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http://dx.doi.org/10.5352/JLS.2010.20.7.999

β-Glucosidase Recovery from a Solid-State Fermentation System by Aspergillus niger  

Chandra, M. Subhosh (Department of Microbiology, Sri Krishnadevaraya University)
Reddy, B. Rajasekhar (Department of Microbiology, Yogi Vemana University)
Choi, Yong-Lark (Department of Biotechnology, College of Natural Resources and Life Science, Dong-a University)
Publication Information
Journal of Life Science / v.20, no.7, 2010 , pp. 999-1004 More about this Journal
Abstract
Investigations were carried out on a $\beta$-glucosidase produced by Aspergillus niger under solid-state fermentation conditions as a model of enzyme recovery from fermented wheat bran. The leaching efficiency of distilled water to recover the enzyme from the fermented bran was higher than acetate buffer, citrate buffer, citrate-phosphate buffer and 5% methanol; thus, the conditions were further optimized with distilled water as the extracting agent. After fermented bran was washed three times with distilled water for 1.5 hr each under shaking conditions at 1:5 solid to solvent ratio, a maximum recovery of 0.025 U/g of wheat bran was obtained.
Keywords
$\beta$-Glucosidase; wheat bran; solid-state fermentation; Aspergillus niger; recovery;
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1 Singh, J. and A. P. Garg. 1995. Production of cellulases by Gliocladium virens Miller et al. on Eichhornia under solid state fermentation conditions. J. Ind. Bot. Soc. 74, 305-309.
2 Tolan, J. S. and B. Foody. 1999. Cellulase from submerged fermentation. Adv. Biochem. Eng. Biotechnol. 65, 41-67.
3 Muniswaran, P. K. A. and N. C. L. N. Charyulu. 1994. Solid substrate fermentation of coconut coir pitch for cellulase production. Enzyme Microb. Technol. 16, 436-440.   DOI
4 Narasimha, G., G. V. A. K. Babu, and B. Rajasekhar Reddy. 1999. Cellulolytic activity of fungal cultures isolated from soil contaminated with effluents of cotton ginning industry. J. Environ. Biol. 20, 235-239.
5 Palit, S. and R. Banerjee. 2001. Optimization of extraction parameters for recovery of α-amylase from the fermented bran of Bacillus circulans GRS313. Braz. Arch. Biol. Technol. 44, 107-111.
6 Panagiotou, G., D. Kekos, B. J. Macris, and P. Christakopoulos. 2003. Production of cellulolytic and xylanolytic enzymes by Fusarium oxysporum grown on corn stover in solid state fermentation. Ind. Crop. Prod. 18, 37-45.   DOI
7 Ramakrishna, S. V., T. Suseela, N. P. Ghildyal, S. A. Jaleel, P. Prema, B. K. Lonsane, and S. Y. Ahmed. 1982. Recovery of amyloglucosidase from moldy bran. Ind. J. Technol. 20, 476-480
8 Ramamurthy, V. and R. M. Kothari. 1993. Comparison of fungal protease production by submerged and surface cultivation. J. Biotechnol. 27, 349-354.   DOI
9 Ramesh, M. V. and B. K. Lonsane. 1988. Chem. Mikrobiol. Technol. Lebnsm. 11, 155.
10 Lonsane, B. K. and M. V. Ramesh. 1990. Production of bacterial thermostable ${\alpha}-amylase$ by solid state fermentation: a potential tool for achieving economy in enzyme production and starch hydrolysis. Advances Appl. Microbiol. 35, 1-56.   DOI
11 Mitchell, D. A., Z. Targonski, A. Rogalski, and A. Leonowicz. 1992. Substrates for processes, pp. 29-52, In Doelle, H. W., D. A. Mitchell, and C. E. Rolz (eds.), Solid Substrate Cultivation. Elsevier, London,
12 Lonsane, B. K., N. P. Ghild, S. Budeatman, and S. V. Ramakrishna. 1985. Engineering aspects of solid state fermentation. Enzyme Microb. Technol. 7, 258-265.   DOI
13 Lynd, L. R., P. J. Weimer, W. H. Van Zyl, and I. S. Pretorius. 2002. Microbial cellulose utilization: Fundamentals and biotechnology. Microbiol. Mole. Biol. Rev. 66, 506-577.   DOI
14 McCarthy, A. J. 1987. Lignocellulose-degrading actinomycetes. FEMS Microbiol. Rev. 46, 145-163.   DOI
15 Heck, J. X., P. F. Hertz, and M. A. Z. Ayub. 2002. Cellulase and xylanase production by isolated Amazon Bacillus strains using soybean industrial residue based solid state cultivation. Braz. J. Microbiol. 33, 213-218.   DOI   ScienceOn
16 Leda, R. C., A. M. Ricardo, and L. M. A. Tito. 2000. Production and extraction of pectinases obtained by solid state fermentation of agroindustrial residues with Aspergillus niger. Bioresource Technol. 71, 45-50.   DOI
17 Lonsane, B. K. and M. M. Krishnaiah. 1992. Product leaching and downstream processing, pp. 147, In Solid Substrate Cultivation, In Doelle, H. W., D. A. Mitchell, and C. E. Rolz, (eds.), Elsevier Science Publishers. London.
18 Lonsane, B. K. and N. P. Ghildyal. 1992. Exoenzymes, pp. 191-209, In Doelle, H. W., D. A. Mitchell, and C. E. Rolz (eds.), Solid Substrate Cultivation. Elsevier, London.
19 Fernandez Lahore, H. M., E. R. Fraile, and O. Cascone. 1998. A protease recovery from a solid-state fermentation system. J. Biotechnol. 62, 83-93.   DOI
20 Hahn-Hagerdal, B. 1986. Enzyme Microbiol. Technol. 8, 322.   DOI
21 Herr, D. 1979. Secretion of cellulases and β-glucosidase by Trichoderma viride TTCC 1433 in submerged cultures on different substrates. Biotechnol. Bioeng. 21,1361-1363.   DOI
22 Illanes, A., G. Aroca, L. Cabello, and F. Acevedo. 1992. World J. Microbiol. Biotechnol. 8, 488-493.   DOI
23 Jecu, L. 2000. Solid state fermentation of agricultural wastes for endoglucanase production. Ind. Crop. Prod. 11, 1-5.   DOI
24 Kim, J. H., M. Hosobuchi, M. Kishimoto, T. Seki, T. Yoshida, H. Taguchi, and D. D. Y. Ryu. 1985. Cellulase production by a solid state culture system. Biotechnol. Bioeng. 27, 1445-1450.   DOI
25 Kotchoni, O. S. and O. O. Shonukan. 2002. Regulatory mutations affecting the synthesis of cellulase in B. pumulis. World J. Microbiol. Biotechnol. 18, 487-491.   DOI
26 Chandra, M. S., B. R. Reddy, and Y. L. Choi. 2008. Production of cellulolytic enzymes by Aspergillus niger on solid and submerged state fermentation. J. Life Sci. 18, 1049-1052.   DOI
27 Krishna, C. 1999. Production of bacterial cellulases by solid state bioprocessing of banana wastes. Bioresource Technol. 69, 231-239.   DOI
28 Krishna, C. and M. Chandrasekharan. 1996. Banana waste as substrate for α-amylase production by Bacillus subtilis (CBTK 106) under solid state fermentation. Appl. Microbiol. Biotechnol. 46, 106-111.   DOI
29 Castilho, L. R., T. L. M. Alves, and A. M. Ricardo. 1999. A Recovery of pectolytic enzymes produced by solid state culture of Aspergillus niger. Process Biochem. 34, 181-186.   DOI   ScienceOn
30 Chandra, M. S., B. R. Reddy, and Y. L. Choi. 2008a. Optimization of extraction of filter paperase from the fermented bran of Aspergillus niger in solid state fermentation. J. Appl. Biol. Chem. 51, 155-159.   DOI   ScienceOn
31 Chandra, M. S., B. Viswanath, and B. R. Reddy. 2007. Cellulolytic enzymes on lignocellulosic substrates in solid state fermentation by Aspergillus niger. Indian J. Microbiol. 47, 323-328.   DOI
32 Chundakhadu, K. 1999. Production of bacterial cellulases by solid state bioprocessing of banana wastes. Bioresource Technol. 69, 231-239.   DOI
33 Cihangir, N. and E. Sarikaya. 2004. Investigation of lipase production by a new isolate of Aspergillus sp. World J. Microbiol. Biotechnol. 20, 193-197.   DOI
34 Caltron, G. J., C. S. Coobs, and J. P. Hmman. 1986. Manual of Industrial Microbiology and Biotechnology, American society of microbiology, Washington. USA, 436-445.
35 Considine, P. J., A. O’Rorke, T. J. Hackett, and M. P. Coughlan. 1988. Hydrolysis of beet pulp polysaccharides by extracts of solid state cultures of Penicillium capsulatum. Biotechnol. Bioeng. 31, 433-438.   DOI
36 Fadel, M. 2000. Production physiology of cellulases and ${\beta}-glucosidase$ enzymes of Aspergillus niger grown under solid state fermentation conditions. J. Biol. Sci. 1, 401-411.   DOI
37 Badhan, A. K., B. S. Chadha, J. Kaur, H. S. Saini, and M. K. Bhat. 2007. Production of multiple xylanolytic and cellulolytic enzymes by thermophilic fungus Myceliophthora sp. IMI 387099. Bioresource Technol. 98, 504-510.   DOI