• 한국식품위생안전성학회지
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• 제35권6호
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• pp.602-606
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• 2020

본 연구는 다양한 항생제 내성을 갖는 Salmonella enterica subsp. enterica serovar Typhimurium ATCC 19585 (STWT), S. Typhimurium KCCM 40253 (STKCCM), ciprofloxacininduced S. Typhimurium ATCC 19585 strains (STCIP), and S. Typhimurium CCARM 8009 (STCCARM)에 대한 phage의 흡착 및 용균 특성을 평가하였다. PBST-10, PBST-13, PBST-32, PBST-35, P-22, P-22 B1 phages는 narrow host range를 보였다. 숙주인 STWT, STKCCM, STCIP에 대한 phage의 흡착률은 각각 47-85%, 58-95%, 61-93%였지만, STCCARM에 대한 phage의 흡착률은 14-36%의 낮은 수준을 보였다. STWT, STKCCM, STCIP, STCCARM에 대한 phage burst size는 각각 43-350, 37-530, 66-500, 24-500 plaque-forming unit(PFU)으로 다양하게 관찰되었다. P-22 B1을 제외한 모든 phage는 배양 초기에 STCIP숙주를 효과적으로 저해하였다. 이러한 결과는 항생제 내성균을 저해하기 위해 phage control system 개발에 유용한 정보로 활용될 것이다.

• 한국동물위생학회지
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• 제41권3호
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• pp.133-139
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• 2018

Porcine circovirus type 2 (PCV2) and porcine reproductive and respiratory syndrome virus (PRRSV) are known as significant immunosuppressive viruses in pigs. In this study, we investigated the correlation of PCV2 and PRRSV in enteric lesions of porcine salmonellosis. A total of 64 cases were classified into four pig groups as group A (24 cases, S. Typhimurium), group B (11 cases, S. Typhimurium+PCV2), group C (16 cases, S. Typhimurium+PRRSV) and group D (13 cases, S. Typhimurium+PCV2+PRRSV). Comparing with group A, ulcerative enteritis in large intestine was little more prevalent in the PCV2 infected pigs in group B and D. And lymphoid depletion in gut-associated lymphatic tissue (GALT) of large intestine was also detected in PCV2 positive group B (36.4%) and D (30.8%). According to the results of immunohistochemistry (IHC), PCV2 antigens (83.3%) were more prevalently distributed in the intestinal lesions of porcine salmonellosis than PRRSV antigens (10.3%). PCV2 were also detected in the lymphoid depleted GALT of the large intestine from 7 of the 8 pigs (87.5%), but PRRSV were not found in all cases. It may explain that PCV2 can play a certain immunological role to enhance secondary bacterial infection in porcine alimentary tracts.

• 한국식품영양학회지
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• 제11권1호
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• pp.72-76
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• 1998

세균을 이용한 복귀돌연변이 시험계는 Salmonella typhimurium 히스티딘 요구성 변이주를 이용하여 염기대치환(base change)의 돌연변이를 검출하는 S. typhimurium TA100, TA1535와 frameshift형의 돌연변이를 검출하는 S. Tphimurium TA98, TA1537로 구성되어 있으며, 화학물질의 변이원성에 의하여 히스티딘 비요구성으로 복귀하는 돌연변이를 검토하는 시험계로 널리 이용되고 있다. 시험결과, Yellow pigment는 S. typhimurium에 대한 독성도 나타나지 않았으며, 배지 1$m\ell$당 200$\mu\textrm{g}$ 이하의 모든 농도에서 S-9 Mix(대사활성계)의 유무에 관계없이 S. typhimurium 모든 변이주에서 히스티딘 요구성에 대한 복귀돌연변이를 유발하지 않는 물질로 판단되었다. 이상의 결과로부터 치자황색색소는 S. typhimurium을 이용한 복귀돌연변이 시험에서 유전독성이 없는 물질로 사료되었다.

• 한국동물위생학회지
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• 제33권1호
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• pp.51-57
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• 2010

Salmonella Typhimurium is a virulent pathogen for human and animal. We studied serotypes, biochemical characteristics, and antimicrobial resistance of S. Typhimurium isolated from diseased pigs in Gyeongbuk province over 1998 to 2008. One hundred sixteen isolates were identified as S. Typhimurium by biochemical characteristics and serotypes from 90 farms. The biochemical characteristics of S. Typhimurium isolates was production of $H_2S$, indole-negative, fermentation of mannitol, dulcitol, sorbitol, inositol, rhamnose, and maltose, and ornithine decarboxylase. At antimicrobial susceptibility test, the majority of isolates were highly susceptible to amoxicillin/clavulanic acid, cefepime, ciprofloxacin, while were highly resistant streptomycin, cephalothin, enrofloxacin, nalidixic acid, apramycin, chloramphenicol, tetracycline. The isolates were divided into 65 resistant patterns and 47 of the isolates were shown as a DT104 ASSSuT resistant phenotype.

• 한국동물위생학회지
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• 제37권1호
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• pp.35-43
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• 2014

Salmonellosis has caused heavy losses in swine industry and implications for public health. Recently, the urgent problem of antibiotic resistance due to multidrug-resistant Salmonella spp. has been on the rise. The use of host-specific bateriophages as a biocontrol is one possible alternative. In this study, clinical signs, growth performance, quantification and detection of antigen, histopathological changes of gastrointestinal tracts were analyzed comparatively in weaned piglets according to administration of bacteriophages and challenge with Salmonella (S.) Typhimurium. Piglets challenged with S. Typhimurium after administered with bacteriophages showed reduced clinical signs, higher growth performance, lower bacterial shedding, lower quantificational value of antigens in intestines, higher V/C ratio and higher the number of goblet cells in intestines than piglets administered without bacteriophage and challenged with S. Typhimurium. These results indicate that feeding contained with bacteriophages has effect to prevent infection of S. Typhimurium in weaned piglets and suggest that a use of bacteriophage can be considered a valid antibiotic alternative.

• 생명과학회지
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• 제27권1호
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• pp.72-77
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• 2017

S. Typhimurium SL1344는 성장을 위한 보조인자로 무기철이 요구된다. 철 킬레이트제인 ethylenediamine di-o-hydroxyphenylactic acid (EDDA)가 첨가 된 M9 최소배지에서 S. Typhimurium은 성장에 있어 철 이용이 완전하게 억제된다. 하지만, 세균이나 곰팡이와 같은 미생물들은 철이 부족한 환경에서 제한된 철을 이용하기 위해 사이드로포어를 생산한다. 사람에서 유래한 트랜스페린(hTf)-철 복합체를 M9 배지에 첨가한 조건에서 S. Typhimurium의 사이드로포어 생산은 완전하게 중단되었다. 반면, S. Typhimurium의 성장은 염화철($FeCl_3$)을 첨가한 조건과 동일한 수준으로 유지되었다. 이 결과는 사이드로포어의 생산 없이도 S. Typhimurium이 hTf에 부착된 철을 직접적으로 이용할 수 있다는 것을 알 수 있다. 돌연변이주의 구축과 이를 이용한 분석을 통하여 우리는 세균이 hTf-철 복합체를 직접적으로 이용할 수 있다는 것을 확인할 수 있었다.

• 한국식품위생안전성학회지
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• 제26권3호
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• pp.222-226
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• 2011

본 연구는 세포 내 기생세균인 S. typhimurium의 세포 내 대사과정에서 중요한 역할을 하는 respiratory nitrate reductase의 활성 및 활성 억제 물질인 sodium chlorate, sodium azide, 그리고 sodium cyanide으로 조성된 복합조성물을 이용하여 RAW 264.7 세포에 감염 된 S. typhimurium의 증식억제 효과와, S. typhimurium 감염 마우스에 대한 치료효과를 평 가하기 위하여 수행되었다. 복합조성물을 이용하여 RAW 264.7 세포 감염 S. typhimurium에 대한 증식억제 효과 확인시험을 수행한 결과, 세포 배양 24시간에 대조군과 비교하여 90% 이상의 S. typhimurium의 증식이 억제되었다. 또한, S. typhimurium을 감염시킨 마우스에 복합조성물을 투여한 결과, 70%의 높은 생존율을 보였다. 따라서 본 연구의 결과로부터, sodium chlorate, sodium azide, sodium cyanide로 조성된 복합조성물을 S. tyhimurium에 감염된 마우스에 투여할 경우 S. typhimurium의 증식을 억제하여 감염증상을 치료할 수 있을 것으로 기대된다.

• 한국식품위생안전성학회지
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• 제28권3호
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• pp.234-240
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• 2013

본 연구는 E. coli와 S. typhimurium을 대상으로 orthophenylphenol 20%를 함유한 훈증소독제, Fumagari OPP$^{(R)}$의 살균효과를 평가하기 위해 수행되었다. 예비 시험에서, E. coli와 S. typhimurium의 현탁액 균수는 모두 $4.0{\times}10^8CFU/mL$이었으며, 모든 훈증소독제에 노출시킨 담체의 균수는 모두 평판배지법과 여과법으로 배양한 시험균주 현탁액의 균수의 50%보다 많았다. 또한, 대조 담체로부터 회복된 E. coli와 S. typhimurium 균수는 모두 $3.4{\times}10^6CFU/mL$이었다. 훈증소독제의 살균효과 시험에서는, 훈증소독제를 처리한 담체의 E. coli와 S. typhimurium의 감소 균수는 각각 5.64와 5.26 logCFU/mL로 나타났다. 이상의 결과로부터, 훈증소독제, Fumagari OPP$^{(R)}$은 E. coli와 S. typhimurium에 대해 효과적인 살균력을 갖는 것으로 확인되었으며, 병원성 세균에 오염된 식품재료 및 주방용품의 소독에 적용할 수 있을 것으로 사료된다.

• 한국미생물·생명공학회지
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• 제27권5호
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• pp.419-425
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• 1999

Prophylactic effects of Bifidobacterium longum HY8001, Korean isolate, against Escherichia coli O157:H7 and Salmonella typhimurium DT104 enteric infection were examined at four groups of specific pathogen free(SPF)-ICR mouse for each pathogen. B. longum HY8001+B. typhimurium DT104+B. longum HY8001(BL+ST+BL) group and B. longum HY8001+E. coli O157:H7+B. longum HY8001(BL+E+BL) group were fed with B. longum HY8001 before and after E. coli O157:H7 or s. typhimurium DT104 challenge, while B. longum HY8001+S. typhimurium DT104(BL+ST) and B. longum HY8001+e. coli O157:H7(BL+E) groups were fed with B. longum HY8001 only before E. coli O157:H7 or S. typhimurium DT104 challenge. E. coli O157:H7(E) and S. typhimurium DT104(ST) groups were challenged with each pathogen without B. longum HY8001 administration and control groups were administered with phosphate buffered solution(PBS). After the oral administration with B. longum HY8001(109cfu), th emice were challenged with E. coli O157:H7(2$\times$1010cfu) or S. typhimurium DT104(108cfu) and the mortality rate and the fecal shedding of challenged pathogen were also examined define the reactivity of the B. longum HY8001. Production of toxin neutralizing substance(s) of B. longum HY8001 was determined by cell cytotoxicity assay using Vero cells. Fecal shedding of th eS. typhimurium DT104 was significantly decreased in BL+ST+BL group fed with B. longum HY8--1 before and after challenge(p<0.05), while the fecal shedding s of S. typhimurium DT104 in BL+ST and St groups remained more than 106cfu. the protective effect of the B. longum HY8001 against E. coli O157:H7 was significantly high only in BL+E+BL group fed with b. longum Hy8001 before and after E. coli O157:H7 challenge from the result of fecal E. coli O157:H7 isolation rate, mortality rate, and intestinal contents culture to detect E. coli O157:H7. the mortality rate of the BL+e and E groups. The cytopathic effect (CPE) of the Vero cytotoxin (Shiga like toxin I & II) in Vero cell was neutralized in B. longum HY8001 culture supernatant added wells which indicate the presence of soluble Vero cytotxin neutralizing substance(s) in B. longum HY8001 culture suprnatant.

• 대한한방내과학회지
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• 제36권2호
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• pp.165-179
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• 2015

Objectives: This experiment investigated the antimicrobial activity of Hwangryunheadok-tang and extracts of Scutellariae radix , Phellodendri cortex , Coptis rhizome , and Gardenia jasminoides against Salmonella typhimurium . Methods: After spreading S. typhimurium on a bacterial culture medium plate, antimicrobial activity was tested by dripping diluted Hwangryunheadok-tang or extracts of Scutellariae radix , Phellodendri cortex , Coptis rhizome , or Gardenia jasminoides (80 μl, diluted 100, 50, 10, and 1%) onto the plate, followed by culture for 16 to 72 hours. The minimal inhibitory concentration (MIC) was tested by dripping the minimum dilution that showed antimicrobial activity (80, 60, 40, and 20 μl) and measuring the density. The antimicrobial activity of Hwangryunheadok-tang and four extracts showed continuous antibacterial activity against S. typhimurium throughout the experiment. Result: 1. S. typhimurium . (Standard Microorganism, ATCC) 1) The Hwangryunheadok-tang and extracts of Scutellariae radix , Phellodendri cortex , and Coptis rhizome showed antibacterial activity in the undiluted solutions and in 50% dilutions. Gardenia jasminoides extract showed potency only in the undiluted solution. The antimicrobial potency of the undiluted solution was increased when the volume of inoculation was increased, but no difference was noted when the culture time was extended. All the extracts showed antimicrobial potency against S. typhimurium . 2. S. typhimurium isolated from diarrhea patients 1) When compared to the standard microorganism experiment on S. typhimurium , the MICs of the five extracts were increased. However, whereas the antimicrobial potency of doxycycline was lost entirely against bacteria isolated from patients with diarrhea, the antimicrobial potency of all the extracts was diminished but did not disappear. 2) The antimicrobial activity of Hwangryunheadok-tang and four extracts was continuous even when the culture time was extended to 16, 24, and 72 hours. Conclusions: The Hwangryunheadok-tang and four kinds of extracts have antimicrobial activity against S. typhimurium . Comparison of a standard microorganism with S. typhimurium isolated from diarrhea patients showed that the antimicrobial activity of all the extracts was better than that of antibiotics. Further studies should focus on the value and benefits of Hwangryunheadok-tang , and the four kinds of extracts as clinical treatments.