• Ocean and Polar Research
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• 제26권3호
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• pp.475-480
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• 2004

Recently, less toxic and more effective anaesthetics are essential for marine fishes. Lidocaine belongs to a group of anaesthetics which are used as local anaesthetic in human medicine. This chemical was tested fer winter flounder, Pleunnectes americanus. Anaesthetic effect of lidocaine hydrochloride-sodium bicarbonate mixture (lidocaine $HCl/NaHCO_3$) was tested for the winter flounder at five different temperature regimes: $3^{\circ}C,\;7^{\circ}C,\;11^{\circ}C,\;15^{\circ}C\;and\;19^{\circ}C$ Anaesthetic dose and temperature-dependent relationship in exposure and recovery time were observed for the winter flounder of $17.2{\pm}0.1cm$ mean total length. Based on the results, anaesthetic lidocaine $HCl/NaHCO_3$ showed rapid exposure time and rapid recovery time for winter flounder. The results indicate that lidocaine $HCl/NaHCO_3$ can be used as suitable anaesthetic for this species.

• 한국양식학회:학술대회논문집
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• 한국양식학회 2003년도 추계학술발표대회 논문요약집
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• pp.28-28
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• 2003

Viable interspecific hybrids between yellowtail flounder (Pleuronectes ferrugineus, Store.) and winter flounder (Pleuronectes americanus, Walbaum) were produced by artificial insemination of yellowtail flounder eggs with winter flounder sperm. However, mean fertilization rate, hatching success and early survival up to 3 weeks post hatch were significantly lower than those of parental pure cross controls (P<0.01). Overall, cytogenetic traits (karyological analysis and estimation of cellular DNA contents using flow cytometry) of hybrid flounder were intermediate between the two parental species. Microsatellite assay was used to distinguish the parental genomes in the hybrids; in most cases, one allele was specific to each of the parents. Morphometrics assessed by body proportions indicated that hybrids generally displayed a morphology intermediate between the maternal and paternal species. Interspecific hybrids exhibited abnormal and retarded gonad development in both sexes based on histological analysis of gonads from adult fish. The sterility of the hybrids presents a significant advantage for their use in aquaculture, as potential escapees would not be capable of reproducing in the wild and contaminating natural stocks.

• 한국수산과학회지
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• 제44권2호
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• pp.149-154
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• 2011

Two experiments were conducted to investigate the effects of dietary moisture content on the growth and gastrointestinal evacuation of sub-adult olive flounder (Paralichthys olivaceus) in the summer and winter seasons. In the first experiment, three experimental diets containing different moisture levels (7%, 15%) and additives were prepared by adding water and commercial additives (1% nutrients, 1% digestives) to commercial extruded pellets. Fish (initial weight: $332{\pm}9.2$ g) were distributed randomly into six 3000 L tanks (25 fish/tank) in a flow-through tank system. Two replicate groups of fish were fed the experimental diets to satiation for 14 weeks during the summer season ($21.4{\pm}2.5^{\circ}C$). Survival, weight gain, feed efficiency, and daily feed intake did not differ significantly among groups. In the second experiment, two experimental diets containing different moisture levels (8%, 23%) were prepared by adding water to commercial extruded pellets. Fish (initial weight: $646{\pm}6.7$ g) were randomly distributed into six 3000 L tanks (20 fish/tank) in a flow-through tank system. Three replicate groups of fish were fed the experimental diets to satiation for 19 weeks during the winter season ($12.2{\pm}1.2^{\circ}C$). Weight gain, feed efficiency, and daily feed intake did not differ significantly among groups. Gastrointestinal evacuation rates were determined after the end of the winter feeding trials. The stomach contents of fish fed diets containing different moisture levels peaked within 3 h after feeding and then decreased gradually over 40 h to approach pre-feeding levels. Intestinal contents began to accumulate 3 h after feeding and reached a maximum at 32 h, then declined until nearly complete evacuation around 56 h. The stomach contents reached moisture levels of approximately 70% within 3 h after feeding and then increased gradually. No considerable difference in moisture levels was observed in the stomach contents of fish fed diets with different moisture contents. The results of this study suggest that the gastric evacuation of sub-adult olive flounder was not affected by dietary moisture level and the addition of water to the diet had no beneficial effect on the growth of sub-adult olive flounder in the summer and winter seasons.

• Fisheries and Aquatic Sciences
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• 제8권2호
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• pp.65-69
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• 2005

We evaluated the effects of the partial dietary replacement of fish meal with squid liver $Meal^{TM}$ on the growth and body composition of juvenile olive flounder Paralichthys olivaceus during the winter season. Twenty-five fish with an initial body weight of 23 g per tank were distributed among 12,250 L flow-through tanks. The experimental diets, which were designated SLM5, SLM1O, and SLM15 diets, were prepared in triplicate along with control diet by replacing 5, 10, and $15\%$ of mackerel fish meal with squid liver $Meal^{TM}$, respectively. The weight gain and specific growth rate of flounder that were fed the control and SLM5 diets did not differ from those of the fish fed the SLM10 diet, but they were significantly (P<0.05) higher than those of the fish fed the SLM15 diet. The feed efficiency ratios for the flounder that were fed the control, SLM5 and SLM10 diets were significantly (P<0.05) higher than for the fish that were fed the SLM15 diet. However, the protein efficiency ratio for the flounder was not significantly affected by the experimental diets. The crude protein, crude lipid and ash content, and blood chemistry of the flounder were not significantly affected by the experimental diets. Therefore, the replacement of up to $10\%$ of dietary fish meal with squid liver $Meal^{TM}$ can be made without a reduction in growth or a deterioration of the feed efficiency of juvenile olive flounder during the winter season.

• 한국양식학회지
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• 제18권1호
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• pp.31-36
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• 2005

본 연구는 겨울철 넙치 사육시 부상 배합사료의 적정 공급횟수와 공급률을 조사하기 위해 평균 체중 45.0 g의 넙치를 각 수조마다 20마리씩 3반복으로 수용하여 60일간 사육하였다. 사료 공급횟수는 1일 3회, 1일 2회, 1일 1회 및 2일 1회로 설정하였으며 사료 공급량은 만복 및 만복의 $80\%$로 설정하였다. 생존율은 모든 실험구에서 $96\%$이상이었으며, 증중량은 1일 2회 만복 공급구가 1일 3회 공급구와 통계적인 차이는 없었지만 가장 높았고. 2일 1회 제한 공급구가 가장 낮은 결과를 보였다. (P<0.05). 그리고 동일한 공급횟수에서 만복 공급구가 제한 공급구보다 높은 성장을 보였다. 일일사료섭취율은 만복 공급구와 제한 공급구 모두 공급횟수가 증가할수록 증가하다가 1일 2회 공급구 이상에서는 유의한 차이가 없었다. 사료효율과 단백질효율은 1일 3회 제한 공급구가 가장 높았고 2일 1회 제한 공급구가 낮은 값을 보였으며(P<0.05), 나머지 실험구들 사이에는 유의한 차이를 보이지 않았다. 사육 실험 종료시 전어체의 수분, 단백질, 지질 및 회분함량은 모든 실험구간에 유의한 차이가 없었다. 이상의 결과로부터, 평균체중 45-53 g의 넙치치어를 겨울철에 사육할 때는 부상배합사료를 1일 2회 만복으로 공급하는 것이 바람직 할 것이라 판단된다.

• 한국양식학회지
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• 제1권1호
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• pp.25-39
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• 1988

사육 조건하에서 넙치는 사육 수온이 $12^{\circ}C$에서 $14\~15^{\circ}C$로 상승하고 광선 주기가 10L/14D에서 14L/10D로 되는 시기에 성숙하여 산란하였다. 사육 환경하에서 사육된 어미의 산란 기간중 전반기에 산란된 수정란이 후반의 것에 비하여 양질의 알로서 종묘의 대량 생산시에는 전반의 수정란을 활용하는 것이 바람직하다.

• 한국수산과학회지
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• 제43권3호
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• pp.217-222
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• 2010

Two 15-week feeding trials were conducted to investigate how the rate and frequency of feeding affected the winter growth of olive flounder ($13^{\circ}C$). In the first experiment, triplicate groups of fish (initial mean weight $117{\pm}6.0$ g) were fed extruded pellets twice a day at feeding rates of 0.1, 0.25, 0.4, 0.55, and 0.57% (satiation) body weight per day (BW/d). The weight gain, specific growth rate, and feed efficiency increased significantly (P<0.05) with the feeding rates from 0.1 to 0.55% BW/d, but no significant differences in these parameters were found for fish fed diets of 0.55% BW/d and satiation. The moisture and ash contents of whole body of fish tended to decrease as the feeding rate increased, but the opposite trend was found for crude lipid content. In the second experiment, triplicate groups of fish (initial mean weight $117{\pm}6.3$ g) were fed extruded pellets to apparent satiation at the three different feeding frequencies: one meal 2 days, one meal a day and two meals a day. The weight gain and specific growth rate of fish fed one meal 2 days were significantly (P<0.05) lower than those of fish fed one or two meals a day, whereas no significant differences in the weight gain and specific growth rate were found between fish fed one and those fed two meals a day. Feed efficiency and condition factor were not significantly affected by feeding frequency. Based on these results, a feeding rate of 0.3% BW/d is recommended as maintenance feeding level, and the optimum feeding frequency is one meal a day with satiation feeding for the growth of olive flounder (117-147 g) during winter ($13^{\circ}C$).

• Fisheries and Aquatic Sciences
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• 제12권2호
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• pp.104-110
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• 2009

Feeding trials were performed at two different water temperatures (summer and winter seasons) to identify suitable protein and lipid (energy) levels to be used in formulating extruded pellets for olive flounder. Experiments were conducted to determine the effects of protein and lipid levels in extruded pellets on the growth and body composition of the flounder in both feeding trials. Six experimental diets were prepared containing three protein levels (46%, 51%, and 56%) and two lipid levels (10% and 17%). In the first experiment, during the summer season ($22{\pm}2.2^{\circ}C$), a triplicate group of fish (initial weight, 114 g) were fed to satiation one of the six diets for 11 weeks. The highest weight gain was observed in fish fed the 56/17 (% protein/% lipid) diet, but this weight gain was not significantly different from that of fish fed the other diets, except for those fed the 46/10 diet. The feed efficiency and protein efficiency ratio of fish fed the 17% lipid diets were higher than those of fish fed the 10% lipid diets at each protein level. In the second experiment, during the winter season ($13{\pm}1.5^{\circ}C$), a triplicate group of fish (initial weight, 107 g) were fed to satiation one of the six diets for 9 weeks. Weight gain was not significantly different among all groups. The feed efficiency and protein efficiency ratio tended to increase with increasing dietary lipid level at each protein level. The whole-body crude lipid content of the of fish fed the 17% lipid diets was significantly higher than that of fish fed the 10% lipid diets at each protein level in both feeding trials. Based on the data obtained in this study, the inclusion of dietary protein at a level of 46% appears to be sufficient to support optimal growth, and increasing the dietary lipid level from 10% to 17% had no beneficial effects on the growth and feed utilization of olive flounder (110-300 g), except for fish fed a 56% protein diet in the summer season.

• 현장농수산연구지
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• 제25권3호
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• pp.35-46
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• 2023

It was in the 1982 that artificial seed production research for olive flounder (Paralichthys olivaceus) farming was first conducted in Korea (Currently, National Institute of Fisheries Science, Fish Breeding Research Center). In 1985, fertilized eggs were obtained from natural olive flounder adapted to land tanks, and artificial seed production technology was established and fertilized eggs were distributed. In the late 1980s, halibut aquaculture began to prosper in land-based tank farming in Jeju Island and Busan's Gijang region, where water temperatures are relatively high in winter. Currently, aquaculture is being carried out all over the country, centering on Jeju Island and Wando, Jeollanam-do. However, olive flounder farming, which started with a small group in the 1980s, reduced genetic diversity through inbreeding over generations, resulting in side effects such as slow growth, reduced resistance to disease and environmental conditions. In order to solve these genetic problems of farmed olive flounder in Korea, the Fish Breeding Research Center of the National Institute of Fisheries Science introduced a wild-caught parent fish group to the existing aquaculture group from 2003 to 2004. Genetic diversity was secured and KingNupchi with fast growth and improved body shape was developed. In this study, the current status of breeding technology development of olive flounder, a major aquaculture breed in Korea, is reviewed and future research directions are suggested.

• Fisheries and Aquatic Sciences
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• 제8권3호
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• pp.138-141
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• 2005

This study was conducted to investigate the effect of partially substituting meat meal for fish meal on the growth and body composition of juvenile olive flounder Paralichthys olivaceus during the winter season. Twenty-five fish (initial body weight, 23 g) were distributed into twelve 250 L flow-through tanks. Four experimental diets were prepared in triplicate: the control, MM20, MM40, and MM60 diets. Sixty percent mackerel meal was used as the primary protein source in the control diet. Meat meal was substituted for 20, 40, and 60$\%$ of the mackerel meal in the MM20, MM40, and MM60 diets, respectively. Survival was not significantly affected by the experimental diets. However, the weight gain and specific growth rate of fish fed the control, MM20, and MM40 diets were significantly higher than those of fish fed the MM60 diet (P<0.05). The feed efficiency ratio of fish fed the control, MM20, and MM40 diets was significantly higher than that of fish fed the MM60 diet (P<0.05). The protein efficiency ratio for fish fed the control diet was significantly higher than that for fish fed the MM40 and MM60 diets (P$\%$ substitution of meat meal for fish meal in the diet could be implemented without a reduction in growth or deterioration of the feed efficiency of juvenile olive flounder during the winter season.