazuo, H.. Activation of Kupffer cells and caspases-3 involved in rat hepatocyte apoptosis induced by endotoxin. J. Hepatol. 1999 ; 30 : 807-18.   DOI   ScienceOn
15 Moreira, A. L., Sampaio, E. P., Zmuidzinas, A., Frindt, P., Smith, K. A., Kaplan, G.. Thalidomide exerts its inhibitory action on tumor necrosis factor alpha by enhancing mRNA degradation. J Exp Med. 1993 ; 177 : 1675-80.   DOI   ScienceOn
16 Schauder, P., Rohn, U., Schafer, G., Korff, G., Schenk, H. D.. Impact of fish oil-enriched total parenteral nutrition on DNA synthesis, cytokine release and receptor expression by lymphocytes in the postoperative period. Br J Nutr. 2002 ; 87 : S103-10.   DOI   ScienceOn
17 변재영, 손인철, 엄태식. 足三里穴 및 關元穴의 穴性에 관한 문헌적 고찰. 대한침구학회지. 1992 ; 9(1) : 173-8.
18 전국한의과대학 경락경혈학 교재편찬위원회. 大學經絡經穴學各論(下). 4판. 원주: 의방출판사. 2009 : 1137-46, 1192-7, 1312.
19 양희태, 김갑성. 膻中및 石門穴을 이용한 心包.三焦의 침구학적 임상응용에 관한 연구. 대한침구학회지. 1996 ; 13(1) : 165-73.
20 장석근, 강재희, 임윤경, 이현, 이병렬. 中脘및 關元에 시술한 천문동 약침이 항암 및 면역작용에 미치는 영향. 대한침구학회지. 2003 ; 20(5) : 159-71.
21 김종우, 황의완. 홧병환자의 한의학적 치료에 대한 임상적 연구. 대한한의학회지. 1998 ; 19(2) : 5-16.
22 황현서, 박동석. 鼻淵의 침구치료에 관한 문헌적 고찰. 대한침구학회지. 1996 ; 13(1) : 191-202.
23 김현아, 정지천. 알레르기성 비염에 대한 문헌적 고찰. 한방안이비인후피부과학회지. 1994 ; 7(1) : 53-84.
24 서주원, 채우석. 急驚風의 병인병기 및 침구치료에 대한 문헌적 고찰. 대전대학교 한의학연구소 논문집. 1995 ; 3(2) : 231-44.
25 박지수, 금윤희, 류동열. 小兒驚風의 침구치료에 대한 문헌적 고찰. 대전대학교 한의학연구소 논문집. 2001 ; 10(1) : 471-82.
26 Lindermann RA, Economou JS, Rothermel H. Production of interleukin-1 and tumor necrosis factor by human peripheral monocytes : Activated by peridontal bacteria and extracted lipopolysaccharides. J. Dent. Res. 1988 ; 67 : 1131-5.   DOI   ScienceOn
27 Sang, H., Wallis, G. L., Stewart, C. A., Yashige, K.. Expression of cytokines and activation of transcription factors in lipopolysaccharide-administered rats and their inhibition by phenyl N-tert-butylnitrone (PBN). Arch Biochem Biophys. 1999 ; 363 : 341-8.   DOI   ScienceOn
28 DeCicco LA, Rikans LE, Tuor CG, Hornbrook KR. Serum and liver concentrations of tumornecrosis factor $\alpha$ and interleukin-1 $\beta$ following administration of carbon tetrachloride to male rats. Toxicol Lett. 1998 ; 98 : 115-21.   DOI   ScienceOn
29 Simpson, K. J., Lukacs, N. W., Colletti, R. M., Kunkel, S. L.. Cytokines and the liver. J Hepatol. 1997 ; 27 : 1120-32.   DOI   ScienceOn
30 Harry, D., Anand, R., Holt, S., Davies, S., Marley, R., Fernando, B.. Increased sensitivity to endotoxemia in the bile duct-ligated cirrhotic rat. Hepatology. 1999 ; 30 : 1198-205.   DOI
31 Lindermann RA, Economou JS. Actinobacillus actinomycetemcomitans and Bacteroides gingivalis activate human peripheral monocytes to produce interleukin-1 and tumor necrosis factor. J Periodontol. 1988 ; 59 : 728-30.   DOI
32 안중혁, 이명종, 김길수. 三丹田.三關과 한의학적 치료와의 상관성에 관한 고찰. 대한의료기공학회지. 2000 ; 4(1) : 179-200.
33 왕득심. 국제표준침구혈명간여석. 고등교육출판사. 1996 : 123-4.
34 Morrison DC, Cuncan Jr, Goodman SA. In vitro biological activities of endotoxine. In bacterial Endotoxin. Alan R. Liss Inc. 1985 : 81-98.
35 권순주, 정대규. 산삼, 장뇌삼, 인삼의 면역증강효과 비교연구. 동의신경정신과학회지. 2004 ; 15(2) : 89-101.   과학기술학회마을
36 Mathiak, G., Grass, G., Herzmann, T., Luebke, T., Cu-Zetina, C., Boehm, S. A.. Capase-1-inhibitor ac-YVAD-cmk reduces LPS-lethality in rats without affecting haematology or cytokine responses. Br J Pharmacol. 2000 ; 131 : 383-6.   DOI   ScienceOn
37 rabson, A., Roitt, I. M., Delves, P. J.. Really Essential Medical Immunology. Blackwell publishing Ltd, Oxford. 2005 : 1-14.
38 Chao, C. Y., Yeh, S. L., Lin, M. T., Chen, W. J.. Effects of parenteral infusion with fish-oil or safflower-oil emulsion on hepatic lipids, plasma amino acids and inflammatory mediators in septic rats. Nutrition. 2000 ; 16 : 284-8.   DOI   ScienceOn
39 Marriot, J. B., Westby, M., Cookson, S., Guckian, M., Goodbourn, S., Muller. G.. CC-3052 : a water-soluble analog of thalidomide and potent inhibitor of activation-induced TNF-$\alpha$ production. J Immunol. 1998 ; 161 : 4236-43.
40 신순식, 김경철, 최영현, 이용태, 엄현섭, 김창식. 산삼 감정 기준의 객관성. 한의학연구소 동의 한의연. 2001 ; 5 : 107-14.
41 鄒澍. 本經疏證(上). 재판. 서울 : 아티전. 1999 : 28-35.
42 전국한의과대학 본초학교실. 본초학. 4판. 서울 : 영림사. 1998 : 531-3.
43 민관식, 양재형, 최태영, 노봉래, 신동대, 박찬웅. 한국인삼사I. 초판. 서울 : 한국인삼사 편찬위원회. 2002 : 41-7.
44 권기록. 정맥 주입용 산삼약침이 인체에 미치는 영향에 관한 임상적 연구. 대한약침학회 학술대회 논문집. 2004 ; 7(1) : 15-26.