Objectives : The aim of this study was to show the rationale of point-selection on the methods of acupuncture and moxibustion in the Phlegm section(in the Naegyeong chapter) of the Donguibogam. Methods : First, We summarized the cause of each disease in the Phlegm section(in the Naegyeong chapter) of the Donguibogam. Then, We explained the rationale of acupuncture point-selection referring to the cause of disease, physiology of the Oriental medicine, other uses of each acupuncture points in the Donguibogam, character of each acupuncture points, flow of meridian pathways and specific acupuncture points etc. Results and Conclusions : Total 9 acupuncture points were used in the Phlegm section(in the Naegyeong chapter) of the Donguibogam. Most of acupuncture points were specific acupuncture points. But, some rationale of acupuncture point-selection were explained by the cause of disease, physiology of the Oriental medicine, other uses of each acupuncture points in the Donguibogam, flow of meridian pathways etc.

Objectives : This article aims to give guidance on the conduct and reporting of case series and audits of acupuncture, based on common problems that have arisen in the past. This type of project, and particularly the prospective case series or pre- post-intervention study, may give valuable evidence of the overall effectiveness of acupuncture-for example in different situations and in different conditions- and provides one step in the research pathway before generating an hypothesis. Results & Discussion : The project should be designed with the aim of reducing bias as much as possible. Careful and detailed planning is essential for the project to produce worthwhile results that readers can evaluate and replicate. Ethical issues should be considered and formal approval may be necessary. The patient group should be recruited systematically and baseline data obtained. The treatment given should be systematic and decisions to change or end treatment made explicit. The outcome should be measured in ways that are known to be reliable and valid. Musculoskeletal problems can be evaluated with scales for pain and confirmed by measuring one other symptom such as stiffness or one other aspect such as bothersomeness. Global change scores also provide supporting information, and the MYMOP (Measure Yourself Medical Outcome Profile) measure is popular when patients with different conditions are included. The design of questionnaires for beliefs and attitudes is a specialised area that should not be attempted without expert help. Adverse events should also be recorded. Analysis of the data and the best way of summarising and presenting the results are also discussed.

Objectives : Acupuncture restores the flow of vital energy in the body by stimulating the organ-specific meridians and enhances cardiac vagal activity and suppresses sympathetic activity. The purpose of this review is to summarize and evaluate the acupuncture studies on autonomic nervous system (ANS). Methods : We conducted a PubMed search to obtain a fair sample of acupuncture studies related ANS published in English in 2007. Each article was reviewed for study objectives and outcomes, as well as acupuncture points, experimental and control interventions. Results : We reviewed a total of 15 studies published from 1997 to 2007. In normal states, acupuncture enhanced either vagal or sympathetic tone depending on the stimulated acupuncture point sites. On the other hand, most of studies demonstrated that acupuncture restored the autonomic dysfunctions in various kinds of stressful states. Conclusions : We reviewed studies that contributed to an understanding of the autonomic mechanisms of acupuncture with the hope that this review will be of benefit to the future studies. Although the relationship between acupuncture and ANS response is still uncertain, acupuncture would be a noteworthy modality for modulating autonomic dysfunction.

Objectives : Temporomandibular joint (TMJ) balance is known to be intricately integrated with nervous system, cervical spine, and meridian system balance. This retrospective study with one-group pretest-posttest design reviewed cervical spine imaging data to provide evidence of spinal alignment improving effect of TMJ balance treatment. Methods : Cervical spine imaging data including computed tomography and simple x-ray of 25 cases with painful condition were reviewed to explore any change in cervical alignment on wearing the intraoral device for TMJ balance treatment of functional cerebrospinal therapy. Results : Cervical spine alignment significantly improved on wearing the intraoral device. Conclusions : TMJ balance treatment improves cervical spinal alignment, which may be a firm basis to proceed with further research of TMJ balance therapy as a way of balancing the whole-body meridian system.

Objection : A medicine which is patient-initiative and preventive is required in Korea, which is going to an aging society. On this, we started this study to make a new rule to diagnose and treat more definitely by linking traditional Oriental medicine to Sasang Constitional medicine, which thought to be suitable because of Sasang Constitional medicine has been valued on staying, diet and human's nature which means preventive medical propensity. Methods : On this study, we discriminated Sasang Constition of 161 students, who is in college of oriental medicine, Wonkwang university, by using QSCCII program; and in those students, we checked 30 volunteers's five vicera's Back-su point on riging and depression, tenderness reaction, and color reaction after cupping treatment. Results : As a result of study of riging and depression of five vicera's Back-su point, we could confirm that defecient organ's Back-su point were more depressed than excess organ in Seum-in, Taeeum-in, Soyang-in. As a result of study of tenderness reaction of five vicera's Back-su point, we could confirm that excess organ's Back-su point showed stronger tenderness reaction than defecient organ in Seum-in, Taeeum-in; and excess organ's Back-su point showed weaker tenderness reaction than defecient organ in Soyang-in. As a result of study of color reaction after cupping treatment of five vicera's Back-su point, we could confirm that excess organ's Back-su point showed stronger color reaction than defecient organ in Seum-in, Taeeum-in; and excess organ's Back-su point showed weaker color reaction than defecient organ in Soyang-in. Those data suggest that there are close correlation between Sasang Constition and on riging and depression, tenderness reaction, and color reaction after cupping treatment of five vicera's Back-su point in treditional Oriental medicine. Conclusions : From this study, we can make a new rule to differentiate Sasang Constition by clearfy a relation between Sasang Constition and five vicera's Back-su point; and with this rule, we could contribute to popularization and objectification of Sasang Constition medicine and further more, Oriental medicine by making a Sasang Constition diagnosis instrument. With this, a trait of Sasang Constition medicine that is preventive and valued on character of individuals will contribute to building a patient-initiative and preventive medical system and will expend the domain of Oriental medicine.

Objectives : The repeatability and reproducibility of MIR-2, a newly developed impedance measurement device (four electrode method) on skin are of the meridian system, is evaluated and a method to improve the reliability is discussed. Methods : Multiple gage R&R studies were conducted for the impedance measurements over bilateral KI3 acupoint in ten participants by three assessors using MIR-2 device. Gage R&R studies were repeated after controlling the acupoint locating method or one value correction by replacing one assessor's outlying value with an average of the other assessors' values to explore any feasibility of improvement of measurement reliability. Results : Controlling acupoint locating method and replacing one value with an average of other assessors' value led to improved variation metrics in a gage R&R study. Conclusions : Measurement reliability can be improved by controlling measurement procedures or by using repeated measurement method, which will facilitate development of clinically applicable measurement device with reliability.

Objectives : Intelligent thermo-acupuncture system (ITA-system) is invented for the convenient operation of thermo-acupuncture therapy. The aim of this study is to investigate the safety of 100 ㎑ electromagnetic field (EMF) made by ITA-system. Methods : We analysed the influence of this EMF on animals. In order to do experiment in the influence of EMF, male ICR mice were exposed to EMF at different distances from the ITA-system. And we examined weight, diet, behavior, organ and hematological changes. Results & Conclusions : According to the animal studies with mice about EMF influence, there was no significant difference between the control group and the exposed groups about them. In conclusion, the safety of 100 ㎑ EMF made from ITA-system through this animal research was checked.

Objectives : Standardization and clear description of locating acupuncture points guarantees reproducibility and credibility of the results in animal studies of acupuncture points and meridians. In this study, we aimed at analyzing the acupoint description of the previous experimental studies and suggesting guidelines for related journals. Methods : We searched animal studies where acupuncture points were used in Journal of Korean Acupuncture & Moxibustion Society and Journal of Meridian & Acupoint with a limitation of period, from 2002 to 2006. Data on experimental animals, acupuncture points and their descriptions were extracted and put in a predefined form. They were classified as one of the 4 classes; A) descriptions of the acupuncture points present, B) no description of the acupuncture points and only reference provided, C) neither description of the acupuncture points nor reference provided, or D) acupuncture point detector used. Two authors independently extracted and classified the data and the disagreements were resolved by discussion. Experts were invited to rate acupuncture point description validity on a numeric rating scale in the 11-item questionnaire. Results : Two hundred and five studies were identified and classified as follows; A) 32.2% (n=66), B) 7.8% (n=16), C) 51.2% (n=105), and D) 8.8% (n=18). Experts ratings were significantly lower for class C (mean${\pm}$SD, 2.14${\pm}$1.35, 95% CI [1.90, 2.39], P = 0.004 compared with class B, P ${\leq}$ 0.001 compared with class A or C) and no difference was found between classes A, B, and D. Conclusions : Reporting of acupuncture points in experimental animals is poor and validity and reliability of the result from such reporting is hampered. We suggest that journal editors and experts provide guidelines for reporting animal acupuncture points and adopt them into guide for authors for related journals.

Objectives : Epilepsy is one of the most common serious brain disorders that affect people of all ages, and it is characterized by recurrent unprovoked seizures. We examined whether acupuncture can reduce both the incidence of seizures and hippocampal cell death in dentate gyrus (DG) using a mouse model of kainic acid (KA)-induced epilepsy. Methods : ICR mice ($20{\sim}25$ g) were given acupuncture once a day at acupoint HT8 (sobu) bilaterally during 2 days before KA injection. After an intracerebroventricular injection of 0.1${\mu}g$ of KA, acupuncture treatment was subsequently administered once more (total 3 times), and the degree of seizure was observed for 20 min. Three hours after injection, we confirmed the neural cell death using cresyl violet staining and silver impregnation staining, and determined the expressions of c-Fos and glutamate decarboxylase (GAD)-67 using immunohistochemistry techniques in the DG. Results : KA induced epileptic seizure, neural cell death, increased c-Fos expression and decreased GAD-67 expression in the DG. Acupuncture treatment at HT8 reduced the severity of the epileptic seizure and inhibited neural cell death from KA. In addition, acupuncture normalized the expressions of c-Fos and GAD-67 in the same areas. Conclusions : These results demonstrated that acupuncture treatment at HT8 may reduce the KA-induced epileptic seizure and neural cell death in the DG possibly by normalizing c-Fos expressions and the gamma-aminobutyric acid neurons.

Objectives & Methods : The purpose of this study is to observe the effects of Coicis Semen Herbal-acupuncture solution (CS-HAS) at the Joksamni (ST36) on the collagen-induced arthritis in the DBA/1J mouse. The author performed several experiments to analyze the effects of CS-HAS on arthritis; change of the weight; the spleen size and adhesion rate; serum cytokine levels; serum antibody levels; changes of immunocyte counts; the histological changes of joint. Results : In the Coicis Semen Herbal-acupuncture (CS-HA), arthritis index, the incidence of arthritis, and the degree of joint edema were decreased. In CS-HA, there was no weight loss. The size of the spleen, adhesion rate, and the edema and transformation of joint were lowered. In CS-HA, the levels of IL-1${\beta}$, IL-6, TNF-${\alpha}$, IFN-${\gamma}$, IgG, IgM, and anti-collagen II in serum and the levels of IFN-${\gamma}$, IL-4, IL-10 in spleen were significantly decreased. In CS-HA, the expression ratios of $CD45^+$ to $CD3e^+$ and $CD8^+$ to $CD4^+$ were decreased. Also, the overall $CD4^+/CD8^+$ cell ratio was lowered in spleen. Ratios of the $CD4^+/CD25^+$, $CD45^+/CD69^+$ cells were decreased in lymph nodes. In addition, ratios of the $CD3^+/CD69^+$, $CD11b^+/Gr-1^+$ cells were also decreased in synovium. In the histological study, the cartilage destruction and synovial cell proliferation, and collagen fiber destruction were decreased with CS-HA treated group. Conclusions : From the results mentioned above, it is suggested that CS-HA at the ST36 has several significant effects on the collagen-induced arthritis.

Objectives : The purpose of the present study is to evaluate the effects of herbal acupuncture (HA) with Moxi-tar for the treatment to intestinal disease in mice with 2, 4, 6 - trinitrobenzenesulfonic acid (TNBS) induced colitis. Methods : Mice were administered with 5% TNBS at day 1 and day 7. To investigate effects of HA with Moxi-tar at LI11, treatments were carried out at day -1, day 1, day 3, day 5, and day 7. It was checked on the weight and width of colon, diarrhea, edema, survival rate, changes of body weight, and myeloperoxygenase (MPO) activity. Furthermore, we carried out immunohistochemical staining and Western blot and analyzed mRNA expression by RT-PCR. Results : HA of Moxi-tar at LI11 in preventive mode suppressed macroscopic damages and damages of intestinal epithelial cells and infiltration of immune cells in the colon by TNBS. HA in early and preventive mode ameliorated various symptoms by TNBS. TNBS injection increased MPO activity in colon while HA in preventive mode suppressed increase of MPO activity. HA down-regulated NF-kB activity and reduced expression of TNF-a, IL-1b, and ICAM-1 in colon of TNBS treated mice. Similar to experiment at colon, HA down-regulated NF-kB activity and reduced expression of TNF-a, IL-1b, and ICAM-1 by TNBS in mesenteric lymph node. HA in therapeutic mode suppressed errosion and shortening of colon and MPO activity by TNBS and suppressed mRNA expression of TNF-a, IL-1b, and ICAM-1 in the colon. Conclusions : This study demonstrates that HA with Moxi-tar at LI11 represents a potential therapeutic method of inflammatory bowel diseases.

Objectives : The purpose of this study was to examine the anti-allergic effect in vivo, and to observe single toxicity in mice of Armeniacae Semen herbal acupuncture solution (ASHA). Methods : We investigated anti DNP IgE-mediated passive cutaneous anaphylaxis in rodents and compound 48/80-induced active systemic anaphylatic shock in mice after treatment at both BL13 with ASHA of 25 ${\mu}{\ell}$(mice) or 50 ${\mu}{\ell}$(rats) 3 times for 5 days. To ascertain safety and toxicity of ASHA, we examined single toxicity test. In single test, three groups were treated with different dosages of ASHA (ASHA250, ASHA500 and ASHA1000) according to on Korean Food and Drug Administration, respectively. We observed attentively motality, abnormal clinical sign, body weight change, and organ weight of mice after ASHA treatment. Results : ASHA inhibited passive cutaneous anaphylaxis and active systemic anaphylatic shock by oral administration. During toxicity experiment period, there was no difference in body weight change, and organ weight among different dose groups. Death were not found in single test i.p. group. (ASHA250, ASHA500 and ASHA1000). Several individuals of single test i.p. group were observed yellow brown discharge around anus in early period after administration. Conclusions : These results indicate that ASHA have inhibition effects on passive cutaneous anaphylaxis and active systemic anaphylatic shock, and suggest that has some toxicity in high dosage.

Objective : Oriental medicines have been applied to Membranous nephropathy(MN) for the purpose on increasing renal blood flow and modulating immune activity in according to Oriental medicine theories. Magsungsinyeom-bang (MSSYB) is one of the prescription which is known to show positive results in clinic with lack of laboratorial evidence. Thus, this study was aimed to evaluate the effects of MSSYB and partially investigate the mechanisms of it. Methods : The effect of MSSYB was evaluated by the morphology for the GBM thickening, protein excretion in urine and biochemical parameters in serum using cBSA-induced MN mice model. Mice were administered with MSSYB(250 or 500 mg/kg) or PBS for control group from experimental week 3 for 4 weeks. Results : 24 hrs proteinuria and the concentrations BUN was significantly decreased in the MS groups compared to the control group while the concentrations of serum albumin was higher in the MS groups than control group. MSSYB didn't affect the ratio of CD3e+/CD or 19CD4+/CD8 in the spleen and kidney, but inhibit the expression of IL-1${\beta}$, TNF-${\alpha}$, IL-6, and production of IgG and IgM. In histological analysis of kidney tissue, thickening of GBM was significantly decreased in the MS group compared to control group. Conclusions : MSSYB showed the positive results on the cBSA-induced membranous nephropathy in mice, thus, it could be a useful candidate for oriental drug for treating the membranous nephropathy in clinic.

Objevtives & Methods : Effects of cultivated wild ginseng pharmacopuncture at BL18 and LI11 on lipid composition, cytokine level, liver function, anti-oxidative capacity and histological characters were investigated in diet-induced obese rats. Forty male Sprague-Dawley rats weighing about 400g were divided into 4 groups of control, BL18, LI11 and BL18 plus LI11 pharmacopuncture groups and raised for 4 weeks. Results : 1. Plasma ${\beta}$-lipoprotein, free fatty acids level and TNF-${\alpha}$ levels significantly decreased in the pharmacopuncture groups compared to those of no treatment group. Plasma and liver total cholesterol, triglyceride, glucose and thiobarbituric acid reactive substance (TBARS) levels were also significantly lower than those of no treatment group. There was, however, no difference in TBARS level among pharmacopuncture groups. Liver total cholesterol level of BL18 pharmacopuncture group was lower than those of the other two pharmacopuncture groups. In LDL-cholesterol level, BL18 pharmacopuncture and BL18 plus LI11 pharmacopuncture groups only had significantly lower levels than that of no treatment group. 2. There was no significant difference between cultivated wild ginseng pharmacopuncture groups and no treatment group in IL-6, alanine transaminase (ALT), aspartic acid transaminase (AST) levels. 3. Compared with \ those of no treatment group, pharmacopuncture groups had significantly higher levels of HDL-cholesterol, superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase activities. There was, however, no significant difference among pharmacopuncture groups. 4. Histological characters of heart, kidney and liver of BL18 pharmacopuncture group were similar to those of normal rats. Conclusions : These results indicate that cultivated wild ginseng pharmacopuncture at BL18 and LI11 may suppress adipose tissue mass and lipid peroxidation and activate antioxidant system.

Objectives : We studied Effects of Hagocho(prunella vulgaris L.), Gamgook(chrysanthemum indicum L.) and Galgeun(pueraria Radix) aqua-acupuncture on the hyperlipidemic rat. methods : We investigated lowering lipid effect, oxidative capacity, concentration of TNF-${\alpha}$, IL-6, Leptin and histological consideration in hyperlipidemic rat. Forty male Sprague-Dawley rats weighing about 400g were divided into 5 groups of control, Ⅰ: Hagocho (prunella vulgaris L.)+Gamgook (chrysanthemum indicum L.) and Gokji aqua-acupuncture, Ⅱ: Hagocho(prunella vulgaris L.)+Gamgook (chrysanthemum indicum L.) and Joksamri aqua-acupuncture, Ⅲ: Hagocho(prunella vulgaris L.)+Galgeun(pueraria Radix)and Gokji aqua-acupuncture and Ⅳ: Hagocho (prunella vulgaris L.)+Galgeun(pueraria Radix) and Joksamri aqua-acupuncture. Results : Contents of plasma ${\beta}$-lipoprotein, contents of IL-6, TNF-${\alpha}$ and leptin, Plasma triglyceride and glucose, plasma total cholesterol and LDL-cholesterol, liver total cholesterol, liver triglyceride, plasma and liver TBARS, free fatty acids showed a tendency to decrease in the aqua-acupuncture groups compared to those of control group. The activities of GOT and GPT showed no significantly different in all treatment groups. Values of super oxide dismutase, glutathione peroxidase and catalase activity showed a tendency to increase in the aqua-acupuncture groups. Histological consideration of heart, kidney and liver in the aqua-acupuncture groups showed slight vasodilation and fat accumulation compared to those of normal rat. Conclusions : These results indicated that prunella vulgaris L., chrysanthemum indicum L. and pueraria Radix aqua-acupuncture at gokji(LI11) and Joksamri(ST36) suppressed adipose tissue mass and lipid peroxidation and increased antioxidant system in hyperlipidemic rat.

목 적 : 본 연구의 목적은 시상하부에서 침처치에 대한 nitric oxide synthase (NOS)발현을 nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d)를 이용한 조직화학 염색법으로 관찰하였다. 실험방법 : 동물은 Balb/c (wild type) 와 Stat 4 knockout (KO) 생쥐를 사용하였다. 염증유도는 1% carrageenan 용액 (20ul/마리)을 발 뒤꿈치 표피에 주사하였고, 침 처치는 족삼리 (ST36)에 시침하였다. 침 처치 후 5시간까지 부종율을 부종측정기로 측정하였으며, 마지막으로 부종을 측정한 후 동물을 희생하여 뇌를 적출하여 고정하였다. 침에 대한 효과를 확인하기 위하여 NADPH-d 반응의 조직염색을 실시하였다. 염증유도와 그룹간의 유의성 검증은 one-way ANOVA를 사용하였다. 결 과 : 대조군인 Balb/c와 실험군인 stat4 KO 생쥐를 carrageenan으로 염증을 유도시에 대조군은 90%이상 유도된 반면, Stat4 KO 그룹은 50% 정도의 염증만이 유도되었다. 염증을 유도한 생쥐의 족삼리에 침 처치시 대조군은 1시간에서 약 40%정도 감소하였고 (P<0.05), Stat4 KO 실험군은 유의한 염증 감소율을 보이지 않았다. 시상하부의 lateral hypothalamic area (LHA)와 paraventricular nucleus (PVN)부위의 침에 대한 효과를 NADPH-d 에 양성으로 반응하는 세포수로 비교하여 다음과 같은 결과를 얻었다. (1) 대조군에서 염증 유도시 시상하부의 PVN는 NADPH-d 양성세포수가 감소하였고, LHA에서는 증가하였다. (2) 염증을 유도한 대조군에 침을 처치시 PVN은 세포수가 증가하였고, LHA에서는 감소하는 경향을 보였다. (3) 염증을 유도한 Stat4 KO 군에서는 시상하부의 PVN과 LHA부위 모두에서 NADPH-d 양성세포수가 감소하였고, 염증유도그룹에 침을 처치시 PVN과 LHA부위 모두에서 세포수가 증가함을 관찰 할 수 있었다. (4) 대조군과 실험군 모두에 salicylic acid로 비교하였더니 염증유도 효과 및 NADPH-d 세포 수에서 침 처치와 비슷한 결과를 나타내었다. 결 론 : 침은 염증을 유도한 생쥐에서 염증 감소에 유의한 효과가 있다. 염증을 유도한 Balb/c 와 Stat4 KO 생쥐에 침을 처치 시 시상하부의 NADPH-d 발현이 LHA부위와 PVN에서 서로 다르게 나타나는 것으로 나타난다. 이러한 현상은 침 효과가 시상하부의 위치에 대한 작용이 다르기 때문이라고 생각된다.