• 생명과학회지
• /
• 제20권7호
• /
• pp.999-1004
• /
• 2010

밀기울 발효에서 효소회수의 모델로 Aspergillus niger를 고체상태로 발효시켜 조사하였다. 발효시킨 밀기울에서 증류수로 효소추출 효율은 초산 완충액, 구연산 완충액, 구연산-인산 완충액 및 5% 메탄올 처리보다 높았다. 따라서, 추출 용매로 증류수를 이용하여 최적 조건을 상세히 검토하였다. 최적 조건은 고체와 액체 용매를 1:5의 비율로 증류수로서 세 번 세척하였을 때에 최대 회수율을 0.025 U/g으로 확보하였다.

• 한국환경농학회지
• /
• 제22권4호
• /
• pp.290-293
• /
• 2003

To measure an effects of strontium on secretion of ${\alpha}$-amylase and RNase, wheat aleurone layers were isolated after the pre-incubation in a solution with or without 10 mM $SrCl_2$ or $CaCl_2$ for 3 days at $25^{\circ}C$ in the dark under aseptic conditions. The secretion of ${\alpha}$-amylase reached a maximum at 72 h after incubation. $Sr^{2+}$ induced more effectively secretion of ${\alpha}$-amylase than $Ca^{2+}$. The ${\alpha}$-amylase secretions by $Sr^{2+}$ or $Ca^{2+}$ ware about $2 (Ca^{2+})$ to $2.5 (Sr^{2+})$ fold higher than it without divalent ions, When aleurone layers were incubated without divalent ions, however, the ${\alpha}$-amylase was remarkably retained in the tissues. Total ${\alpha}$-amylase synthesis (ie. tissues + media) was slightly lowered by 10mM $SrCl_2$ addition. It seemed that the RNase secretion begins at 18 h after incubation. This meaned that the RNase secretion may process slower than ${\alpha}$-amylasee secretion. $Ca^{2+}$ effect on RNase secretion is stronger than $Sr^{2+}$ unlikely to ${\alpha}$-amylase. The secretion process is likely to be suddenly induced between 72 hand 96 h. These results suggested that the secretion was enhanced after the accumulation in aleurone layers.

• 한국축산식품학회지
• /
• 제37권3호
• /
• pp.410-417
• /
• 2017

The effect of dietary fiber enrichment (wheat bran) and cooking methods (oven, steam and microwave) on functional and physico-chemical properties of raw nuggets formulation as well as nutritional, color and textural properties of chicken nuggets were analyzed in this study. Among different cooking methods used for nuggets preparation, steam cooked nuggets had significantly (p<0.05) higher water holding capacity (56.65%), cooking yield (97.16%) and total dietary fiber content (4.32%) in comparison to oven and microwave cooked nuggets. The effect of cooking methods and wheat bran incorporation was also noticed on textural properties of the nuggets. Hardness, firmness and toughness values of oven and steam cooked nuggets were significantly (p<0.05) higher than microwave cooked nuggets. Among nuggets prepared by different cooking methods, cohesiveness of microwave cooked nuggets was found to be significantly (p<0.05) highest, whereas, oven cooked nuggets had significantly (p<0.05) highest gumminess and chewiness values. Steam cooked nuggets were found to be better among all nuggets due to their higher cooking yield and dietary fiber content.

• 한국식품과학회지
• /
• 제31권1호
• /
• pp.138-146
• /
• 1999

한국산 밀 우리, 은파, 올그루, 그루, 탑동 5 품종과 호주산 연질 밀(ASW), 미국산 연질 밀(WW)과 경질 밀(DNS)로 국수를 제조한 후 조리특성, 기계적 및 관능적 조직감 특성을 비교 검토하였으며 밀가루의 이화학적 성질과의 연관성을 살펴보았다. 국수의 조리 손실율은 밀가루의 단백질 함량이 높을수록 낮고, 수분흡수율은 손상전분의 함량이 높을수록 컸다. 측정 방법을 달리한 기계적 특성에서는 견고성, 씹음성, 인장력 등이 경질 밀인 탑동밀과 DNS가 연질 밀보다는 높은 값을 보이고, 외국산 연질 밀인 ASW, WW가 한국산 밀들보다 낮은 값을 나타냈다. 관능적 특성과 기계적 특성과의 상관관계를 맺어본 결과 multi-blade compression shear test가 10% 압착시험이나 반복 압착시험 및 인장력 시험보다도 더 나은 관계를 보였으며, 표면굳기는 multi-blade compression shear test의 면적과 가장 나은 상관관계를 보였고(r=0.76) 내부굳기와 씹음성은 기울기와 잘 연관되었다. 국수의 관능적 특성과 밀가루의 물리화학적 특성과의 상관관계에서는 단백질의 함량이 국수의 조직감에 가장 큰 영향을 미쳤다. 아밀로즈의 함량은 국수의 유연성 및 미끄럼성과 양의 관계를, 표면굳기, 내부굳기, 씹음성과는 음의 관계를 보였다. 삶은 국수의 색도는 외국산 밀(WW, ASW)에 비해 한국산 밀의 국수가 더욱 더 어두운 색을 띄었다. 기호도 검사 결과, 한국산 연질밀인 그루밀이 수입밀인 WW 및 ASW보다 더 선호하는 것으로 나타났다.

• 한국축산식품학회지
• /
• 제37권1호
• /
• pp.52-61
• /
• 2017

The objective of this study was to investigate the effects of different concentrations of WSP (wheat sprout powder) and ISP (isolated soy protein) on the quality of breakfast sausage. Treatments were formulated as follows: Control, T1 (2.0% ISP), T2 (1.5% ISP + 0.5% WSP), T3 (1.0% ISP + 1.0% WSP), T4 (0.5% ISP + 1.5% WSP) and T5 (2.0% WSP). The treatments were analyzed for color, pH, cooking loss, emulsion stability, protein solubility, viscosity, texture properties and sensory evaluation. Lightness and redness were reduced and yellowness was increased as increased level of WSP, due to the dark green color of WSP (p<0.05). The pH values of all samples were affected by WSP which has lower pH (p<0.05). The emulsion stability and cooking loss of treatments were improved with increasing level of WSP (p<0.05). The protein solubility, viscosity and overall texture properties of the sausage indicated significant differences in relation to the level of WSP and ISP (p<0.05). The sensory evaluation indicated that the greatest flavor and overall acceptability in sausage was achieved at WSP 1% combination with ISP 1% (T3) (p<0.05). Therefore, these results indicate that breakfast sausage containing 1% WSP and 1% ISP is the optimal formulation, taking into consideration the overall physico-chemical properties and sensory evaluation.

• Journal of Forest and Environmental Science
• /
• 제31권4호
• /
• pp.297-302
• /
• 2015

With the intention to solve environmental problems caused by synthetic plastics from petroleum resources, biodegradable polyurethane foams and thermosetting moldings were prepared from biomass, such as wood and wheat bran by liquefaction method. Biodegradability of these biomass-based polymeric materials was investigated. In activated sludge, polyurethane foams from liquefied wheat bran and thermosetting molding from phenolated wood were decomposed approximately 14% and 29% for 20 days, respectively. One of the wood fungi, Coriolus versicolor was able to grow without supplemental nutrition, only with distilled water and polyurethane foam as a nutrition source. Risk assessments were also conducted and results showed that estrogenicity, mutagenicity, and carcinogenicity were not observed in the extractives of biomass- based polymeric materials.

• 한국식품과학회지
• /
• 제5권1호
• /
• pp.16-24
• /
• 1973

국산원료(國産原料)를 활용(活用)한 복합분(複合粉)으로 빵을 만들고 그들의 품질(品質)을 조사한 바 다음과 같은 결과를 얻었다. 1) 보리빵의 제조(製造)에 있어서, 표준 밀가루빵(speciffic loaf volume, SLV : 3.3)과 동일(同一)한 formula로 제조(製造) 할 때, 그 SLV 는 1.3(물 100%)으로 아주 낮았으나 탈지대두분(脫脂大豆粉)을 10% 혼합(混合)하면 1.7(물 100%)로 증가하였다. 한편 분질개량(粉質改良)을 위한 여러 가지 첨가제중(添加劑中)에서 1.5% GMS + 0.5% CSL 의 경우가 가장 우수하였다. (SLV : 2.0, 물 100%) 이에 다시 밀가루를 혼합(混合)하면 가장 효과적(效果的)이었는 바, 보리가루의 25%를 밀가루로 대체하면 그 SLV 는 2.8(물 90%)로, 50%의 경우는 3.2(물 90%)로 증가하여 표준빵과 비슷하였으며, 빵색깔도 우수하였다. 2) 고구마빵은 baking이 끝나면 흑갈색으로 되는것이 특징(特徵)이고 탈지대두분(脫脂大豆粉) 20%를 혼합(混合)하면 그 loaf volume이 증가하며 결착제(結着劑)로서 GMS + CSL의 첨가(添加)는 효과적(效果的)이었다. (SLV : 1.9, 물 100%) 고구마가루 복합분에 밀가루를 25%와 50%수준(水準)으로 혼합(混合)하면 그 SLV는 각각(各各) 2.3, 2.6(물 90%)으로 증가하고 빵색깔이 연해지며 빵조직(組織)이 개선되었다. 3) 감자가루를 주원료(主原料)로 한 제빵은 그 분질(粉質)이 고구마가루와는 다르고 GMS + CSL의 첨가(添加) 효과가 낮았다. 옥수수가루에 의한 제빵은 쌀보리가루와는 상이한 경향이었으며, 탈지(脫脂)쌀겨가루는 제빵 원료(原料)로서 부적당하였다. 4) 원료(原料) 공급(供給)면에서 전망(展望)이 보이는 쌀보리가루와 고구마가루를 주원료(主原料)로 한 5종(種)의 복합분(複合粉), 즉 (1) 쌀보리가루+탈지대두분+밀가루 (45 : 10 : 45) (2) 쌀보리가루+탈지대두분+밀가루 (67.5:10:22.5) (3) 쌀보리가루+탈지대두분 (90 : 10) (4) 고구마가루+탈지대두분+밀가루 (40 : 20 : 40) (5) 고구마가루+탈지대두분+밀가루 (60 : 20 : 20) 으로 첨가제를 넣고 제빵한 후, 밀가루빵을 표준(So)으로 하여 관능시험(官能試驗)한 결과(結果), 평균점(平均點)의 서열은 So>(1)>(2)>(3)>(5)>(4) 이었고, Duncan's multiple range test 의 결과 (1)의 빵, 즉 보리가루와 밀가루를 동량(同量)섞고 여기에 탈지대두분(脫脂大豆粉)을 10% 혼합한 복합분(複合粉)을 주원료(主原料)로 한 빵은 그 색깔, 조직(組織), 및, 향기(香氣)등의 종합평가(綜合評價)에 있어서 밀가루빵과 유의차(有意差)가 없었다.

• 한국미생물·생명공학회지
• /
• 제43권4호
• /
• pp.378-384
• /
• 2015

누룩은 전통주를 빚기 위한 발효제이다. 본 연구의 목적은 누룩 발효시 온도의 영향을 알아보기 위한 것이다. 누룩 발효는 $36^{\circ}C$에서 30일간 발효한 처리구(TN-A)와 $45^{\circ}C$에서 10일간 전발효 후, $36^{\circ}C$에서 20일간 후 발효시킨 처리구(TN-B)로 실험하였다. 누룩 발효시 온도에 따른 ${\alpha}$-amylase, glucoamylase, acidic protease 등 효소활성과 누룩의 대사산물을 측정하였다. 초기 효소활성은 온도에 상관없이 급격하게 증가하였고 발효 3일 후, TN-A 누룩의 효소활성은 일정하게 유지됐으나 TN-B 누룩은 발효기간이 끝나는 동안 점진적으로 이들 활성이 감소하였다. $^1H$-MNR을 이용한 대사체 분석결과, TN-A 누룩은 glucose, glycerol, fructose, mannitol, lactose가 초기에 급격히 증가한 후 감소하였으나, TN-B 누룩은 초기에 대사체들이 감소한 후, 발효기간 동안 일정하게 유지되었다. Glycine, proline, serine 유리아미노산은 TN-B보다 TN-A 누룩에서 보다 높게 나타났다. 밀누룩 발효시 약 $36^{\circ}C$의 일정한 온도는 전분 및 단백질분해 효소의 높은 활성을 유지하는데 적절하였다.

• The Plant Pathology Journal
• /
• 제31권3호
• /
• pp.226-244
• /
• 2015

Fusarium head blight is one of the most important and most common diseases of winter wheat. In order to better understanding this disease and to assess the correlations between different factors, 30 cultivars of this cereal were evaluated in a two-year period. Fusarium head blight resistance was evaluated and the concentration of trichothecene mycotoxins was analysed. Grain samples originated from plants inoculated with Fusarium culmorum and naturally infected with Fusarium species. The genetic distance between the tested cultivars was determined and data were analysed using multivariate data analysis methods. Genetic dissimilarity of wheat cultivars ranged between 0.06 and 0.78. They were grouped into three distinct groups after cluster analysis of genetic distance. Wheat cultivars differed in resistance to spike and kernel infection and in resistance to spread of Fusarium within a spike (type II). Only B trichothecenes (deoxynivalenol, 3-acetyldeoxynivalenol and nivalenol) produced by F. culmorum in grain samples from inoculated plots were present. In control samples trichothecenes of groups A (H-2 toxin, T-2 toxin, T-2 tetraol, T-2 triol, scirpentriol, diacetoxyscirpenol) and B were detected. On the basis of Fusarium head blight assessment and analysis of trichothecene concentration in the grain relationships between morphological characters, Fusarium head blight resistance and mycotoxins in grain of wheat cultivars were examined. The results were used to create of matrices of distance between cultivars - for trichothecene concentration in inoculated and naturally infected grain as well as for FHB resistance Correlations between genetic distance versus resistance/mycotoxin profiles were calculated using the Mantel test. A highly significant correlation between genetic distance and mycotoxin distance was found for the samples inoculated with Fusarium culmorum. Significant but weak relationships were found between genetic distance matrix and FHB resistance or trichothecene concentration in naturally infected grain matrices.

• 한국작물학회지
• /
• 제50권5호
• /
• pp.346-355
• /
• 2005

To investigate the application of biochemical markers' and small-sample methods using whole-wheat flours for screening in early generation in Korean wheat breeding system, 74 Korean wheats, including cultivars, local breeding lines and experimental lines, were analyzed. Seed storage protein and amylose contents of grains were evaluated. Biochemical makers, including granule bound starch synthase (GBSS), high molecular weigh glutenin subunits (HMW-GS) and friabilin were also evaluated by using one-dimensional sodium dodecyl sulfate-polyacryla-mide gel electrophoresis with a single kernel. The small­sample methods, including modified SDS-sedimentation test (MST), micro-alkaline water retention capacity (AWRC) and whole-wheat flour swelling volume (WSV) were also tested in this study. Protein content, MST and AWRC was $11.0 - 15.8\%$, 2.7 - 26.2 ml and $71.9 - 109.7\%$, respectively. Apparent and total amylose content and WSV was $20.6 - 25.0\%$, $26.1 - 32.4\%$ and 9.0 - 16.9 ml, respectively. There were highly significant correlations between MST and AWRC (r=0.592, P<0.001), but Korean wheats showed no significant difference in protein content, amylose content and small-sample methods. In the biochemical markers, Korean wheats contained all three GBSS encoded by Wx loci, except for Suwon 252. Korean wheats showed the high frequency ($58.1\%$) of 1Dx2.2 + 1Dy12 subunits of HMW-GS. Friabilin band was present in 46 lines ($62.2\%$) and absent in 28 lines ($37.8\%$). Friabilin-absence lines showed the higher MST (14.9 ml) and AWRC ($92.1\%$) value than friabilin-presence lines (8.5 ml and $82.4\%$, respectively).