Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Pattern of antimicrobial resistance and biochemical characteristics of Salmonella Typhimurium isolated from diseased pigs in Gyeongbuk province

경북지방 환돈에서 분리한 Salmonella Typhimurium의 생화학적 성상 및 약제내성 패턴

  • 김성국 (경상북도가축위생시험소) ;
  • 엄현정 (경상북도가축위생시험소) ;
  • 김순태 (경상북도가축위생시험소) ;
  • 장영술 (경상북도가축위생시험소) ;
  • 조민희 (경상북도가축위생시험소)
  • Received : 2010.02.19
  • Accepted : 2010.03.25
  • Published : 2010.03.30
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Abstract

Salmonella Typhimurium is a virulent pathogen for human and animal. We studied serotypes, biochemical characteristics, and antimicrobial resistance of S. Typhimurium isolated from diseased pigs in Gyeongbuk province over 1998 to 2008. One hundred sixteen isolates were identified as S. Typhimurium by biochemical characteristics and serotypes from 90 farms. The biochemical characteristics of S. Typhimurium isolates was production of $H_2S$, indole-negative, fermentation of mannitol, dulcitol, sorbitol, inositol, rhamnose, and maltose, and ornithine decarboxylase. At antimicrobial susceptibility test, the majority of isolates were highly susceptible to amoxicillin/clavulanic acid, cefepime, ciprofloxacin, while were highly resistant streptomycin, cephalothin, enrofloxacin, nalidixic acid, apramycin, chloramphenicol, tetracycline. The isolates were divided into 65 resistant patterns and 47 of the isolates were shown as a DT104 ASSSuT resistant phenotype.

Keywords

References

  1. 김규태, 정병열, 김봉환. 2003. 경북지역 가축에서 분리한 Salmonella 속균의 혈청형 분포 및 약제감수성. 한국수의공중보건학회지 27(1): 47-52.
  2. 김상윤, 이희무, 김신 등. 2001. 경북지역 가축에서 Salmonella 속균 감염증에 대한 역학적 특성. 한국가축위생학회지 24(1): 51-68.
  3. 이우원, 정병열, 이강록 등. 2009. 소와 돼지 유래 Salmonella 속균의 혈청형 및 약제감수성. 한국가축위생학회지 32(1): 49-59.
  4. 정병열, 박경미, 김봉환. 2004. 환돈 유래 Salmonella Typhimurium의 약제감수성 양상. 한국수의공중보건학회지 28(2): 109-115.
  5. 최원종, 정지헌, 원호근 등. 2008. 도축돈에서 분리한 살모넬라의 혈청형 및 유전형. 한국가축위생학회지 31(1):1-16.
  6. 최원필, 이희석, 여상건 등. 1989. 우, 돈에서 분리한 Salmonella 유래 R Plasmid의 유전학적 및 분자생물학적 성상에 관한 연구 II. R Plasmid의 비적합성 및 plasmid profile. 대한수의학회지 29(2): 139-147.
  7. Blackburn BO, Schlater LK. Swanson MR. 1984. Antibiotic resistance of members of the genus Salmonella isolated from chickens, turkeys, cattle, and swine in the United States during October 1981 through September 1982. Am J Vet Res 45:1245-1249.
  8. Clinical and laboratory standards institute. 2009. Performance standards for antimicrobial disk susceptibility test; Approved standard, 10th Ed. 29(1): M02-A10.
  9. Ewing WH. 1986. Edwards and Ewing's identification of Enterobacteriaceae. 4th Ed. Elsevier, Amsterdam: 181-318.
  10. Fales WH, Maddox CW, Oakman JK. 1990. Antimicrobial susceptibility of Salmonella Choleraesuis from Missouri swine. In 33d Annu Meet AAVLD, Denver, Colo.
  11. Farzan A, Friendship RM, Poppe C, Martin L, Dewey CE, Funk J. 2008. Molecular epidemiology and antimicrobial resistance of Salmonella Typhimurium DT104 on Ontario swine farms. Can J Vet Res 72(2): 188-194.
  12. George M, Garrity GM. 2005. Bergey's Manual of systematic bacteriology. Michigan State University. East Lansing. 764-799.
  13. Hong Y, Liu T, Lee MD, Hofacre CL, Maier M, White DG, Ayers S, Wang L, Berghaus R, Maurer JJ. 2008. Rapid screening Salmonella enterica serovars Enteritidis, Hadar, Heidelberg and Typhimurium using a serologically correlative allelotyping PCR targeting the O and H antigen alleles. BMC Micbiol 8: 178.
  14. Jung BY, Lee WW, Lee HS, Kimi OK, Kim BH. 2001. Prevalence of Salmonella in slaughter pigs in South Korea. Proceedings of the 4th International symposium on the epidemiology and control of Salmonella and other food borne pathogens in pork. Leipzig, Germany: 162- 173.
  15. Poppe C, Smart N, Khakhria R, Johnson W, Spika J, Prescott J. 1998. Salmonella Typhimurium DT104: A virulent and drug-resistant pathogen. Can Vet J 39: 559-565.
  16. Schwartz KJ, Taylor DJ, Straw BE, D'Allaire S, Mengeling WL. 1999. Diseases of swine. Iowa state University press. Ames, Iowa. 535-551.
  17. Timoney JF, Gillespie JH, Scott FW, Barlough JE. 1988. Hagan and Bruner's Microbiology and infectious diseases of domestic animals. Cornell University press. New York: 74-88.
  18. Threlfall EJ, Frost JA, Ward LR, Rowe B. 1994. Epidemic in cattle and humans of Salmonella Typhimurium DT104 with chromosomally integrated multiple drug resistance. Vet Rec 134: 577. https://doi.org/10.1136/vr.134.22.577
  19. Threlfall EJ, Ward LR, Rowe B. 1997. Increasing incidence of resistance to trimethoprim and ciprofloxacin in epidemic Salmonella Typhimurium DT104 in England and Wales. Eurosurveillance 2: 81-84.
  20. Van Duijkeren E, Wannet WJ, Houwer DJ, Van Pelt W. 2002. Serotype and phage type distribution of Salmonella strains isolated from humans, cattle, pigs, and chickens in the Netherlands from 1994 to 2001. J Clin Microbiol 40 (11): 2980-3985.
  21. Wilcock BP, Armstrong CH, Olander HJ. 1976. The significance of the serotype in the clinical and pathologic features of naturally occurring porcine salmonellosis. Can J Comp Med 40: 80-88.
  22. Yang SJ, Park KY, Kim SH, No KM, Besser TE, Yoo HS, Kim SH, Lee BK, Park YH. 2002. Antimicrobial resistance in Salmonella enterica srrovar Enteritidis and Typhimurium isolated from animals in Korea: comparison of phenotypic and genotypic resistance characterization. Vet Microbiol 86(4): 295-301. https://doi.org/10.1016/S0378-1135(02)00009-3