• Journal of Applied Biological Chemistry
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• 제54권3호
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• pp.159-165
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• 2011

로즈마리 추출물의 phenol 함량은 25.7 mg/g으로 매우 높게 나타났다. Helicobacter pylori 에 대한 항균효과를 측정한 결과, clear zone이 $100{sim}200{\mu}/disc$의 phenol 농도에서 각각 11, 12, 14 mm의 clear zone을 형성하여 추출물의 농도가 증가할수록 H. pylori 에 대한 저해효과가 농도 의존적으로 높아지는 것을 확인 할 수 있었다. Sephadex LH-20과 MCI-gel CHP-20 column을 이용하여 normal phase type인 $ethanol{\rightarrow}H_2O$와 reverse phase type인 $H_2O{\rightarrow}methanol$로 용액의 농도를 증가시키면서 용출한 결과 H. pylori에 대한 탁월한 저해활성을 나타낸 5종류(compound A~E)의 단일 물질로 분리하였다. 5가지 각각의 물질은 H. pylori에 대한 항균력이 미약하였으나, 2종류 이상의 다중 물질의 항균 시너지 효과에 의해 각각 10~16 mm의 clear zone을 형성하여 우수한 항균력을 나타내었으며, fast atom bombardment (FAB)-Mass, $^1H$-NMR, $^{13}C$-NMR과 IR spectrum을 사용하여 구조 동정한 결과 protocatechuic acid, coumaric acid, caffeic acid, chlorogenic acid 및 rosmarinic acid 인 것으로 확인되었다.

• Advances in Traditional Medicine
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• 제10권1호
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• pp.44-49
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• 2010

An examination of the kinetic properties of UDP-glucose, (1,3)-$\beta$-glucans (callose) synthase, from mung bean seedlings (Sorbus commixta cortex) shows that these enzymes have a complex relationship with UDP-glucose and various effectors. Fluorescence assay showed that deoxynojirimycin increased the inhibitory effect of (1,3)-$\beta$-glucan synthase in a concentration-dependent manner. The inhibitory effect of sakuranetin (34.34%) was higher than that of deoxynojirimycin (80.63%). Disk diffusion method revealed that sakuranetin inhibited the growth of Candida albicans to a 1.5 mm inhibition zone. These results suggest that sakuranetin, isolated from Sorbus commixta cortex extract, can be used as stable antifungal material.

• 동의생리병리학회지
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• 제18권2호
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• pp.575-579
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• 2004

4-Geranyloxy compound (1) has elucidated by spectroscopic analysis. This compound (1) inhibited the growth of the dermatophytic fungus Trichophyton mentagrophytes ATCC 28185, (2 mm inhibition zone at 15 ㎍/disc), cytotoxic to P388 murine leukaemia eel/lines ATCC CCL 46 P388D1, (IC/sub 50/ 1,125 ng/ml at 7.5㎍/disc) and BSC monkey kidney cell lines (100% of well at 15 ㎍/disc).

• Natural Product Sciences
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• 제18권2호
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• pp.137-140
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• 2012

The aim of this research was to investigate the industrial application of Zanthoxylum schinifolium. Antibacterial activities of the n-hexane, methylene chloride (MC), ethyl acetate, and butanol fractions of Z. schinifolium were tested against Escherichia coli, Staphylococcus aureus and Helicobacter pylori. Among the fractions, the n-hexane and MC fractions showed the stronger antibacterial activity against H. pylori, with an inhibition zone greater than 10 mm in disc assays. Further testing of bergapten and lupeol from the MC fraction of Z. schinifolium revealed the antibacterial effects against E. coli, S. aureus and H. pylori, suggesting their potential use as antibacterial agents.

• Journal of Microbiology
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• 제37권1호
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• pp.45-49
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• 1999

To investigate the mechanism of cadmium tolerance in a cadmium-resistant Azomonas agilis PY101 that produces a specific fluorescent pigment promoted by cadmium, we carried out Tn5 mutagenesis and isolated four pigment-deficient mutants. In these mutants, Ppg1, Ppg2, and Ppg3 remarkably reduced the pigment production to 15.3%, 11.2%, and 13.9%, respectively. Especially, Ppg4 mutant did not produce the pigment at all. None of the mutants grew in the presence of 1500 ppm of CdCl2 in growth medium, and they exhibited differential sensitivities to cadmium. Ppg1, Ppg2, Ppg3, and Ppg4 mutants were sensitive to 900 ppm, 1100 ppm, 1000 ppm, and 800 ppm of CdCl2, respectively. These mutants also showed noticeable increase, from 8.8-fold to 13.2-fold, in the size of growth inhibition zone compared with that of the will type after treatment with cadmium. Therefore, the pigment production of A. agilis PY101 was found to decrease the toxic effects of cadmium to the bacterium.

• International Journal of Oral Biology
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• 제46권1호
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• pp.45-50
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• 2021

This study evaluated the antimicrobial activity of Endoseal TCS, an mineral trioxide aggregate-based root canal sealer, mixed with water-soluble mangostin derivatives (WsMD) of Garcinia mangostana L. (mangosteen) ethanol extract against Enterococcus faecalis and Staphylococcus aureus. The antibacterial activity of Endoseal TCS mixed with WsMD against three strains of E. faecalis and three strains of S. aureus was performed using agar diffusion test. The data showed that Endoseal TCS mixed with 0.115% WsMD had a zone of inhibition of 0.7 ± 0.2-2.4 ± 0.1 mm. The results suggest that Endoseal TCS mixed with WsMD of Garcinia mangostana L. ethanol extract is useful as a root canal sealer with antibacterial activity against E. faecalis and S. aureus.

• Bulletin of the Korean Chemical Society
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• 제34권8호
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• pp.2441-2445
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• 2013

Chitosan non-woven fabrics were acetylated to improve their antimicrobial properties. The active chlorine content, antimicrobial properties, storage stability, and surface properties of acetylated chitosan non-woven fabrics were investigated. The active chlorine content of the fabrics increased upon reduction of the degree of the acetylation or increase in sodium hypochlorite concentration. Acetylated chitosan non-woven fabrics showed powerful antimicrobial activity by efficiently killing Escherichia coli and forming a growth inhibition zone for Staphylococcus aureus. Furthermore, scanning electron microscopy observations demonstrated that the acetylated chitosan non-woven fabrics were not damaged in sodium hypochlorite solution.

• Preventive Nutrition and Food Science
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• 제3권4호
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• pp.324-328
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• 1998

We screened the methanol extracts from 133 plant species growing in Korea for antimicrobial activity against enterohemorrhagic Escherichia coli 0157 : H7. Those are selected from three plant grouping ; traditional medicinal herbs, edible plants, and flowers. They were tested by disk diffusion assay. From evaluation of the inhibition zone diameter of microbial growth, we found that the flower extract of Rhododendron Schilpenbachii Max had the most significant antimicrobial activity against this bacteria. Extracts from most of the vegetables and plants did not show antimicrobial activity except for the leaves of Ginkgo biloba L. and seeds of Prunus Dallicina L. did not show antimicrobial activity except for the leaves of Ginkgo biloba L. and the seeds of Prunus sallicina L.

• 대한치과보철학회지
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• 제37권3호
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• pp.293-300
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• 1999

The purpose of this study was to evaluate the in vitro antifungal effect of several kinds of denture lining materials containing nystatin and silver-zeolite on Candida albicans. Three commercially available tissue conditioners (Soft-Liner, Coe-Comfort, Coe-Soft) and two direct denture relining materials (Tokuso Rebase, Durabase) were selected. In terms of the zone of inhibition and some basic physical properties, I could find the following results ; 1. Nystatin or silver-zeolite included in those relining materials had definite antifungal activities against the Candida albicans. 2. As times went on, both of the antifungal agents's activities decreased gradually. 3. Antifungal agents did not affect the relining materials' basic physical properties. 4 Direct chairside relining materials showed unfavorable dark discoloration with response to silver-zeolite.

• 한국식품영양과학회지
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• 제23권6호
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• pp.994-1000
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• 1994

Hot water extract from mugwort (Artemisia asiatica nakai) leaves and tea inhibited the growth of Bacillus subtilis by adding to the nutrient broth 1.0% and 0.5% concentration, respectively. Among the important compounds that contributing mugwort like flavor to the mugwort leaves and tea were considered, authenic compound of thujone, caryophyllene and farnesol showed bactericidal effect for Escherichia coli, Enterobacter aerogenes, Vibrio paraheaemolyticus, Psudomonas aeruginosa, Bacillus subtilis and Staphylococcus aureus when teste by paper disc method ( 8 mm diameter). The mixture of caryophyllene and farnesol was more bactericidal effect for various bacteria than the mixture of thujone, caryophyllene and farnesol was more bactericidal effect for various bacteria that the mixture of thujone, caryophyllene and farnesol or each compounds . Especially, the mixture of caryophyllene and farnesol showed strong bactericidal effect (diameter of inhibition zone>40mm) for Vibrio parahaemolyticus , Enterobacter aerogenes and BAcillus subtilis.