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Semi-selective Medium for Monitoring Colletotrichum acutatum Causing Pepper Anthracnose in the Field (고추 탄저병균Colletotrichum acutatum 의 포장 밀도 조사를 위한 반선택 배지의 확립 및 활용)

  • Kang, Beum-Kwan;Min, Ji-Young;Kim, Yun-Sik;Park, Sung-Woo;Nguyen, Van-Bach;Kim, Heung-Tae
    • Research in Plant Disease
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    • v.11 no.1
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    • pp.21-27
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    • 2005
  • It was confirmed that anthracnose pathogen, Colletotrichum acutatum, could specifically grow on PDA amended with $100\mu\textrm{g}$ /ml of ampicillin and tetracycline, and 100 $100\mu\textrm{g}$/ml of mixture with carbendazim and diethofencarb. There was a positive correlation between the number of colony enumerated on semi-selective media and the disease severity on pepper fruits caused by C. acutatum. Using semi-selective media for C. acutatum, the number of pathogen on soil and plant debris infected by anthracnose pathogen was investigated. In plant debris, the colony number of C. acutatum was more than in soil. For the identification of colony appeared on semi-selective media, 10 isolates were selected randomly. They were identified as C. acutatum through PCR using C. acutatum-specific primer.

Measuring the Environmental Attitudes for National Park Visitors : Application of New Environmental Paradigm (국립공원(國立公園) 방문객(訪問客)의 자연환경태도(自然環境態度) 측정(測定) : New Environmental Paradigm의 적용(適用))

  • Han, Sang-Yoel
    • Journal of Korean Society of Forest Science
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    • v.89 no.5
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    • pp.598-608
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    • 2000
  • This study examined several characteristics such as the relationships between environmental attitudes and national park visitors' socio-economic characteristics, and between environmental attitudes and their travel motivations. Environmental attitude was measured using the modified New Environmental Paradigm(NEP) scale, where five response options existed(from strongly disagree to strongly agree). Data for this empirical analysis were obtained from 2,647 participants at six national parks-Sulaksan, Kayasan, Naeiangsan, Pookhansan, Taeanhaean, and Hanryohaesang. Results showed that most visitors gave a pro-NEP response ranging from neutral attitude to pro-environmentalism. Environmental attitudes correlated positively with education level, while, negatively with age. Environmental concern, also, generally correlated positively with 'rest' and 'health' motivators, while, negatively with 'socialization' and 'vacation' motivators. The findings of this study suggest that education is the most effective politic measure to lead pro-environmental concern for park visitors. And, given the nature of the relationship between environmental concern and visitors' motivation, park managers should be more careful handling with family and group visitors who have 'vacation' and 'socialization' motivators than 'rest' and 'health' motivators.

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Bhumipol Dam Operation Improvement via smart system for the Thor Tong Daeng Irrigation Project, Ping River Basin, Thailand

  • Koontanakulvong, Sucharit;Long, Tran Thanh;Van, Tuan Pham
    • Proceedings of the Korea Water Resources Association Conference
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    • 2019.05a
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    • pp.164-175
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    • 2019
  • The Tor Tong Daeng Irrigation Project with the irrigation area of 61,400 hectares is located in the Ping Basin of the Upper Central Plain of Thailand where farmers depended on both surface water and groundwater. In the drought year, water storage in the Bhumipol Dam is inadequate to allocate water for agriculture, and caused water deficit in many irrigation projects. Farmers need to find extra sources of water such as water from farm pond or groundwater as a supplement. The operation of Bhumipol Dam and irrigation demand estimation are vital for irrigation water allocation to help solve water shortage issue in the irrigation project. The study aims to determine the smart dam operation system to mitigate water shortage in this irrigation project via introduction of machine learning to improve dam operation and irrigation demand estimation via soil moisture estimation from satellite images. Via ANN technique application, the inflows to the dam are generated from the upstream rain gauge stations using past 10 years daily rainfall data. The input vectors for ANN model are identified base on regression and principal component analysis. The structure of ANN (length of training data, the type of activation functions, the number of hidden nodes and training methods) is determined from the statistics performance between measurements and ANN outputs. On the other hands, the irrigation demand will be estimated by using satellite images, LANDSAT. The Enhanced Vegetation Index (EVI) and Temperature Vegetation Dryness Index (TVDI) values are estimated from the plant growth stage and soil moisture. The values are calibrated and verified with the field plant growth stages and soil moisture data in the year 2017-2018. The irrigation demand in the irrigation project is then estimated from the plant growth stage and soil moisture in the area. With the estimated dam inflow and irrigation demand, the dam operation will manage the water release in the better manner compared with the past operational data. The results show how smart system concept was applied and improve dam operation by using inflow estimation from ANN technique combining with irrigation demand estimation from satellite images when compared with the past operation data which is an initial step to develop the smart dam operation system in Thailand.

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Evaluation of Antioxidant, Tyrosinase and Collagenase Inhibitory of Grateloupia elliptica Extracts after Aureobasidium pullulans Fermentation (흑효모를 이용한 참도박 발효 추출물의 항산화 효과와 티로시나제 및 콜라게나제 저해효과)

  • Vu, Van Vinh;Lee, Kyung Eun;Kang, Sang Gu
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.46 no.1
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    • pp.1-9
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    • 2020
  • In this experiment, the Grateloupia elliptica (G. elliptica) was fermented using the fungus Aureobasidium pullulans (A. pullulans) and its extract was obtained from hot water and 70% ethanol solution. The extract was studied for their biological activities such as antioxidant effect, Collagenase and tyrosinase inhibition in comparison to the nonfermented exatract in same solvents. Antioxidative activity test using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) showed that ethanol extract had higher antioxidant activity than water extract. Among all of the samples, the antioxidant activity of G. elliptica fermented ethanol extracts (GEFEE) was highest. Tyrosinase inhibitory activity of GEFEE was highest with 9.8% at the 1,000 ㎍/mL concentration. No inhibition of collagenase from G. elliptica water extract (GEWE) and G. elliptica fermented water extract (GEFWE) was observed, but G. elliptica ethanol extracts (GEEE) and GEFEE showed increased collagenase inhibition activity with increasing concentrations of them. Collagenase inhibitory activity of GEFEE was highest with 50.3% at the 1,000 ㎍/mL concentration. MTS cell proliferation assay was conducted with the GEWE, GEEE, GEFWE, GEFEE and cell viability was over 90% at the 10 ㎍/mL ~ 1000 ㎍/mL concentrations for all of the samples, which suggested that the extracts were noncytotoxic. In conclusion, fermented extracts of G. elliptica could be developed to bioactive functional material for cosmetics with antioxidant and wrinkle improvement effects.

Brillouin Light Scattering Study of Magnetic Anisotropy in GaAs/Fe/Au System (Brillouin Light Scattering을 이용한 GaAs/Fe/Au 구조의 자기이방성)

  • Ha, Seung-Seok;You, Chun-Yeol;Lee, Suk-Mock;Ohta, Kenta;Nozaki, Takayuk;Suzuki, Yoshishige;Roy, W. Van
    • Journal of the Korean Magnetics Society
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    • v.18 no.4
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    • pp.147-153
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    • 2008
  • It has been well-known that the Fe/GaAs heterostructure has a small lattice mismatch of 1.4% between Fe and GaAs, and the Fe layer is grown epitaxially on the the GaAs substrate. There are rich physics are observed in the GaAs/Fe interface, and the spininjection is actively studied due to its potential applications for spintronics devices. We fabricated Fe wedge layer in the thickness range $0{\sim}3.4$ nm on the GaAs(100) surface with 5-nm thick Au capping layer. The magnetic anisotropy of the Fe/GaAs system was investigated by employing Brillouin light scattering(BLS) measurements in this study. The spin wave excitation of Fe layer was studied as the function of intensity and the in-plane angle of external magnetic field, and thickness of Fe layer. Also these various dependences were analyzed with analytic expression of spin wave surface mode in order to determine the magnetic anisotropies. It has been found that the GaAs/Fe/Au system has additional uniaxial magnetic anisotropy, while the bulk Fe has biaxial anisotropy. The uniaxial anisotropy shows increasing dependency respected to decreasing thickness of Fe layer while biaxial anisotropy is reduced with Fe film thickness. This result allows the analysis that the uniaxial anisotropy is originated from interface between GaAs surface and Fe layer.

Preparation and Characterization of PLGA Scaffold Impregnated Keratin for Tissue Engineering Application (케라틴이 함유된 조직공학적 PLGA 지지체의 제조 및 특성 분석)

  • Oh, A-Young;Kim, Soon-Hee;Lee, Sang-Jin;Yoo, James J.;Dyke, Mark van;Rhee, John M.;Khang, Gil-Son
    • Polymer(Korea)
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    • v.32 no.5
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    • pp.403-408
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    • 2008
  • Keratin is the major structural fibrous protein providing outer covering such as wool, hair, and nail. Keratin is useful as natural protein. We developed the keratin loaded poly(L-lactide-co-glycolide) (PLGA) scaffolds (keratin/PLGA) for the possibility of the application of the tissue engineering using bone marrow mesenchymal (BMSCs). Keratin/PLGA (contents 0%, 10%, 20% and 50% of PLGA weight) scaffolds were prepared by solvent casting/salt leaching method. We characterized porosity, wettability, and water uptake ability, DSC of keratin/PLGA scaffold. We seeded BMSCs isolated from the femurs of rat into the inner core of the hybrid scaffold. Celluar viability were assayed by 3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyl-tetrazolium bromide (MTT) test. We confirmed that keratin/PLGA scaffold is hydrophilic by wettability, and water uptake ability measurement results. In MTT assay results, cell viability in scaffolds impregnated 10 and 20 wt% of keratin were higher than other scaffolds. In conclusion, we suggest that keratin/PLGA scaffold may be useful to tissue engineering using BMSCs.

Meat quality characteristics of pork bellies in relation to fat level

  • Hoa, Van-Ba;Seol, Kuk-Hwan;Seo, Hyun-Woo;Seong, Pil-Nam;Kang, Sun-Moon;Kim, Yun-Seok;Moon, Sung-Sil;Kim, Jin-Hyoung;Cho, Soo-Hyun
    • Animal Bioscience
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    • v.34 no.10
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    • pp.1663-1673
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    • 2021
  • Objective: Pork belly is considered as the most commercially important and preferable primal cut by consumers worldwide. Thus, this study was conducted to determine the effects of fat levels on the meat quality characteristics of pork bellies. Methods: Seventy-eight growing-finishing pigs collected from different commercial pig farms were slaughtered and used in the present study. After slaughter 24 h, bellies were fabricated according to the Korean Pork Cutting Specification, and immediately sampled for analysis of their fat content. Based on the fat levels, the bellies were segregated into three different groups: low fat (LF, fat ≤20%, n = 15), medium fat (MF, fat 21% to 30%, n = 30), and high fat (HF, fat ≥31%, n = 33). The bellies were then analyzed for meat quality traits, fatty acids, flavor compounds and eating quality properties. Results: The HF group had lower moisture and cooking loss levels compared to the other groups (p<0.05). The LF group presented higher proportions of polyunsaturated fatty acids compared to the other groups (p<0.05). The LF group showed higher amounts of the Maillard reaction-derived flavor compounds (e.g., 2,5-dimethyl pyrazine, 2-ethyl-3,5-dimethyl, and 4-methylthiazole) associated with meaty and roasty flavors whereas, the HF group showed higher amounts of oleic acid- derived compounds (e.g., nonanal and octanal) associated with the fatty and oily flavors. Interestingly, significantly higher scores for all the eating quality attributes (flavor, juiciness, tenderness, and overall acceptance) were found in the HF group compared to those in the LF or MF group (p<0.05). Conclusion: The high-fat bellies (fat ≥31%) had a better technological quality and eating quality compared to the low-fat bellies (fat ≤20%). Thus, increasing the fat content may improve the technological quality and eating quality traits of pork bellies, however, this increase may also result in more trimmed loss due to excessively deposited body fat.

Techno-Economic Analysis of Reversible Solid Oxide Fuel Cell System Couple with Waste Steam (폐스팀을 이용한 가역 고체산화물 연료전지의 기술적 경제적 해석)

  • GIAP, VAN-TIEN;LEE, YOUNG DUK;KIM, YOUNG SANG;AHN, KOOK YOUNG
    • Transactions of the Korean hydrogen and new energy society
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    • v.30 no.1
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    • pp.21-28
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    • 2019
  • Reversible solid oxide fuel cell (ReSOC) system was integrated with waste steam for electrical energy storage in distributed energy storage application. Waste steam was utilized as external heat in SOEC mode for higher hydrogen production efficiency. Three system configurations were analyzed to evaluate techno-economic performance. The first system is a simple configuration to minimize the cost of balance of plant. The second system is the more complicated configuration with heat recovery steam generator (HRSG). The third system is featured with HRSG and fuel recirculation by blower. Lumped models were used for system performance analyses. The ReSOC stack was characterized by applying area specific resistance value at fixed operating pressure and temperature. In economical assessment, the levelized costs of energy storage (LCOS) were calculated for three system configurations based on capital investment. The system lifetime was assumed 20 years with ReSOC stack replaced every 5 years, inflation rate of 2%, and capacity factor of 80%. The results showed that the exergy round-trip efficiency of system 1, 2, 3 were 47.9%, 48.8%, and 52.8% respectively. The high round-trip efficiency of third system compared to others is attributed to the remarkable reduction in steam requirement and hydrogen compression power owning to fuel recirculation. The result from economic calculation showed that the LCOS values of system 1, 2, 3 were 3.46 ¢/kWh, 3.43 ¢/kWh, and 3.14 ¢/kWh, respectively. Even though the systems 2 and 3 have expensive HRSG, they showed higher round-trip efficiencies and significant reduction in boiler and hydrogen compressor cost.

Metabolizable energy requirement for maintenance estimated by regression analysis of body weight gain or metabolizable energy intake in growing pigs

  • Liu, Hu;Chen, Yifan;Li, Zhongchao;Li, Yakui;Lai, Changhua;Piao, Xiangshu;van Milgen, Jaap;Wang, Fenglai
    • Asian-Australasian Journal of Animal Sciences
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    • v.32 no.9
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    • pp.1397-1406
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    • 2019
  • Objective: Feed energy required for pigs is first prioritized to meet maintenance costs. Additional energy intake in excess of the energy requirement for maintenance is retained as protein and fat in the body, leading to weight gain. The objective of this study was to estimate the metabolizable energy requirements for maintenance ($ME_m$) by regressing body weight (BW) gain against metabolizable energy intake (MEI) in growing pigs. Methods: Thirty-six growing pigs ($26.3{\pm}1.7kg$) were allotted to 1 of 6 treatments with 6 replicates per treatment in a randomized complete block design. Treatments were 6 feeding levels which were calculated as 50%, 60%, 70%, 80%, 90%, or 100% of the estimated ad libitum MEI ($2,400kJ/kg\;BW^{0.60}\;d$). All pigs were individually housed in metabolism crates for 30 d and weighed every 5 d. Moreover, each pig from each treatment was placed in the open-circuit respiration chambers to measure heat production (HP) and energy retained as protein ($RE_p$) and fat ($RE_f$) every 5 d. Serum biochemical parameters of pigs were analyzed at the end of the experiment. Results: The average daily gain (ADG) and HP as well as the $RE_p$ and $RE_f$ linearly increased with increasing feed intake (p<0.010). ${\beta}$-hydroxybutyrate concentration of serum tended to increase with increasing feed intake (p = 0.080). The regression equations of MEI on ADG were MEI, $kJ/kg\;BW^{0.60}\;d=1.88{\times}ADG$, g/d+782 ($R^2=0.86$) and $ME_m$ was estimated at $782kJ/kg\;BW^{0.60}\;d$. Protein retention of growing pigs would be positive while REf would be negative at this feeding level via regression equations of $RE_p$ and $RE_f$ on MEI. Conclusion: The $ME_m$ was estimated at $782kJ/kg\;BW^{0.60}\;d$ in current experiment. Furthermore, growing pigs will deposit protein and oxidize fat if provided feed at the estimated maintenance level.

How are Good Dogs Made? : Focusing on the Cultural Intermediary Work of Dog Trainers in EBS1 and KBS2 (좋은 반려견은 어떻게 만들어지는가? : EBS1 <세상에 나쁜 개는 없다>와 KBS2 <개는 훌륭하다>의 반려견 훈련사의 문화매개 작업을 중심으로)

  • Choo, Hye-Won
    • Journal of Korea Entertainment Industry Association
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    • v.14 no.4
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    • pp.17-29
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    • 2020
  • This research looked into the two programs There are No Bad Dogs in the World(EBS1) and The Dogs are Respectable(KBS2) that can be considered as one of the factors that changed the culture of dog rearing in the country. Focus was also directed toward the cultural intermediary work of trainer Hyung-Wook Kang and veterinarian Chae-Hyun Seol, and the linguistic texts and visual images that describe the programs were analyzed. The findings showed that the two experts reproduce dog-related beliefs and values using devices such as "serious" storytelling, dog bodies, and guardian re-education. They perform cultural intermediary work in that they create the value of the companion dog culture in these programs and provide corresponding products and services to consumers through direct face-to-face interactions. This study contributes academically in the following respects. First, the issue of companion dogs in Korea was approached from a cultural point of view rather than bounding analysis to the industrial and economic level called the "petconomy" (pet + economy). Second, the concept of cultural intermediaries and related theories were examined, along with how the two experts engender the value of dog training in the broadcasting program. The results are expected to help illustrate the diversity of Korean dog culture.