• Title/Summary/Keyword: wavefront

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Super-resolution Microscopy with Adaptive Optics for Volumetric Imaging

  • Park, Sangjun;Min, Cheol Hong;Han, Seokyoung;Choi, Eunjin;Cho, Kyung-Ok;Jang, Hyun-Jong;Kim, Moonseok
    • Current Optics and Photonics
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    • v.6 no.6
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    • pp.550-564
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    • 2022
  • Optical microscopy is a useful tool for study in the biological sciences. With an optical microscope, we can observe the micro world of life such as tissues, cells, and proteins. A fluorescent dye or a fluorescent protein provides an opportunity to mark a specific target in the crowd of biological samples, so that an image of a specific target can be observed by an optical microscope. The optical microscope, however, is constrained in resolution due to diffraction limit. Super-resolution microscopy made a breakthrough with this diffraction limit. Using a super-resolution microscope, many biomolecules are observed beyond the diffraction limit in cells. In the case of volumetric imaging, the super-resolution techniques are only applied to a limited area due to long imaging time, multiple scattering of photons, and sample-induced aberration in deep tissue. In this article, we review recent advances in super-resolution microscopy for volumetric imaging. The super-resolution techniques have been integrated with various modalities, such as a line-scan confocal microscope, a spinning disk confocal microscope, a light sheet microscope, and point spread function engineering. Super-resolution microscopy combined with adaptive optics by compensating for wave distortions is a promising method for deep tissue imaging and biomedical applications.

Development Plan for the First GMT ASM Reference Body

  • Yang, Ho-Soon;Oh, Chang-Jin;Biasi, Roberto;Gallieni, Daniele
    • The Bulletin of The Korean Astronomical Society
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    • v.46 no.2
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    • pp.76.3-77
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    • 2021
  • GMT secondary mirror system consists of 7 segmented adaptive mirrors. Each segment consists of a thin shell mirror, actuators and a reference body. The thin shell has a few millimeters of thickness so that it can be easily bent by push and pull force of actuators to compensate the wavefront disturbance of light due to air turbulence. The one end of actuator is supported by the reference body and the other end is adapted to this thin shell. One of critical role of the reference body is to provide the reference surface for the thin shell actuators. Therefore, the reference body is one of key components to succeed in development of GMT ASM. Recently, Korea Research Institute of Standards and Science (KRISS) and University of Arizona (UA) has signed a contract that they will cooperate to develop the first set of off-axis reference body for GMT ASM. This project started August 2021 and will be finished in Dec. 2022. The reference body has total 675 holes to accommodate actuators and 144 pockets for lightweighting. The rear surface has a curved rib shape with radius of curvature of 4387 mm with offset of 128.32mm. Since this reference body is placed just above the thin shell so that the front surface shape needs to be close to that of thin shell. The front surface has a concave off-axis asphere, of which radius of curvature is 4165.99 mm and off-axis distance is about 1088 mm. The material is Zerodur CTE class 1 (CTE=0.05 ppm/oC) from SCHOTT. All the actuator holes and pockets are machined normal to the front surface. It is a very complex challenging optical elements that involves sophisticated machining process as well as accurate metrology. After finishing the fabrication of reference body in KRISS, it will be shipped to UA for final touches and finally sent to Adoptica in Italy, in early 2023. This paper presets the development plan for the GMT ASM Reference Body and relevant fabrication and metrology plans.

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Technological development issues on geometric phase lens and its application of optical modulation (기하위상 렌즈의 개발 이슈 및 이의 광파 변조 응용)

  • Lee, Tae-Hyun;Lee, Su-Won;Hong, Keehoon;Choi, Kihong;Kim, Hak-Rin
    • Proceedings of the Korean Society of Broadcast Engineers Conference
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    • 2020.07a
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    • pp.557-560
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    • 2020
  • 최근, 광소자에서 공간 변조되는 wavefront profile 특성을 광소자의 표면 단차 변화 없이 단일 두께 박막 상에서 자유로이 구현할 수 있는 기하위상 홀로그램 (geometric phase hologram) 기반의 optical component에 대한 관심이 증대되고 있다. 특히 이를 이용해 제작된 기하위상 렌즈 (geometric phase lens)는 dynamic phase의 공간적 차이에 의해 구현되던 기존 bulk optics 기반의 lens 대비 초박형으로 제작이 가능한 파장 선택적 flat optics 기술로써, 다초점 및 경량화를 요구하는 차세대 디스플레이 기술 (augmented reality 또는 AR, mixed reality 또는 MR) 및 광파변조 및 제어를 요구하는 홀로그래픽 카메라 분야에 대한 응용처로 많은 주목을 받고 있다. 이에 본 논문에서는 해당 기하 위상렌즈에 대한 원리 및 이에 따른 개발이슈 및 해결법에 대해 연구 하였으며, 이에 대한 응용처로 기하위상 렌즈의 편광에 따른 이중초점특성을 이용해, 기존 단일 초점 형성이 가능한 AR기기 대비, 다초점 형성이 가능한 switchable dual-depth 3D AR device를 compact한 모듈과 함께 구현하였다. 또한, 기하위상렌즈의 광파 변조 및 분리특성을 이용한 기하위상 렌즈기반의 자가간섭 홀로그래픽 시스템(GP-self-interference incoherent digital holographic, GP-SIDH)에 편광 이미지센서 적용과 함께 맞춤형 설계/제작된 기하 위상렌즈를 적용함으로써, 기존 GP-SIDH 시스템대비 안정적으로 실시간 복소 홀로그램 획득이 가능한 실시간 공간영상정보 획득용 GP-SIDH을 동영상 프레임으로 구현하였다.

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Corneal Asphericity and Optical Performance after Myopic Laser Refractive Surgery (굴절교정수술을 받은 근시안의 각막 비구면도와 광학적 특성 평가)

  • Kim, Jeong-Mee;Lee, A-Young;Lee, Koon-Ja
    • Journal of Korean Ophthalmic Optics Society
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    • v.18 no.2
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    • pp.179-186
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    • 2013
  • Purpose: To compare corneal asphericity, visual acuity (VA), and ocular and corneal higher-order aberrations (HOAs) between myopic refractive surgery and emmetropia groups. Methods: Twenty three subjects ($23.0{\pm}2.5$ years) who underwent myopic refractive surgery and twenty emmetropia ($21.0{\pm}206$ years) were enrolled. The subjects'criteria were best unaided monocular VA of 20/20 or better in both two groups. High and low contrast log MAR visual acuities were measured under photopic and mesopic conditions. Corneal and ocular HOAs were measured using Wavefront Analyzer (KR-1W, Topcon) for 4 mm and 6 mm pupils. Corneal asphericity was taken by topography in KR-1W. Results: There was no significant difference in VA between two groups under either photopic or mesopic conditions. In ocular aberrations, there were significant differences in total HOAs, fourthorder and spherical aberration (SA) for a 6 mm between two groups (p=0.045, p<0.001, and p<0.001, respectively). In corneal aberrations, there was a significant difference in SA for 4 mm (p=0.001) and 6 mm (p<0.001) pupils between two groups and there were statistically significant differences in total HOAs (p<0.001) and fourth-order aberrations (p<0.001) between two groups for a 6 mm pupil. There was a significant correlation in emmetropia between Q-value and SA in ocular aberrations for 4 mm and 6 mm pupils (r=0.442, p=0.004, and r=0.519, p<0.001) and in corneal aberrations for 4 mm and 6 mm pupils (r=0.358, p=0.023, and r=0.646, p<0.001). No significant correlations were found between Q-value and SA in refractive surgery group. Conclusions: VA in myopic refractive surgery is better than or similar to emmetropia. Nevertheless, the more increasing pupil size is, the more increasing aberrations are. Thus, it could have an influence on the quality of vision at night.

Change of Spherical Aberration with Aspheric Soft Contact Lens Wear (비구면 소프트콘택트렌즈 착용에 의한 눈의 구면수차 변화)

  • Kim, Jeong Mee;Mun, Mi-Young;Kim, Young Chul;Lee, Koon-Ja
    • Journal of Korean Ophthalmic Optics Society
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    • v.17 no.4
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    • pp.365-372
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    • 2012
  • Purpose: To investigate ocular higher order aberrations (HOA) and spherical aberration changes caused by an aspheric soft contact lens designed to reduce spherical aberration (SA) of the eye. Methods: Fifty subjects who have successfully experienced soft contact lenses were refitted with aspheric design (Soflens Daily Disposable: SDD, Bausch+Lomb) soft contact lens. Ocular higher order aberrations (HOA) and stand alone SA were measured and analyzed for a 4-mm pupil size using Wave-Scan Wavefront$^{TM}$ aberrometer (VISX, Santa Clara, CA, USA). High and low contrast log MAR visual acuity and contrast sensitivity function (CSF) were also measured under photopic and mesopic conditions (OPTEC 6500 Vision Tester$^{(R)}$). All measurements were conducted monocularly with an undilated pupil. Results: The RMS mean values for total HOA with SDD contact lenses were significantly lower than those at with unaided eyes (p<0.001) and a reduction for SA in the SDD was close to the baseline SA (zero ${\mu}m$) (p<0.001). For the SDD lens, there was a statistically significant correlation between the changes in the total HOA and the contact lens power (r=0.237, p=0.018) as well as between the changes in SA and the lens power (r=0.324, p=0.001). High contrast visual acuity (HCVA) and low contrast visual acuity (LCVA) with SDD lenses were $-0.063{\pm}0.062$ and $0.119{\pm}0.060$, respectively under photopic and $-0.003{\pm}0.063$ and $0.198{\pm}0.067$, respectively under mesopic condition. Contrast Sensitivity Function (CSF) with SDD lenses under both photopic and mesopic conditions was $3.095{\pm}0.068$ and $3.087{\pm}0.074$, respectively. Conclusions: The SDD contact lens designed to control SA reduced the total ocular HOA and SA of the eye, resulting in compensating for positive SA of the eyes. Thus, the optical benefits of the lens with SA control would be adopted for improving the quality of vision.

Changes in Spherical Aberration and Coma Aberration after Wearing Aspheric Soft Contact Lens in Young Myopes (젊은 성인 근시안에서 비구면 소프트 콘택트렌즈 착용 후 구면수차와 코마수차의 변화)

  • Lim, Dong-Kyu;Kwon, Hyeok;Lee, Koon-Ja
    • The Korean Journal of Vision Science
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    • v.20 no.4
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    • pp.469-482
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    • 2018
  • Purpose : We investigated the change of spherical and comma aberrations after wearing aspheric soft contact lens (ASCL) in young myopes. Methods : Fifty young myopes ($23.15{\pm}1.70years$, spherical equivalent: $-2.90{\pm}1.75D$) were recruited and refractive errors were corrected using ASCL (Biotrue, Bausch+Lomb, USA). High order aberrations were measured in the 4 mm pupil size using the wavefront analyze and pupil sizes were measured with a pupillometer at the modes of scotopic condition (light off) at 3.5 m in the 100 lx illuminance condition. Results : Spherical aberrations and coma aberration of the 20s myopes were $0.026{\pm}0.031{\mu}m$ and $0.078{\pm}0.039{\mu}m$ respectively, and $0.019{\pm}0.026{\mu}m$ and $0.082{\pm}0.038{\mu}m$ after ASCL wear that spherical aberration was decreased and coma aberration was increased. However, spherical aberration was decreased in the 68% of the subject have positive spherical aberration, and increased in the 11% of the subject have negative spherical aberration. Coma aberration was increased in the 53% of the subject, did not change in the 19% of the subjects, and decreased in the 28% of the subject. Spherical aberration was not different with the refractive errors in low and moderate myopies, however, coma aberrations was higher in the higher myopes. Conclusion : In a scotopic condition without accommodation stimuli, spherical aberration is decreased after wearing ASCL, however in the subject have negative spherical aberration spherical aberration could be increased, and which is thought to be the influence of contact lens design and pupil size.

Design and Performance Analysis of an Off-Axis Three-Mirror Telescope for Remote Sensing of Coastal Water (연안 원격탐사를 위한 비축 삼반사경 설계와 성능 분석)

  • Oh, Eunsong;Kang, Hyukmo;Hyun, Sangwon;Kim, Geon-Hee;Park, YoungJe;Choi, Jong-Kuk;Kim, Sug-Whan
    • Korean Journal of Optics and Photonics
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    • v.26 no.3
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    • pp.155-161
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    • 2015
  • We report the design and performance analysis of an off-axis three-mirror telescope as the fore optics for a new hyperspectral sensor aboard a small unmanned aerial vehicle (UAV), for low-altitude coastal remote sensing. The sensor needs to have at least 4 cm of spatial resolution at an operating altitude of 500 m, $4^{\circ}$ field of view (FOV), and a signal to noise ratio (SNR) of 100 at 660 nm. For these performance requirements, the sensor's optical design has an entrance pupil diameter of 70 mm and an F-ratio of 5.0. The fore optics is a three-mirror system, including aspheric primary and secondary mirrors. The optical performance is expected to reach $1/15{\lambda}$ in RMS wavefront error and 0.75 in MTF value at 660 nm. Considering the manufacturing and assembling phase, we determined the alignment compensation due to the tertiary mirror from the sensitivity, and derived the tilt-tolerance range to be 0.17 mrad. The off-axis three-mirror telescope, which has better performance than the fore optics of other hyperspectral sensors and is fitted for a small UAV, will contribute to ocean remote-sensing research.

A Calculation of the Propagation for Focused Beams Using BPM (BPM을 이용한 안테나 배열의 집속 빔 전파 해석)

  • Kim Jaeheung;Cho Choon Sik;Lee Jae W.
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.16 no.5 s.96
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    • pp.465-471
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    • 2005
  • A method of calculation fur propagating and focusing of focused beams generated in antenna arrays, using BPM(Beam Propagation Method), is presented in this paper. Based on the diffraction theory, the beam focusing and Propagation is studied specially for the case of the antenna way fed by the Rotman lens that is able to focus microwave power on its focal arc or generate multiple beams. There are difficulties in performing a full-wave simulation using a commercial EM simulation tool for propagating and focusing of beams because of the structural complexity and the feeding assignment of the antenna array. Therefore, as an alternative solution, the BPM is presented to calculate the beam propagation from the aperture-type antennas. From the point of view of optics, the propagations of the lens have been simplified from the Fresnel diffraction integral to the Fourier transform. Using Fourier Transform, a beam propagation method is developed to show improvement of the resolution by controlling the wavefront of wave Propagating from an aperture-type antenna array. The beam width(or spot size) and the intensity are calculated for a focused beam propagating from an array having $10\lambda$ of its size. For the beams with $20\lambda,\;30\lambda$, and $50\lambda$ of geometrical focal length, the half-power beam widths(or spot size) are about 1.1\lambda,\;1.3\lambda,\;and\;1.9\lambda$ respectively.

Reverse-time migration using the Poynting vector (포인팅 벡터를 이용한 역시간 구조보정)

  • Yoon, Kwang-Jin;Marfurt, Kurt J.
    • Geophysics and Geophysical Exploration
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    • v.9 no.1
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    • pp.102-107
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    • 2006
  • Recently, rapid developments in computer hardware have enabled reverse-time migration to be applied to various production imaging problems. As a wave-equation technique using the two-way wave equation, reverse-time migration can handle not only multi-path arrivals but also steep dips and overturned reflections. However, reverse-time migration causes unwanted artefacts, which arise from the two-way characteristics of the hyperbolic wave equation. Zero-lag cross correlation with diving waves, head waves and back-scattered waves result in spurious artefacts. These strong artefacts have the common feature that the correlating forward and backward wavefields propagate in almost the opposite direction to each other at each correlation point. This is because the ray paths of the forward and backward wavefields are almost identical. In this paper, we present several tactics to avoid artefacts in shot-domain reverse-time migration. Simple muting of a shot gather before migration, or wavefront migration which performs correlation only within a time window following first arriving travel times, are useful in suppressing artefacts. Calculating the wave propagation direction from the Poynting vector gives rise to a new imaging condition, which can eliminate strong artefacts and can produce common image gathers in the reflection angle domain.

Aberration Retrieval Algorithm of Optical Pickups Using the Extended Nijboer-Zernike Approach (확장된 네이보어-제르니케 방법에 의한 광픽업의 파면수차 복원 알고리즘)

  • Jun, Jae-Chul;Chung, Ki-Soo;Lee, Gun-Kee
    • Journal of the Institute of Convergence Signal Processing
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    • v.11 no.1
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    • pp.32-40
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    • 2010
  • In this work, the method of acquiring the pupil function of optical system is proposed. The wavefront aberration and the intensity distribution of pupil can be analysed with the pupil function. This system can be adopted to the manufacturing line of optical pickup directly and also has good performance to analysing various property of optical instrument. It is one kind of inverse problem to get pupil functions by 3D beam data. The extended Nijboer-Zernike(ENZ) approach recently proposed by Netherlands research group is adopted to accompany to solve these inverse problem. The ENZ approach is one of a aberration retrieval method for which numerous approaches are available. But this approach is new in the sense that it use the highly efficient representation of pupil functions by means of their Zernike coefficients. These coefficients are estimated by using matching procedure in the focal region the theoretical 3D intensity distribution and measured 3D intensity distribution. The algorithm that can be applied more general circumstance such as high-numerical aperture instrument is developed by modifying original ENZ approach. By these scheme, MS windows based GUI program is developed and the good performance is verified with generated 3D beam data.