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http://dx.doi.org/10.3742/OPEM.2010.10.1.044

Inhibitory effect of sakuranetin on (1,3)-β-glucan synthase  

You, Myung-Ja (Division of Nanobiochemistry, College of Natural Products, Wonkwang University)
Kim, Bo-Mi (Division of Nanobiochemistry, College of Natural Products, Wonkwang University)
Bhatt, Lok Ranjan (Division of Nanobiochemistry, College of Natural Products, Wonkwang University)
Chai, Kyu-Yun (Division of Nanobiochemistry, College of Natural Products, Wonkwang University)
Baek, Seung-Hwa (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University)
Publication Information
Advances in Traditional Medicine / v.10, no.1, 2010 , pp. 44-49 More about this Journal
Abstract
An examination of the kinetic properties of UDP-glucose, (1,3)-$\beta$-glucans (callose) synthase, from mung bean seedlings (Sorbus commixta cortex) shows that these enzymes have a complex relationship with UDP-glucose and various effectors. Fluorescence assay showed that deoxynojirimycin increased the inhibitory effect of (1,3)-$\beta$-glucan synthase in a concentration-dependent manner. The inhibitory effect of sakuranetin (34.34%) was higher than that of deoxynojirimycin (80.63%). Disk diffusion method revealed that sakuranetin inhibited the growth of Candida albicans to a 1.5 mm inhibition zone. These results suggest that sakuranetin, isolated from Sorbus commixta cortex extract, can be used as stable antifungal material.
Keywords
(1,3)-$\beta$-glucan synthase; Sorbus commixta cortex; Fluorescence assay; Inhibitory effect; Sakuranetin; Candida albicans;
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Times Cited By KSCI : 9  (Citation Analysis)
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