• Title/Summary/Keyword: vacuum time

Search Result 1,627, Processing Time 0.026 seconds

Overexpression of ginseng patatin-related phospholipase pPLAIIIβ alters the polarity of cell growth and decreases lignin content in Arabidopsis

  • Jang, Jin Hoon;Lee, Ok Ran
    • Journal of Ginseng Research
    • /
    • v.44 no.2
    • /
    • pp.321-331
    • /
    • 2020
  • Background: The patatin-related phospholipase AIII family (pPLAIIIs) genes alter cell elongation and cell wall composition in Arabidopsis and rice plant, suggesting diverse commercial purposes of the economically important medicinal ginseng plant. Herein, we show the functional characterization of a ginseng pPLAIII gene for the first time and discuss its potential applications. Methods: pPLAIIIs were identified from ginseng expressed sequence tag clones and further confirmed by search against ginseng database and polymerase chain reaction. A clone showing the highest homology with pPLAIIIβ was shown to be overexpressed in Arabidopsis using Agrobacterium. Quantitative polymerase chain reaction was performed to analyze ginseng pPLAIIIβ expression. Phenotypes were observed using a low-vacuum scanning electron microscope. Lignin was stained using phloroglucinol and quantified using acetyl bromide. Results: The PgpPLAIIIβ transcripts were observed in all organs of 2-year-old ginseng. Overexpression of ginseng pPLAIIIβ (PgpPLAIIIβ-OE) in Arabidopsis resulted in small and stunted plants. It shortened the trichomes and decreased trichome number, indicating defects in cell polarity. Furthermore, OE lines exhibited enlarged seeds with less number per silique. The YUCCA9 gene was downregulated in the OE lines, which is reported to be associated with lignification. Accordingly, lignin was stained less in the OE lines, and the expression of two transcription factors related to lignin biosynthesis was also decreased significantly. Conclusion: Overexpression of pPLAIIIβ retarded cell elongation in all the tested organs except seeds, which were longer and thicker than those of the controls. Shorter root length is related to auxinresponsive genes, and its stunted phenotype showed decreased lignin content.

Air-tightness Evaluation of Tube Structures for Super-speed Tube Railway Systems: II. System Test and Parametric Analysis (초고속 열차 시스템을 위한 튜브 구조물의 기밀성 평가 : II. 시스템 실험 및 파라메터 해석)

  • Park, Joo-Nam;Kim, Lee-Hyeon;Nam, Seong-Won
    • Journal of the Korean Society for Railway
    • /
    • v.14 no.2
    • /
    • pp.151-159
    • /
    • 2011
  • This study performed an experimental study for air-tightness performance evaluation of concrete tube structures with joints. The test specimens consist of a continuous concrete tube, a concrete tube with a joint in the middle, and a segmented concrete tube. The test is performed in such a way that the inner pressure of the tube is dropped down to 0.1atm and the increase of the pressure is monitored with time. An equivalent air permeability is then calculated based on the test results. The results show that, as expected, a structure with more joints or bonds tends to be less air-tight. A sensitivity study shows that the system air-tightness performance level becomes higher as either the diameter or the thickness of the tube increases. Moreover, the increase in the diameter or the thickness of the tube makes an effort to enhance the air-tightness more effective.

Absorption Characteristics and Prediction Model of Ginger Powder by Different Drying Methods (건조방법에 따른 생강분말의 흡습특성과 예측모델에 관한 연구)

  • Shin, Hae-Kyoung;Hwang, Sung-Hee;Youn, Kwang-Sup
    • Korean Journal of Food Science and Technology
    • /
    • v.35 no.2
    • /
    • pp.211-216
    • /
    • 2003
  • Absorption characteristics of hot air-. vacuum-, and freeze-dried ginger powder were investigated. Monolayer moisture content as determined by GAB equation was $0.257{\sim}0.540\;H_2O/g$, showing higher significance than BET equation. Absorption enthalpy was calculated based on different drying methods and water activities. Absorption energy decreased with increasing water activity but was not affected by drying method. Isotherm curves showed a typical sigmoid form. Among models applied for predicting equilibrium moisture content, Caurie model was the best fit model for ginger powder, showing the lowest prediction deviation of $1.2{\sim}5.4%$, followed by Henderson then Bradley models. The prediction model equations for the moisture content were established by in(time), water activity, and temperature.

Membrane Processes for Energy Saving in Japan

  • Nakao, Shin-ichi
    • Proceedings of the Membrane Society of Korea Conference
    • /
    • 1994.06a
    • /
    • pp.45-58
    • /
    • 1994
  • Over the last 20 years, membrane separation processes, such as reverse osmosis, ultrafiltration and microfiltration, have been widely adopted by different industries. Commercial uses of membrane have displaced conventional separation processes, such as distillation, evaporation, precoat filter and so on. Membrane separation processes are often more capital and energy efficient when compared with conventional separation processes. Membrane devices and systems are almost always compact and modular. These are the well-known advantages of membrane separation processes. The disadvantage of the membrane process is that the process does not have scale merit and thus the membrane process is suitable for the small and middle size applications. Energy saving is, of course, the biggest advantage of the membrane process, and in many industries the membrane processes are employed because of this reason. Membrane process has other big advantage. In many applications membrane processes provide much higher quality of product than conventional processes. The example is ultrapure water production by membrane processes in semiconductor industry. Conventional technologies never offer such good quality of pure water. If you can obtain both energy saving and higher quality of product at the same time by membrane processes, this is the best application of membrane processes. One example is the concentration of orange juice by membrane, which has already been commercialized in Japan. Comparing with the conventional vacuum evaporation process, juice concentrated by the membrane process has much better taste and flavor and the energy consumption in the membrane process is much less than the evaporation process. In this paper, first membrane separation technology will be classified and then Japanese membrane manufacturers and new modules and devices under development in Japan will be introduced. Fourth energy saving in membrane process will be discussed and finally practical applications of membrane processes in Japan will be shown.

  • PDF

Catalytic Performance of Ionic Liquids in the Synthesis of Glycerol Carbonate from Glycerol and Urea (글리세롤과 요소로부터 글리세롤카보네이트 합성에서 이온성액체의 촉매 특성)

  • Kim, Dong-Woo;Park, Kyung-Ah;Kim, Min-Ji;Park, Dae-Won
    • Korean Chemical Engineering Research
    • /
    • v.51 no.3
    • /
    • pp.347-351
    • /
    • 2013
  • The preparation of glycerol carbonate (GC) from urea through carbonylation with renewable glycerol was investigated by using ionic liquid catalysts. It was found that quaternary ammonium salt and imidazolium salt ionic liquids with a shorter alkyl chain length and higher nucleophilic anion showed better catalytic performance. The effects of reaction temperature, reaction time and degree of vacuum on the reactivity of TBAC catalyst ware discussed. Zinc chloride ($ZnCl_2$) was used as co-catalyst with the ionic liquid catalyst. The mixed catalyst showed a synergy effect on the glycerol conversion and GC yield probably due to the acid-base properties of the catalysts.

Characterization of $V_2O_5$ thin films as a counter electrode for complementary electrochromic devices (상보형 전기변색소자용 $V_2O_5$박막의 대향전극 특성)

  • 조봉희;김영호
    • Electrical & Electronic Materials
    • /
    • v.9 no.7
    • /
    • pp.690-695
    • /
    • 1996
  • We have systematically investigated the characterization of V$_{2}$O$_{5}$ thin films as a counter electrode for lithium based complementary electrochromic devices. The V$_{2}$O$_{5}$ thin films were prepared by thermal vacuum evaporation with varing the substrate temperature and film thickness. In electrochromic devices for smart windows, the WO$_{3}$ thin films with 400-800 nm thickness require to be capable of reversibly injection 10-15 mC/cm$^{2}$ of lithium, which is readily accomplished charge-balanced switching in a V$_{2}$O$_{5}$ thin films with 100-150nm thick. The V$_{2}$O$_{5}$ thin films produces considerably small changes in optical modulation properties in the visible and near infrared region(500-1100 nm) compared to the amorphous WO$_{3}$ thin films on 10-15 mC/cm$^{2}$ of lithium injection and the V$_{2}$O$_{5}$ thin films can therefore act as a counter electrode to WO$_{3}$ in a lithium based complementary clectrochromic devices. After 10$^{5}$ coloration/bleaching switching time, the degradation does not occurs and the devices exhibit a stable optical modulation in V$_{2}$O$_{5}$ thin films. It has shown that the injected lithium ion amounts in crystalline V$_{2}$O$_{5}$ thin films with the same thickness is large by 3-5 mC/cm$^{2}$ of lithium compared to the amorphous thin films in the same driving conditions. Therefore, to optimize the device performance, it is necessary to choose an appropriate film thickness and crystallinity of V$_{2}$O$_{5}$ for amorphous WO$_{3}$ film thickness as a working electrode.

  • PDF

POLYMER SURFACE MODIFICATION WITH PLASMA SOURCE ION IMPLANTATION TECHNIQUE

  • Han, Seung-Hee;Lee, Yeon-Hee;Lee, Jung-Hye;Yoon, Jung-Hyeon;Kim, Hai-Dong;Kim, Gon-ho;Kim, GunWoo
    • Journal of the Korean institute of surface engineering
    • /
    • v.29 no.5
    • /
    • pp.345-349
    • /
    • 1996
  • The wetting property of polymer surfaces is very important for practical applications. Plasma source ion implantation technique was used to improve the wetting properties of polymer surfaces. Poly(ethylene terephtalate) and other polymer sheets were mounted on the target stage and an RF plasma was generated by means of an antenna located inside the vacuum chamber. High voltage pulses of up to -10kV, 10 $\mu$sec, and up to 1 kHz were applied to the stage. The samples were implanted for 5 minutes with using Ar, $N_2,O_2,CH_4,CF_4$ and their mixture as source gases. A contact angle meter was used to measure the water contact angles of the implanted samples and of the samples stored in ambient conditions after implantation. The modified surfaces were analysed with Time-Of-Flight Mass Spectrometer (TOF-SIMS) and Auger Electron Spectroscopy (AES). The oxygen-implanted samples showed extremely low water contact angles of $3^{\circ}C$ compared to $79^{\circ}C$ of unimplanted ones. Furthermore, the modified surfaces were relatively stable with respect to aging in ambient conditions, which is one of the major concerns of the other surface treatment techniques. From TOF-SIMS analysis it was found that oxygen-containing functional groups had been formed on the implanted surfaces. On the other hand, the $CF_4$-implanted samples turned out to be more hydro-phobic than unimplanted ones, giving water contact angles exceeding $100^{\circ}C$ . The experiment showed that plasma source ion implantation is a very promising technique for polymer surface modification especially for large area treatment.

  • PDF

Pulsed Magnetron Sputtering Deposit ion of DLC Films Part I : Low-Voltage Bias-Assisted Deposition

  • Oskomov, Konstantin V.;Chun, Hui-Gon;You, Yong-Zoo;Lee, Jing-Hyuk;Kim, Kwang-Bok;Cho, Tong-Yul;Sochogov, Nikolay S.;Zakharov, Alexender N.
    • Journal of the Korean institute of surface engineering
    • /
    • v.36 no.1
    • /
    • pp.27-33
    • /
    • 2003
  • Pulsed magnetron sputtering of graphite target was employed for deposition of diamond-like carbon (DLC) films. Time-resolved probe measurements of magnetron discharge plasma have been performed. It was shown that the pulsed magnetron discharge plasma density ($∼10^{17}$ $m^{-3}$ ) is close to that of vacuum arc cathode sputtering of graphite. Raman spectroscopy was sed to examine DLC films produced at low ( $U_{sub}$ / < 1 kV) pulsed bias voltages applied to the substrate. It has been shown that maximum content of diamond-like carbon in the coating (50-60%) is achieved at energy per deposited carbon atom of $E_{c}$ =100 eV. In spite of rather high percentage of $sp^3$-bonded carbon atoms and good scratch-resistance, the films showed poor adhesion because of absence of ion mixing between the film and the substrates. Electric breakdowns occurring during the deposition of the insulating DLC film also thought to decrease its adhesion.

Fabrication and Characteristics of ZnO:In Thin Film $NH_3$ Gas Sensor (ZnO:In 박막 $NH_3$ 가스센서의 제작 및 특성)

  • Kim, Jin-Hae;Jun, Choon-Bae;Park, Ki-Cheol
    • Journal of Sensor Science and Technology
    • /
    • v.8 no.3
    • /
    • pp.274-282
    • /
    • 1999
  • The In doped ZnO(ZnO:In)thin films sensitive to $NH_3$ gas were prepared by the double layer depositions of In film by vacuum evaporation and ZnO film by rf magnetron sputtering method onto a $SiO_2$/Si wafer substrate, and subsequent heat treatment process. The structural and electrical characteristics of the ZnO:In thin films were studied as a function of heat treatment temperature by x-ray diffraction, scanning electron microscope and 4 point probing method. And the dependence of the sensitivity, the selectivity and the time response of the thin films on heat treatment temperature was investigated. The thin film heat-treated at $400^{\circ}C$ showed the highest sensitivity of 140% at an operating temperature of $300^{\circ}C$. The sensitivity towards CO, $NO_x$, gases observed in the same temperature.

  • PDF

Synthesis of β-SiC Powder using a Recycled Graphite Block as a Source (그라파이트 블록을 원료로써 재활용한 β-SiC 분말 합성)

  • Nguyen, Minh Dat;Bang, Jung Won;Kim, Soo-Ryoung;Kim, Younghee;Jung, Eunjin;Hwang, Kyu Hong;Kwon, Woo-Teck
    • Resources Recycling
    • /
    • v.26 no.1
    • /
    • pp.16-21
    • /
    • 2017
  • This paper relates to the synthesis of a source powder for SiC crystal growth. ${\beta}-SiC$ powders are synthesized at high temperatures (>$1400^{\circ}C$) by a reaction between silicon powder and carbon powder. The reaction is carried out in a graphite crucible operating in a vacuum ambient (or Ar gas) over a period of time sufficient to cause the Si+C mixture to react and form poly-crystalline SiC powder. End-product characterizations are pursued with X-ray diffraction analysis, SEM/EDS, particle size analyzer and ICP-OES. The purity of the end-product was analyzed with the Korean Standard KS L 1612.