Objective: The purpose of this study is to investigate the direct effect of organizational health, as perceived by childcare teachers, on their turnover intention, and to examine the mediating effect of work-life balance on the relationship between these two variables. Methods: The subjects of this study were 345 childcare teachers working at 45 childcare centers located in Gyeonggi-do. Descriptive statistics, factor analysis, reliability analysis, and Pearson correlation analysis were performed using SPSS 21.0. Additionally, the mediating effect was verified using the PROCESS Macro, and bootstrapping was performed to confirm the significance of the indirect effect. Results: First, there was a significant negative correlation between the organizational health of childcare centers, the turnover intention of childcare teachers, and their work-life balance. Second, it was found that work-family balance completely mediated the relationship between the organizational health of childcare centers and the turnover intention of childcare teachers, while work-leisure balance and work-growth balance partially mediated the relationship between these two variables. Conclusion/Implications: This study provides essential data for examining ways to lower the turnover intention of childcare teachers by addressing both individual and organizational dimensions.