Browse > Article
http://dx.doi.org/10.13103/JFHS.2013.28.3.234

Bactericidal Efficacy of Fumagari OPP®, Fumigant Against Escherichia coli and Salmonella typhimurium  

Park, Eun-Kee (Department of Medical Humanities and Social Medicine, College of Medicine, Kosin University)
Kim, Yongpal (Elkahmco Bio Co., Ltd.)
Yu, Eun-Ah (Tongyeong National Quarantine Station, Ministry of Health & Welfare)
Yoo, Chang-Yeol (Department of Computer Information, Gyeongnam Provincial Namhae College)
Choi, Hyunju (Elderly Life Redesign Institute, Department of Biomedical Laboratory Science, Inje University)
Kim, Suk (Research Institute of Life Sciences, College of Veterinary Medicine, Gyeongsang National University)
Lee, Hu-Jang (Research Institute of Life Sciences, College of Veterinary Medicine, Gyeongsang National University)
Publication Information
Journal of Food Hygiene and Safety / v.28, no.3, 2013 , pp. 234-240 More about this Journal
Abstract
This test was performed to evaluate the bactericidal efficacy of Fumagari OPP$^{(R)}$, fumigation disinfectant, containing 20% ortho-phenylphenol against Escherichia coli (E. coli) and Salmonella typhimurium (S. typhimurium). In preliminary tests, both E. coli and S. typhimurium working culture suspension number (N value) was $4.0{\times}10^8$ CFU/mL. And all of the colony numbers on the carriers exposed the fumigant (n1, n2, n3) were higher than 0.5N1 (the number of bacterial test suspentions by pour plate method), 0.5N2 (the number of bacterial test suspentions by filter membrane method) and 0.5N1, respectively. In addition, the mean number of bacteria recovered on the control-carriers (T value) was $3.4{\times}10^6$ CFU/mL. In the bactericidal effect of the fumigant, the reduction number of S. typhimurium and E. coli (d value) was 5.26 and 5.64 logCFU/mL, respectively. According to the French standard for the fumigant, the d value for the effective bactericidal fumigant should be over than 5 logCFU/mL. With the results of this study, Fumagari OPP$^{(R)}$ has an effective bactericidal activity, then the fumigant can be applied to disinfect food materials and kitchen appliances contaminated with pathogenic bacteria.
Keywords
Fumigant; disinfectant; ortho-phenylphenol; Escherichia coli; Salmonella typhimurium;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Himathongkham, S., Nuanualsuwan, S., Riemann, H. and Cliver, D.O.: Reproduction of Escherichia coli O157:H7 and Salmonella typhimurium in artificially contaminated alfalfa seeds and mung beans by fumigation with ammonia. J. Food Prot. 64, 1817-1819 (2001).
2 Sapers, G.M., Walker, P.N., Sites, J.E., Annous, B.A. and Eblen, D.R.: Vapor-phase decontamination of apples inoculated with Escherichia coli. J. Food Sci. 68, 1003-1007 (2003).   DOI   ScienceOn
3 Association Francaise de Normalisation (AFNOR): Methods of airborne disinfection of surfaces - Determination of bactericidal, fungicidal, yeasticidal and sopricidal activity. French standard NF T 72-281, AFNOR, Saint-Denis, pp. 6-22 (2009).
4 Mills-Robertson, F.C., Tay, S.C.K., Duker-Eshun, G., Walana, W. and Badu, K.: In vitro antimicrobial activity of ethanolic fractions of Cryptolepis sanguinolenta. Ann. Clin. Microbiol. Antimicrob. 11, 16 (2012).   DOI
5 Brashears, M.M., Amezquita, A. and Stratton J.: Validation of methods used to recover Escherichia coli O157:H7 and Salmonella spp. subjected to stress conditionst. J. Food Prot. 64, 1466-1471 (2001).
6 Tanny, G.B., Mirelman, D. and Pistole, T.: Improved filtration technique for concentrating and harvesting bacteria. Appl. Environ. Microbiol. 40, 269-273 (1980).
7 Mahmoud, B.S.M. and Linton, R.H.: Inactivation kinetics of inoculated Escherichia coli O157:H7 and Salmonella enterica on lettuce by chlorine dioxide gas. Food Microbiol. 25, 244-252 (2008).   DOI   ScienceOn
8 Korsak, N., Jacob, B., Groven, B., Etienne, G., China, B., Ghafir, Y. and Daube, G.: Salmonella contamination of pigs and pork in an integrated pig production system. J. Food Prot. 66, 1126-1133 (2003).
9 Garland, J.B., Frye, J.G., Gray, J.T., Berrang, M.E., Harrison, M.A., Cray, P.J.F.: Transmission of Salmonella enterica serovar Typhimurium in poultry with and without antimicrobial selective pressure. J. Appl. Microbiol. 101, 1301-1308 (2006).   DOI   ScienceOn
10 Sharan, R., Chhibber, S. and Reed, R.H.: A murine model to study the antibacterial effect of copper on infectivity of Salmonella Enterica Serovar Typhimurium. Int. J. Environ. Res. Public Health, 8, 21-36 (2011).
11 Huang, J.J., Hu, H.Y., Wu, Y.H., Wei, B. and Lu, Y.: Effect of chlorination and ultraviolet disinfection on tetA-mediated tetracycline resistance of Escherichia coli. Chemosphere, 90, 2247-2253, (2013).   DOI   ScienceOn
12 Trinetta, V., Morgan, M.T. and Linton, R.H.: Use of highconcentration- short-time chlorine dioxide gas treatments for the inactivation of Salmonella enterica spp. inoculated onto Roma tomatoes. Food Microbiol. 27, 1009-1015 (2010).   DOI   ScienceOn
13 Sheldon, A.T. Jr.: Antiseptic "resistance": real or perceived threat? Clin. Infect. Dis. 40, 1650-1656 (2005).   DOI   ScienceOn
14 Loaharanu, P.: Irradiated foods. 5th Ed. American Council on Science and Health, New York, pp. 7-8 (2003).
15 Coelhan, M., Bromig, K.H., Glas, K. and Roberts, A.L.: Determination and levels of the biocide ortho-Phenylphenol in canned beers from different countries. J. Agric. Food Chem. 54, 5731-5735 (2006).   DOI   ScienceOn
16 Formato, A., Naviglio, D., Pucillo, G.P. and Nota, G.: Improved fumigation process for stored foodstuffs by using phosphine in sealed chambers. J. Agric. Food Chem. 60, 331- 338 (2012).   DOI   ScienceOn
17 Turkmani, A., Psaroulaki, A., Christidou, A., Samoilis, G., Mourad, T.A., Tabaa, D. and Tselentis, Y.: Uptake of ciprofloxacin and ofloxacin by 2 Brucella strains and their fluoroquinolone- resitant variants under different conditions. An in vitro study. Dign. Microbiol. Infect. Dis. 59, 447-451 (2007).   DOI   ScienceOn
18 Ryu, S.H., Lee, J.H., Park, S.H., Song, M.O., Park, S.H., Jung, H.W., Park, G.Y., Choi, S.M., Kim, M.S., Chae, Y.Z., Park, S.G. and Lee, Y.K.: Antimicrobial resistance profiles among Escherichia coli strains isolated from commercial and cooked foods. Int. J. Food Microbiol. 159, 263-266 (2012).   DOI   ScienceOn
19 Zhao, T., Doyle, M.P., Zhao, P., Blake, P. and Wu, F.M.: Chlorine inactivation of Escherichia coli 0157:H7 in water. J. Food Prot. 64, 1607-1609 (2001).
20 Whitehead, R.N., Overton, T.W., Kemp, C.L. and Webber, M.A.: Exposure of Salmonella entericSerovar Typhimurium to high level biocide challenge can select multidrug resistant mutants in a single step. PLoS ONE, 6, e22833 (2011).   DOI
21 Russell, A.D.: Biocide use and antibiotic resistance: the relevance of laboratory findings to clinical and environmental situations. Lancet Infect. Dis. 3, 794-803 (2003).   DOI   ScienceOn
22 Erkmen, O.: Antimicrobial effects of hypochlorite on Escherichia coli in water and selected vegetables. Foodborne Pathog. Dis. 7, 953-958 (2010).   DOI   ScienceOn
23 Cleaveland, S., Laurenson, M.K. and Taylor. L.H.: Diseases of humans and their domestic mammals: pathogen characteristics, host range and the risk of emergence. B. Biol. Sci. 356, 991-999 (2001).   DOI   ScienceOn
24 Sorrells, K.M., Speck, M.L. and Warren, J.A.: Pathogenicity of Salmonella gallinarumafter metabolic injury by freezing. Appl. Environ. Microbiol. 19, 39-43 (1970).
25 Kim, G.S., Kim, D.H., Lim, J.J., Lee, J.J., Han, D.Y., Lee, W.M., Jung, W.C., Min, W.G., Won, C.G., Rhee, M.H., Lee, H.J. and Kim, S.: Biological and antibacterial activities of the natural herb Houttuynia cordata water extract against the intracellular bacterial pathogen Salmonella within the raw 64.7 macrophage. Biol. Pharm. Bull. 31, 2012-2017 (2009).
26 Kim, D.H., Lim, J.J., Lee, J.J., Jung, W.C., Shin, H.J., Lee, H.J., Kim, G.S. and Kim, S.: Antibacterial and therapeutic effects of Houttuynia cordata ethanol extract for murine salmonellosis. Kor. J. Environ. Agricul. 27, 156-162 (2008).   과학기술학회마을   DOI   ScienceOn
27 Valle, E. and Guiney, D.G.: Characterization of salmonellainduced cell death in human macrophage-like THP-1 cells. Infect. Immun. 73, 2835-2840 (2005).   DOI   ScienceOn
28 Beuchat, L.R. and Heaton, E.K.: Salmonella survival on pecans as influenced by processing and storage conditions. Appl. Environ. Micobiol. 29, 795-801 (1975).
29 Katsuda, K., Kohmoto, M., Kawashima, K. and Tsunemitsu, H.: Frequency of enteropathogen detechtion in sucking and weaned pigs with diarrhea in Japan. J. Vet. Diagn. Invest. 18, 350-354 (2006).   DOI   ScienceOn