• Molecules and Cells
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• v.44 no.9
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• pp.627-636
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• 2021

The three-dimensional organization of chromatin and its time-dependent changes greatly affect virtually every cellular function, especially DNA replication, genome maintenance, transcription regulation, and cell differentiation. Sequencing-based techniques such as ChIP-seq, ATAC-seq, and Hi-C provide abundant information on how genomic elements are coupled with regulatory proteins and functionally organized into hierarchical domains through their interactions. However, visualizing the time-dependent changes of such organization in individual cells remains challenging. Recent developments of CRISPR systems for site-specific fluorescent labeling of genomic loci have provided promising strategies for visualizing chromatin dynamics in live cells. However, there are several limiting factors, including background signals, off-target binding of CRISPR, and rapid photobleaching of the fluorophores, requiring a large number of target-bound CRISPR complexes to reliably distinguish the target-specific foci from the background. Various modifications have been engineered into the CRISPR system to enhance the signal-to-background ratio and signal longevity to detect target foci more reliably and efficiently, and to reduce the required target size. In this review, we comprehensively compare the performances of recently developed CRISPR designs for improved visualization of genomic loci in terms of the reliability of target detection, the ability to detect small repeat loci, and the allowed time of live tracking. Longer observation of genomic loci allows the detailed identification of the dynamic characteristics of chromatin. The diffusion properties of chromatin found in recent studies are reviewed, which provide suggestions for the underlying biological processes.

• Journal of the Korea Convergence Society
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• v.10 no.2
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• pp.149-158
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• 2019

Cyberbullying, one of the acts of cyber violence, is becoming a problem as digital devices become popular. Therefore, this study intends to provide pictograms that can communicate visually in order to raise awareness of cyberbullying prevention. 'Cyberbullying prevention pictogram' was conducted in total 6 times for 5 middle and high school boys. After analyzing the works, the results of this study are as follows. First, we investigated the change of cyberbullying recognition, empathy sensitivity, message visualization and social participation. First, the learner relaxed the burden of the art expression by using app, and the interest of the art was improved. Second, the learner recognized the seriousness of cyberbullying's violence and recognized the importance of preventing cyberbullying. Third, the learner improved sympathy sensitivity that sympathizes the suffering of cyberbullying victims. Fourth, cyberbullying prevention pictograms improved the ability to read, utilize and interpret visual images through sharing works. Fifth, the work was posted on an Instagram to give concrete action on social problems. In conclusion, we proved the effectiveness of cyberbullying prevention art class using pictograms.

• Smart Media Journal
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• v.8 no.1
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• pp.67-73
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• 2019

This study investigates Active Learning and Facilitation Convergence Education Program which can improve core competency to cope with vocational education in the fourth industrial revolution era. I applied the integrated advantages of Active Learning which enhances 'problem solving skill' and those of Facilitation for creative thinking idea to application design process coursework and verified the effectiveness of such education method through student satisfaction survey. I also designed application contents for the students who are familiar with the mobile environments and UI contents for data visualization which can help those students to improve their skills in software. Every coursework was conducted as a team project. As a result, Active Learning and Facilitation Convergence Education Program is found to be helpful in improving the basic skills and competencies required in college education. I hope this work helps to reduce the educational gap between industry and professional colleges.

• The Journal of the Convergence on Culture Technology
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• v.7 no.3
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• pp.571-576
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• 2021

This study was carried out to overcome the problems of the existing fitness business and to build a fitness system that can meet the increased demand in the Corona situation. As a platform technology for non-face-to-face fitness edutainment service, it is a next-generation fitness exercise device that can use various body parts and synchronize network-type information. By synchronizing the exercise information of the fitness equipment, it was composed of learning contents through MR-based avatars. A quantified result was derived from examining the applicability of the customized evaluation system through momentum analysis with A.I analysis applying the LSTM-based algorithm according to the cumulative exercise effect of the user. It is a motion capture and 3D visualization fitness program for the application of systematic exercise techniques through academic experts, and it is judged that it will contribute to the improvement of the user's fitness knowledge and exercise ability.

• International conference on construction engineering and project management
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• 2022.06a
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• pp.408-417
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• 2022

Although traditionally perceived as being a visualization and asset management resource, the relatively rapid rate of improvement of computing power, coupled with the proliferation of cloud and edge computing and the IoT has seen the expanded functionality of modern Digital Twins (DTs). These technologies, when applied to buildings, are now providing users with the ability to analyse and predict their energy consumption, implement building controls and identify faults quickly and efficiently, while preserving acceptable comfort and well-being levels. Furthermore, when these building DTs are linked together to form a community DT, entirely new and novel energy management techniques, such as demand side management, demand response, flexibility and local energy markets can be unlocked and analysed in detail, creating circularity in the economy and making ordinary building occupants active participants in the energy market. Through the EU Horizon 2020 funded TwinERGY project, three different levels of DT (consumer - building - community) are being created to support the creation of local energy markets while optimising building performance for real-time occupant preferences and requirements for their building and community. The aim of this research work is to demonstrate the development of this new, interrelated, multi-level DT that can be used as a decision-making tool, helping to determine optimal scenarios simultaneously at consumer, building and community level, while enhancing and successfully supporting the community's management plan implementation.

• Investigative Magnetic Resonance Imaging
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• v.16 no.1
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• pp.47-54
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• 2012

Purpose : This study was performed to evaluate the characteristics of rat mesenchymal stem cells (RMSCs) transduced with human ferritin gene and investigate $in$ $vitro$ MRI detectability of ferritin-transduced RMSCs. Materials and Methods: The RMSCs expressing both myc-tagged human ferritin heavy chain subunit (myc-FTH) and green fluorescence protein (GFP) were transduced with lentiviurs. Transduced cells were sorted by GFP expression using a fluorescence-activated cell sorter. Myc-FTH and GFP expression in transduced cells were detected by immunofluorescence staining. The cell proliferative ability and viability were assessed by MTT assay. The RMSC surface markers (CD29+/CD45-) were analyzed by flow cytometry. The intracellular iron amount was measured spectrophotometically and the presence of ferritin-iron accumulation was detected by Prussian blue staining. $In$ $vitro$ magnetic resonance imaging (MRI) study of cell phantoms was done on 9.4 T MR scanner to evaluate the feasibility of imaging the ferritin-transduced RMSCs. Results: The myc-FTH and GFP genes were stably transduced into RMSCs. No significant differences were observed in terms of biologic properties in transduced RMSCs compared with non-transduced RMSCs. Ferritin-transduced RMSCs exhibited increased iron accumulation ability and showed significantly lower $T_2$ relaxation time than non-transduced RMSCs. Conclusion: Ferritin gene as MR reporter gene could be used for non-invasive tracking and visualization of therapeutic mesenchymal stem cells by MRI.

• Journal of Gifted/Talented Education
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• v.15 no.1
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• pp.67-84
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• 2005

In this study we investigated the adequacy of tools for distinction gifted students through the comparison these mutual relation on the basis of data, like paper test, the depths interview score, and the rest data((TTCT: Torrance Tests of Creative Thinking, IQ test, FASP: Find A Shape Puzzle, V.T: Visualization Tests and Exp: experimental ability test), and analysis data of EEG test for examining the adequacy of tools for identification gifted students. So, we developed Brain Wave gifted Index(G-Index) for finding another distinction ability as using brain waves data. The standard of index development use gifted brain characteristic in closed-eyes rest state which is judged like that characteristic of distinction between gifted and normal students is the most clear and consistence. That is, the degree of unified pattern between each object and gifted PCA pattern was defined by Pearson method which added spatial mutual index to weight concept. This refer to mean number of spatial PCA pattern. Searching for the possibility of distinction gifted gave distinction effect in 76%. The result of regression analysis on the basis of mutual relation between the rest data is . The probability formula for distinct gifted group is as follow. $$P=\frac 1{1+e^{-[-0.018(TTCT)+0.057(IQ)+1.916(FASP)+0.682(V.T)+0.088(Exp.)+0.034(G-Index)-57.510]}}$$ The result of this calculation showed that probability for distinct in gifted group was very good(95.0%). On the basis of upper result, tools for identification gifted students should be estimated as using many-sided estimation data whatever possible. And following study about development, and operation of tools for distinction suitable to gifted student in science should be progressed.

• The Journal of the Convergence on Culture Technology
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• v.9 no.6
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• pp.1173-1178
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• 2023

In modern society, there is a tendency to emphasize efficiency and lead to detailed planning of team projects and individual tasks within organizations. In a 24-hour routine, the ability to use time effectively is considered an indicator of conscientiousness, and people try to imitate planned and organized people and utilize various systems to manage their daily lives. The reason why you want to perform a given task efficiently is because it affects the success or failure of the project by completing the task within a set period of time. Neglecting the project schedule is considered a major risk that threatens a successful outcome. This applies not only to large-scale organizational projects but also to individual life, and utilizes a variety of schedule management tools that emphasize time-based efficient management. In project management, it is necessary to carefully understand the detailed work progress rather than simply based on Today. In this paper, we propose an Android application that can manage schedules by accessing the user's project in units of tasks rather than dates, and introduce its implementation. The application we implemented in this paper can manage the project's goals and schedule by registering the project to which the user belongs. In addition, it provides the ability to approach work efficiently by visualizing the progress of the entire project or individual goals. As a result, users can use the application we propose in this paper to focus on their projects and manage schedules by task, thereby improving the overall efficiency of the project.

• Journal of Industrial Convergence
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• v.22 no.1
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• pp.105-115
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• 2024

This study aimed to analyze the impact of mental strength on the competitive performance of student Taekwondo sparring athletes. A total of 343 middle school, high school, and university students registered as Taekwondo sparring athletes with the Korea Taekwondo Association were conveniently sampled. The Mental Toughness Test developed by Loehr was utilized after expert consultations. Data analysis involved t-tests and one-way ANOVA to assess the levels of mental strength sub-factors based on general characteristics, followed by post hoc tests using the Schéffe method for intergroup comparisons. Correlation analysis and multiple regression were conducted to examine the relationship between sub-factors of mental strength and competitive ability. The results indicated significant differences in mental strength sub-factors-confidence, level of awakening regulation, visualization and mental imagery regulation, motivation level, positive energy, and attitude control-based on gender and age among Taekwondo sparring student athletes. In terms of perceived competitive ability, significant differences were found based on age and sports experience. Consequently, beyond psychological training, the study revealed that age and diverse experiences positively influence specific aspects of mental strength among Taekwondo sparring student athletes. Therefore, coaching and training for these athletes, particularly during middle and high school years, should incorporate psychological aspects alongside diverse competition experiences and training to help overcome performance evaluation anxieties during matches.

• Journal of Practical Agriculture & Fisheries Research
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• v.20 no.2
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• pp.57-72
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• 2018

In order to extract meaningful information from the excellent farming settlement cases of young farmers published by KNCAF, we studied the key words with text mining and created a word cloud for visualization. First, in the text mining results for the entire sample, the words 'CEO', 'corporate executive', 'think', 'self', 'start', 'mind', and 'effort' are the words with high frequency among the top 50 core words. Their ability to think, judge and push ahead with themselves is a result of showing that they have ability of to be managers or managers. And it is a expression of how they manages to achieve their dream without giving up their dream. The high frequency of words such as "father" and "parent" is due to the high ratio of parents' cooperation and succession. Also 'KNCAF', 'university', 'graduation' and 'study' are the results of their high educational awareness, and 'organic farming' and 'eco-friendly' are the result of the interest in eco-friendly agriculture. In addition, words related to the 6th industry such as 'sales' and 'experience' represent their efforts to revitalize farming and fishing villages. Meanwhile, 'internet', 'blog', 'online', 'SNS', 'ICT', 'composite' and 'smart' were not included in the top 50. However, the fact that these words were extracted without omission shows that young farmers are increasingly interested in the scientificization and high-tech of agriculture and fisheries Next, as a result of grouping the top 50 key words by crop, the words 'facilities' in livestock, vegetables and aquatic crops, the words 'equipment' and 'machine' in food crops were extracted as main words. 'Eco-friendly' and 'organic' appeared in vegetable crops and food crops, and 'organic' appeared in fruit crops. The 'worm' of eco-friendly farming method appeared in the food crops, and the 'certification', which means excellent agricultural and marine products, appeared only in the fishery crops. 'Production', which is related to '6th industry', appeared in all crops, 'processing' and 'distribution' appeared in the fruit crops, and 'experience' appeared in the vegetable crops, food crops and fruit crops. To visualize the extracted words by text mining, we created a word cloud with the entire samples and each crop sample. As a result, we were able to judge the meaning of excellent practices, which are unstructured text, by character size.